scholarly journals Fast Detection of Two Smenamide Family Members Using Molecular Networking

Marine Drugs ◽  
2019 ◽  
Vol 17 (11) ◽  
pp. 618 ◽  
Author(s):  
Alessia Caso ◽  
Germana Esposito ◽  
Gerardo Della Sala ◽  
Joseph R. Pawlik ◽  
Roberta Teta ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cell Lines ◽  
Absolute Configuration ◽  
Antiproliferative Activity ◽  
Spectroscopic Analysis ◽  
Alkyl Chain ◽  
Molecular Networking ◽  
New Members ◽  
Fast Detection ◽  
Mcf 7

Caribbean sponges of the genus Smenospongia are a prolific source of chlorinated secondary metabolites. The use of molecular networking as a powerful dereplication tool revealed in the metabolome of S. aurea two new members of the smenamide family, namely smenamide F (1) and G (2). The structure of smenamide F (1) and G (2) was determined by spectroscopic analysis (NMR, MS, ECD). The relative and the absolute configuration at C-13, C-15, and C-16 was determined on the basis of the conformational rigidity of a 1,3-disubstituted alkyl chain system (i.e., the C-12/C-18 segment of compound (1). Smenamide F (1) and G (2) were shown to exert a selective moderate antiproliferative activity against cancer cell lines MCF-7 and MDA-MB-231, while being inactive against MG-63.

scholarly journals Fast Detection of Two Smenamide Family-Members Using Molecular Networking

2019 ◽  
Author(s):  
Alessia Caso ◽  
Germana Esposito ◽  
Gerardo Della Sala ◽  
Joseph R. Pawlik ◽  
Roberta Teta ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cell Lines ◽  
Absolute Configuration ◽  
Antiproliferative Activity ◽  
Spectroscopic Analysis ◽  
Alkyl Chain ◽  
Molecular Networking ◽  
New Members ◽  
Fast Detection ◽  
Mcf 7

Caribbean sponges of the genus Smenospongia are a prolific source of chlorinated secondary metabolites. The use of molecular networking as a powerful dereplication tool revealed the presence in the metabolome of S. aurea of two new members of the smenamide family, namely smenamide F (1) and G (2). The structure of smenamide F (1) and G (2) was determined by spectroscopic analysis (NMR, MS, ECD). The relative and the absolute configuration at C-13, C-15, and C-16 was determined on the basis of the conformational rigidity of a 1,3-disubstituted alkyl chain system (i.e. the C-12/C-18 segment of compound 1). Smenamide F (1) and G (2) were shown to exert a selective moderate antiproliferative activity against cancer cell lines MCF-7 and MDA-MB-231, while being inactive against MG-63.

scholarly journals Synthesis of new 3-(substituted-phenyl)-N-(2-hydroxy-2-(substituted-phenyl)ethyl)-N-methylthiophene-2-sulfonamide derivatives as antiproliferative agents

2018 ◽  
Vol 9 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Chandrakant Pawar ◽  
Dattatraya Pansare ◽  
Devanand Shinde
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
1H Nmr ◽  
13C Nmr ◽  
Antiproliferative Activity ◽  
Positive Control ◽  
Antiproliferative Agents ◽  
Ic50 Values ◽  
Mcf 7

In the present work, we report the synthesis of a series of 3-(substituted phenyl)-N-(2-hydroxy-2-(substituted-phenyl)ethyl)-N-methylthiophene-2-sulfonamide derivatives through Suzuki and Buchwald reaction. We have optimized methodology for targets from milligram to multi-gram scale. The newly synthesized compounds were characterized by 1H NMR, 19F NMR, 13C NMR, LC-MS techniques and purity was further checked by HPLC. The compounds were evaluated for their in-vitro antiproliferative activity against MCF-7, HeLa, A-549 and Du-145 cancer cell lines by CCK-8 assay. The preliminary bioassay suggests that most of the compounds show antiproliferation with different degrees and 5-fluorouracil was used as positive control. Among these compounds 2d, 2g, 2i, 4e, 4h and 4k are most active compared to the standard. All the synthesized compounds show IC50 values from 1.82-9.52 µM in different cell lines. Amongst these, compounds 2d, 2g, 2i, 4e, 4h and 4k were most potent, with IC50 values ranging from 1.82-4.28 µM in different cell lines.

