scholarly journals Early Relaxation Dynamics in the LC 13 T Cell Receptor in Reaction to 172 Altered Peptide Ligands: A Molecular Dynamics Simulation Study

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e64464 ◽  
Author(s):  
Bernhard Knapp ◽  
Georg Dorffner ◽  
Wolfgang Schreiner
Keyword(s):  
Molecular Dynamics ◽  
T Cell ◽  
Molecular Dynamics Simulation ◽  
T Cell Receptor ◽  
Simulation Study ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Dynamics Simulation ◽  
Relaxation Dynamics ◽  
Altered Peptide Ligands

scholarly journals Intramolecular Domain Movements of Free and Bound pMHC and TCR Proteins: A Molecular Dynamics Simulation Study

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 720 ◽  
Author(s):  
Rudolf Karch ◽  
Claudia Stocsits ◽  
Nevena Ilieva ◽  
Wolfgang Schreiner
Keyword(s):  
Molecular Dynamics ◽  
T Cell ◽  
Molecular Dynamics Simulation ◽  
Simulation Study ◽  
Bound States ◽  
Cell Receptor ◽  
Dynamics Simulation ◽  
Antigenic Peptides ◽  
Major Histocompatibility Complexes ◽  
Unbound States

The interaction of antigenic peptides (p) and major histocompatibility complexes (pMHC) with T-cell receptors (TCR) is one of the most important steps during the immune response. Here we present a molecular dynamics simulation study of bound and unbound TCR and pMHC proteins of the LC13-HLA-B*44:05-pEEYLQAFTY complex to monitor differences in relative orientations and movements of domains between bound and unbound states of TCR-pMHC. We generated local coordinate systems for MHC α1- and MHC α2-helices and the variable T-cell receptor regions TCR Vα and TCR Vβ and monitored changes in the distances and mutual orientations of these domains. In comparison to unbound states, we found decreased inter-domain movements in the simulations of bound states. Moreover, increased conformational flexibility was observed for the MHC α2-helix, the peptide, and for the complementary determining regions of the TCR in TCR-unbound states as compared to TCR-bound states.

scholarly journals Structural basis for T cell recognition of altered peptide ligands: a single T cell receptor can productively recognize a large continuum of related ligands.

1996 ◽  
Vol 184 (4) ◽  
pp. 1259-1268 ◽  
Author(s):  
G J Kersh ◽  
P M Allen
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Clone ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Structural Basis ◽  
Side Chain ◽  
Contact Residue ◽  
Altered Peptide Ligands ◽  
T Cell Clone

T cells recognize short linear peptides bound to major histocompatibility complex (MHC)-encoded molecules. Subtle molecular changes in peptide antigens produce altered peptide ligands (APLs), which induce different T cell responses from those induced by the antigenic ligand. A molecular basis for how these slight molecular variations lead to such different consequences for the T cell has not been described. To address this issue, we have made amino acid substitutions at the primary T cell receptor (TCR) contact residue of the murine hemoglobin determinant, Hb(64-76)/I-Ek and produced 12 peptides that interact with the TCR of the T cell clone 3.L2. The 3.L2 T cell responds to these peptides, which vary 1 million-fold in their activity, and enables them to be ranked according to their relative ability to signal through the 3.L2 TCR. Such a ranking reveals that the ability of the 3.L2 T cell to respond to these peptides depends on how well the structure of the side chain at the primary TCR contact site mimics that of the Asn residue present in the antigenic ligand. The reactivity of the 3.L2 T cell also depends on an MHC contact residue that is next to the primary TCR contact residue, suggesting that conformation of the Asn side chain is also important. By using nonnatural amino acids at a TCR contact residue, we have demonstrated that APLs can be rationally designed based on structure. These data are consistent with a model in which the affinity of a peptide-MHC complex for the TCR determines how the T cell will respond.

scholarly journals Altered Peptide Ligands Induce Delayed CD8-T Cell Receptor Interaction—a Role for CD8 in Distinguishing Antigen Quality

Immunity ◽  
2006 ◽  
Vol 25 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Pia P. Yachi ◽  
Jeanette Ampudia ◽  
Tomasz Zal ◽  
Nicholas R.J. Gascoigne
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cd8 T Cell ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Receptor Interaction ◽  
Altered Peptide Ligands

scholarly journals High-affinity T cell receptor differentiates cognate peptide–MHC and altered peptide ligands with distinct kinetics and thermodynamics

2010 ◽  
Vol 47 (9) ◽  
pp. 1793-1801 ◽  
Author(s):  
Stephen P. Persaud ◽  
David L. Donermeyer ◽  
K. Scott Weber ◽  
David M. Kranz ◽  
Paul M. Allen
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Altered Peptide Ligands ◽  
Kinetics And Thermodynamics ◽  
High Affinity

scholarly journals Altered Peptide Ligands Impact the Diversity of Polyfunctional Phenotypes in T Cell Receptor Gene-Modified T Cells

2018 ◽  
Vol 26 (4) ◽  
pp. 996-1007 ◽  
Author(s):  
Timothy T. Spear ◽  
Yuan Wang ◽  
Thomas W. Smith ◽  
Patricia E. Simms ◽  
Elizabeth Garrett-Mayer ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Receptor Gene ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Altered Peptide Ligands ◽  
T Cell Receptor Gene ◽  
Cell Receptor Gene

scholarly journals Cd4+ T Cells from Lupus-Prone Mice Are Hyperresponsive to T Cell Receptor Engagement with Low and High Affinity Peptide Antigens

2001 ◽  
Vol 193 (3) ◽  
pp. 329-338 ◽  
Author(s):  
George S. Vratsanos ◽  
Sungsoo Jung ◽  
Yeong-Min Park ◽  
Joe Craft
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
T Cell Activation ◽  
Cell Activation ◽  
Interleukin 2 ◽  
Cell Receptor ◽  
Peptide Ligands ◽  
Altered Peptide Ligands ◽  
Lower Affinity

Polyclonal CD4+ T cell activation is characteristic of spontaneous lupus. As a potential explanation for this phenotype, we hypothesized that T cells from lupus-prone mice are intrinsically hyperresponsive to stimulation with antigen, particularly to those peptide ligands having a low affinity for the T cell receptor (TCR). To test this hypothesis, we backcrossed the α and β chain genes of the AND TCR specific for amino acids 88–104 of pigeon cytochrome C (PCC) to the Fas-intact MRL/Mp+Fas-lpr and to the H-2k–matched control backgrounds B10.BR and CBA/CaJ (MRL.AND, B10.AND, and CBA.AND, respectively), and assessed naive CD4+ TCR transgenic T cell activation in vitro after its encounter with cognate antigen and lower affinity altered peptide ligands (APLs). MRL.AND T cells, compared with control B10.AND and CBA.AND cells, proliferated more when stimulated with agonist antigen. More strikingly, MRL.AND T cells proliferated significantly more and produced more interleukin 2 when stimulated with the APLs of PCC 88–104, having lower affinity for the transgenic TCR. These results imply that one of the forces driving polyclonal activation of α/β T cells in lupus is an intrinsically heightened response to peptide antigen, particularly those with low affinity for the TCR, independent of the nature of the antigen-presenting cell and degree of costimulation.

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