Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines

Caribbean sponges of the genus Smenospongia are a prolific source of chlorinated secondary metabolites. The use of molecular networking as a powerful dereplication tool revealed in the metabolome of S. aurea two new members of the smenamide family, namely smenamide F (1) and G (2). The structure of smenamide F (1) and G (2) was determined by spectroscopic analysis (NMR, MS, ECD). The relative and the absolute configuration at C-13, C-15, and C-16 was determined on the basis of the conformational rigidity of a 1,3-disubstituted alkyl chain system (i.e., the C-12/C-18 segment of compound (1). Smenamide F (1) and G (2) were shown to exert a selective moderate antiproliferative activity against cancer cell lines MCF-7 and MDA-MB-231, while being inactive against MG-63.

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In vitro cytotoxic potential of extracts from Aristolochia foetida Kunth against MCF-7 and bMECs cell lines

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.08.007 ◽

2021 ◽

Author(s):

Martín A. Lerma-Herrera ◽

Lidia Beiza-Granados ◽

Alejandra Ochoa-Zarzosa ◽

Joel E. López-Meza ◽

Juan D. Hernández-Hernández ◽

...

Keyword(s):

Cell Lines ◽

Cytotoxic Potential ◽

Mcf 7

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In vitro anticancer activity of microbial isolates from diverse habitats

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502011000200009 ◽

2011 ◽

Vol 47 (2) ◽

pp. 279-287 ◽

Cited By ~ 5

Author(s):

Angel Treasa Thomas ◽

Josyula Venkata Rao ◽

Volety Mallikarjuna Subrahmanyam ◽

Hariharapura Raghu Chandrashekhar ◽

Naseer Maliyakkal ◽

...

Keyword(s):

Cell Lines ◽

Biological Activities ◽

Bioactive Molecules ◽

Nuclear Staining ◽

Cytotoxic Potential ◽

Biochemical Tests ◽

Morphological Studies ◽

Aspergillus Sp ◽

Mcf 7

Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the cytotoxic potential. Nuclear staining and flow cytometric studies were carried out to assess the potential of the extracts in arresting the cell cycle. The crude ethylacetate extract of isolate F-21 showed promising results by MTT assay with IC50 as low as 20.37±0.36 µg/mL on HeLa, and 44.75±0.81 µg/mL on MCF-7 cells, comparable with Cisplatin. The isolate F-21 was identified as Aspergillus sp. Promising results were also obtained with B-2C and B-4E strains. Morphological studies, biochemical tests and preliminary chemical investigation of the extracts were also carried out.

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Cytotoxic potential of ethanol extract of Parquetina nigrescens on MCF-7, C4-2WT, HT 29 and HTC 116 cell lines

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp2018.4947 ◽

2018 ◽

Vol 12 (23) ◽

pp. 310-318

Author(s):

M. Onyegeme-Okerenta Blessing ◽

Agyare Christian ◽

D. Bradshaw Tracey ◽

A. Spriggs Keith

Keyword(s):

Cell Lines ◽

Ethanol Extract ◽

Cytotoxic Potential ◽

Ht 29 ◽

Mcf 7

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Ethiopian Medicinal Plants Traditionally Used for the Treatment of Cancer; Part 3: Selective Cytotoxic Activity of 22 Plants against Human Cancer Cell Lines

Molecules ◽

10.3390/molecules26123658 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3658

Author(s):

Solomon Tesfaye ◽

Hannah Braun ◽

Kaleab Asres ◽

Ephrem Engidawork ◽

Anteneh Belete ◽

...

Keyword(s):

Medicinal Plants ◽

Cell Lines ◽

Cancer Cell ◽

Mononuclear Cells ◽

Cancer Cell Lines ◽

Cytotoxic Activities ◽

Cell Lung Carcinoma ◽

Carcinoma Of The Esophagus ◽

Ic50 Values ◽

Mcf 7

Medicinal plants have been traditionally used to treat cancer in Ethiopia. However, very few studies have reported the in vitro anticancer activities of medicinal plants that are collected from different agro-ecological zones of Ethiopia. Hence, the main aim of this study was to screen the cytotoxic activities of 80% methanol extracts of 22 plants against human peripheral blood mononuclear cells (PBMCs), as well as human breast (MCF-7), lung (A427), bladder (RT-4), and cervical (SiSo) cancer cell lines. Active extracts were further screened against human large cell lung carcinoma (LCLC-103H), pancreatic cancer (DAN-G), ovarian cancer (A2780), and squamous cell carcinoma of the esophagus (KYSE-70) by using the crystal violet cell proliferation assay, while the vitality of the acute myeloid leukemia (HL-60) and histiocytic lymphoma (U-937) cell lines was monitored in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) microtiter assay. Euphorbia schimperiana, Acokanthera schimperi, Kniphofia foliosa, and Kalanchoe petitiana exhibited potent antiproliferative activity against A427, RT-4, MCF-7, and SiSo cell lines, with IC50 values ranging from 1.85 ± 0.44 to 17.8 ± 2.31µg/mL. Furthermore, these four extracts also showed potent antiproliferative activities against LCLC-103H, DAN-G, A2780, KYSE-70, HL-60, and U-937 cell lines, with IC50 values ranging from 0.086 to 27.06 ± 10.8 µg/mL. Hence, further studies focusing on bio-assay-guided isolation and structural elucidation of active cytotoxic compounds from these plants are warranted.