scholarly journals Corrigendum to: Screening of Ipomoea tuba Leaf Extract for Identification of Bioactive Compounds and Evaluation of Its in vitro Antiproliferative Activity Against MCF-7 and HeLa Cells (Published: Food Technol. Biotechnol. 58 (1) 71-75 (2020) https://doi.org/10.17113/ftb.58.01.20.6351)

2020 ◽  
Vol 58 (3) ◽  
pp. 356-356
Author(s):  
Thirupati Chinna Venkateswarulu ◽  
Gaddam Eswaraiah ◽  
Srirrama Krupanidhi ◽  
Karlapudi Abraham Peele ◽  
Indira Mikkili ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bioactive Compounds ◽  
Hela Cells ◽  
Antiproliferative Activity ◽  
Leaf Extract ◽  
Mcf 7

The authors requested the replacement of Fig. 1. Morphology of MCF-7 cells after the treatment with the extract of Ipomoea tuba leaf: a) untreated MCF-7 cell lines, and b-g) treated with different concentrations (5, 10, 25, 50, 75 and 100 μg/mL respectively) of the leaf extract The change includes the replacemend of images 1c-1g.

scholarly journals ANTIPROLIFERATIVE ACTIVITY OF ELEPHANTOPUS SCABER MEDIATED SILVER NANOPARTICLES AGAINST MCF-7, A-549, SCC-40, AND COLO-205 HUMAN CANCER CELL LINES

2019 ◽  
pp. 163-167
Author(s):  
ASHWINI S SHINDE ◽  
VIJAY D MENDHULKAR
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Cancer Cell Lines ◽  
Synergistic Activity ◽  
Standard Drug ◽  
Elephantopus Scaber ◽  
The Impact ◽  
Mcf 7

Objective: To unearth the applications of nanotechnology in medicine has become imperative with all the advancements in the technique. In the current study, we have attempted to exploit the anticancer ability of the green synthesized silver nanoparticles (AgNPs). Methods: The AgNPs were synthesized using 60% methanol (H-MeOH) Elephantopus scaber leaf extract, characterized, and discussed priorly. The effect of AgNPs was studied on the human breast (MCF-7), lung (A-549), oral (SCC-40), and colon (COLO-205) cancer cell lines through sulforhodamine B assay. We also carried out the synergistic activity with standard drug adriamycin (ADR). Results: According to the results obtained, AgNPs showed good antiproliferative activity with GI50 <10 μg/ml on MCF-7, A-549, and SCC-40 cell lines when compared with the standard drug ADR. However, for COLO-205 cell line, the impact was 17.4 μg/ml and thus the treatment was less effective. Conclusion: The synergistic effect of AgNPs+ADR was even better for all the four cell lines than that of the AgNPs alone.

Synthesis, structural characterization and antiproliferative activity on MCF-7 and A549 tumor cell lines of [Cu(N-N)(β3-aminoacidate)]NO3 complexes (Casiopeínas®)

2020 ◽  
Vol 506 ◽  
pp. 119542 ◽  
Author(s):  
Jonathan Román Valdez-Camacho ◽  
Yaquelinne Pérez-Salgado ◽  
Adrián Espinoza-Guillén ◽  
Virginia Gómez-Vidales ◽  
Carlos Alberto Tavira-Montalvan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Structural Characterization ◽  
Antiproliferative Activity ◽  
Tumor Cell Lines ◽  
Mcf 7

Synthesis and Reactivity of 6,8-Dibromo-2-ethyl-4H-benzo[d][1,3]oxazin-4-one Towards Nucleophiles and Electrophiles and Their Anticancer Activity

2019 ◽  
Vol 19 (4) ◽  
pp. 538-545 ◽  
Author(s):  
Maher A. El-hashash ◽  
Amira T. Ali ◽  
Rasha A. Hussein ◽  
Wael M. El-Sayed
Keyword(s):  
Anticancer Activity ◽  
Cancer Cells ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Repair Mechanisms ◽  
Breast Cells ◽  
Nitrogen Nucleophiles ◽  
Quinazolinone Derivatives ◽  
Benzaldehyde Derivatives ◽  
Mcf 7

