scholarly journals Fasting Induces the Expression of PGC-1α and ERR Isoforms in the Outer Stripe of the Outer Medulla (OSOM) of the Mouse Kidney

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e26961 ◽  
Author(s):  
Christina T. Teng ◽  
Yin Li ◽  
Pat Stockton ◽  
Julie Foley
Keyword(s):  
Mouse Kidney ◽  
Outer Medulla ◽  
Outer Stripe

scholarly journals Expression of the B splice variant of NBCe1 (SLC4A4) in the mouse kidney

2018 ◽  
Vol 315 (3) ◽  
pp. F417-F428 ◽  
Author(s):  
Lijuan Fang ◽  
Hyun-Wook Lee ◽  
Chao Chen ◽  
Autumn N. Harris ◽  
Michael F. Romero ◽  
...  
Keyword(s):  
Splice Variant ◽  
Splice Variants ◽  
Immunoblot Analysis ◽  
Mouse Kidney ◽  
Wild Type ◽  
Rt Pcr ◽  
Outer Medulla ◽  
Bicarbonate Transporter ◽  
Outer Stripe ◽  
Acid Loading

Sodium-coupled bicarbonate transporters are critical for renal electrolyte transport. The electrogenic, sodium-coupled bicarbonate cotransporter, isoform 1 (NBCe1), encoded by the SLC4A4 geneencoded by the SLC4A4 gene has five multiple splice variants; the A splice variant, NBCe1-A, is the primary basolateral bicarbonate transporter in the proximal convoluted tubule. This study’s purpose was to determine if there is expression of additional NBCe1 splice variants in the mouse kidney, their cellular distribution, and their regulation by metabolic acidosis. In wild-type mice, an antibody reactive only to NBCe1-A showed basolateral immunolabel only in cortical proximal tubule (PT) segments, whereas an antibody reactive to all NBCe1 splice variants (pan-NBCe1) showed basolateral immunolabel in PT segments in both the cortex and outer medulla. In mice with NBCe1-A deletion, the pan-NBCe1 antibody showed basolateral PT immunolabel in both the renal cortex and outer stripe of the outer medulla, and immunoblot analysis showed expression of a ~121-kDa protein. RT-PCR of mRNA from NBCe1-A knockout mice directed at splice variant-specific regions showed expression of only NBCe1-B mRNA. In wild-type kidney, RT-PCR confirmed expression of mRNA for the NBCe1-B splice variant and absence of mRNA for the C, D, and E splice variants. Finally, exogenous acid loading increased expression in the proximal straight tubule in the outer stripe of the outer medulla. These studies demonstrate that the NBCe1-B splice variant is present in the PT, and its expression increases in response to exogenous acid loading, suggesting it participates in the PT contribution to acid-base homeostasis.

AQP3, p-AQP2, and AQP2 expression is reduced in polyuric rats with hypercalcemia: prevention by cAMP-PDE inhibitors

2002 ◽  
Vol 283 (6) ◽  
pp. F1313-F1325 ◽  
Author(s):  
Weidong Wang ◽  
Chunling Li ◽  
Tae-Hwan Kwon ◽  
Mark A. Knepper ◽  
Jørgen Frøkiær ◽  
...  
Keyword(s):  
Vitamin D ◽  
Collecting Duct ◽  
Urine Osmolality ◽  
Outer Medulla ◽  
Camp Phosphodiesterase ◽  
Aqp4 Expression ◽  
Pde Inhibitors ◽  
Aqp1 Expression ◽  
Outer Stripe ◽  
Inhibitor Treatment

