scholarly journals Ex vivo hypercellular parathyroid gland differentiation using dynamic optical contrast imaging (DOCI)

2021 ◽  
Author(s):  
Shan Huang ◽  
Yazeed Alhiyari ◽  
Yong Hu ◽  
Kenric Tam ◽  
Albert Han ◽  
...  
Keyword(s):  
Parathyroid Gland ◽  
Ex Vivo ◽  
Contrast Imaging ◽  
Optical Contrast

Dynamic Optical Contrast Imaging: A Technique to Differentiate Parathyroid Tissue from Surrounding Tissues

2017 ◽  
Vol 156 (3) ◽  
pp. 480-483 ◽  
Author(s):  
Irene A. Kim ◽  
Zachary D. Taylor ◽  
Harrison Cheng ◽  
Christine Sebastian ◽  
Ashkan Maccabi ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Parathyroid Tissue ◽  
Fluorescence Decay ◽  
Tissue Type ◽  
Acquisition Time ◽  
Contrast Imaging ◽  
Optical Contrast ◽  
Tissue Properties ◽  
Parathyroid Lesions ◽  
Specific Tissue

The variable location and indistinct features of parathyroid glands can make their intraoperative identification challenging. Currently, there exists no routine use of localization methods during surgery. Dynamic optical contrast imaging (DOCI) leverages a novel realization of temporally dependent measurements of tissue autofluorescence that allows the acquisition of specific tissue properties. A prospective series of patients with primary hyperparathyroidism was examined. Parathyroid lesions and surrounding tissues were collected; fluorescence decay images were acquired via DOCI. Ex vivo samples (81 patients) were processed for histologic assessment. DOCI extracts relative fluorescence decay information in a surgically relevant field of view with a clinically accessible acquisition time <2 minutes. Analysis of DOCI revealed microscopic characterization sufficient for tissue type identification consistent with histology ( P < .05). DOCI is capable of efficiently distinguishing parathyroid tissue from adjacent tissues. Such an intraoperative tool would be transformative, helping surgeons to identify lesions, preserve healthy tissue, and improve patient outcomes.

scholarly journals Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study

2020 ◽  
Vol 10 ◽  
Author(s):  
Wei Huang ◽  
Jian Lu ◽  
Rongbiao Tang ◽  
Zhiyuan Wu ◽  
Qingbing Wang ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
Ex Vivo Study

scholarly journals Evaluation to Differentiate between Tumor Lesions and the Parenchyma in Partial Nephrectomies for Renal Tumors Based on Quantitative Fluorescence Imaging Using Indocyanine Green Dye

2019 ◽  
Vol 13 (2) ◽  
pp. 74-81
Author(s):  
Norihito Soga ◽  
Akihito Inoko ◽  
Jun Furusawa ◽  
Yuji Ogura
Keyword(s):  
Indocyanine Green ◽  
Success Rate ◽  
Fluorescence Imaging ◽  
Partial Nephrectomy ◽  
Near Infrared ◽  
Clear Cell ◽  
Ex Vivo ◽  
Renal Tumors ◽  
Contrast Imaging ◽  
Malignant Lesions

Introduction: Near-infrared fluorescence imaging with indocyanine green is a useful tool during partial nephrectomy. Because an accurate method for judging hasn't been established yet, the success rate may be slightly different and inconsistent. Materials and Methods: A total of 21 cases with suspected renal cancers who had undergone a partial nephrectomy were enrolled. We examined differences in the success rate between malignant lesions and the parenchyma by quantifying fluorescence in the pre-resection and ex vivo phases. Results: Pre-resection imaging showed a significant degradation of fluorescence in the focused lesion in 76.2% (16/21) of cases. A significant degradation was observed in 73.7% (14/19) of the total malignant lesions, 70.5% (12/17) of cases with a clear cell lesion, 100% (2/2) of cases with non-clear cell lesions, and 100% (2/2) of benign angiomyolipomas. In contrast, imaging of the ex vivo resected specimens showed a significant degradation in fluorescence of the focused lesions in 85.7% (18/21) of cases. A significantly degradation was observed in 84.2% (16/19) of the total malignant lesions, 82.3% (14/17) of cases with a clear cell lesion, 100% (2/2) of cases with non-clear cell lesions, and 100% (2/2) of benign angiomyolipomas. Conclusion: We firstly evaluated the efficacy of quantitative indocyanine green-based fluorescence as an objective method.