Background: The genetic heterogeneity of tumor cells and the development of therapy-resistant cancer cells in addition to the high cost necessitate the continuous development of novel targeted therapies. Methods: In this regard, 14 novel benzoxazinone derivatives were synthesized and examined for anticancer activity against two human epithelial cancer cell lines; breast MCF-7 and liver HepG2 cells. 6,8-Dibromo-2- ethyl-4H-benzo[d][1,3]oxazin-4-one was subjected to react with nitrogen nucleophiles to afford quinazolinone derivatives and other related moieties (3-12). Benzoxazinone 2 responds to attack with oxygen nucleophile such as ethanol to give ethyl benzoate derivative 13. The reaction of benzoxazinone 2 with carbon electrophile such as benzaldehyde derivatives afforded benzoxazinone derivatives 14a and 14b.The structure of the prepared compounds was confirmed with spectroscopic tools including IR, 1H-NMR, and 13C-NMR. Results: Derivatives 3, 9, 12, 13, and 14b exhibited high antiproliferative activity and were selective against cancer cells showing no toxicity in normal fibroblasts. Derivative 3 with NH-CO group in quinazolinone ring was effective only against breast cells, while derivative 12 with NH-CO group in imidazole moiety was only effective against liver cells probably through arresting cell cycle and enabling repair mechanisms. The other derivatives (9, 13, and 14b) had broader antiproliferative activity against both cell lines. These derivatives enhance the expression of the p53 and caspases 9 and 3 to varying degrees in both cell lines. Derivative 14b caused the highest induction in the investigated genes and was the only derivative to inhibit the EGFR activity. Conclusions: The unique features about derivative 14b could be attributed to its high lipophilicity, high carbon content, or its extended conjugation through planar aromatic system. More investigations are required to identify the lead compound(s) in animal models.

Antiproliferative activity of lignans against the breast carcinoma cell lines MCF 7 and BT 20

2011 ◽  
Vol 285 (4) ◽  
pp. 1145-1151 ◽  
Author(s):  
Sibylle Abarzua ◽  
Tatsuo Serikawa ◽  
Marlen Szewczyk ◽  
Dagmar-Ulrike Richter ◽  
Birgit Piechulla ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Breast Carcinoma Cell Lines ◽  
Mcf 7

scholarly journals A New Tocopherol Derivative and Cytotoxicity from the Leaves of Dalbergia velutina

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Sutin Kaennakam ◽  
Thammarat Aree ◽  
Kitiya Rassamee ◽  
Pongpun Siripong ◽  
Santi Tip-pyang
Keyword(s):  
Nmr Spectroscopy ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spectroscopic Analysis ◽  
Cancer Cell Lines ◽  
The Absolute ◽  
Ht 29 ◽  
Mcf 7

A new tocopherol derivative, named (+)-α-tocuspirone (1), along with eleven known compounds, including six tocopherol derivatives (2–7) and five triterpenes (8–12) were isolated from the leaves of Dalbergia velutina. Their structures were determined by spectroscopic analysis especially NMR spectroscopy. The absolute configurations of 1 and 2 were assigned by NOESY experiments and ECD calculations. All isolated compounds were evaluated for their cytotoxicity against five cancer cell lines (KB, HeLa S-3, HT-29, MCF-7 and HepG-2). Dioslupecin A (10) showed potent cytotoxicity against all the five cancer cell lines with IC50 values in the range of 0.28–2.05 μM. In addition, caffeoxylupeol (12) showed potent cytotoxicity against KB cell with an IC50 value of 2.28 μM.

scholarly journals Antiproliferative activity of natural coumarins from Calophyllum incrasaptum M.R Henderson-Wyatt Smith against human breast cancer cells MCF

2018 ◽  
Vol 154 ◽  
pp. 04003
Author(s):  
Jamilah Abbas ◽  
Linar Z Udin ◽  
Muhammad Hanafi
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Alamar Blue ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

Objective: to evaluated the antiproliferative activity of natural coumarin from Calophyllum incrasaptum M.R Henderson-Wytt Smith against human breast cancer cells MCF-7. Methode : Coumarin from ethyl acetate fraction of C. incrasaptum M.R Henderson-Wyatt Smith was isolated by coloumn chromatographyic and structure elucidated by using spectroscopic methods and isolate compound was evaluated for their antiproliferative activities in the alamar blue assay. Result: Coumarin have antiproliferative activity against MCF-7 cancer cell lines through alamar blue assay for 4 h after treatment. Conclusions: coumarin showed good activity against cancer cell lines with IC50 value of 2.23 μg/mL.

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