scholarly journals Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development

Sociobiology ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 5935
Author(s):  
Xu Jiang He ◽  
Hao Wei ◽  
Wu Jun Jiang ◽  
Yi Bo Liu ◽  
Xiao Bo Wu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Apis Mellifera ◽  
Signaling Pathways ◽  
Maternal Effect ◽  
Caste Differentiation ◽  
Hippo Signaling ◽  
Thorax Length ◽  
Queen Cell ◽  
Epigenetic Analysis ◽  
Worker Caste

Queen-worker caste dimorphism is a typical trait for honeybees (Apis mellifera). We previously showed a maternal effect on caste differentiation and queen development, where queens emerged from queen-cell eggs (QE) had higher quality than queens developed from worker cell eggs (WE). In this study, newly-emerged queens were reared from QE, WE, and 2-day worker larvae (2L). The thorax size and DNA methylation levels of queens were measured. We found that queens emerging from QE had significantly larger thorax length and width than WE and 2L. Epigenetic analysis showed that QE/2L comparison had the most different methylated genes (DMGs, 612) followed by WE/2L (473), and QE/WE (371). Interestingly, a great number of DMGs (42) were in genes belonging to mTOR, MAPK, Wnt, Notch, Hedgehog, FoxO, and Hippo signaling pathways that are involved in regulating caste differentiation, reproduction and longevity. This study proved that honeybee maternal effect causes epigenetic alteration regulating caste differentiation and queen development.

scholarly journals Effects of Queen Cell Size and Caging Days of Mother Queen on Rearing Young Honey Bee Queens Apis mellifera L.

2018 ◽  
Vol 62 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Xiaobo Wu ◽  
Linbin Zhou ◽  
Chuibin Zou ◽  
Zhijiang Zeng
Keyword(s):  
Birth Weight ◽  
Apis Mellifera ◽  
Cell Size ◽  
Egg Size ◽  
Expression Level ◽  
Thorax Length ◽  
Reproductive Ability ◽  
Queen Cell ◽  
Young Queen ◽  
Honey Bee Queens

Abstract This study aims to investigate the effect of queen cell size (9.4 mm, 9.6 mm, 9.8 mm and 10.0 mm) and mother queen caged time (0 day, 2 days and 4 days) on rearing young queens without grafting larvae. The birth weight, ovarian tubes, thorax length and width were significantly increased with the increasing diameter of queen cell size. The expression level of Vitellogenin (Vg) in young queen ovaries was also up-regulated with the increased queen cell size diameter. These results indicate that the queen cell size can strongly affect the rearing queen quality and reproductive ability. Moreover, the weight, length and width of laying eggs rose with the mother queen caging time, and young queens reared with the hatched larvae from these eggs were also increased in terms of birth weight, ovarian tubes, thorax length and width. Furthermore, the expression level of Vg in reared queen ovaries was also up-regulated with the caged time. These results reveal that the caged time of queens could significantly influence egg size and their relative queen quality.

Genome-scale sequence data processing and epigenetic analysis of DNA methylation

2013 ◽  
Vol 35 (6) ◽  
pp. 685-694
Author(s):  
Ting-Zhang WANG ◽  
Gao SHAN ◽  
Jian-Hong XU ◽  
Qing-Zhong XUE
Keyword(s):  
Dna Methylation ◽  
Data Processing ◽  
Sequence Data ◽  
Epigenetic Analysis ◽  
Genome Scale ◽  
Scale Sequence

scholarly journals Presence and Prevalence of Viruses in Local and Migratory Honeybees (Apis mellifera) in Massachusetts

2009 ◽  
Vol 75 (24) ◽  
pp. 7862-7865 ◽  
Author(s):  
Anna Welch ◽  
Francis Drummond ◽  
Sunil Tewari ◽  
Anne Averill ◽  
John P. Burand
Keyword(s):  
Apis Mellifera ◽  
Black Queen Cell Virus ◽  
Deformed Wing Virus ◽  
Sacbrood Virus ◽  
Queen Cell ◽  
The Impact

ABSTRACT Migratory and local bees in Massachusetts were analyzed for seven viruses. Three were detected: black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). DWV was most common, followed closely by BQCV and then by SBV. BQCV and SBV were present at significantly higher rates in the migratory bees assayed, bringing into question the impact that these bees have on the health of local bee populations.

ADAMTS9-AS2: A functional long non-coding RNA in tumorigenesis

2021 ◽  
Vol 27 ◽  
Author(s):  
Wen Xu ◽  
Bei Wang ◽  
Yuxuan Cai ◽  
Jinlan Chen ◽  
Xing Lv ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Signaling Pathways ◽  
Therapeutic Target ◽  
Molecular Mechanisms ◽  
Human Cancer ◽  
Migration Inhibition ◽  
Cancer Proliferation ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Background: Long non-coding RNAs (lncRNA) have been identified as novel molecular regulators in cancers. LncRNA ADAMTS9-AS2 can mediate the occurrence and development of cancer through various ways such as regulating miRNAs, activating the classical signaling pathways in cancer, and so on, which have been studied by many scholars. In this review, we summarize the molecular mechanisms of ADAMTS9-AS2 in different human cancers. Methods: Through a systematic search of PubMed, lncRNA ADAMTS9-AS2 mediated molecular mechanisms in cancer are summarized inductively. Results: ADAMTS9-AS2 aberrantly expression in different cancers is closely related to cancer proliferation, invasion, migration, inhibition of apoptosis. The involvement of ADAMTS9-AS2 in DNA methylation, mediating PI3K / Akt / mTOR signaling pathways, regulating miRNAs and proteins, and such shows its significant potential as a therapeutic cancer target. Conclusion: LncRNA ADAMTS9-AS2 can become a promising biomolecular marker and a therapeutic target for human cancer.