Abstract C83: Dynamic Optical Contrast Imaging (DOCI): Oral screening in the underserved

2018 ◽  
Author(s):  
Peter A. Pellionisz ◽  
Cheng Harrison ◽  
Zachary D. Taylor ◽  
Warren Grundfest ◽  
Maie A. St. John
Keyword(s):  
Contrast Imaging ◽  
Optical Contrast ◽  
Oral Screening

Method for motion artifact compensation in dynamic optical contrast imaging

2020 ◽  
Vol 106 (5) ◽  
pp. 1171-1172
Author(s):  
Y. Hu ◽  
M. St John ◽  
P. Pellionisz ◽  
S. Moon ◽  
Y.M. Alhiyari
Keyword(s):  
Motion Artifact ◽  
Contrast Imaging ◽  
Optical Contrast

Minimally Invasive Parathyroid Surgery, The Norman 20% Rule: Is It Valid?

2011 ◽  
Vol 77 (4) ◽  
pp. 484-487 ◽  
Author(s):  
Amy R. Quillo ◽  
Jeffery M. Bumpous ◽  
Richard E. Goldstein ◽  
Muffin M. Fleming ◽  
Ccrp ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Primary Hyperparathyroidism ◽  
Minimally Invasive ◽  
Parathyroid Gland ◽  
Ex Vivo ◽  
Frozen Section ◽  
Postoperative Management ◽  
Average Percentage ◽  
Parathyroid Hormone Monitoring ◽  
Intraoperative Parathyroid Hormone

The 20 per cent rule proposed by Norman established a guideline using radioactivity in the minimally invasive radioguided parathyroidectomy (MIRP) technique to localize and confirm removal of an abnormal parathyroid gland in patients with primary hyperparathyroidism. If radioactivity in the resected gland was at least 20 per cent of excision site/background radioactivity, the 20 per cent rule was satisfied. Patients meeting these criteria underwent unilateral MIRP without intraoperative parathyroid hormone assay or intraoperative frozen section. The study aim was to independently evaluate the 20 per cent rule in MIRP patients with primary hyperparathyroidism. Using the University of Louisville Parathyroid Database from January 1, 1999 to December 31, 2007, 216 MIRP patients with complete radioguided and postoperative management data were identified. The average percentage of ex vivo parathyroid gland radioactivity compared with excision site/background radioactivity was 107 per cent with a range from 14 to 388 per cent. For 99 per cent (196/198) radioactivity recorded from the excised gland was at least 20 per cent of radioactivity recorded from the excision site. Normocalcemia was documented in 98.5 per cent (195/198) at 12 month follow-up. Our data supports the 20 per cent rule in that in 99 per cent of MIRP patients the resected gland radioactivity was at least 20 per cent of excision site radioactivity allowing localization and confirmation of an overactive gland without intraoperative parathyroid hormone monitoring or tissue analysis.

A Tool to Locate Parathyroid Glands Using Dynamic Optical Contrast Imaging

2021 ◽  
Author(s):  
Yong Hu ◽  
Albert Y. Han ◽  
Shan Huang ◽  
Peter Pellionisz ◽  
Yazeed Alhiyari ◽  
...  
Keyword(s):  
Parathyroid Glands ◽  
Contrast Imaging ◽  
Optical Contrast

Synchrotron inline phase contrast µCT enables detailed virtual histology of embedded soft-tissue samples with and without staining

2018 ◽  
Vol 25 (4) ◽  
pp. 1153-1161 ◽  
Author(s):  
Mara Saccomano ◽  
Jonas Albers ◽  
Giuliana Tromba ◽  
Marina Dobrivojević Radmilović ◽  
Srećko Gajović ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Soft Tissue ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Strong Increase ◽  
Micro Computed Tomography ◽  
Contrast Imaging ◽  
Specific Staining ◽  
Tissue Samples ◽  
Virtual Histology

