Analysis of Aspect Ratio in a Miniature Rectangle Channel for Low Frictional Resistance

We conducted a theoretical investigation of the cross-sectional aspect ratio of a rectangular channel to have sufficiently low frictional resistance under less than 150 of the Reynolds number. From the theoretical consideration, it was clarified that 3.40 or more is recommended as a criterion for determining the aspect ratio. This addresses the problem of determining the interval of rectangle channels, installed in a plate reactor. There is a concern that the real system does not follow the analytical solution, assuming laminar flow, since the higher aspect ratio leads to disturbances of the flow such as the emergence of vortices. However, in the channel’s volume range of (W × H × L) = (7.0 mm × 0.38 mm × 0.26 m), such a turbulence was not observed in the detailed numerical calculation by CFD, where both calculation results were in agreement to within 3% accuracy. Moreover, even in an experimental system with a surface roughness of ca. 7%, friction resistance took agreement within an accuracy of ±30%.

Inflation instability in the lung: an analytical model of a thick-walled alveolus with wavy fibres under large deformations

Inflation of hollow elastic structures can become unstable and exhibit a runaway phenomenon if the tension in their walls does not rise rapidly enough with increasing volume. Biological systems avoid such inflation instability for reasons that remain poorly understood. This is best exemplified by the lung, which inflates over its functional volume range without instability. The goal of this study was to determine how the constituents of lung parenchyma determine tissue stresses that protect alveoli from instability-related overdistension during inflation. We present an analytical model of a thick-walled alveolus composed of wavy elastic fibres, and investigate its pressure–volume behaviour under large deformations. Using second-harmonic generation imaging, we found that collagen waviness follows a beta distribution. Using this distribution to fit human pressure–volume curves, we estimated collagen and elastin effective stiffnesses to be 1247 kPa and 18.3 kPa, respectively. Furthermore, we demonstrate that linearly elastic but wavy collagen fibres are sufficient to achieve inflation stability within the physiological pressure range if the alveolar thickness-to-radius ratio is greater than 0.05. Our model thus identifies the constraints on alveolar geometry and collagen waviness required for inflation stability and provides a multiscale link between alveolar pressure and stresses on fibres in healthy and diseased lungs.

Sessile drops in weightlessness: an ideal playground for challenging Young’s equation

Sessile drop creation in weightlessness is critical for designing scientific instruments for space applications and for manipulating organic or biological liquids, such as whole human blood or DNA drops. It requires perfect control of injection, spreading, and wetting; however, the simple act of creating a drop on a substrate is more complex than it appears. A new macroscopic model is derived to better understand this related behavior. We find that, for a given set of substrate, liquid, and surrounding gas properties, when the ratio of surface free energies to contact line free energy is on the macroscopic scale, the macroscopic contact angle can vary at static equilibrium over a broad volume range. It can increase or decrease against volume depending on the sign of this ratio up to an asymptotic value. Consequently, our model aims to explore configurations that challenge the faithful representativity of the classical Young’s equation and extends the present understanding of wetting.

In vitro and in vivo interaction of caspofungin with isavuconazole against Candida auris planktonic cells and biofilms

The in vitro and in vivo efficacy of caspofungin was determined in combination with isavuconazole against Candida auris. Drug–drug interactions were assessed utilising the fractional inhibitory concentration indices (FICIs), the Bliss independence model and an immunocompromised mouse model. Median planktonic minimum inhibitory concentrations (pMICs) of 23 C. auris isolates were between 0.5 and 2 mg/L and between 0.015 and 4 mg/L for caspofungin and isavuconazole, respectively. Median pMICs for caspofungin and isavuconazole in combination showed 2–128-fold and 2–256-fold decreases, respectively. Caspofungin and isavuconazole showed synergism in 14 out of 23 planktonic isolates (FICI range 0.03–0.5; Bliss cumulative synergy volume range 0–4.83). Median sessile MICs (sMIC) of 14 biofilm-forming isolates were between 32 and >32 mg/L and between 0.5 and >2 mg/L for caspofungin and isavuconazole, respectively. Median sMICs for caspofungin and isavuconazole in combination showed 0–128-fold and 0-512-fold decreases, respectively. Caspofungin and isavuconazole showed synergistic interaction in 12 out of 14 sessile isolates (FICI range 0.023–0.5; Bliss cumulative synergy volume range 0.13–234.32). In line with the in vitro findings, synergistic interactions were confirmed by in vivo experiments. The fungal kidney burden decreases were more than 3 log volumes in mice treated with combination of 1 mg/kg caspofungin and 20 mg/kg isavuconazole daily; this difference was statistically significant compared with control mice (p<0.001). Despite the favourable effect of isavuconazole in combination with caspofungin, further studies are needed to confirm the therapeutic advantage of this combination when treating an infection caused by C. auris.

