scholarly journals Identification and Expression Analysis of microRNAs at the Grain Filling Stage in Rice(Oryza sativa L.)via Deep Sequencing

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57863 ◽  
Author(s):  
Rong Yi ◽  
Zhixuan Zhu ◽  
Jihong Hu ◽  
Qian Qian ◽  
Jincheng Dai ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Expression Analysis ◽  
Deep Sequencing ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Filling Stage ◽  
Grain Filling Stage

Relationship between leaf photosynthetic function at grain filling stage and yield in super high-yielding hybrid rice (Oryza sativa. L)

2002 ◽  
Vol 45 (6) ◽  
pp. 637-646 ◽  
Author(s):  
Huqu Zhai ◽  
Shuqing Cao ◽  
Jianmin Wan ◽  
Rongxian Zhang ◽  
Wei Lu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Hybrid Rice ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Filling Stage ◽  
Grain Filling Stage

scholarly journals Changes In Biochemical Components Of Rice Following Naa Application

2014 ◽  
Vol 40 (2) ◽  
pp. 173-178
Author(s):  
Nargis Jahan ◽  
AMM Golam Adam
Keyword(s):  
Chlorophyll A ◽  
Energy Content ◽  
Oryza Sativa L ◽  
Foliar Spray ◽  
Grain Filling ◽  
Naphthalene Acetic Acid ◽  
Flowering Stage ◽  
Filling Stage ◽  
Biochemical Components ◽  
Grain Filling Stage

An investigation on the effect of Naphthalene acetic acid (NAA) on the changes of biochemical components of two varieties of rice (Oryza sativa L.), BRR1 dhan 29 and BRRI dhan 50 was carried out. Two concentrations of NAA, 100 and 200 ppm were used as foliar spray. In BRRI dhan 29, significant increases in chlorophyll a and chlorophyll b contents of leaf were observed due to both the treatments at the flowering stage, whereas, carotenoids at the grain filling stage only. But in BRRI dhan 50, the total leaf pigments decreased at the flowering and grain filling stage, but was significant in case of chlorophyll a at the flowering stage. Significant increases in protein content of leaf were recorded at the tillering and flowering stage of BRRI dhan 29 following 100 ppm NAA treatment. In BRRI dhan 50, the only significant increase was recorded at grain filling stage due to 200 ppm NAA treatment. In both the varieties carbohydrates, protein, fat, and energy content of grain increased due to both the treatments and the maximum increase in all the cases was recorded due to 100 ppm NAA treatment. Ash and moisture content of grain reduced following both the treatments in both the varieties, the maximum reduction was due to 100 ppm NAA treatment Asiat. Soc. Bangladesh, Sci. 40(2): 173-178, December 2014

scholarly journals Physiological and Transcriptome Analyses of Early Leaf Senescence for ospls1 Mutant Rice (Oryza sativa L.) during the Grain-Filling Stage

2019 ◽  
Vol 20 (5) ◽  
pp. 1098 ◽  
Author(s):  
Zhaowei Li ◽  
Xinfeng Pan ◽  
Xiaodong Guo ◽  
Kai Fan ◽  
Wenxiong Lin
Keyword(s):  
Oryza Sativa ◽  
Signaling Pathways ◽  
Leaf Senescence ◽  
Grain Filling ◽  
Differentially Expressed ◽  
Hormone Signaling ◽  
Rna Seq ◽  
Filling Stage ◽  
Grain Filling Stage ◽  
Early Leaf Senescence

Early leaf senescence is an important agronomic trait that affects crop yield and quality. To understand the molecular mechanism of early leaf senescence, Oryza sativa premature leaf senescence 1 (ospls1) mutant rice with a deletion of OsVHA-A and its wild type were employed in this study. The genotype-dependent differences in photosynthetic indexes, senescence-related physiological parameters, and yield characters were investigated during the grain-filling stage. Moreover, RNA sequencing (RNA-seq) was performed to determine the genotype differences in transcriptome during the grain-filling stage. Results showed that the ospls1 mutant underwent significant decreases in the maximal quantum yield of photosystem II (PSII) photochemistry (Fv/Fm), net photosynthesis rate (Pn), and soluble sugar and protein, followed by the decreases in OsVHA-A transcript and vacuolar H+-ATPase activity. Finally, yield traits were severely suppressed in the ospls1 mutant. RNA-seq results showed that 4827 differentially expressed genes (DEGs) were identified in ospls1 mutant between 0 day and 14 days, and the pathways of biosynthesis of secondary metabolites, carbon fixation in photosynthetic organisms, and photosynthesis were downregulated in the senescing leaves of ospls1 mutant during the grain-filling stage. In addition, 81 differentially expressed TFs were identified to be involved in leaf senescence. Eleven DEGs related to hormone signaling pathways were significantly enriched in auxin, cytokinins, brassinosteroids, and abscisic acid pathways, indicating that hormone signaling pathways participated in leaf senescence. Some antioxidative and carbohydrate metabolism-related genes were detected to be differentially expressed in the senescing leaves of ospls1 mutant, suggesting that these genes probably play response and regulatory roles in leaf senescence.

Effect of Temperature during Grain Filling Stage on Endosperm Structure and Apperance Quality of Aromatic Rice

2012 ◽  
Vol 460 ◽  
pp. 286-289 ◽  
Author(s):  
Zhen Jiang Xu ◽  
Li Zhong Xiao ◽  
Hong Liu ◽  
Yong Hao Ren ◽  
Zhi Lin Li
Keyword(s):  
Grain Filling ◽  
Effect Of Temperature ◽  
Rice Grain ◽  
Aromatic Rice ◽  
Starch Granules ◽  
Filling Stage ◽  
Appearance Quality ◽  
Endosperm Structure ◽  
Grain Filling Stage

Based on the experiment of two inbred aromatic rice varieties and one hybrid aromatic rice line treated under four different temperatures( daymean temperature 21°C, 23°C, 26°C and 30°C respectively) during grain filling stage in phytotrons, the shape and arrangement of endosperm starch granules in rice grain transection were observed by scanning electron microscope and the related characteristics of rice grain qualities of chalky percent and chalkiness were analyzed at the same time. The results showed that under the lower temperature( daymean temperature21°C and 23°C), many large compound starch granules with clear angulars packed together regularly without significant natural gaps bewteen starch granules in the tansectional endosperm. However, with the increase of temperature, starch granules in the transectional endosperm were changed from regularly shaped and closely and orderly arranged to various shaped and chaoticly arranged with obvious natural gaps between starch granules, which was closely consistent with the poorer appearance quality under the higher temperature, which indicated that the endosperm structure is closely related with appearance quality of aromatic rice.

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