Synchrotron radiation micro-computed tomography (SRµCT) based virtual histology, in combination with dedicated ex vivo staining protocols and/or phase contrast, is an emerging technology that makes use of three-dimensional images to provide novel insights into the structure of tissue samples at microscopic resolution with short acquisition times of the order of minutes or seconds. However, the high radiation dose creates special demands on sample preparation and staining. As a result of the lack of specific staining in virtual histology, it can supplement but not replace classical histology. Therefore, the aim of this study was to establish and compare optimized ex vivo staining and acquisition protocols for SRµCT-based virtual histology of soft-tissue samples, which could be integrated into the standard workflow of classical histology. The high grade of coherence of synchrotron radiation allows the application of propagation-based phase contrast imaging (PBI). In this study, PBI yielded a strong increase in image quality even at lower radiation doses and consequently prevented any damage to the tissue samples or the embedding material. This work has demonstrated that the improvement in contrast-to-noise ratio by PBI enabled label-free virtual histology of soft-tissue specimens embedded in paraffin to a level of detail that exceeds that achieved with staining protocols.

scholarly journals Feasibility of an Inversion Recovery-Prepared Fat-Saturated Zero Echo Time Sequence for High Contrast Imaging of the Osteochondral Junction

2021 ◽  
Vol 12 ◽  
Author(s):  
Hyungseok Jang ◽  
Yajun Ma ◽  
Michael Carl ◽  
Alecio F. Lombardi ◽  
Eric Y. Chang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Ex Vivo ◽  
Inversion Recovery ◽  
High Contrast ◽  
Contrast Imaging ◽  
Tibial Cartilage ◽  
High Contrast Imaging ◽  
Echo Time ◽  
Adiabatic Inversion

PurposeThe osteochondral junction (OCJ) region—commonly defined to include the deep radial uncalcified cartilage, tidemark, calcified cartilage, and subchondral bone plate—functions to absorb mechanical stress and is commonly associated with the pathogenesis of osteoarthritis. However, magnetic resonance imaging of the OCJ region is difficult due to the tissues’ short transverse relaxation times (i.e., short T2 or T2*), which result in little or no signal with conventional MRI. The goal of this study is to develop a 3D adiabatic inversion recovery prepared fat saturated zero echo time (IR-FS-ZTE) sequence for high-contrast imaging of the OCJ.MethodAn IR-FS-ZTE MR sequence was developed to image the OCJ on a clinical 3T MRI scanner. The IR-FS-ZTE sequence employed an adiabatic inversion pulse followed by a fat saturation pulse that suppressed signals from the articular cartilage and fat. At an inversion time (TI) that was matched to the nulling point of the articular cartilage, continuous ZTE imaging was performed with a smoothly rotating readout gradient, which enabled time-efficient encoding of the OCJ region’s short T2 signal with a minimal echo time (TE) of 12 μs. An ex vivo experiment with six cadaveric knee joints, and an in vivo experiment with six healthy volunteers and three patients with OA were performed to evaluate the feasibility of the proposed approach for high contrast imaging of the OCJ. Contrast-to-noise ratios (CNRs) between the OCJ and its neighboring femoral and tibial cartilage were measured.ResultsIn the ex vivo experiment, IR-FS-ZTE produced improved imaging of the OCJ region over the clinical sequences, and significantly improved the contrast compared to FS-ZTE without IR preparation (p = 0.0022 for tibial cartilage and p = 0.0019 for femoral cartilage with t-test). We also demonstrated the feasibility of high contrast imaging of the OCJ region in vivo using the proposed IR-FS-ZTE sequence, thereby providing more direct information on lesions in the OCJ. Clinical MRI did not detect signal from OCJ due to the long TE (&gt;20 ms).ConclusionIR-FS-ZTE allows direct imaging of the OCJ region of the human knee and may help in elucidating the role of the OCJ in cartilage degeneration.

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