Data analytics to investigate the cohort of injection wells with earthquakes in Oklahoma

The number of recorded earthquakes in Oklahoma has substantially increased during the last few decades, a trend that coincides with the increases in the injected volume in underground injection control (UIC) wells. Several studies have suggested the existence of spatial and temporal links between earthquakes and injection wells. However, creating a spatial connection between the earthquakes and UIC wells requires making a prior assumption about the radius of induced seismicity. In this study, we use intrinsic features of the UIC wells to find the cohort of wells with associated earthquakes, based on the level of activity and proximity of the wells to the events. For this purpose, a hybrid genetic algorithm–K-means (GA-K-means) algorithm was applied over UIC wells, and the geographical representation of the clustered wells was co-visualized with earthquake data to determine wells with induced seismic activities. The analysis was performed every year since 2002, and the most critical attributes to distinguish the behavior of wells were identified. The analysis showed a distinct change in cluster identifiers before the year 2010, which is believed to be the beginning of increased seismic activities, compared to later dates. Our approach was able to group the earthquake-associated wells from the rest of the data, and centroid analysis of these wells helped us identify the critical pressure and cumulative volume range that result in induced seismicity. These findings can be used as guidelines for designing safer injection sites for sustainable energy production in Oklahoma.

Altered Airway Mechanics in the Context of Obesity and Asthma

The obesity epidemic is causing a rise in asthma incidence due to the appearance of an obesity-specific late-onset non-allergic (LONA) phenotype. We investigated why only a subset of obese participants develop LONA asthma by determining how obesity, both alone and in combination with LONA asthma, affects the volume dependence of respiratory system impedance. We also determined how obesity and asthma affect impedance during and following challenge with the PC20 dose of methacholine. We found during passive exhalation that all obese participants, in contrast to lean controls and lean asthmatics, experienced similarly profound elevations in lung elastance as they approached functional residual capacity. We also found, however, that the LONA asthmatics had a greater negative dependence of airway resistance on lung volume over the middle of the volume range compared to the other groups. Methacholine challenge with the PC20 dose led to comparable changes in respiratory system impedance in the 4 study groups, but the doses themselves were substantially lower in both obese and lean asthmatic participants compared to obese and lean controls. Also, the obese LONA asthmatics had higher breathing frequencies and lower tidal volumes post challenge compared to the other participants. Taken together, these results suggest that all obese individuals experience substantial lung collapse as they approach FRC, presumably due to the weight of the chest wall. It remains unclear why obese LONA asthmatics are hyperresponsive to methacholine while obese non-asthmatic individuals are not.

Reverse Pneumatic Artificial Muscles for Application in Low-Cost Artificial Respirators

One of the main challenges associated with mechanical ventilators is their limited availability in pandemics and other emergencies. Therefore, there is a great demand for mechanical ventilators to address this issue. In this work, we propose a low-cost, portable, yet high-performance design for a volume-controlled mechanical ventilator. We are employing pneumatic artificial muscles, such as air cylinders, in the reverse mode of operation to achieve mechanical ventilation. The current design of the device can operate in two modes: controlled mode and assisted mode. Unlike most ICU ventilators, our device does not need a high-pressure air pipeline to operate. With the current design, mechanical ventilation for respiration rate ranging from 10 b/min to 30 b/min with a tidal volume range of 150 mL to 1000 mL and I:E ratio of 1:1 to 1:5 can be performed. We achieved a total cost of less $400 USD to make one device. We estimate the device to cost less than $250 USD when produced in larger volumes.

The Babbage Field, Block 48/2a, UK North Sea

The Babbage gas field was discovered in 1988 by exploration well 48/2-2 which drilled into the Permian-age lower Leman Sandstone Formation below a salt wall. Seismic imaging is compromised by the presence of this salt wall, which runs east–west across the southern part of the structure, creating uncertainties in depth conversion and in the in-place volumes. Pre-stack depth migration with beam and reverse time migrations appropriate for the complex salt geometry provided an uplift in subsalt seismic imaging, enabling the development of the field, which is located at the northern edge of the main reservoir fairway in a mixed aeolian–fluvial setting. Advances in artificial fracturing technology were also critical to the development: in this area, deep burial is associated with the presence of pore-occluding clays, which reduce the reservoir permeability to sub-millidarcy levels. The Babbage Field was sanctioned in 2008, based on an in-place volume range of 248–582 bcf; first production was in 2010. It produces from five horizontal development wells that were artificially fracced to improve deliverability of gas from the tight matrix. None of the wells has drilled the gas–water contact, which remains a key uncertainty to the in-place volumes, along with depth-conversion uncertainty below the salt wall.