scholarly journals Exploiting Genomic Knowledge in Optimising Molecular Breeding Programmes: Algorithms from Evolutionary Computing

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48862 ◽  
Author(s):  
Steve O'Hagan ◽  
Joshua Knowles ◽  
Douglas B. Kell
2017 ◽  
Vol 45 (2) ◽  
pp. 77 ◽  
Author(s):  
Mainaak Mukhopdhayay, Akan Das Tapan Kumar Mondal

<p>Tea is an important plantation crop of India, which generates huge employment opportunities in rural and hilly backward places.<br />Being a woody perennial crop with an economic life span of more than 60 years, it also has a role in sustaining the ecosystem.<br />Because of its long gestation period, as observed from conventional breeding, alternative methods such as molecular breeding is<br />highly relevant, which is rather limited in tea breeding programmes. Therefore, adoption of biotechnological approaches is a<br />better option to shorten the breeding cycle of tea. Recent developments from the biotechnological research works on tea and<br />related species are summarized in the review.</p>


Author(s):  
Inès Yacoubi ◽  
Emna Khanfir ◽  
Karama Hamdi ◽  
Faïçal Brini

Abstract This chapter provides an outline of the mechanisms of wheat salinity tolerance and discusses the challenges of several breeding programmes that are in progress with regard to durum (Triticum turgidum subsp. durum) and bread wheats using the integration of trait-based and molecular selection for delivering improved wheat varieties adapted to saline conditions.


2017 ◽  
Vol 9 (2) ◽  
pp. 1119-1122
Author(s):  
Vikash Kumar ◽  
Pawan Kumar ◽  
Tirthartha Chattopadhyay

As most of the molecular markers in crop molecular breeding programmes are successful based on polymerase chain reaction (PCR), the isolated genomic DNA must be suitable for the same. Though PCR is a robust method and in most of the cases requires only a minute amount of genomic DNA as template, removal of potential PCR-inhibitory factors is quite important. The present work reports the optimization of a rapid genomic DNA isolation method, suitable for PCR-based genotyping of plants. As very minute amount of the genomic DNA isolated in this rapid method was found to be sufficient for PCR, a researcher is capable to go for several hundred independent PCR from single isolation. The method was validated in 4 different crops (wheat, tomato, brinjal and cauliflower) using different PCR-based molecular markers. In case of wheat, genomic DNA isolated in this method was found to be suitable PCR using the specific marker for the detection of the Lr34 gene. For tomato, genomic DNA isolated in this method was successfully used with the molecular markers for the detection of resistance alleles for yellow leaf curl disease and root knot disease. In case of brinjal, the isolated genomic DNA was found to be suitable for simple sequence repeat (SSR) marker assay. In a similar way, genomic DNA isolated in this method from cauliflower leaves was observed to be suitable for amplifying a gene of ~1.5 kb length. Thus, this method will be quite helpful to expedite marker assisted selection of plants in plant molecular breeding programmes.


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.


2006 ◽  
Vol 54 (3) ◽  
pp. 343-350 ◽  
Author(s):  
C. F. H. Longin ◽  
H. F. Utz ◽  
A. E. Melchinger ◽  
J.C. Reif

The optimum allocation of breeding resources is crucial for the efficiency of breeding programmes. The objectives were to (i) compare selection gain ΔGk for finite and infinite sample sizes, (ii) compare ΔGk and the probability of identifying superior hybrids (Pk), and (iii) determine the optimum allocation of the number of hybrids and test locations in hybrid maize breeding using doubled haploids. Infinite compared to finite sample sizes led to almost identical optimum allocation of test resources, but to an inflation of ΔGk. This inflation decreased as the budget and the number of finally selected hybrids increased. A reasonable Pk was reached for hybrids belonging to the q = 1% best of the population. The optimum allocations for Pk(q) and ΔGkwere similar, indicating that Pk(q) is promising for optimizing breeding programmes.


2006 ◽  
Vol 54 (3) ◽  
pp. 351-358 ◽  
Author(s):  
P. Pepó

Plant regeneration via tissue culture is becoming increasingly more common in monocots such as maize (Zea mays L.). Pollen (gametophytic) selection for resistance to aflatoxin in maize can greatly facilitate recurrent selection and the screening of germplasm for resistance at much less cost and in a shorter time than field testing. In vivo and in vitro techniques have been integrated in maize breeding programmes to obtain desirable agronomic attributes, enhance the genes responsible for them and speed up the breeding process. The efficiency of anther and tissue cultures in maize and wheat has reached the stage where they can be used in breeding programmes to some extent and many new cultivars produced by genetic manipulation have now reached the market.


2019 ◽  
Vol 51 (6) ◽  
Author(s):  
Zhonghua Sheng ◽  
Sajid Fiaz ◽  
Qianlong Li ◽  
Wei Chen ◽  
Xiangjin Wei ◽  
...  

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.


2014 ◽  
Vol 48 (6) ◽  
pp. 589-607
Author(s):  
Dai Silan ◽  
Huang He ◽  
Fu Jianxin ◽  
Hong Yan

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