scholarly journals TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in zebrafish

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Jonathan Fillatre ◽  
Jean-Daniel Fauny ◽  
Jasmine Alexandra Fels ◽  
Cheng Li ◽  
Mary Goll ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Signaling Pathways ◽  
Transcriptional Control ◽  
Hippo Pathway ◽  
Hippo Signaling ◽  
Zebrafish Model ◽  
Transcription Cofactor ◽  
Expression Of Genes ◽  
Epigenetic Programming ◽  
Left Right Asymmetry

In many vertebrates, establishment of Left-Right (LR) asymmetry results from the activity of a ciliated organ functioning as the LR Organizer (LRO). While regulation of the formation of this structure by major signaling pathways has been described, the transcriptional control of LRO formation is poorly understood. Using the zebrafish model, we show that the transcription factors and cofactors mediating or regulating the transcriptional outcome of the Hippo signaling pathway play a pivotal role in controlling the expression of genes essential to the formation of the LRO including ligands and receptors of signaling pathways involved in this process and most genes required for motile ciliogenesis. Moreover, the transcription cofactor, Vgll4l regulates epigenetic programming in LRO progenitors by controlling the expression of writers and readers of DNA methylation marks. Altogether, our study uncovers a novel and essential role for the transcriptional effectors and regulators of the Hippo pathway in establishing LR asymmetry.

scholarly journals Reliable tumor detection by whole-genome methylation sequencing of cell-free DNA in cerebrospinal fluid of pediatric medulloblastoma

2020 ◽  
Vol 6 (42) ◽  
pp. eabb5427
Author(s):  
Jia Li ◽  
Sibo Zhao ◽  
Minjung Lee ◽  
Yue Yin ◽  
Jin Li ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cerebrospinal Fluid ◽  
Low Frequency ◽  
Pediatric Medulloblastoma ◽  
Oncogenic Mutations ◽  
Genome Methylation ◽  
Epigenetic Analysis ◽  
Public Datasets ◽  
Pediatric Brain ◽  
Normal Cerebellum

Medulloblastoma (MB), the most common form of pediatric brain malignancy, has a low frequency of oncogenic mutations but pronouncedly abnormal DNA methylation changes. Epigenetic analysis of circulating cell-free tumor DNA (ctDNA) by liquid biopsy offers an approach for real-time monitoring of tumor status without tumor dissection. In this study, we identified 6598 differentially methylated CpGs in both MB tumors and the ctDNA isolated from matched cerebrospinal fluid (CSF) compared with normal cerebellum, which could be used to detect MB tumor occurrence and determine its subtype. Furthermore, DNA methylation changes in serial CSF samples could be used to monitor the treatment response and tumor recurrence. Integrating our data with large public datasets, we identified reliable MB DNA methylation signatures in ctDNA that have potential diagnostic and prognostic values. Our study sets the stage for exploiting epigenetic markers in CSF to improve the clinical management of patients with MB.

scholarly journals Diversity and Global Distribution of Viruses of the Western Honey Bee, Apis mellifera

Insects ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 239 ◽  
Author(s):  
Alexis Beaurepaire ◽  
Niels Piot ◽  
Vincent Doublet ◽  
Karina Antunez ◽  
Ewan Campbell ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
High Throughput Sequencing ◽  
Temporal Dynamics ◽  
Pollination Services ◽  
Colony Losses ◽  
New Host ◽  
Deformed Wing Virus ◽  
Bee Colony ◽  
Queen Cell

In the past centuries, viruses have benefited from globalization to spread across the globe, infecting new host species and populations. A growing number of viruses have been documented in the western honey bee, Apis mellifera. Several of these contribute significantly to honey bee colony losses. This review synthetizes the knowledge of the diversity and distribution of honey-bee-infecting viruses, including recent data from high-throughput sequencing (HTS). After presenting the diversity of viruses and their corresponding symptoms, we surveyed the scientific literature for the prevalence of these pathogens across the globe. The geographical distribution shows that the most prevalent viruses (deformed wing virus, sacbrood virus, black queen cell virus and acute paralysis complex) are also the most widely distributed. We discuss the ecological drivers that influence the distribution of these pathogens in worldwide honey bee populations. Besides the natural transmission routes and the resulting temporal dynamics, global trade contributes to their dissemination. As recent evidence shows that these viruses are often multihost pathogens, their spread is a risk for both the beekeeping industry and the pollination services provided by managed and wild pollinators.

Sign in / Sign up

Export Citation Format

Share Document