Systemic Analysis of Gene Expression Profiles Identifies ErbB3 as a Potential Drug Target in Pediatric Alveolar Rhabdomyosarcoma

AbstractSepsis is a life-threatening condition associate with significant morbidity and mortality, but limited treatment. Mitochondria are recently recognized to be related to the pathophysiology of sepsis, and mitochondria could serve as a potential drug target. In our study, we aim to identify biological functions and pathways of mitochondria during the processes of sepsis by using a bioinformatics method to elucidate their potential pathogenesis. The gene expression profiles of the GSE167914 dataset were originally created by using the Nanostring nCounter Elements™ TagSet preselected for mitochondrial biogenesis and function panel. The biological pathways were analyzed by the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG), Gene Ontology (GO), and Reactome enrichment. KEGG and GO results showed the Neurodegeneration pathways such as Huntington and Parkinson pathways were mostly affected in the development of sepsis. Moreover, we identified several mitochondrial genes including TOMM40, TOMM20, TIMM22, TIMM10, TIMM17A, TIMM9, TIMM44 were involved in the regulation of protein translocation into mitochondria. Further, we predicted several regulators that had the ability to affect the mitochondria during sepsis by L1000fwd analysis. Thus, this study provides further insights into the mechanism of mitochondrial function during sepsis.

Download Full-text

Gene Expression Profiles of Peripheral Blood Monocytes in Osteoarthritis and Analysis of Differentially Expressed Genes

BioMed Research International ◽

10.1155/2019/4291689 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12

Author(s):

Ting Shi ◽

Xiongjie Shen ◽

Ge Gao

Keyword(s):

Gene Expression ◽

Signaling Pathway ◽

Peripheral Blood ◽

Expression Profiles ◽

Osteoclast Differentiation ◽

Gene Expression Profiles ◽

Mapk Signaling ◽

Molecular Pathogenesis ◽

Mapk Signaling Pathway ◽

Potential Drug

Background. There is little understanding of the molecular processes involved in the pathogenesis of osteoarthritis, limiting early diagnosis and effective treatment of OA. Use of genechips can provide insights into the molecular pathogenesis of diseases. In this study, determination of gene expression profiles of osteoarthritis peripheral blood mononuclear cells will allow exploration of the molecular pathogenesis of OA and find out more candidate biomarkers and potential drug targets of OA. Result. A total of 1231 DEGs were screened out including 791 upregulated DEGs and 440 downregulated DEGs. The most significant upregulated DEG was RPL38, which may inhibit chondrocyte differentiation and synthesis of the extracellular matrix. PIK3CA, PIK3CB, PIK3CD, PIK3R1, MAPK14, IL1A, JUND, FOSL2, and PPP3CA were the gene symbols of the osteoclast differentiation pathway which was the most significant pathway enriched by DEGs. However, the MAPK signaling pathway occupied the core position of all the pathways which can regulate apoptosis, cell cycle, wnt signaling pathway, p53 signaling pathway, and phosphatidylinositol signaling system. Furthermore, PI3Ks may regulate IL1A, JUND, FOSL2 and PPP3CA through the MAPK signaling pathway. Conclusion. These identified DEGs and pathways may be novel biomarkers to monitor the changes of OA and can be a potential drug target for the treatment of OA.

Download Full-text