2A1-S05 Development of temperature-responsive cell culture surface by using PDMS substrate and characterization of the surface(Bio Assembler for 3D Cellular System Innovation)

Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

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Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface

Transplantation Journal ◽

10.1097/01.tp.0000110320.45678.30 ◽

2004 ◽

Vol 77 (3) ◽

pp. 379-385 ◽

Cited By ~ 420

Author(s):

Kohji Nishida ◽

Masayuki Yamato ◽

Yasutaka Hayashida ◽

Katsuhiko Watanabe ◽

Naoyuki Maeda ◽

...

Keyword(s):

Stem Cells ◽

Cell Culture ◽

Ex Vivo ◽

Corneal Epithelial ◽

Temperature Responsive ◽

Culture Surface ◽

Epithelial Sheet ◽

Responsive Cell

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Preparation of Poly(N-isopropylacrylamide) Grafted Polydimethylsiloxane by Using Electron Beam Irradiation

Journal of Robotics and Mechatronics ◽

10.20965/jrm.2013.p0631 ◽

2013 ◽

Vol 25 (4) ◽

pp. 631-636 ◽

Cited By ~ 4

Author(s):

Yoshikatsu Akiyama ◽

◽

Masayuki Yamato ◽

Teruo Okano

Keyword(s):

Cell Culture ◽

Electron Beam ◽

Cell Attachment ◽

Pdms Surface ◽

Chemical Treatments ◽

Temperature Responsive ◽

Treatment Conditions ◽

Culture Surface ◽

Surface Modified ◽

Responsive Cell

A poly(N-isopropylacrylamide) (PIPAAm) grafted poly(dimethylsiloxane) (PDMS) surface was prepared as a temperature-responsive cell culture surface by using electron beam (EB) irradiation. Different chemical treatments to modify the bare PDMS surface were investigated for subsequent grafting of PIPAAm, and treatment conditions were optimized to prepare the temperature-responsive cell culture surface. The PDMS surface was initially activated to form silanol groups with conventional O2 plasma or hydrochloric acid (HCl) treatment. Activated PDMS surfaces were individually immobilized with three different conventional silane compounds, i.e., 3-mercaptopropyltrimethoxysilane (MerTMS), 3-methacryloxypropyltrimethoxysilane (MetTMS), and 3-aminopropyltrimethoxysilane (AmiTMS). O2 plasma treatment made PDMS more hydrophilic. In contrast, PDMS surfaces activated with HCl treatment were relatively hydrophobic. Observation of the activated PDMS surface modified with MerTMS, MetTMS, and AmiTMS indicated that these silane compounds had been favorably immobilized on plasma-treated PDMS surfaces. FT-IR/ATR analysis demonstrated that immobilized silane compounds enabled PIPAAm grafting on the PDMS surface. Cell attachment and detachment analysis also suggested that the PDMS surface sequentially treated with O2 plasma and AmiTMS compound was a substrate appropriate for preparing a temperature-responsive cell culture surface by EB irradiation-induced PIPAAm grafting method. The intelligent surface may further be applied to mechanically stretchable temperature-responsive cell culture surfaces.

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