scholarly journals Draft Genome Assemblies of Clinical Isolates of Klebsiella pneumoniae V9011662 and Enterobacter hormaechei Entb306

2019 ◽  
Vol 8 (15) ◽  
Author(s):  
Lucas B. Harrison ◽  
Anna Selmecki ◽  
Nancy D. Hanson
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Content Type ◽  
Enterobacter Hormaechei ◽  
Genome Assemblies

Enterobacter hormaechei and Klebsiella pneumoniae are pathogenic Enterobacteriaceae that have been associated with the spread of antibiotic resistance. Here, we report draft genome assemblies of an Enterobacter hormaechei clinical isolate and a multidrug-resistant clinical isolate of Klebsiella pneumoniae.

scholarly journals Draft Genome Sequences of the Clinical Isolates Kp 23 and KPM 20

2021 ◽  
Vol 10 (12) ◽  
Author(s):  
Alyssa K. W. Maclean ◽  
Nancy D. Hanson
Keyword(s):  
Klebsiella Pneumoniae ◽  
Outer Membrane ◽  
Clinical Isolate ◽  
Draft Genome ◽  
Clinical Isolates ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Klebsiella pneumoniae strains are capable of becoming resistant through multiple mechanisms. Here, we announce draft sequences for Kp 23, a clinical isolate with no plasmid-encoded β-lactamases, and KPM 20, a clinical isolate with no plasmid-encoded β-lactamases and no detectable OmpK35, OmpK36, or PhoE in the outer membrane.

scholarly journals Multidrug-Resistant Enterococci Lack CRISPR-cas

mBio ◽  
2010 ◽  
Vol 1 (4) ◽  
Author(s):  
Kelli L. Palmer ◽  
Michael S. Gilmore
Keyword(s):  
Antibiotic Resistance ◽  
Draft Genome ◽  
Inverse Correlation ◽  
Multidrug Resistant ◽  
Mobile Elements ◽  
Mobile Dna ◽  
Data Set ◽  
Content Type ◽  
Genome Defense ◽  
Acquired Antibiotic Resistance

ABSTRACT Clustered, regularly interspaced short palindromic repeats (CRISPR) provide bacteria and archaea with sequence-specific, acquired defense against plasmids and phage. Because mobile elements constitute up to 25% of the genome of multidrug-resistant (MDR) enterococci, it was of interest to examine the codistribution of CRISPR and acquired antibiotic resistance in enterococcal lineages. A database was built from 16 Enterococcus faecalis draft genome sequences to identify commonalities and polymorphisms in the location and content of CRISPR loci. With this data set, we were able to detect identities between CRISPR spacers and sequences from mobile elements, including pheromone-responsive plasmids and phage, suggesting that CRISPR regulates the flux of these elements through the E. faecalis species. Based on conserved locations of CRISPR and CRISPR-cas loci and the discovery of a new CRISPR locus with associated functional genes, CRISPR3-cas, we screened additional E. faecalis strains for CRISPR content, including isolates predating the use of antibiotics. We found a highly significant inverse correlation between the presence of a CRISPR-cas locus and acquired antibiotic resistance in E. faecalis, and examination of an additional eight E. faecium genomes yielded similar results for that species. A mechanism for CRISPR-cas loss in E. faecalis was identified. The inverse relationship between CRISPR-cas and antibiotic resistance suggests that antibiotic use inadvertently selects for enterococcal strains with compromised genome defense. IMPORTANCE For many bacteria, including the opportunistically pathogenic enterococci, antibiotic resistance is mediated by acquisition of new DNA and is frequently encoded on mobile DNA elements such as plasmids and transposons. Certain enterococcal lineages have recently emerged that are characterized by abundant mobile DNA, including numerous viruses (phage), and plasmids and transposons encoding multiple antibiotic resistances. These lineages cause hospital infection outbreaks around the world. The striking influx of mobile DNA into these lineages is in contrast to what would be expected if a self (genome)-defense system was present. Clustered, regularly interspaced short palindromic repeat (CRISPR) defense is a recently discovered mechanism of prokaryotic self-defense that provides a type of acquired immunity. Here, we find that antibiotic resistance and possession of complete CRISPR loci are inversely related and that members of recently emerged high-risk enterococcal lineages lack complete CRISPR loci. Our results suggest that antibiotic therapy inadvertently selects for enterococci with compromised genome defense.

scholarly journals Comparative Genomics of Klebsiella pneumoniae Strains with Different Antibiotic Resistance Profiles

2011 ◽  
Vol 55 (9) ◽  
pp. 4267-4276 ◽  
Author(s):  
Vinod Kumar ◽  
Peng Sun ◽  
Jessica Vamathevan ◽  
Yong Li ◽  
Karen Ingraham ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Susceptible Strain ◽  
Clinical Isolates ◽  
Whole Genome ◽  
Content Type ◽  
Extended Spectrum

