scholarly journals Viability of seedling image analysis (Vigor-S) to determine the physiological potential of melon seeds

2020 ◽  
Vol 42 ◽  
Author(s):  
Moadir de Sousa Leite ◽  
Salvador Barros Torres ◽  
Francisco Guilhien Gomes Junior ◽  
Carlos Henrique Queiroz Rego ◽  
Emanoela Pereira de Paiva ◽  
...  
Keyword(s):  
Image Analysis ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Nacl Solution ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Analysis System ◽  
Melon Seeds

Abstract: Melon is a vegetable crop of food and nutritional importance whose cultivation is growing worldwide. In this sense, investments in technologies are necessary, especially regarding the quality of seeds used in the establishment of new areas. This study aimed to verify the effectiveness of the Automated Seed Vigor Analysis System (Vigor-S) in the evaluation of the physiological potential of melon seeds. Eight seed lots (four lots of the hybrid Supreme and four of the hybrid Premier) were used. The physiological potential of the seeds was evaluated through tests indicated for analyzing the quality of melon seeds associated with the image analysis by using the software Vigor-S, which included the analysis of seedlings from the germination test and accelerated aging in saturated NaCl solution. The experiment was conducted in a completely randomized design, with five replications. The results indicated the effectiveness of Vigor-S, especially when it was used associated with the accelerated aging test in saturated NaCl solution, with results similar to those obtained in traditional vigor tests. Thus, the Automated Seed Vigor Analysis System (Vigor-S) was effective in evaluating the physiological potential of melon seeds.

scholarly journals Accelerated aging of melon seeds

2003 ◽  
Vol 60 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Salvador Barros Torres ◽  
Julio Marcos Filho
Keyword(s):  
Salt Solution ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Traditional Procedure ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Melon Seeds

Accelerated aging is one of the most useful tests used for the evaluation of seed vigor but it is seldomly used to test melon (Cucumis melo L.) seeds. The objective this research was to compare different procedures of the accelerated aging test to evaluate the physiological quality of melon seeds and the efficiency of using saturated salt solution for the control of water uptake by seeds. Five seed lots each of the hybrids AF-646 and AF-682 were tested for germination, seedling emergence, traditional accelerated aging (periods 0f 48, 72 and 96 hours, at 38 or 41°C) and salt saturated accelerated aging. The accelerated aging test (traditional procedure and with salt solution) for 72h and 96h, at 38 or 41°C was sensitive to detect differences in the physiological quality of the seeds. It was also observed that the seed water content after salt saturated accelerated aging was lower and more uniform, thus presenting advantagens in relation to the traditional procedure.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Physiological quality of popcorn seeds assessed by the accelerated aging test

2018 ◽  
Vol 40 (4) ◽  
pp. 428-434 ◽  
Author(s):  
Cristiane da Silva Rocha ◽  
Hugo César Rodrigues Moreira Catão ◽  
Franciele Caixeta ◽  
Heloisa Karoline Kavan ◽  
Talis Melo Claudino ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Moisture Content ◽  
Accelerated Aging ◽  
Seed Moisture Content ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Consistent Information

Abstract: Despite the insufficient internal supply of popcorn kernel cultivars, the Brazilian production is continuously increasing. Therefore, searching for tests that offer fast results and reliable information on the physiological potential of the seeds is very important. The objective of this study was to assess the efficiency of the accelerated aging test, by using different times and exposure temperatures, for the evaluation of the physiological quality of popcorn seed lots. Popcorn seeds from three lots of the hybrid AP 8203 were used. For the initial characterization of the lots, the following tests were conducted: seed moisture content, first and final germination count, and field emergence. The accelerated aging test was performed in a 3x4x2 factorial scheme. After aging, the seeds were tested for moisture content, germination, and electrical conductivity. The experiment was carried out in a completely randomized design. The characteristic vigor was affected during aging, as evidenced by the increase in the amount of leachate detected by the electrical conductivity test. Seed lot 3 was most vigorous in the aging test conducted for 48 h at 45 °C. The accelerated aging test at 42 ºC for 48 h provides consistent information to differentiate seed lots of popcorn.

