Optical Sectioning Raman Microscopy

1991 ◽  
Vol 45 (10) ◽  
pp. 1604-1606 ◽  
Author(s):  
Anurag Govil ◽  
David M. Pallister ◽  
Li-Heng Chen ◽  
Michael D. Morris
Keyword(s):  
Benzoic Acid ◽  
Nearest Neighbor ◽  
Raman Microscopy ◽  
Hadamard Transform ◽  
Optical Sectioning ◽  
Focus Information

We describe the use of Hadamard transform Raman microscopy to acquire optically sectioned images of crystals of benzoic acid. Nearest-neighbor deblurring is used to reject out-of-focus information and sharpen the Raman images obtained from the crystal.

Multichannel Hadamard Transform Raman Microscopy

1990 ◽  
Vol 44 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Patrick J. Treado ◽  
Michael D. Morris
Keyword(s):  
Spatial Resolution ◽  
Spectral Resolution ◽  
Spatial Multiplexing ◽  
Raman Microscopy ◽  
Hadamard Transform ◽  
Multichannel Detection ◽  
Spatial Multiplex

Spatial multiplexing is combined with multichannel detection in a Hadamard transform Raman microscope which provides 127 × 128 pixel images with 12 cm−1 spectral resolution. Spatial resolution of 0.6 μm per pixel has been achieved. A spatial multiplex advantage of better that 104 is demonstrated. Instrumental design details and spectroscopic images are presented.

Hadamard Transform Raman Microscopy of Laser-Modified Graphite Electrodes

1990 ◽  
Vol 44 (8) ◽  
pp. 1270-1275 ◽  
Author(s):  
Patrick J. Treado ◽  
Anurag Govil ◽  
Michael D. Morris ◽  
Kent D. Sternitzke ◽  
Richard L. McCreery
Keyword(s):  
Raman Microscopy ◽  
Hadamard Transform ◽  
Graphite Electrodes

Multispectral Hadamard Transform Raman Microscopy

1991 ◽  
Vol 45 (10) ◽  
pp. 1717-1720 ◽  
Author(s):  
Kei-Lee K. Liu ◽  
Li-Heng Chen ◽  
Rong-Sheng Sheng ◽  
Michael D. Morris
Keyword(s):  
Multispectral Imaging ◽  
Raman Microscopy ◽  
Hadamard Transform ◽  
Two Dimensional ◽  
Polymer Laminates ◽  
Video Detector

The Hadamard transform Raman microscope equipped with a two-dimensional video detector is inherently capable of obtaining images at several different Raman frequencies simultaneously. In this communication, we describe the necessary software to exploit this multispectral capability. We apply multispectral imaging to polymer laminates and to removal of artifacts from Raman images.

scholarly journals Computational optical sectioning by phase-space imaging with an incoherent multiscale scattering model

2020 ◽  
Author(s):  
Yi Zhang ◽  
Zhi Lu ◽  
Jiamin Wu ◽  
Xing Lin ◽  
Dong Jiang ◽  
...  
Keyword(s):  
Phase Space ◽  
Fluorescence Imaging ◽  
Superior Performance ◽  
Scattering Medium ◽  
Optical Sectioning ◽  
Entire Volume ◽  
Scattering Model ◽  
Zebrafish Larvae ◽  
Focus Information ◽  
Noisy Background

Optical sectioning is essential for fluorescence imaging in thick tissue to extract in-focus information from noisy background. Traditional methods achieve optical sectioning by rejecting the out-of-focus photons at a cost of photon efficiency, resulting in a tradeoff between sectioning capability and detection parallelization. Here, we show phase-space imaging with an incoherent multiscale scattering model can achieve computational optical sectioning with ~20 dB improvement for signal-to-background ratio in scattering medium, while maximizing the detection parallelization by imaging the entire volume simultaneously. We validated the superior performance by imaging various biological dynamics in Drosophila embryos, zebrafish larvae, and mice.

Observation of Superlattice Dislocations on Cube Planes in Ni3Al

1973 ◽  
Vol 31 ◽  
pp. 174-175 ◽  
Author(s):  
J. M. Oblak ◽  
W. H. Rand
Keyword(s):  
Nearest Neighbor ◽  
Antiphase Boundary ◽  
Nearest Neighbors ◽  
High Energy ◽  
Cross Slip ◽  
Octahedral Plane ◽  
Trapping Mechanism ◽  
Slip Traces

The energy of an a/2 <110> shear antiphase. boundary in the Ll2 expected to be at a minimum on {100} cube planes because here strue ture is there is no violation of nearest-neighbor order. The latter however does involve the disruption of second nearest neighbors. It has been suggested that cross slip of paired a/2 <110> dislocations from octahedral onto cube planes is an important dislocation trapping mechanism in Ni3Al; furthermore, slip traces consistent with cube slip are observed above 920°K.Due to the high energy of the {111} antiphase boundary (> 200 mJ/m2), paired a/2 <110> dislocations are tightly constricted on the octahedral plane and cannot be individually resolved.

Application of Lattice Imaging Techniques to Amorphous Films

1975 ◽  
Vol 33 ◽  
pp. 8-9
Author(s):  
S. R. Herd ◽  
P. Chaudhari
Keyword(s):  
Nearest Neighbor ◽  
Random Network ◽  
Imaging Techniques ◽  
Network Models ◽  
Accurate Method ◽  
Direct Transmission ◽  
Lattice Imaging ◽  
Transmission Electron ◽  
Lattice Planes ◽  
Nearest Neighbor Distances

Electron diffraction and direct transmission have been used extensively to study the local atomic arrangement in amorphous solids and in particular Ge. Nearest neighbor distances had been calculated from E.D. profiles and the results have been interpreted in terms of the microcrystalline or the random network models. Direct transmission electron microscopy appears the most direct and accurate method to resolve this issue since the spacial resolution of the better instruments are of the order of 3Å. In particular the tilted beam interference method is used regularly to show fringes corresponding to 1.5 to 3Å lattice planes in crystals as resolution tests.

TEM characterization of proton-exchanged LiNbO3 optical waveguides

1986 ◽  
Vol 44 ◽  
pp. 480-481
Author(s):  
W. E. Lee
Keyword(s):  
Refractive Index ◽  
Benzoic Acid ◽  
Optical Waveguides ◽  
Total Internal Reflection ◽  
Index Of Refraction ◽  
Optical Measurements ◽  
Lithium Ions ◽  
Wide Channel ◽  
Tem Characterization

An optical waveguide consists of a several-micron wide channel with a slightly different index of refraction than the host substrate; light can be trapped in the channel by total internal reflection.Optical waveguides can be formed from single-crystal LiNbO3 using the proton exhange technique. In this technique, polished specimens are masked with polycrystal1ine chromium in such a way as to leave 3-13 μm wide channels. These are held in benzoic acid at 249°C for 5 minutes allowing protons to exchange for lithium ions within the channels causing an increase in the refractive index of the channel and creating the waveguide. Unfortunately, optical measurements often reveal a loss in waveguiding ability up to several weeks after exchange.

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