ABSTRACTThere is a global emergence of multidrug-resistant (MDR) strains ofKlebsiella pneumoniae, a Gram-negative enteric bacterium that causes nosocomial and urinary tract infections. While the epidemiology ofK. pneumoniaestrains and occurrences of specific antibiotic resistance genes, such as plasmid-borne extended-spectrum β-lactamases (ESBLs), have been extensively studied, only four complete genomes ofK. pneumoniaeare available. To better understand the multidrug resistance factors inK. pneumoniae, we determined by pyrosequencing the nearly complete genome DNA sequences of two strains with disparate antibiotic resistance profiles, broadly drug-susceptible strain JH1 and strain 1162281, which is resistant to multiple clinically used antibiotics, including extended-spectrum β-lactams, fluoroquinolones, aminoglycosides, trimethoprim, and sulfamethoxazoles. Comparative genomic analysis of JH1, 1162281, and other publishedK. pneumoniaegenomes revealed a core set of 3,631 conserved orthologous proteins, which were used for reconstruction of whole-genome phylogenetic trees. The close evolutionary relationship between JH1 and 1162281 relative to otherK. pneumoniaestrains suggests that a large component of the genetic and phenotypic diversity of clinical isolates is due to horizontal gene transfer. Using curated lists of over 400 antibiotic resistance genes, we identified all of the elements that differentiated the antibiotic profile of MDR strain 1162281 from that of susceptible strain JH1, such as the presence of additional efflux pumps, ESBLs, and multiple mechanisms of fluoroquinolone resistance. Our study adds new and significant DNA sequence data onK. pneumoniaestrains and demonstrates the value of whole-genome sequencing in characterizing multidrug resistance in clinical isolates.

scholarly journals Draft Genome Sequence of Pseudomonas aeruginosa Strain BWH047, a Sequence Type 235 Multidrug-Resistant Clinical Isolate Expressing High Levels of Colistin Resistance

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
Kelly E. R. Bachta ◽  
Egon A. Ozer ◽  
Alisha Pandit ◽  
Francisco M. Marty ◽  
John J. Mekalanos ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Genome Sequence ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Colistin Resistance ◽  
Content Type ◽  
Global Epidemic

The Gram-negative bacterium Pseudomonas aeruginosa is often multidrug resistant, associated with global epidemic outbreaks, and responsible for significant morbidity and mortality in hospitalized patients. Here, we present the draft genome sequence of BWH047, a multidrug-resistant P. aeruginosa clinical isolate belonging to the epidemic sequence type 235 and demonstrating high levels of colistin resistance.

scholarly journals Draft Genome Sequences of Clinical Isolates of Multidrug-Resistant Acinetobacter baumannii

2017 ◽  
Vol 5 (5) ◽  
Author(s):  
Keesha E. Erickson ◽  
Nancy E. Madinger ◽  
Anushree Chatterjee
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Genome Sequences ◽  
Content Type ◽  
University Of Colorado ◽  
The University

ABSTRACT We report here the draft genome sequences of two clinically isolated Acinetobacter baumannii strains. These samples were obtained from patients at the University of Colorado Hospital in 2007 and 2013 and encode an estimated 20 and 13 resistance genes, respectively.

scholarly journals Draft Genome Sequences of Two Multidrug-Resistant Sequence Type 405 Escherichia coli Isolates without Clinical Infection

2021 ◽  
Vol 10 (31) ◽  
Author(s):  
Amanda Chamieh ◽  
Rita Zgheib ◽  
Sabah El-Sawalhi ◽  
Eid Azar ◽  
Jean-Marc Rolain
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Clinical Infection ◽  
Genome Sequences ◽  
Content Type

We present the genome sequences of two carbapenemase-producing sequence type 405 Escherichia coli clinical isolates, strains Marseille-Q1950 and Marseille-Q1951. The isolates were obtained 1 month apart during the patient’s hospitalization in Lebanon, in May (Marseille-Q1950) and June (Marseille-Q1951) 2019. The genome sizes of strains Marseille-Q1950 and Marseille-Q1951 were 5,181,515 bp and 5,213,451 bp, respectively.

scholarly journals Draft Genomic Sequence of Multidrug-Resistant Klebsiella pneumoniae B8S35, Isolated from Retail Chicken Skin

2019 ◽  
Vol 8 (28) ◽  
Author(s):  
Aixia Xu ◽  
William Mackay ◽  
Christopher H. Sommers
Keyword(s):  
Health Care ◽  
Klebsiella Pneumoniae ◽  
Quaternary Ammonium ◽  
Genomic Sequence ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Content Type ◽  
Chicken Skin ◽  
Ammonium Compounds ◽  
Multiple Antibiotics

Here, we report the draft genome sequence of Klebsiella pneumoniae strain B8S35, isolated from retail chicken skin. It carries genes for resistance to multiple antibiotics, as well as quaternary ammonium compounds used by the food and health care industries.

scholarly journals Draft Genome Sequences of Klebsiella pneumoniae Clinical Type Strain ATCC 13883 and Three Multidrug-Resistant Clinical Isolates

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Brock A. Arivett ◽  
David C. Ream ◽  
Steven E. Fiester ◽  
Katrin Mende ◽  
Clinton K. Murray ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Type Strain ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Strain Atcc ◽  
Genome Sequences ◽  
Clinical Type

scholarly journals Draft Genome Sequences of Nonclinical and Clinical Enterobacter cloacae Isolates Exhibiting Multiple Antibiotic Resistance and Virulence Factors

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Mitali Mishra ◽  
Shashank Patole ◽  
Harapriya Mohapatra
Keyword(s):  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Draft Genome ◽  
Enterobacter Cloacae ◽  
Multidrug Resistant ◽  
Multiple Antibiotic Resistance ◽  
Opportunistic Pathogens ◽  
Genome Sequences ◽  
Content Type ◽  
Therapeutic Drugs

ABSTRACT Enterobacter spp. have been implicated as opportunistic pathogens which over the years have gained resistance toward most of the available therapeutic drugs. We sequenced two multidrug-resistant Enterobacter cloacae isolates harboring multiple efflux pump genes. These isolates exhibited strain-specific modulation of efflux pump protein expression.

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