scholarly journals Vigor testings for evaluating the physiological quality of chia seeds

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2891
Author(s):  
Fernanda Brito Cardoso ◽  
Carlos Henrique Queiroz Rego ◽  
Izabela Cristina de Oliveira ◽  
Alan Mario Zuffo ◽  
Ana Carina da Silva Cândido ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Traditional Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Distilled Water ◽  
Nacl Solution ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>

scholarly journals Piptadenia moniliformis Benth. seeds subjected to accelerated aging

2021 ◽  
Vol 6 (1) ◽  
pp. 100-107
Author(s):  
Gilvaneide Alves de Azerêdo ◽  
Rinaldo Cesar de Paula
Keyword(s):  
Sulfuric Acid ◽  
Tree Species ◽  
Accelerated Aging ◽  
Forest Tree ◽  
Seed Vigor ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Concentrated Sulfuric Acid ◽  
Round Wire

The accelerated aging test is efficient for evaluating the seed vigor of many  species, but there are few studies on forest tree species. The aim of this study was to determine the adequate exposure time of Piptadenia moniliformis Benth. seeds to the accelerated aging test in order to evaluate the vigor of seeds classified by color and size. Ten sub-lots were obtained by separating the seeds into two tegument colors (light and dark) and five size classes (11, 12, 13, 14, and 15), using round wire sieves. Before the  accelerated aging (AA) test, the seeds were treated with concentrated sulfuric acid for 20 minutes to overcome dormancy. Next, the sub-lot seeds underwent different aging times (0, 24, 48, 72, 96 and 120 hours) at 45 ºC. The  AA test, conducted at 45 ºC for 72 hours, is adequate to evaluate the quality of P. moniliformis seeds, in terms of color and size.

scholarly journals Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor)

2017 ◽  
Vol 1 (1) ◽  
pp. 6-12 ◽  
Author(s):  
KHODRATIEN FATONAH ◽  
IRFAN SULIANSYAH ◽  
NALWIDA ROZEN
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Test Method ◽  
Water Volume ◽  
Conductivity Method ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Potassium Leakage

Fatonah K, Suliansyah I, Rozen N. 2017. Electrical conductivity for seed vigor test in sorghum (Sorghum bicolor). Cell Biol Dev 1: 6-12. The objectives of this study were to obtain electrical conductivity test method for seed vigor test in sorghum, to recognize relationship between electrical conductivity test and potassium leakage, and to recognize relationship between electrical conductivity test and other variable on seed vigor. This study have two step experiments. The objective of Experiment I was to determine accurate combinations of water volume and amount seed of the electrical conductivity test for seed vigor test in sorghum. Completely Randomized Design of 15 seed lots with 3 replications were used to determine electrical conductivity method with different vigor. Experiment II were to test electrical conductivity method of 21 seed lots of sorghum without accelerated aging test and 21 seed lots of sorghum were given accelerated aging test. Completely Randomized Design of 21 seed lots with 3 replications were used in Experiment II. The variable were observed : standard germination, field emergence, speed of germination, first count, conductivity, potassium leakage and eight combination conductivity method of water volume (50, 100, 150 and 200 ml) and amount of seed sorghum (50 and 75 seed count). The result of this experiments showed that electrical conductivity test method with 150 ml water volume and 75 seed count was accurate and suitable for sorghum seed vigor test; electrical conductivity test showed positive correlation with potassium leakage;and electrical conductivity test can be used for seed vigor test in sorghum and provided the potential of physiological seed were shown through : standard germination test, field emergence test, first count test and speed of germination with negative correlation.

