Pressure-Tuning FT-IR Study of Human Cervical Tissues

Infrared spectra of the normal connective, the normal epithelial, and the malignant epithelial tissues of cervix from seven patients have been measured as a function of pressure. Extremely high quality spectra of these tissue samples have been obtained. Consequently, structural differences at the molecular level among these three types of cervical tissues have been extracted from their pressure-tuning infrared spectra in the regions of the symmetric and antisymmetric stretching modes of phosphodiester groups, the C-O stretching mode, the CH2 bending mode, and the amide I mode. Significant differences in many features between the infrared spectra of the normal and the malignant cervical tissues and cells suggest that the infrared spectra of exfoliated cells and the biopsy of cervical tissues may be used in rapid evaluation of cervical cancer or in screening of large-volume normal cervical specimens. The infrared spectrum of the normal connective tissue of cervix in the frequency region 950 to 1100 cm−1 is similar to that of the malignant cervical tissue and cells. Therefore, if only this region of the spectrum is examined, the normal connective tissue will be misinterpreted as malignant tissue. However, the normal connective tissue can be differentiated unambiguously from the malignant tissue or the normal epithelial tissue by the infrared spectra in the frequency region 1200 to 1500 cm−1, where several well-defined sharp bands are unique for the normal connective tissue.

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Normal and Malignant Human Colonic Tissues Investigated by Pressure-Tuning FT-IR Spectroscopy

Applied Spectroscopy ◽

10.1366/0003702934065885 ◽

1993 ◽

Vol 47 (11) ◽

pp. 1830-1836 ◽

Cited By ~ 28

Author(s):

Patrick T. T. Wong ◽

Suzanne Lacelle ◽

Hossein M. Yazdi

Keyword(s):

Infrared Spectra ◽

Structural Changes ◽

Human Colon ◽

Rapid Identification ◽

Normal Epithelium ◽

Pressure Tuning ◽

Different Types ◽

Ft Ir ◽

Ft Ir Spectroscopy

Infrared spectra of the epithelial, the connective, the mucosa, and the malignant tissues of the human colon have been measured as a function of pressure. Infrared spectra of collagen proteins have also been measured and compared with the connective tissue. The infrared spectra of different types of colon tissues exhibit significantly different patterns. With these specific infrared patterns, the tissue types of the colon can be differentiated unambiguously. Many structural changes at the molecular level from normal epithelium to malignant tumor have been derived from the spectral features of these two related tissues. These structural changes in carcinogenesis of the colon are comparable with those in other human cancers. The present results suggest that determination of these infrared spectra may be applied to the rapid identification of normal tissue types of the colon and evaluation of colon cancer.

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Alteration of protein expression and spliceosome pathway activity during Barrett’s carcinogenesis

Journal of Gastroenterology ◽

10.1007/s00535-021-01802-2 ◽

2021 ◽

Author(s):

Christoph Stingl ◽

Angela Bureo Gonzalez ◽

Coşkun Güzel ◽

Kai Yi Nadine Phoa ◽

Michail Doukas ◽

...

Keyword(s):

Micro Rna ◽

Differentially Expressed ◽

Epithelial Tissue ◽

Label Free ◽

Tissue Samples ◽

Damage Repair ◽

Inter Observer Variability ◽

Stromal Tissue ◽

The Face ◽

Abundance And Diversity

Abstract Background Barrett’s esophagus (BE) is a known precursor lesion and the strongest risk factor for esophageal adenocarcinoma (EAC), a common and lethal type of cancer. Prediction of risk, the basis for efficient intervention, is commonly solely based on histologic examination. This approach is challenged by problems such as inter-observer variability in the face of the high heterogeneity of dysplastic tissue. Molecular markers might offer an additional way to understand the carcinogenesis and improve the diagnosis—and eventually treatment. In this study, we probed significant proteomic changes during dysplastic progression from BE into EAC. Methods During endoscopic mucosa resection, epithelial and stromal tissue samples were collected by laser capture microdissection from 10 patients with normal BE and 13 patients with high-grade dysplastic/EAC. Samples were analyzed by mass spectrometry-based proteomic analysis. Expressed proteins were determined by label-free quantitation, and gene set enrichment was used to find differentially expressed pathways. The results were validated by immunohistochemistry for two selected key proteins (MSH6 and XPO5). Results Comparing dysplastic/EAC to non-dysplastic BE, we found in equal volumes of epithelial tissue an overall up-regulation in terms of protein abundance and diversity, and determined a set of 226 differentially expressed proteins. Significantly higher expressions of MSH6 and XPO5 were validated orthogonally and confirmed by immunohistochemistry. Conclusions Our results demonstrate that disease-related proteomic alterations can be determined by analyzing minute amounts of cell-type-specific collected tissue. Further analysis indicated that alterations of certain pathways associated with carcinogenesis, such as micro-RNA trafficking, DNA damage repair, and spliceosome activity, exist in dysplastic/EAC.

