scholarly journals Antioxidant, α-Glucosidases and α-Amylase Inhibitory Activities of Persicaria odorata

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Kanya Thongra-ar ◽  
Piyanuch Rojsanga ◽  
Savita Chewchinda ◽  
Supachoke Mangmool ◽  
Pongtip Sithisarn
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Enzymes ◽  
Reactive Oxygen ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Oxygen Species ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Ic50 Values

The objects of this study were to determine the effects to reactive oxygen species and antioxidant enzymes levels in HEK-293 cells and inhibition of α-glucosidases and α-amylase enzymes of extracts from Persicaria odorata or phak phaeo. The ethanol extracts from the leaves and the stems of phak phaeo were investigated for their 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging activities (IC50 were 7.74 ± 0.47 and 7.91 ± 0.43 µg/mL, respectively). Cellular antioxidant effects in human embryonic kidney-293 (HEK-293) cells with these extracts (0.1 mg/mL) also increased the mRNA expressions of manganese superoxide dismutase (Mn-SOD), glutathione peroxidase 1 (GPx-1), catalase and glutathione reductase (GRe). The leaf extract showed the higher efficacies in the induction of the mRNA expressions of Mn-SOD, GPx-1 and GRe while the stem extract exhibited a stronger effect to the induction of catalase. Phak phaeo in vitro inhibitory effects to α-glucosidase enzyme (IC50 values of 9.82 ± 1.64 and 13.99 ± 1.45 µg/mL, respectively and also strong inhibition to α-amylase with IC50 values of 90.66 ± 8.75 and 19.96 ± 5.37 µg/mL, respectively). Lineweaver-Burk plot demonstrated that phak phaeo extracts inhibited α-glucosidase and α- amylase in non-competitive manners. Total phenolic and total flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride methods (the leaf and stem extracts were 22.89 ± 9.16 and 22.27 ± 8.77 g gallic acid equivalent in 100 g extract (g% GAE) and 7.20 ± 3.61 and 4.06 ± 1.73 g quercetin equivalent in 100 g extract (g% QE), respectively). Keywords: Antioxidant enzymes, DPPH, HEK-293, MTT assay, Persicaria odorata, Reactive oxygen species, Total phenolic, Total flavonoid, α-glucosidases, α-amylase

scholarly journals Time-Dependent Effects of Anesthetic Isoflurane on Reactive Oxygen Species Levels in HEK-293 Cells

2014 ◽  
Vol 4 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Yongxing Sun ◽  
Baiqi Cheng ◽  
Yuanlin Dong ◽  
Tianzuo Li ◽  
Zhongcong Xie ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Time Dependent ◽  
Oxygen Species ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals Phenolic Rich Fractions from Mycelium and Fruiting Body of Ganoderma lucidum Inhibit Bacterial Pathogens Mediated by Generation of Reactive Oxygen Species and Protein Leakage and Modulate Hypoxic Stress in HEK 293 Cell Line

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Jigni Mishra ◽  
Anivesh Joshi ◽  
Rakhee Rajput ◽  
Kaushlesh Singh ◽  
Anju Bansal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Fruiting Body ◽  
Ganoderma Lucidum ◽  
Chemical Constituents ◽  
Reactive Oxygen ◽  
Total Phenolic ◽  
Therapeutic Effects ◽  
Oxygen Species ◽  
Antioxidant Power ◽  
Hek 293

Ganoderma lucidum (G. lucidum) fungus (Family Ganodermataceae) is widely used as a traditional medicine in China, Japan, and many Asian countries on account of its numerous medicinal properties such as antioxidant, anticancer, antimicrobial, energy enhancing, and immunostimulatory. This broad spectrum of therapeutic effects exhibited by G. lucidum is ascribed to its abundance in several classes of chemical constituents, namely, carbohydrates, flavonoids, minerals, phenolics, proteins, and steroids which possess substantial bioactivities. The aim of the current study was to prepare phenolic rich fractions (PRFs) from aqueous extract of the Indian variety of G. lucidum mycelium and fruiting body. These fractions were assessed for their antioxidant capacity by TPC (total phenolic content), TFC (total flavonoid content), FRAP (ferric reducing antioxidant power), and ABTS [2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid] assays. Quantification of flavonoids and nucleobases present in the fractions was carried out by high-performance thin layer chromatography (HPTLC). The antibacterial activity of the fractions was evaluated against Escherichia coli, Salmonella typhi, and Staphylococcus aureus. The antibacterial mechanism of action of the PRFs was established to be generation of reactive oxygen species and leakage of proteins within bacterial cells. Additionally, the protective effect of the PRFs in counteracting hypoxia was observed in HEK 293 cell lines.

scholarly journals Influence of Acetylcholinesterase Inhibitors Used in Alzheimer’s Disease Treatment on the Activity of Antioxidant Enzymes and the Concentration of Glutathione in THP-1 Macrophages under Fluoride-Induced Oxidative Stress

2018 ◽  
Vol 16 (1) ◽  
pp. 10 ◽  
Author(s):  
Marta Goschorska ◽  
Izabela Gutowska ◽  
Irena Baranowska-Bosiacka ◽  
Katarzyna Piotrowska ◽  
Emilia Metryka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Reactive Oxygen Species ◽  
Alzheimer's Disease ◽  
Antioxidant Enzymes ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
Acetylcholinesterase Inhibitors ◽  
Oxygen Species ◽  
Ache Inhibitors

It has been reported that donepezil and rivastigmine, the acetylcholinesterase (AchE) inhibitors commonly used in the treatment of Alzheimer’s disease (AD), do not only inhibit AChE but also have antioxidant properties. As oxidative stress is involved in AD pathogenesis, in our study we attempted to examine the influence of donepezil and rivastigmine on the activity of antioxidant enzymes and glutathione concentration in macrophages—an important source of reactive oxygen species and crucial for oxidative stress progression. The macrophages were exposed to sodium fluoride induced oxidative stress. The antioxidant enzymes activity and concentration of glutathione were measured spectrophotometrically. The generation of reactive oxygen species was visualized by confocal microscopy. The results of our study showed that donepezil and rivastigmine had a stimulating effect on catalase activity. However, when exposed to fluoride-induced oxidative stress, the drugs reduced the activity of some antioxidant enzymes (Cat, SOD, GR). These observations suggest that the fluoride-induced oxidative stress may suppress the antioxidant action of AChE inhibitors. Our results may have significance in the clinical practice of treatment of AD and other dementia diseases.

Antioxidant enzymes and reactive oxygen species level of the Achromobacter xylosoxidans bacteria during hydrocarbons biotransformation

2018 ◽  
Vol 200 (7) ◽  
pp. 1057-1065 ◽  
Author(s):  
I. S. Sazykin ◽  
M. A. Sazykina ◽  
L. E. Khmelevtsova ◽  
E. Yu. Seliverstova ◽  
K. Sh. Karchava ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Enzymes ◽  
Reactive Oxygen ◽  
Species Level ◽  
Reactive Oxygen Species Level ◽  
Achromobacter Xylosoxidans ◽  
Oxygen Species
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