scholarly journals Antioxidative Properties and Cytotoxic Activity Against Colon Cancer Cell WiDr of Rhizopus Oryzae and Rhizopus Oligosporus-Fermented Black Rice Bran Extract

2018 ◽  
Vol 6 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Kristian Edo Zulfafamy ◽  
Ardiansyah Ardiansyah ◽  
Slamet Budijanto
Keyword(s):  
Colon Cancer ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Rice Bran ◽  
Antiproliferative Activity ◽  
Rhizopus Oryzae ◽  
Colon Cancer Cell ◽  
Total Phenolic ◽  
Black Rice ◽  
Black Rice Bran

Black rice bran (BRB) was reported to have several bioactive compounds which can potentially as antioxidant and reduce the colon cancer incidence. Fermentation was reported to be able to increase bioavailability through deliberating the bioactive compounds from the cellular matrix. The aim of this study was to improve the bioavailability of BRB bioactive compounds which offer beneficial health properties as antiproliferative against colon cancer cell WiDr and confer antioxidant activity by fermentation. The study was conducted in 4 groups of different condition: non-fermented, the use of R. oryzae, R. oligosporus, and combination of R. oryzae + R. oligosporus for fermentation starter, respectively. BRB fermentation was conducted in 72 and 96 hours. Each of fermented BRB (FBRB) was then extracted by using 70% ethanol and methanol, respectively. Extracts derived from FBRB were evaluated for their availability of total phenolic content (TPC) by using total phenolic analysis, antioxidative activity by using DPPH and FRAP assay, and antiproliferative properties toward colon cancer WiDr by using MTT assay. The result of these studies showed that an increased amount of TPC of the ethanolic and methanolic extract of FBRB when compared to non-fermented BRB (NFBRB). Consequently, DPPH and FRAP antioxidant capacity of FBRB was higher than that of NFBRB. The antiproliferative activity of FBRB against colon cancer cells, WiDr, was also shown to be higher activity than that of NFBRB (As shown on IC50 extract). This results indicated that BRB could optimally increase its antioxidant and antiproliferative activity by fermentation.

In vitro antiproliferative activity against human colon cancer cell lines of representative 4-thiazolidinones. Part I

2005 ◽  
Vol 15 (17) ◽  
pp. 3930-3933 ◽  
Author(s):  
Rosaria Ottanà ◽  
Stefania Carotti ◽  
Rosanna Maccari ◽  
Ida Landini ◽  
Giuseppa Chiricosta ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Human Colon ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines

scholarly journals Riceberry rice bran protein hydrolyzed fractions induced apoptosis, senescence and G1/S cell cycle arrest in human colon cancer cell lines

2021 ◽  
Author(s):  
Vichugorn Wattayagorn ◽  
Mesayamas Kongsema ◽  
Sukuntaros Tadakittisarn ◽  
Pramote Chumnanpuen
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Rice Bran ◽  
Metastatic Cancer ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Metastatic Cancer Cell ◽  
Ht 29

Riceberry rice bran is the part of rice that has been scrubbed out during coloring process. There are various health benefits with high protein content and antioxidant ability. The hydrolyzed rice bran consists of diverse peptides that provide various bioactive properties. This work aimed to study the effect of hydrolyzed riceberry rice bran extracted on colon cancer cell lines (HT- 29 and SW- 620) compared to normal cell (PCS- 291- 010). The MTT assay result showed that our extract has less cytotoxicity on normal cell (PCS-291-010, IC50 = 6,680.00 µg/ml) compared to the colon cancer cell lines and has more effect on metastatic cancer cell line (SW-620, IC50 = 5,492.31 µg /ml) than non-metastatic cancer cell line (HT-29, IC50 =6,040.76 µg/ml). According to the DNA fragmentation pattern analysis, the ladder pattern indicated that the rice bran extract can induce the apoptosis process in SW-620 cell line. Confirmed the pattern of apoptotic cell by AO/PI double stain test and quantified apoptotic cells by Annexin V. For the cell senescence analysis, SA-β-gal staining technique was performed at 24 h after treatments, HT-29 reached maximum senescence rate at 85.74% while SW-620 had only 17.23% of senescence. And a result of cell cycle analysis, HT-29 were decreased the number of cells in S, M/G2 phase, and increased the number of cells in G0/G1 phase. Furthermore > 50 kDa peptide fraction separated from HRBE has a potent anti-cancer cells (SW-620, IC50 = 4,908 µg/ml). In conclusion, the hydrolyzed riceberry rice bran extract can inhibit colon cancer cell lines with less effect on normal cell. The extracts could induce apoptosis process in metastatic cancer cell and induce senescence process in non-metastatic cancer cell. This observed information will be useful and applicable for medical research and colon cancer treatment in the future.

Inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from Poncirus trifoliata (L.) Raf.

2007 ◽  
Vol 15 (14) ◽  
pp. 4923-4932 ◽  
Author(s):  
G.K. Jayaprakasha ◽  
K.K. Mandadi ◽  
Shibu M. Poulose ◽  
Y. Jadegoud ◽  
G.A. Nagana Gowda ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Antioxidant Activity ◽  
Cell Growth ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Colon Cancer Cell ◽  
Poncirus Trifoliata ◽  
Cancer Cell Growth

In Vitro Antiproliferative Activity of Fresh Pineapple Juices on Ovarian and Colon Cancer Cell Lines

2015 ◽  
Vol 21 (3) ◽  
pp. 353-364 ◽  
Author(s):  
Madihah Binti Abdul Gani ◽  
Rozita Nasiri ◽  
Javad Hamzehalipour Almaki ◽  
Fadzilah Adibah Abdul Majid ◽  
Mohsen Marvibaigi ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines

scholarly journals Walnut Phenolic Extract and Its Bioactive Compounds Suppress Colon Cancer Cell Growth by Regulating Colon Cancer Stemness

Nutrients ◽  
2016 ◽  
Vol 8 (7) ◽  
pp. 439 ◽  
Author(s):  
Jisoo Lee ◽  
Yoo-Sun Kim ◽  
JaeHwan Lee ◽  
Seung Heo ◽  
Kook Lee ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Colon Cancer Cell ◽  
Cancer Cell Growth ◽  
Cancer Stemness ◽  
Phenolic Extract

scholarly journals Comparison of the Antiproliferative Activity of Two Antitumour Ruthenium(III) Complexes With Their Apotransferrin and Transferrin-Bound Forms in a Human Colon Cancer Cell Line

1996 ◽  
Vol 3 (1) ◽  
pp. 15-23 ◽  
Author(s):  
F. Kratz ◽  
B. K. Keppler ◽  
M. Hartmann ◽  
L. Messori ◽  
M. R. Berger
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Cancer Cell Line ◽  
Human Colon ◽  
Colon Cancer Cell Line ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer

Two ruthenium(III) complexes, namely trans-indazolium[tetrachlorobis(indazole)- ruthenate(III)], HInd[RuInd2Cl4] and trans-imidazolium[tetrachlorobis(imidazole)- ruthenate(III)], HIm[RuIm2Cl4] exhibit high anticancer activity in an autochthonous colorectal carcinoma model in rats. Recently, it has been shown that both complexes bind specifically to human serum apotransferrin and the resulting adducts have been studied through spectroscopic and chromatographic techniques with the ultimate goal of preparing adducts with good selectivity for cancer cells due to the fact that tumour cells express high amounts of transferrin receptors on their cell surface.In order to investigate whether the cellular uptake of the complexes was mediated by apotransferrin or transferrin, we compared the antiproliferative efficacy of HInd[RuInd2Cl4] and HIm[RuIm2Cl4] with its apotransferrin- and transferrin-bound form in the human colon cancer cell line SW707 using the microculture tetrazolium test (MTT).Our results show that especially the transferrin-bound forms exhibit high antiproliferative activity, which exceeds that of the free complex, indicating that this protein can act as a carrier of the ruthenium complexes into the tumor cell.

Sign in / Sign up

Export Citation Format

Share Document