scholarly journals Exploration, phenolic content determination, and antioxidant activity of dominant pteridophytes in Gunung Malang Village, Mount Halimun Salak National Park, Indonesia

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
RINDITA RINDITA ◽  
Vivi Anggia ◽  
EKA RAHMAESA ◽  
RETNA KUSUMA DEVI ◽  
LIDIA FATMAH ALAWIYAH
Keyword(s):  
Antioxidant Activity ◽  
National Park ◽  
Phenolic Content ◽  
Abiotic Factors ◽  
Ultrasonic Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Ecological Requirements ◽  
Content Determination ◽  
Closed Canopy

Abstract. Rindita, Anggia V, Rahmaesa E, Devi RK, Alawiyah LF. 2020. Exploration, phenolic content determination, and antioxidant activity of dominant pteridophytes in Gunung Malang Village, Mount Halimun Salak National Park, Indonesia. Biodiversitas 21: 3676-3682. Pteridophytes have been known to have pharmacological activities as an antioxidant, anti-inflammation, antimicrobials, and anticancer, such as Selaginella sp. In this research, exploration was conducted in Gunung Malang Village, Mount Halimun Salak National Park (TNGHS), West Java. Samples were collected by purposive sampling method, related to its abundant populations, Histiopteris incisa, Nephrolepis biserrata, and Selaginella willdenowii were collected based on different height and light intensity. Abiotic factors were measured to know their ecological requirements. Samples were extracted using ultrasonic method. Organoleptic test, yield, and phytochemical screening were done for the crudes extract. Total phenolic level was determined with Folin-Ciocalteu method and antioxidant activity test with DPPH. Phytochemical screening showed that H. incisa contains phenolics, saponins, tannins, and steroids; N. biserrata contains phenolics, flavonoids, and saponins while S. willdenowii contains phenolics, alkaloids, flavonoids, saponins, and tannins. Phenolic content of H. incisa and N. biserrata from 700 m. asl.was 9.8523 mg GAE/g ± 0.9694 and 17.5399 mg GAE/g ± 0.5350 respectively, from 1000 m. asl. was 18.1231 mgGAE/g ±2.1535 and 8.8468 mg GAE/g ± 0.1437. S. willdenowii extract collected from shaded canopy forest was 19.2324 mg GAE/g ± 0.6041 and opened canopy forest was 38.7087 mg GAE/g ± 1.484. Antioxidant IC50 of H. incisa and N. biserrata from 700 m. asl. respectively was 96.4271 ppm and 85.1907 ppm, while from 1000 m. asl. was 75.6381 ppm and 95.0678 ppm, S. willdenowii from the closed canopy is 101.7326 ppm, while from the opened canopy was 92.0998 ppm. This study shows that all ferns tested gave significant antioxidant activity and was influenced by environmental factors.

A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

2021 ◽  
Vol 3 (2) ◽  
pp. 43
Author(s):  
Awalul Fatiqin ◽  
Hanif Amrulloh ◽  
Ike Apriani ◽  
Aneke Lestari ◽  
Berta Erawanti ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Leaf Part ◽  
Different Parts

In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.

scholarly journals Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan

2019 ◽  
Vol 5 (1) ◽  
pp. 33-42
Author(s):  
Syamsu Nur ◽  
Fitriyanti Jumaetri Sami ◽  
Akbar Awaluddin ◽  
Mutiara Indah Ayu Afsari
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Gmelina Arborea ◽  
Flavonoid Content ◽  
Coefficient Corrélation ◽  
Phenolic And Flavonoid