scholarly journals Computerized image analysis of seedlings to evaluate broccoli seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Haynna Fernandes Abud ◽  
Silvio Moure Cicero ◽  
Francisco Guilhien Gomes Junior
Keyword(s):  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Computer Image Analysis ◽  
Computerized Image Analysis ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract: The computerized image analysis of seedlings constitutes an efficient technique to evaluate the quality of seed lots, since it is quick in responding, has a simple execution and reproduction. The goal of this research was to verify the efficiency of the computer image analysis of seedlings to detect vigor differences among broccoli seed lots and compare them with other traditionally used vigor tests. Computerized image analysis were performed by the Seed Vigor Imaging System (SVIS®), using 3-day-old seedlings and the results were compared to germination at first count, seedling emergence, accelerated aging and electrical conductivity tests. Evaluations were performed in three experimental periods (before storage, 6 and 12 months of storage at 20 °C and 45-50% RH). A completely randomized design with eight replications of 25 seeds was used. Data were submitted to analysis of variance and means were compared by Scott-Knott test at 5% error probability. It is concluded that it is possible to detect vigor differences among broccoli seed lots by the computerized image analysis of seedlings with SVIS®, similarly to traditional vigor tests.

Accelerated aging test and antioxidant enzyme activity to assess chickpea seed vigor

2021 ◽  
Vol 43 ◽  
Author(s):  
Joyce de Oliveira Araújo ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Warley Marcos Nascimento ◽  
Auxiliadora Oliveira Martins ◽  
Marcelo Augusto Rocha Limão
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Traditional Method ◽  
Accelerated Aging ◽  
Antioxidant Enzyme Activity ◽  
Seed Vigor ◽  
Nacl Solution ◽  
High Quality ◽  
Accelerated Aging Test ◽  
Aging Test

Abstract: The chickpea crop has been expanding in Brazil and, thus, the demand for high quality seeds along with it. The aim of this study was to adapt the methodology for evaluation of chickpea seed vigor by the accelerated aging test and to analyze whether antioxidant enzyme activity is related to the physiological potential of these seeds. Seeds from five seed lots of the cv. BRS Aleppo and from four seed lots of the cv. Cícero were initially tested to characterize their physiological potential. The accelerated aging test was conducted by the traditional method (100% RH) and with a saturated NaCl solution (76% RH), both at 41 °C, for 24, 36, 48, and 72 h. The activity of the SOD, CAT, and APX enzymes were also determined. The accelerated aging test using 41 °C/48 h with a saturated NaCl solution is recommended for assessment of chickpea seed vigor. The activity of CAT and APX enzymes may be related to the physiological potential of these seeds, but this is not the case with SOD.

scholarly journals Genetic diversity for physiological quality of seeds from corn (Zea mays L.) intervarietal crossbreeds

2013 ◽  
Vol 35 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Rubens Onofre Nodari
Keyword(s):  
Genetic Diversity ◽  
Zea Mays L ◽  
Accelerated Aging ◽  
Genetic Contribution ◽  
Percentage Germination ◽  
Canonical Variables ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

The physiological quality is based on the genotype and may be accompanied since the first stages of selection through the study of genetic diversity. The purpose of this study was to evaluate the parental genetic contribution in the seed physiological quality in corn intervarietal crossbreeds. The intervarietal crossbreeds were performed using landraces genotypes, open pollinated varieties and commercial hybrids. The seeds obtained from the crossbreed, their equivalents and parentals were evaluated regarding their water percentage, germination, germination speed rate, accelerated aging, field emergence and electrical conductivity. The seeds obtained in the crossbreed are viable and vivid with heterosis when compared with the parentals. The accelerated aging test was more effective for selecting the strongest genotypes. The canonical variables were useful to group the genotypes that presented similar physiological characteristics. The genotypes MPA 01 x Pixurum 05, MPA 01 x AS1565, MPA 01 x SJC5886, SJC5886 x Pixurum 05 and Fundacep 35 x SCS 154 Fortuna were the best crossbreeds regarding the production of better physiological quality seeds.

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