Office Space Bacterial Abundance and Diversity in Three Metropolitan Areas

Viruses are ubiquitous in natural systems. By influencing bacterial abundance (BA) and community structure through lysis-lysogenic conversion, viruses are involved in various ecological processes. In agricultural management, nitrogen addition and irrigation should be considered as important factors that can modify soil viral dynamics but have been ignored. In our study, short-term dynamics of autochthonous soil viral and bacterial abundance and diversity after irrigation and urea application were examined in a long-term experimental paddy field. Urea addition delayed the emergence of peak viral abundance for three days, suggesting that viruses are sensitive to N addition. Under short-term eutrophic conditions through urea application, viruses undertake a lysogenic-biased strategy. Moreover, nitrogen-fixing bacteria were most likely specifically lysed in urea-treated soil, which suggests that soil viruses block N accumulation by killing nitrogen-fixing bacteria. To the best of our knowledge, this study is the first to investigate dynamic changes in autochthonous viruses in paddy fields.

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Comparison of Innovative Molecular Approaches and Standard Spore Assays for Assessment of Surface Cleanliness

Applied and Environmental Microbiology ◽

10.1128/aem.00192-11 ◽

2011 ◽

Vol 77 (15) ◽

pp. 5438-5444 ◽

Cited By ~ 24

Author(s):

Moogega Cooper ◽

Myron T. La Duc ◽

Alexander Probst ◽

Parag Vaishampayan ◽

Christina Stam ◽

...

Keyword(s):

Dna Microarray ◽

Dna Microarrays ◽

Bacterial Abundance ◽

State Of The Art ◽

Molecular Techniques ◽

Molecular Approaches ◽

Abundance And Diversity ◽

Classical Culture ◽

Genetic Signatures ◽

Sample Set

ABSTRACTA bacterial spore assay and a molecular DNA microarray method were compared for their ability to assess relative cleanliness in the context of bacterial abundance and diversity on spacecraft surfaces. Colony counts derived from the NASA standard spore assay were extremely low for spacecraft surfaces. However, the PhyloChip generation 3 (G3) DNA microarray resolved the genetic signatures of a highly diverse suite of microorganisms in the very same sample set. Samples completely devoid of cultivable spores were shown to harbor the DNA of more than 100 distinct microbial phylotypes. Furthermore, samples with higher numbers of cultivable spores did not necessarily give rise to a greater microbial diversity upon analysis with the DNA microarray. The findings of this study clearly demonstrated that there is not a statistically significant correlation between the cultivable spore counts obtained from a sample and the degree of bacterial diversity present. Based on these results, it can be stated that validated state-of-the-art molecular techniques, such as DNA microarrays, can be utilized in parallel with classical culture-based methods to further describe the cleanliness of spacecraft surfaces.

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Bacterial abundance and diversity in pond water supplied with different feeds

Scientific Reports ◽

10.1038/srep35232 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 30

Author(s):

Ya Qin ◽

Jie Hou ◽

Ming Deng ◽

Quansheng Liu ◽

Chongwei Wu ◽

...

Keyword(s):

Bacterial Abundance ◽

Pond Water ◽

Abundance And Diversity

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Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil

Journal of Microbiological Methods ◽

10.1016/j.mimet.2008.09.020 ◽

2009 ◽

Vol 76 (2) ◽

pp. 120-127 ◽

Cited By ~ 17

Author(s):

Nadia Khammar ◽

Gaëtan Martin ◽

Katia Ferro ◽

Daniel Job ◽

Michel Aragno ◽

...

Keyword(s):

Molecular Marker ◽

Bacterial Abundance ◽

Abundance And Diversity ◽

Diversity Structure

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Bacterial abundance and diversity in the Barbados Trench determined by phospholipid analysis

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.1996.tb00201.x ◽

1996 ◽

Vol 19 (2) ◽

pp. 83-93 ◽

Cited By ~ 47

Author(s):

J. Guezennec ◽

A. Fiala-Medioni

Keyword(s):

Bacterial Abundance ◽

Phospholipid Analysis ◽

Abundance And Diversity

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Pesticides Decrease Bacterial Diversity and Abundance of Irrigated Rice Fields

Microorganisms ◽

10.3390/microorganisms8030318 ◽

2020 ◽

Vol 8 (3) ◽

pp. 318 ◽

Cited By ~ 1

Author(s):

Michael Onwona-Kwakye ◽

Kimberly Plants-Paris ◽

Kadiatou Keita ◽

Jessica Lee ◽

Paul J. Van den Brink ◽

...

