Uterine epithelial cell release of MIP3, TNF and TGF in response to Escherichia coli, Lactobicillus rhamnosus and pathogen associated molecular patterns (PAMP) and the influence of estradiol on cytokine release.

2004 ◽  
Author(s):  
Margaret A. Crane-Godreau
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Cytokine Release ◽  
Uterine Epithelial Cell ◽  
Cell Release ◽  
Molecular Patterns

scholarly journals Solubility assessment of single-chain antibody fragment against epithelial cell adhesion molecule extracellular domain in four Escherichia coli strains

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Fatemeh Sadat Javadian ◽  
Majid Basafa ◽  
Aidin Behravan ◽  
Atieh Hashemi
Keyword(s):  
Escherichia Coli ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Extracellular Domain ◽  
Epithelial Cell Adhesion Molecule ◽  
Single Chain ◽  
E Coli ◽  
The Impact

Abstract Background Overexpression of the EpCAM (epithelial cell adhesion molecule) in malignancies makes it an attractive target for passive immunotherapy in a wide range of carcinomas. In comparison with full-length antibodies, due to the small size, the scFvs (single-chain variable fragments) are more suitable for recombinant expression in E. coli (Escherichia coli). However, the proteins expressed in large amounts in E. coli tend to form inclusion bodies that need to be refolded which may result in poor recovery of bioactive proteins. Various engineered strains were shown to be able to alleviate the insolubility problem. Here, we studied the impact of four E. coli strains on the soluble level of anti-EpEX-scFv (anti-EpCAM extracellular domain-scFv) protein. Results Although results showed that the amount of soluble anti-EpEX-scFv obtained in BL21TM (DE3) (114.22 ± 3.47 mg/L) was significantly higher to those produced in the same condition in E. coli RosettaTM (DE3) (71.39 ± 0.31 mg/L), and OrigamiTM T7 (58.99 ± 0.44 mg/L) strains, it was not significantly different from that produced by E. coli SHuffleTM T7 (108.87 ± 2.71 mg/L). Furthermore, the highest volumetric productivity of protein reached 318.29 ± 26.38 mg/L in BL21TM (DE3). Conclusions Although BL21TM (DE3) can be a suitable strain for high-level production of anti-EpEX-scFv protein, due to higher solubility yield (about 55%), E. coli SHuffleTM T7 seems to be better candidate for soluble production of scfv compared to BL21TM (DE3) (solubility yield of about 30%).

scholarly journals Sex hormone modulation of human uterine epithelial cell immune responses

2006 ◽  
Vol 46 (6) ◽  
pp. 1082-1087 ◽  
Author(s):  
J. V. Fahey ◽  
T. M. Schaefer ◽  
C. R. Wira
Keyword(s):  
Epithelial Cell ◽  
Immune Responses ◽  
Sex Hormone ◽  
Uterine Epithelial Cell ◽  
Hormone Modulation

scholarly journals Membrane Vesicles Released by a hypervesiculating Escherichia coli Nissle 1917 tolR Mutant Are Highly Heterogeneous and Show Reduced Capacity for Epithelial Cell Interaction and Entry

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0169186 ◽  
Author(s):  
Carla Pérez-Cruz ◽  
María-Alexandra Cañas ◽  
Rosa Giménez ◽  
Josefa Badia ◽  
Elena Mercade ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Membrane Vesicles ◽  
Cell Interaction ◽  
Escherichia Coli Nissle 1917 ◽  
Escherichia Coli Nissle

M1810 Interferon Gamma-Induced STAT1 Phosphorylation is Blocked by Adherent-Invasive Escherichia coli in Human Intestine Epithelial Cell Lines

2010 ◽  
Vol 138 (5) ◽  
pp. S-424
Author(s):  
Juan C. Ossa ◽  
Nathan Ho ◽  
Eytan Wine ◽  
Nelly Leung ◽  
Scott D. Gray-Owen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cell ◽  
Cell Lines ◽  
Interferon Gamma ◽  
Human Intestine ◽  
Epithelial Cell Lines

Mucins and Proteoglycans as Modulators of Embryo-Uterine Epithelial Cell Attachment

1995 ◽  
pp. 103-112 ◽  
Author(s):  
Daniel D. Carson ◽  
JoAnne Julian ◽  
Shouchun Liu ◽  
Larry Rohde ◽  
Gulnar Surveyor ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Attachment ◽  
Uterine Epithelial Cell
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