Protective Effect of Spirulina against Cell DNA Damage and Oxidative Stress Induced by Exhaustive Exercise

The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues (p<0.01) and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system.

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Evaluation of DNA Damage and Oxidative Stress in Road Pavement Workers Occupationally Exposed to Polycyclic Aromatic Hydrocarbons

International Conference on Advances in Agricultural, Biological & Environmental Sciences (AABES-2014) Oct 15-16, 2014 Dubai (UAE) ◽

10.15242/iicbe.c1014023 ◽

2014 ◽

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Road Pavement ◽

Occupationally Exposed ◽

Polycyclic Aromatic ◽

And Oxidative Stress

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Antia, a Natural Antioxidant Product, Attenuates Cognitive Dysfunction in Streptozotocin-Induced Mouse Model of Sporadic Alzheimer’s Disease by Targeting the Amyloidogenic, Inflammatory, Autophagy, and Oxidative Stress Pathways

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/4386562 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Nesrine S. El Sayed ◽

Mamdooh H. Ghoneum

Keyword(s):

Oxidative Stress ◽

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Neurodegenerative Diseases ◽

Protective Effect ◽

Cognitive Dysfunction ◽

Protective Role ◽

Sporadic Alzheimer’S Disease ◽

Stat3 Pathway ◽

And Oxidative Stress

Background. Many neurodegenerative diseases such as Alzheimer’s disease are associated with oxidative stress. Therefore, antioxidant therapy has been suggested for the prevention and treatment of neurodegenerative diseases. Objective. We investigated the ability of the antioxidant Antia to exert a protective effect against sporadic Alzheimer’s disease (SAD) induced in mice. Antia is a natural product that is extracted from the edible yamabushitake mushroom, the gotsukora and kothala himbutu plants, diosgenin (an extract from wild yam tubers), and amla (Indian gooseberry) after treatment with MRN-100. Methods. Single intracerebroventricular (ICV) injection of streptozotocin (STZ) (3 mg/kg) was used for induction of SAD in mice. Antia was injected intraperitoneally (i.p.) in 3 doses (25, 50, and 100 mg/kg/day) for 21 days. Neurobehavioral tests were conducted within 24 h after the last day of injection. Afterwards, mice were sacrificed and their hippocampi were rapidly excised, weighed, and homogenized to be used for measuring biochemical parameters. Results. Treatment with Antia significantly improved mice performance in the Morris water maze. In addition, biochemical analysis showed that Antia exerted a protective effect for several compounds, including GSH, MDA, NF-κB, IL-6, TNF-α, and amyloid β. Further studies with western blot showed the protective effect of Antia for the JAK2/STAT3 pathway. Conclusions. Antia exerts a significant protection against cognitive dysfunction induced by ICV-STZ injection. This effect is achieved through targeting of the amyloidogenic, inflammatory, and oxidative stress pathways. The JAK2/STAT3 pathway plays a protective role for neuroinflammatory and neurodegenerative diseases such as SAD.

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Styrax camporum, a typical species of the Brazilian cerrado, attenuates DNA damage, preneoplastic lesions and oxidative stress in experimental rat colon carcinogenesis

Journal of Toxicology and Environmental Health Part A ◽

10.1080/15287394.2021.1910090 ◽

2021 ◽

pp. 1-11

Author(s):

Pollyanna Francielli De Oliveira ◽

Luis Fernando Leandro ◽

Ricardo Andrade Furtado ◽

Natália Helen Ferreira ◽

Patrícia Mendonça Pauletti ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Colon Carcinogenesis ◽

Rat Colon ◽

Brazilian Cerrado ◽

Preneoplastic Lesions ◽

Typical Species ◽

And Oxidative Stress

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Haloperidol-loaded lipid-core polymeric nanocapsules reduce DNA damage in blood and oxidative stress in liver and kidneys of rats

Journal of Nanoparticle Research ◽

10.1007/s11051-015-2979-4 ◽

2015 ◽

Vol 17 (4) ◽

Cited By ~ 3

Author(s):

Katiane Roversi ◽

Dalila M. Benvegnú ◽

Karine Roversi ◽

Fabíola Trevizol ◽

Luciana T. Vey ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Lipid Core ◽

Polymeric Nanocapsules ◽

And Oxidative Stress

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O XVII B.2 Determination of endogenous DNA damage, oxidated DNA bases and oxidative stress in primary human colon cells and modulation by plant ingredients

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/s0027-5107(97)83211-3 ◽

1997 ◽

Vol 379 (1) ◽

pp. S171

Author(s):

Beatrice L. Pool-Zobel ◽

Salomon L. Abrahamse ◽

Daniela Oberreuther ◽

Sylvia Treptow-van Lishaut ◽

Gerhard Rechkemmer

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Human Colon ◽

Dna Bases ◽

Colon Cells ◽

And Oxidative Stress

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Reduction of doxorubicin-induced cytotoxicity and mitochondrial damage by betanin in rat isolated cardiomyocytes and mitochondria

Human & Experimental Toxicology ◽

10.1177/09603271211022800 ◽

2021 ◽

pp. 096032712110228

Author(s):

AA Hafez ◽

Z Jamali ◽

S Samiei ◽

S Khezri ◽

A Salimi

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Bioactive Compound ◽

Isolated Cardiomyocytes ◽

Cardiac Inflammation ◽

Flow Cytometric ◽

Significant Toxicity ◽

Ros Formation ◽

Gene Regulatory ◽

And Oxidative Stress

Doxorubicin (DOX) is an anticancer drug which is used for treatment of several types of cancers. But the clinical use of doxorubicin is limited because of its cardiotoxicity and cardiomyopathy. Mitochondrial-dependent oxidative stress and cardiac inflammation appear to be involved in doxorubicin-induced cardiotoxicity. Betanin as a bioactive compound in Beetroot ( Beta vulgaris L.) displays anti-radical, antioxidant gene regulatory and cardioprotective activities. In this current study, we investigated the protective effect of betanin on doxorubicin-induced cytotoxicity and mitochondrial-dependent oxidative stress in isolated cardiomyocytes and mitochondria. Isolated cardiomyocytes and mitochondria were treated with three concentrations of betanin (1, 5 and 10 µM) and doxorubicin (3.5 µM) for 6 h. The parameters of cellular and mitochondrial toxicity were analyzed using biochemical and flow cytometric methods. Our results showed a significant toxicity in isolated cardiomyocytes and mitochondria in presence of doxorubicin which was related to reactive oxygen species (ROS) formation, increase in malondialdehyde (MDA), increase in oxidation of GSH to GSSG, lysosomal/mitochondrial damages and mitochondrial swelling. While betanin pretreatment reverted doxorubicin-induced cytotoxicity and oxidative stress in isolated cardiomyocytes and mitochondria. These results suggest that betanin elicited a typical protective effect on doxorubicin-induced cytotoxicity and oxidative stress. It is possible that betanin could be used as a useful adjuvant in combination with doxorubicin chemotherapy for reduction of cardiotoxicity and cardiomyopathy.

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