The purpose of this study was to evaluate whether hypercalcemia is associated with downregulation of renal aquaporins (AQPs), including AQP1, AQP2, phosphorylated AQP2 (p-AQP2), AQP3, and AQP4, and if this is the case, to test whether cAMP-phosphodiesterase (PDE) inhibitor treatment can prevent AQP downregulation and prevent the development of polyuria. Vitamin D-induced hypercalcemia in rats was associated with increased urine output and reduced urine osmolality, consistent with previous findings (Levi M, Peterson L, and Berl T. Kidney Int 23: 489–497, 1983). Semiquantitative immunoblotting revealed a significant reduction in the abundance of inner medullary AQP2 (52 ± 6% of control levels), consistent with previous studies, and of AQP2, which is phosphorylated at the PKA phosphorylation consensus site serine 256 (p-AQP2; 36 ± 8%). Moreover, AQP3 abundance was also significantly decreased (45 ± 7 and 61 ± 6% of control levels in inner medulla and whole kidney, respectively). Consistent with this, immunohistochemistry demonstrated reduced AQP3 immunolabeling along the entire collecting duct. AQP4 expression was not reduced. Surprisingly, total kidney AQP1 abundance was also reduced (60 ± 6%). AQP1 expression was reduced in the cortex and outer stripe of the outer medulla (48 ± 7%; i.e., in proximal tubules). In contrast, AQP1 levels were not changed in the inner stripe of the outer medulla or in the inner medulla (i.e., descending thin limbs and vasa recta). Treatment with the cAMP-PDE inhibitors rolipram and milrinone in combination (inhibiting PDE IV and PDE III isoenzymes) at day 2 and onward completely prevented the hypercalcemia-induced downregulation of AQP2 and AQP3 (but not AQP1) and completely prevented the development of polyuria. In conclusion, AQP3, AQP2, and p-AQP2 are downregulated and are likely to play critical roles in the development of polyuria associated with vitamin D-induced hypercalcemia. Moreover, PDE inhibitor treatment significantly prevented the reduced expression of collecting duct AQPs and prevented the development of polyuria.

Temporal induction of clusterin in cisplatin nephrotoxicity.

1997 ◽  
Vol 8 (2) ◽  
pp. 302-305
Author(s):  
J R Silkensen ◽  
A Agarwal ◽  
K A Nath ◽  
J C Manivel ◽  
M E Rosenberg
Keyword(s):  
Serum Creatinine ◽  
Time Course ◽  
Tissue Injury ◽  
Kidney Tissue ◽  
Sprague Dawley ◽  
Outer Medulla ◽  
Creatinine Concentration ◽  
Cisplatin Nephrotoxicity ◽  
Tubular Necrosis ◽  
Outer Stripe

Clusterin is a ubiquitous glycoprotein induced in many organs, including the kidney, at times of tissue injury and/or remodeling. It is speculated in this study that clusterin preserves cell interactions that are otherwise perturbed by renal insults. The purpose of this study was to examine clusterin expression after cisplatin nephrotoxicity, a model characterized by a delayed time course of injury and a well-defined site of that injury (proximal tubule). Sprague-Dawley rats were treated with intravenous cisplatin (6 mg/kg) or vehicle. Serum creatinine concentrations were measured and kidneys harvested at 1, 2, and 5 days. Marked induction of clusterin mRNA was seen only at 5 days, a time when serum creatinine concentration was the highest. Histology of kidney tissue 5 days after cisplatin administration revealed marked tubular necrosis localized to the outer stripe of the outer medulla, a region rich in proximal tubules. Immunohistochemistry and in situ hybridization at 5 days demonstrated clusterin primarily in the inner stripe of the outer medulla. In conclusion, expression of clusterin follows renal injury with cisplatin at a time corresponding to the morphologic evidence of tubular necrosis and cell detachment; quite surprisingly, such expression occurs at a site distant from the primary injury.

Distinct cellular localization of mRNAs for three subtypes of prostaglandin E receptor in kidney

1994 ◽  
Vol 266 (5) ◽  
pp. F823-F828 ◽  
Author(s):  
Y. Sugimoto ◽  
T. Namba ◽  
R. Shigemoto ◽  
M. Negishi ◽  
A. Ichikawa ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Cellular Localization ◽  
Mouse Kidney ◽  
Prostaglandin E ◽  
Outer Medulla ◽  
Multiple Functions ◽  
Collecting Ducts ◽  
Distal Tubules ◽  
Stimulation Of

Distribution of the mRNAs for three subtypes of prostaglandin E (PGE) receptors in the mouse kidney was investigated by in situ hybridization. The mRNA for EP1 subtype, which is coupled to Ca2+ mobilization, was specifically localized to the collecting ducts from the cortex to the papilla. The mRNA for EP2 subtype, which is linked to stimulation of adenylate cyclase, was localized to the glomeruli. The mRNA for EP3 subtype, which is coupled to inhibition of adenylate cyclase, was located densely in the tubules in the outer medulla and in the distal tubules in the cortex. These results exhibit distinct cellular localization of three subtypes of PGE receptor in the kidney and suggest that PGE2 exerts multiple functions via these subtypes expressed in different segments of the nephron.

