scholarly journals Composition of Volatile Oil and Methanolic Extract of Jordanian Melissa Officinals L. and Actions Againsthuman Cancer Cell Lines

2016 ◽  
Vol 32 (5) ◽  
pp. 2355-2362 ◽  
Author(s):  
Salem Barakat ◽  
M. Hudaib ◽  
Noor EL-asadand ◽  
D. Burns
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Methanolic Extract ◽  
Volatile Oil ◽  
Cancer Cell Lines

scholarly journals Decorosides A and B, Cytotoxic Flavonoid Glycosides from the Leaves of Rhododendron decorum

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Mostafa E. Rateb ◽  
Hossam M. Hassan ◽  
El-Shaimaa A. Arafa ◽  
Marcel Jaspars ◽  
Rainer Ebel
Keyword(s):  
Spectral Data ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Methanolic Extract ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
Flavonoid Glycosides ◽  
Cytotoxic Activities ◽  
Diphenyltetrazolium Bromide

Bioassay and NMR-guided fractionation of the methanolic extract of Rhododendron decorum leaves resulted in the isolation of two new flavonoid glycosides, 5,7-dihydroxy-6,8-dimethyldihydroflavanone-7-O-α-L-arabinopyranosyl(l→6)-β-D-glucopyranoside (decoroside A, 1) and its 3-hydroxy congener (decoroside B, 2), along with five known compounds myricitrin (3), afzelin (4), (-)-epicatechin (5), (+)-catechin (6), and ampeloptin (7). The structures of the isolated compounds were elucidated by extensive interpretation of their spectral data. Biological evaluation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed promising cytotoxic activities of these compounds against different cancer cell lines.

scholarly journals Phytochemistry, Antioxidant Activity, Antiproliferative Effect, and Acute Toxicity Testing of Two Moroccan Aristolochia Species

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Mohammed Bourhia ◽  
Fatima Ezzahra Laasri ◽  
Samir Iben Moussa ◽  
Riaz Ullah ◽  
Ahmed Bari ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanolic Extract ◽  
Cancer Cell Lines ◽  
Antiproliferative Effect ◽  
Antiproliferative Effects ◽  
Phytochemical Composition ◽  
Ht 29

Ethnopharmacological Relevance. Aristolochia baetica (A. baetica) and Aristolochia paucinervis (A. paucinervis) have been largely used in Moroccan folk medicine. The objective of the study was to investigate the phytochemical composition, the antioxidant activity, the antiproliferative effect, and the acute toxicity of the methanolic extract of A. baetica and A. paucinervis roots. Materials and Methods. Phytochemical composition of the methanolic extract of A. baetica and A. paucinervis roots were studied using qualitative and quantitative methods, the antioxidant activity was evaluated using DPPH assay, the antiproliferative effects against human cancer cell lines (T-24, HT-29, and Hep G-2) was assessed using WST1 assay, and the acute toxicity was carried out orally by gavage of single dose 2000 mg/kg to mice for 14 days. Results. The two studied plants have different classes of secondary metabolites. The concentrations of the total polyphenolic content of A. baetica and A. paucinervis root extracts were estimated at 360 ± 20 mg GAE/g and 280 ± 27 mg GAE/g, respectively. The total flavonoids content of A. baetica and A. paucinervis extracts were estimated at 35 ± 8 mg QE/g and 235 ± 7 mg QE/g, respectively. A. baetica and A. paucinervis extracts exhibited promising DPPH activity with IC50 values of 150 ± 8 μg/ml and 160 ± 10 μg/ml, respectively. The extracts exerted also antiproliferative effects on all tested cancer cell lines (T-24, HT-29, and Hep G-2) with IC50 values ranging from 6 ± 1 μg/ml to 380 ± 7 μg/ml. Regarding the results of acute toxicity study, no signs of toxicities nor mortalities were observed on the oral treated mice with 2000 mg/kg of the two investigated exacts. Conclusion. The methanolic extracts of A. baetica and A. paucinervis possess several phytochemicals that exhibited promising free radical scavenging activity and antiproliferative effects.

scholarly journals Cytotoxic Effect of Jati Belanda Leaves towards Cancer Cell Lines

2017 ◽  
Vol 6 (2) ◽  
pp. 35
Author(s):  
Muhammad Da'i
Keyword(s):  
Molecular Mechanism ◽  
Cell Lines ◽  
Cancer Cell ◽  
Volatile Oil ◽  
Cancer Cell Lines ◽  
Cancer Cell Growth ◽  
Breast Cancer Cell Growth ◽  
Mtt Method ◽  
Ic50 Value

The initial research of Jati Belanda leaves extract (JBE) showed the inhibition of breast cancer cell growth (T47D). The phytochemistry screening showed that JBE contain flavonoid, alkaloid, polifenol, and volatile oil. The development of anticancer drugs need the molecular mechanism investigation in order to produce cancer-targeted drugs. The objective of this research is to determine the molecular mechanism of JBE cytotoxicity effect towards cancer cell lines. This research began with cytotoxicity asssay in vitro of JBE towards some cancer cell lines by MTT method. JBE was given in some variety of concentration. The result of this study showed that JBE do not contain tirosid, and contain flavonoid in the concentration of 0,976%. The result of cytotoxicity assay towards MCF-7, HeLa, T47D and Vero showed IC50 value 36,50; 58,02; 53,36; 1806,22 dan 2451,65 µg/mL respectively. It is concluded that JBE have a strong potency to inhibit the growth of WiDr cancer cell line.Keywords : jati belanda, T47D cells, cytotoxicity

scholarly journals The effect of crude methanolic extract of ginger (Zingibe officinale) root in different cell lines in vitro

2009 ◽  
Vol 3 (1) ◽  
pp. 122-129
Author(s):  
Shamaa Ismael Kadhum ◽  
Safaa Al-deen Ahmed Alqysi
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Plant Extract ◽  
Normal Cell ◽  
Methanolic Extract ◽  
Cancer Cell Lines ◽  
The Other ◽  
Normal Cell Line

This study involved the affectivity of crude methanolic extract of ginger root on different cells line in vitro, four cancer cell lines were tested Hela, L20B,Hep2, AMN3 compared with normal cell line (REF)and transformed cell line (Vero). Four extract concentrations were prepared (125,250,500,1000) µg/ml respectively, the results showed a significant inhibitory effect on the growth of different cell lines under study, also regression showed a significant negative relationship between plant extract and cell lines,1000 µg/ml concentration showed significant effect on cell lines growth (HELA,Hep2,L20B and Vero) on the other hand AMN3 was not affected by the plant extract, there was a direct relationship between concentrations and the rate of inhibition of the cell lines, on the other hand the normal cell line were more effected than cancer cell lines under study.

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