Keyword(s):

Bacterial Diversity ◽

Bacterial Abundance ◽

Management Strategies ◽

Diversity Indices ◽

Rice Fields ◽

Irrigated Rice ◽

Operational Taxonomic Units ◽

Soil Ecosystems ◽

Abundance And Diversity ◽

Aerobic And Anaerobic

Bacteria play an important role in soil ecosystems and their activities are crucial in nutrient composition and recycling. Pesticides are extensively used in agriculture to control pests and improve yield. However, increased use of pesticides on agricultural lands results in soil contamination, which could have adverse effect on its bacterial communities. Here, we investigated the effect of pesticides commonly used on irrigated rice fields on bacterial abundance and diversity. Irrigated soil samples collected from unexposed, pesticide-exposed, and residual exposure areas were cultured under aerobic and anaerobic conditions. DNA was extracted and analysed by 16S rRNA sequencing. The results showed overall decrease in bacterial abundance and diversity in areas exposed to pesticides. Operational taxonomic units of the genera Enterobacter, Aeromonas, Comamonas, Stenotrophomonas, Bordetella, and Staphylococcus decreased in areas exposed to pesticides. Conversely, Domibacillus, Acinetobacter, Pseudomonas, and Bacillus increased in abundance in pesticide-exposed areas. Simpson and Shannon diversity indices and canonical correspondence analysis demonstrated a decrease in bacterial diversity and composition in areas exposed to pesticides. These results suggest bacteria genera unaffected by pesticides that could be further evaluated to identify species for bioremediation. Moreover, there is a need for alternative ways of improving agricultural productivity and to educate farmers to adopt innovative integrated pest management strategies to reduce deleterious impacts of pesticides on soil ecosystems.

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Chetoui Olive Cultivar Rhizosphere: Potential Reservoir for Exoenzymes and Exopolysaccharides Producing Bacteria

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.4.32 ◽

2020 ◽

Vol 14 (4) ◽

pp. 2569-2575

Author(s):

Ben Amar Cheba ◽

H.M.A. Abdelzaher

Keyword(s):

Bacterial Abundance ◽

Geographical Origin ◽

Enteric Bacteria ◽

Biotechnological Applications ◽

Olive Cultivar ◽

Potential Reservoir ◽

Olive Cultivars ◽

Preliminary Study ◽

Abundance And Diversity ◽

Bacterial Groups

Rhizospheric soils from cultivated olive (Olea europaea) trees of Chemlali, Chetoui, Quaissi, and Djalat cultivars were assessed for their bacterial abundance and diversity and were further screened for production of exopolysaccharides and exoenzymes (cellulase, chitinase, amylase, protease, lipase, and peroxidase). The results of the present study indicate that Chetoui cultivar revealed higher diversity, followed by Chemlali > Quaissi > Djalat, wherein, bacilli, enteric bacteria, and pseudomonads were abundantly present as specific bacterial groups associated with the Chetoui rhizosphere. Moreover, the exopolysaccharide (EPS)-producing bacteria of Chetoui cultivar (68.4%) presented the highest efficiency, followed by Djalat (23.5%) > Chemlali (7 %) > Quaissi (1%). These results revealed that the Chetoui cultivar presented highest enzyme activities, followed by Chemlali > Djalat > Quaissi, with a distinct abundance of peroxidase- and chitinase-producing bacteria, which may play a pivotal role in adapting olives to the environmental stresses. From this preliminary study, we confirmed that olive rhizosphere microbial diversity is essentially driven by the geographical origin and genotype of olive cultivars. Furthermore, we recommended the Chetoui olive cultivar rhizosphere as a potential reservoir for exoenzyme- and EPS-producing bacteria useful for future biotechnological applications.

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