Increased apical targeting of renal ENaC subunits and decreased expression of 11βHSD2 in HgCl2-induced nephrotic syndrome in rats

2006 ◽  
Vol 290 (3) ◽  
pp. F674-F687 ◽  
Author(s):  
Soo Wan Kim ◽  
Sophie de Seigneux ◽  
Martin C. Sassen ◽  
JongUn Lee ◽  
Jin Kim ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Sodium Excretion ◽  
Collecting Duct ◽  
Urinary Sodium Excretion ◽  
Brown Norway ◽  
Apical Plasma Membrane ◽  
Control Group ◽  
Sodium Retention ◽  
Outer Medulla ◽  
Outer Stripe

Nephrotic syndrome is often accompanied by sodium retention and generalized edema. We hypothesize that dysregulation of the epithelial sodium channel (ENaC) and/or of sodium (co)transporters may be responsible for the increased sodium retention associated with HgCl2-induced nephropathy. In addition, we examined the hypothesis that the expression of type 2 11β-hydroxysteroid dehydrogenase (11βHSD2) is reduced, contributing to the enhanced mineralocorticoid activity. Membranous nephropathy was induced in Brown Norway rats by repeated injections of HgCl2 (1 mg/kg sc), whereas the control group received only vehicle. After 13 days of treatment, the abundance of ENaC subunits, sodium (co)transporters, and 11βHSD2 in the kidney was examined by immunoblotting and immunohistochemistry. HgCl2 treatment induced marked proteinuria, hypoalbuminemia, decreased urinary sodium excretion, and ascites. The protein abundance of α-ENaC was increased in the cortex/outer stripe of outer medulla (OSOM) and inner stripe of the outer medulla (ISOM). The protein abundances of β-ENaC and γ-ENaC were decreased in the cortex/OSOM while increased in the ISOM. Immunoperoxidase microscopy demonstrated increased targeting of ENaC subunits to the apical plasma membrane in the distal convoluted tubule, connecting tubule, and cortical and medullary collecting duct segments. Moreover, 11βHSD2 abundance was decreased in cortex/OSOM and ISOM. The protein abundances of type 3 Na/H exchanger (NHE3), Na-K-2Cl cotransporter (NKCC2), and thiazide-sensitive Na-Cl cotransporter (NCC) were decreased. Moreover, the abundance of the α-1 subunit of the Na-K-ATPase was decreased in the cortex/OSOM and ISOM but remained unchanged in the inner medulla. These results suggest that increased apical targeting of ENaC subunits combined with diminished abundance of 11βHSD2 may contribute to sodium retention associated with HgCl2-induced nephrotic syndrome. The decreased abundance of NHE3, NKCC2, NCC, and Na-K-ATPase may play a compensatory role in promoting sodium excretion.

Freezing point depression of rat kidney slices during water diuresis and antidiuresis

1960 ◽  
Vol 199 (5) ◽  
pp. 915-918 ◽  
Author(s):  
George A. Bray
Keyword(s):  
Pressure Gradient ◽  
Osmotic Pressure ◽  
Freezing Point ◽  
Rat Kidney ◽  
Freezing Point Depression ◽  
Water Diuresis ◽  
Outer Medulla ◽  
Kidney Slices ◽  
Outer Stripe ◽  
Osmotic Pressure Gradient

The freezing point depression of slices of rat kidney removed during water diuresis or antidiuresis has been investigated with a microcryoscopic method. The osmotic pressure gradient in the inner medulla first demonstrated by Wirz has been confirmed. The inner medulla was found to be hypertonic to plasma during water diuresis. Hypotonic tubules were present throughout the cortex and outer stripe of the outer medulla.