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Cytotoxic Potential of Industrial Hemp Extracts

Series II - Forestry • Wood Industry • Agricultural Food Engineering ◽

10.31926/but.fwiafe.2021.14.63.2.10 ◽

2021 ◽

Vol 14(63) (2) ◽

pp. 113-118

Author(s):

Alice GRIGORE ◽

◽

Lucia PIRVU ◽

Corina BUBUEANU ◽

Ioana ȚABREA ◽

...

Keyword(s):

Phenolic Compounds ◽

Secondary Metabolites ◽

Antitumor Activity ◽

Cell Lines ◽

Cancer Cell ◽

Cytotoxic Effect ◽

Cannabis Sativa ◽

Cytotoxic Potential ◽

Industrial Hemp ◽

Potential Antitumor

Industrial hemp (Cannabis sativa L.) is a source of fibers, oil and valuable secondary metabolites. Regarding phenolic compounds, it has to be noted that the plant biosynthesizes molecules with various pharmacological benefits. The aim of this study was to prove the cytotoxic potential of hemp polar and non-polar fractions against two cancer cell lines (BT-20 and U87). The study revealed the potential antitumor activity of industrial hemp selective fractions but a correlation between polyphenols content and the cytotoxic effect could not be established.

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Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines

Journal of Mathematical and Fundamental Sciences ◽

10.5614/j.math.fund.sci.2019.51.3.2 ◽

2019 ◽

Vol 51 (3) ◽

pp. 225-242

Author(s):

Meiridha Mutiara Andania ◽

◽

Friardi Ismed ◽

Muhammad Taher ◽

Solachuddin Jauhari Arief Ichwan ◽

...

Keyword(s):

Medicinal Plants ◽

Cell Lines ◽

Cytotoxic Activities ◽

Mcf 7

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Fast Detection of Two Smenamide Family-Members Using Molecular Networking

10.20944/preprints201910.0091.v1 ◽

2019 ◽

Author(s):

Alessia Caso ◽

Germana Esposito ◽

Gerardo Della Sala ◽

Joseph R. Pawlik ◽

Roberta Teta ◽

...

Keyword(s):

Secondary Metabolites ◽

Cell Lines ◽

Absolute Configuration ◽

Antiproliferative Activity ◽

Spectroscopic Analysis ◽

Alkyl Chain ◽

Molecular Networking ◽

New Members ◽

Fast Detection ◽

Mcf 7

Caribbean sponges of the genus Smenospongia are a prolific source of chlorinated secondary metabolites. The use of molecular networking as a powerful dereplication tool revealed the presence in the metabolome of S. aurea of two new members of the smenamide family, namely smenamide F (1) and G (2). The structure of smenamide F (1) and G (2) was determined by spectroscopic analysis (NMR, MS, ECD). The relative and the absolute configuration at C-13, C-15, and C-16 was determined on the basis of the conformational rigidity of a 1,3-disubstituted alkyl chain system (i.e. the C-12/C-18 segment of compound 1). Smenamide F (1) and G (2) were shown to exert a selective moderate antiproliferative activity against cancer cell lines MCF-7 and MDA-MB-231, while being inactive against MG-63.

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Synthesis and In Vitro Cytotoxic Activity of Chromenopyridones

International Journal of Medicinal Chemistry ◽

10.1155/2013/984329 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Balwinder Singh ◽

Vishal Sharma ◽

Gagandeep Singh ◽

Rakesh Kumar ◽

Saroj Arora ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Hep G2 ◽

Cytotoxic Potential ◽

Human Cancer Cell Lines ◽

Mcf 7

Novel substituted chromenopyridones (3a–j and 6a–d) were synthesized and evaluated in vitro for the cytotoxic activity against various human cancer cell lines such as prostate (PC-3), breast (MCF-7), CNS (IMR-32), cervix (Hela), and liver (Hep-G2). preliminary cytotoxic screening showed that all the compounds possess a good to moderate inhibitory activity against various cancer cell lines. Particularly, compound 6b bearing allyl moiety displayed a significant cytotoxic potential in comparison to standard drugs.

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