scholarly journals Copy Number Change of the NDM-1 Sequence in a Multidrug-Resistant Klebsiella pneumoniae Clinical Isolate

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62774 ◽  
Author(s):  
Tzu-Wen Huang ◽  
Te-Li Chen ◽  
Ying-Tsong Chen ◽  
Tsai-Ling Lauderdale ◽  
Tsai-Lien Liao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Copy Number ◽  
Copy Number Change ◽  
Multidrug Resistant

scholarly journals “Clicking” an Ionic Liquid to a Potent Antimicrobial Peptide: On the Route towards Improved Stability

2020 ◽  
Vol 21 (17) ◽  
pp. 6174
Author(s):  
Ana Gomes ◽  
Lucinda J. Bessa ◽  
Patrícia Correia ◽  
Iva Fernandes ◽  
Ricardo Ferraz ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Peptide ◽  
Clinical Isolate ◽  
Bioactive Peptides ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Improved Stability

A covalent conjugate between an antibacterial ionic liquid and an antimicrobial peptide was produced via “click” chemistry, and found to retain the parent peptide’s activity against multidrug-resistant clinical isolates of Gram-negative bacteria, and antibiofilm action on a resistant clinical isolate of Klebsiella pneumoniae, while exhibiting much improved stability towards tyrosinase-mediated modifications. This unprecedented communication is a prelude for the promise held by ionic liquids -based approaches as tools to improve the action of bioactive peptides.

scholarly journals Identification of a Plasmid Encoding SHV-12, TEM-1, and a Variant of IMP-2 Metallo-β-Lactamase, IMP-8, from a Clinical Isolate of Klebsiella pneumoniae

2001 ◽  
Vol 45 (8) ◽  
pp. 2368-2371 ◽  
Author(s):  
Jing-Jou Yan ◽  
Wen-Chien Ko ◽  
Jiunn-Jong Wu
Keyword(s):  
Amino Acid ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Gene Cassette ◽  
Multidrug Resistant

ABSTRACT A multidrug-resistant plasmid encoding TEM-1, SHV-12, and a variant of IMP-2 metallo-β-lactamase, designated IMP-8, was identified from a clinical isolate of Klebsiella pneumoniae. There are four nucleotide differences between bla IMP-2 andbla IMP-8, resulting in two amino acid differences. bla IMP-8 was also found to be carried by an integron-borne gene cassette similar to thebla IMP-2 cassette.

scholarly journals RmtF, a New Member of the Aminoglycoside Resistance 16S rRNA N7 G1405 Methyltransferase Family

2012 ◽  
Vol 56 (7) ◽  
pp. 3960-3962 ◽  
Author(s):  
Marc Galimand ◽  
Patrice Courvalin ◽  
Thierry Lambert
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Multidrug Resistant ◽  
Aminoglycoside Resistance ◽  
Content Type

ABSTRACTMultidrug-resistant clinical isolateKlebsiella pneumoniaeBM4686 was highly resistant to 4,6-disubstituted 2-deoxystreptamines and to fortimicin. Resistance was due to the presence, on the 40-kb non-self-transferable plasmid pIP849, of thermtFgene which was cotranscribed with the upstreamaac(6′)-Ibgene. The deduced RmtF protein had 25 to 46% identity with members of the N7 G1405 family of aminoglycoside resistance 16S rRNA methyltransferases.

scholarly journals Draft Genome Assemblies of Clinical Isolates of Klebsiella pneumoniae V9011662 and Enterobacter hormaechei Entb306

2019 ◽  
Vol 8 (15) ◽  
Author(s):  
Lucas B. Harrison ◽  
Anna Selmecki ◽  
Nancy D. Hanson
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Content Type ◽  
Enterobacter Hormaechei ◽  
Genome Assemblies

Enterobacter hormaechei and Klebsiella pneumoniae are pathogenic Enterobacteriaceae that have been associated with the spread of antibiotic resistance. Here, we report draft genome assemblies of an Enterobacter hormaechei clinical isolate and a multidrug-resistant clinical isolate of Klebsiella pneumoniae.

scholarly journals Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

2021 ◽  
Author(s):  
Weverton de Oliveira Alves ◽  
Alexsandra Maria Lima Scavuzzi ◽  
Elizabeth Maria Bispo Beltrão ◽  
Érica Maria de Oliveira ◽  
Crhisllane Rafaele dos Santos Vasconcelos ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Plasmid Dna ◽  
Horizontal Transfer ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Pcr Analysis ◽  
First Report

Abstract To characterize phenotypically and genotypically an isolate of Multidrug Resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated by using PCR and sequencing the amplicons and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variant blaNDM-7 associated with blaKPC-2 and the cps, wabG, fimH, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of blaCTX-M15, aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the blaNDM-7 gene in Brazil and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.

scholarly journals Emergence of Salmonella Genomic Island 1 Variant SGI1-C in a Multidrug-Resistant Clinical Isolate of Klebsiella pneumoniae ST485 from Egypt

2020 ◽  
Vol 64 (9) ◽  
Author(s):  
Ahmed M. Soliman ◽  
Hazem Ramadan ◽  
Eslam Ghazy ◽  
Liansheng Yu ◽  
Junzo Hisatsune ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Genomic Island ◽  
Multidrug Resistant

scholarly journals Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Alasdair T. M. Hubbard ◽  
Jenifer Mason ◽  
Paul Roberts ◽  
Christopher M. Parry ◽  
Caroline Corless ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Gene Amplification ◽  
Copy Number ◽  
Resistance Mechanism ◽  
Genomic Data ◽  
Fitness Cost ◽  
Tandem Array ◽  
Resistant Isolate

Abstract A phenotype of Escherichia coli and Klebsiella pneumoniae, resistant to piperacillin/tazobactam (TZP) but susceptible to carbapenems and 3rd generation cephalosporins, has emerged. The resistance mechanism associated with this phenotype has been identified as hyperproduction of the β-lactamase TEM. However, the mechanism of hyperproduction due to gene amplification is not well understood. Here, we report a mechanism of gene amplification due to a translocatable unit (TU) excising from an IS26-flanked pseudo-compound transposon, PTn6762, which harbours blaTEM-1B. The TU re-inserts into the chromosome adjacent to IS26 and forms a tandem array of TUs, which increases the copy number of blaTEM-1B, leading to TEM-1B hyperproduction and TZP resistance. Despite a significant increase in blaTEM-1B copy number, the TZP-resistant isolate does not incur a fitness cost compared to the TZP-susceptible ancestor. This mechanism of amplification of blaTEM-1B is an important consideration when using genomic data to predict susceptibility to TZP.

scholarly journals Biochemical Characterization of VIM-39, a VIM-1-Like Metallo-β-Lactamase Variant from a Multidrug-Resistant Klebsiella pneumoniae Isolate from Greece

2015 ◽  
Vol 59 (12) ◽  
pp. 7811-7814 ◽  
Author(s):  
Costas C. Papagiannitsis ◽  
Simona Pollini ◽  
Filomena De Luca ◽  
Gian Maria Rossolini ◽  
Jean-Denis Docquier ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Biochemical Characterization ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Turnover Rates ◽  
Content Type

ABSTRACTVIM-39, a VIM-1-like metallo-β-lactamase variant (VIM-1 Thr33Ala His224Leu) was identified in a clinical isolate ofKlebsiella pneumoniaebelonging to sequence type 147. VIM-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did VIM-1 and VIM-26 (a VIM-1 variant with the His224Leu substitution) because of higher turnover rates.

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