Distribution and oligomeric association of splice forms of Na+-K+-ATPase regulatory γ-subunit in rat kidney

2002 ◽  
Vol 282 (3) ◽  
pp. F393-F407 ◽  
Author(s):  
Elena Arystarkhova ◽  
Randall K. Wetzel ◽  
Kathleen J. Sweadner
Keyword(s):  
Splice Variants ◽  
Rat Kidney ◽  
Thick Ascending Limb ◽  
Outer Medulla ◽  
Α Subunit ◽  
Proximal Tubule Cells ◽  
Γ Subunit ◽  
Outer Stripe ◽  
A Minor ◽  
Splice Forms

Renal Na+-K+-ATPase is associated with the γ-subunit (FXYD2), a single-span membrane protein that modifies ATPase properties. There are two splice variants with different amino termini, γa and γb. Both were found in the inner stripe of the outer medulla in the thick ascending limb. Coimmunoprecipitation with each other and the α-subunit indicated that they were associated in macromolecular complexes. Association was controlled by ligands that affect Na+-K+-ATPase conformation. In the cortex, the proportion of the γb-subunit was markedly lower, and the γa-subunit predominated in isolated proximal tubule cells. By immunofluorescence, the γb-subunit was detected in the superficial cortex only in the distal convoluted tubule and connecting tubule, which are rich in Na+-K+-ATPase but comprise a minor fraction of cortex mass. In the outer stripe of the outer medulla and for a short distance in the deep cortex, the thick ascending limb predominantly expressed the γb-subunit. Because different mechanisms maintain and regulate Na+ homeostasis in different nephron segments, the splice forms of the γ-subunit may have evolved to control the renal Na+ pump through pump properties, gene expression, or both.

Renal glutaminase I distribution and ammonia excretion in the rat

1960 ◽  
Vol 198 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Marc B. Weiss ◽  
James B. Longley
Keyword(s):  
Ammonia Excretion ◽  
Lower Activity ◽  
Outer Medulla ◽  
Outer Stripe ◽  
Collecting Tubules ◽  
Separated Zones

Glutaminase I assays were made on carefully separated zones of kidneys from normal and acidotic rats. Highest activity in normal rats was found in the inner stripe of the outer medulla, next lower activity in the inner medulla (papilla), still lower activity in the cortex, and lowest activity in the outer stripe of the outer medulla. Induced acidosis produced a physiologically significant increase only in the activity of the cortex. Results are discussed in relation to previous conflicting reports. It is concluded that although urinary ammonia may be excreted by the papillary collecting tubules, its release is unlikely to result from glutaminase I activity at that site.

Cloning, embryonic expression, and alternative splicing of a murine kidney-specific Na-K-Cl cotransporter

1995 ◽  
Vol 269 (3) ◽  
pp. F405-F418 ◽  
Author(s):  
P. Igarashi ◽  
G. B. Vanden Heuvel ◽  
J. A. Payne ◽  
B. Forbush
Keyword(s):  
In Situ Hybridization ◽  
Alternative Splicing ◽  
Protein Kinase ◽  
Thick Ascending Limb ◽  
Outer Medulla ◽  
Donor And Acceptor ◽  
Outer Stripe ◽  
Dependent Protein ◽  
Isoform B

A full-length cDNA encoding the murine renal Na-K-Cl cotransporter (NKCC2) was cloned using library screening and anchored polymerase chain reaction. The deduced protein sequence contained 1,095 amino acids and was 93.5% identical to rabbit NKCC2 and 97.6% identical to rat BSC1. Two potential sites of phosphorylation by adenosine 3',5'-cyclic monophosphate-dependent protein kinase and seven potential sites of phosphorylation by protein kinase C, which were previously identified in the rabbit and rat sequences, were phylogenetically conserved in the mouse. The expression of NKCC2 in the mouse was examined with Northern blot analysis and in situ hybridization. Expression of NKCC2 was kidney specific in both adult and embryonic mice. In the developing metanephros, NKCC2 was induced at 14.5 days post coitus and was expressed in distal limbs of immature loops of Henle but was absent from the ureteric bud, S-shaped bodies, and earlier nephrogenic structures. Similar to the rabbit, isoforms of NKCC2 that differed in the sequence of a 96-bp segment were identified in the mouse. In situ hybridization revealed that the isoforms exhibited different patterns of expression in the mature thick ascending limb of the loop of Henle as follows: isoform F was most highly expressed in the inner stripe of outer medulla, isoform A was most highly expressed in the outer stripe of the outer medulla, and isoform B was most highly expressed in the cortical thick ascending limb. To verify that the isoforms were generated by alternative splicing of mutually exclusive cassette exons, genomic clones encoding murine NKCC2 were characterized. Cassette exons were identified that corresponded to each of the three isoforms and were flanked by consensus splice donor and acceptor sequences.

Sign in / Sign up

Export Citation Format

Share Document