This experiment aims to determine the correlation of total phenolic and flavonoid content of jati putih leaves fraction (Gmelina arborea Roxb.) towards Antioxidant activity .  Sample was extracted by maceration method using ethanol 70% to obtain the ethanol extract (EE), followed by liquid-liquid extraction method to obtain fraction of ethyl acetate (EA) and n-Hexane (EH). The phytochemical screening  and determination of total phenolic and flavonoid content were done by colorimetric method. Antioxidant activity were done by DPPH, FRAP and ABTS methods. Phytochemical screening showed positive results for flavonoids, phenolic and saponins.  The largest total phenolic content was found on EA (11,59 µg/ml ± 0,3 %b/b EAG) and the largest total flavonoid content was on EA (3,88 µg/ml ± 0,02 %b/b EK). The total phenolic and flavonoid content of Jati putih leaves has a correlation with antioxidant activity. The coefficient correlation of activity on reducingDPPH radical was 56,7% (total of phenolic content) and 57,8% (total of flavonoid content) and on iron reduction power in FRAP method  was 99,9% (total of phenolics and flavonoids content). The relationship with the activity in reducing radical ABTS obtained coefficient correlation of 57,0% and 58,1% for total phenolic and flavonoids contents, respectively.

scholarly journals PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC CONTENT, AND ANTIOXIDANT ACTIVITY OF WATER, ETHYL ACETATE, AND N-HEXANE FRACTIONS FROM MISTLETOE MORINGA OLEIFERA LAM. (DENDROPHTHOE PENTANDRA (L.) MIQ.)

2018 ◽  
Vol 11 (10) ◽  
pp. 104
Author(s):  
Nia Kristiningrum ◽  
Lestyo Wulandari ◽  
Aini Zuhriyah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Significance Level ◽  
Water Fraction

Objective: This study was to investigate the phytochemical screening, total phenolic content (TPC), and antioxidant activity of fractions of water, ethyl acetate, and n-hexane from Dendrophthoe pentandra (L.) Miq. and mistletoe of Moringa oleifera Lam.Methods: Simplicia extracted with 96% ethanol using maceration method. Liquid-liquid extraction was used for fractionation with water, ethyl acetate, and n-hexane as solvent. Phytochemical screening, determination of TPC, and antioxidant activity were performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method. The TPC was measured by Folin–Ciocalteu reagent and use gallic acid (GA) as a standard.Results: Water fraction contains saponin and polyphenols. Ethyl acetate fraction contains alkaloids, flavonoids, saponins, terpenoids, and polyphenols. N-hexane fraction only contains alkaloids and terpenoids. The TPC and antioxidant activity of fractions of water, ethyl acetate, and n-hexane were 9.42±1.16%, 21.35±1.86%, and 67.40±0.82% mg GA equivalent/g fraction and 29.46 μg/ml±0.99%, 7.08 μg/ml±0.39%, and 10.90 μg/ml±1.05%, respectively.Conclusion: Fraction of ethyl acetate exhibited the highest antioxidant activity and TPC. This study showed that there were significant correlations between TPC and antioxidant activity at significance level p<0.05.

scholarly journals Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract of Justicia procumbans leaf

2021 ◽  
Vol 7 (1) ◽  
pp. 41-45
Author(s):  
MY Agrawal ◽  
◽  
YP Agrawal ◽  
SK Arora ◽  
P Lahange ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Assay Method ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Hydroalcoholic Extract

Objective: To perform Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract Justicia procumbans leaf. Methods: Proximate analysis in terms of ash value, extractive value was performed as per the standard method. Presence of secondary metabolites in the extract of Justicia procumbans was performed through various chemical tests. Folin-Ciocalteu assay method was used to determine total phenolic content and the in-vitro antioxidant activity was investigated in a dose-dependent manner with the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide free radical scavenging method. Results: Present studies revealed that the hydro-alcoholic extract of leaf contains secondary metabolites such as alkaloids, steroids, flavonoids, carbohydrates, proteins, and tannins. Total phenolic content was found to be 79.32±0.02 mg/gm by using the Linear Equation. IC50 value by DPPH and H2O2 methods was found to be 68.83µg/ml and 56.02 µg/ml respectively. Conclusion: It has been observed that the plant has high phenolic compounds and antioxidant activity. Therefore his plant can be the potent source of natural antioxidants as compared to synthetic compounds.

Sign in / Sign up

Export Citation Format

Share Document