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Diffuse Reflectance FT-IR Characterization of Active Sites under Reaction Conditions: The Production of Oxygenates in the CO/H2 Reaction

Applied Spectroscopy ◽

10.1366/0003702944027345 ◽

1994 ◽

Vol 48 (10) ◽

pp. 1208-1212 ◽

Cited By ~ 7

Author(s):

J. J. Benítez ◽

I. Carrizosa ◽

J. A. Odriozola

Keyword(s):

Infrared Spectra ◽

Active Sites ◽

Diffuse Reflectance ◽

Reaction Conditions ◽

In Situ Drifts ◽

Ft Ir

The reactivity of a Lu2O3-promoted Rh/Al2O3 catalyst in the CO/H2 reaction is reported. Methane, heavier hydrocarbons, methanol, and ethanol are obtained. In situ DRIFTS has been employed to record the infrared spectra under the actual reaction conditions. The structure of the observed COads DRIFTS bands has been resolved into its components. The production of oxygenates (methanol and ethanol) has been correlated with the results of the deconvolution calculation. Specific sites for the production of methanol and ethanol in the CO/H2 reaction over a Rh,Lu2O3/Al2O3 catalyst are proposed.

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Infrared spectra of aqueous sodium benzoates and salicylates in the carboxyl-stretching region: chelation in aqueous sodium salicylates

Canadian Journal of Chemistry ◽

10.1139/v69-758 ◽

1969 ◽

Vol 47 (24) ◽

pp. 4577-4588 ◽

Cited By ~ 34

Author(s):

G. E. Dunn ◽

R. S. McDonald

Keyword(s):

Salicylic Acid ◽

Carbon Tetrachloride ◽

Infrared Spectra ◽

Aromatic Compounds ◽

Deuterium Oxide ◽

Substituent Effects ◽

Good Evidence ◽

Frequency Region ◽

Aqueous Sodium ◽

Substituent Constants

Infrared spectra in the frequency region 1300–1760 cm−1 are reported for 41 substituted sodium benzoates and 10 substituted sodium salicylates in deuterium oxide solution, and for 9 substituted salicylic acids in chloroform and carbon tetrachloride solutions. Carboxylate stretching frequencies of benzoates and salicylates correlate poorly with substituent constants, but the asymmetric frequencies of benzoates and salicylates correlate well with each other, and the asymmetric frequencies of benzoates correlate well with the asymmetric frequencies of the corresponding nitrobenzenes. It is suggested that, among substituted aromatic compounds, group vibrations which couple with the ring vibrations may correlate well with similar vibrations of other groups, but not with coupled vibrations of different symmetry, uncoupled vibrations, or substituent constants. Chelation in chloroform solutions of salicylic acid dimers can be detected by its influence on substituent effects, but infrared spectra provide no good evidence for chelation in aqueous sodium salicylates.

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An FT-IR Study on Packing Defects in Mixed β-Aggregates of Poly(l-glutamic acid) and Poly(d-glutamic acid): A High-Pressure Rescue from a Kinetic Trap

The Journal of Physical Chemistry B ◽

10.1021/jp2125685 ◽

2012 ◽

Vol 116 (17) ◽

pp. 5172-5178 ◽

Cited By ~ 22

Author(s):

Yudai Yamaoki ◽

Hiroshi Imamura ◽

Aleksandra Fulara ◽

Sławomir Wójcik ◽

Łukasz Bożycki ◽

...

Keyword(s):

High Pressure ◽

Glutamic Acid ◽

Kinetic Trap ◽

Packing Defects ◽

Ft Ir ◽

Ir Study

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AN ENHANCEMENT OF SOLUBILITY OF PIOGLITAZONE HYDROCHLORIDE USING LIQUISOLID TECHNIQUE

INDIAN DRUGS ◽

10.53879/id.50.06.p0036 ◽

2013 ◽

Vol 50 (06) ◽

pp. 36-39

Author(s):

S Deshmane ◽

◽

K Gandhi ◽

S. Nagpure ◽

A. Sawant ◽

...

Keyword(s):

Mathematical Model ◽

Drug Release ◽

Dissolution Rate ◽

In Vivo Study ◽

Dimethyl Acetamide ◽

Volatile Liquid ◽

Ft Ir ◽

Ir Study

The new mathematical model was developed by studying angle of slide using N, N-dimethyl acetamide, non-volatile liquid vehicle and prepared liquisolid tablets, in which the different concentrations of non-volatile liquid adsorbed over carrier and coating material separately. Both DSC and FT-IR study showed better compatibility and stability. The optimized formulation showed higher drug release during in-vitro and in-vivo study against conventional and marketed preparation. The present work concludes that N, N-dimethyl acetamide enhanced the solubility of pioglitazone HCl with higher dissolution rate through liquisolid technique.

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FT-IR study on solvent effects in building blocks of bioactive compounds. V

Journal of Molecular Structure ◽

10.1016/j.molstruc.2005.12.033 ◽

2006 ◽

Vol 790 (1-3) ◽

pp. 89-93

Author(s):

C. Conti ◽

R. Galeazzi ◽

E. Giorgini ◽

G. Tosi

Keyword(s):

Bioactive Compounds ◽

Solvent Effects ◽

Building Blocks ◽

Ft Ir ◽

Ir Study

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Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

Brazilian Dental Journal ◽

10.1590/s0103-64402008000100007 ◽

2008 ◽

Vol 19 (1) ◽

pp. 40-45 ◽

Cited By ~ 4

Author(s):

Ana Teresa Sant'anna ◽

Luis Carlos Spolidório ◽

Lizeti Toledo Oliveira Ramalho

Keyword(s):

Connective Tissue ◽

Chronic Inflammation ◽

Histological Analysis ◽

Subcutaneous Tissue ◽

Experimental Period ◽

Connective Tissues ◽

Plastic Tube ◽

Tissue Samples ◽

Subcutaneous Connective Tissue ◽

Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

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