scholarly journals Screening of marine seaweeds for bioactive compound against fish pathogenic bacteria and active fraction analysed by gas chromatography– mass spectrometry

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Kingsley Erhons Enerijiofi ◽  
Funmilayo Hannah Akapo ◽  
Joseph Omorogiuwa Erhabor

Abstract Background Man has a long history of utilizing herbal preparations to treat infections. Therefore, this study aimed to investigate the quantitative phytochemical components, gas chromatography–mass spectrometry analysis, and the antibacterial properties of the aqueous and ethanol leaf extracts of Moringa oleifera on some clinical bacterial isolates. Results Aqueous and ethanol extractions from Moringa oleifera yielded 40.75% and 62.87%, respectively. Flavonoid (20.76 mg/100 g) was the highest, while saponin (2.00 mg/100 g) was the least of all phytochemicals detected. The proximate nutrient composition revealed that carbohydrate (46.59%) had the highest, while lipid (7.37%) was the least. Eleven compounds were detected in both extracts by gas chromatography–mass spectrometry. The eleven compounds identified had higher concentrations in the ethanol extract except 2-octenoic (26.09 mg/kg) acid and 1, 2-epoxyhexadecane (8.84 mg/kg) in aqueous extract which were considerably higher than 0.62 mg/kg and < 0.01 mg/kg in ethanol extract. The minimum inhibitory concentration and minimum bactericidal concentration were 6.25 mg/ml against the test organisms for ethanol extract. Conclusion The antibacterial activity of the ethanol extract was more active against the bacterial isolates than the aqueous, which increased as the extract concentration increases. The reports revealed that Moringa oleifera is an all-important herb that can inhibit infections from the studied pathogenic bacteria isolates.


HortScience ◽  
2021 ◽  
Vol 56 (10) ◽  
pp. 1195-1198
Author(s):  
Chunlian Jin ◽  
Dan Sun ◽  
Chang Wei ◽  
Zhenhua Guo ◽  
Chunmei Yang ◽  
...  

Gypsophila paniculata is an ornamental crop with medicinal value. To date, limited information has been reported about the natural products in G. paniculata to explain its medicinal function. The current study reports the natural products found in G. paniculata stem for the first time. Thirty-three compounds were isolated from the extract of G. paniculata stem and identified by gas chromatography-mass spectrometry, 10 of which have contents >2%. These were 2-O-methyl-D-mannopyranose (37.4706%), glycerol (12.5669%), two tetratetracontane isomer (7.6523 + 3.5145%), tetrahygro-4-pyranol (5.3254%), 1,6-anhydro-beta-d-glucopyranos (4.7507%), palmitic acid (4.1848%), 4-hydroxy-3-methoxystyrene (3.7439%), methyl-octadeca-9,12-dienoate (2.7490%), and 2-deoxy-D-galactose (2.6193%). Another bioactive compound, condrillasterol, was identified with 1.3384% content. We also reported that G. paniculata possesses antioxidant activity possibly associated with the presence of a phenolic chemical 4-hydroxy-3-methoxystyrene. Our data collectively demonstrate that G. paniculata contains some bioactive compounds with high contents and antioxidants, consistent with its role as a medicinal herb.


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