Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

Abstract MicroRNAs (miRNA) have been shown to regulate plant pathogen interaction, by silencing genes, destructing or blocking of the translation of mRNA. However, their role in bacterial wilt, caused by Ralstonia solanacearum in ginger, has not been studied. In the present study, we utilized the transcriptome data from ginger-Ralstonia solanacearum interactions to characterize miRNAs from bacterial wilt-susceptible ginger (Zingiber officinale) and resistant mango ginger (Curcuma amada). The assembled mRNAs were utilized to generate miRNA targets and miRNAs. Considering the alignment results, we located a total of 2926 potential miRNA targets out of which 1551 were upregulated and 1419 were downregulated in ginger. In case of mango ginger, out of 2145 potential miRNA targets, 1506 were upregulated and 1594 were downregulated. In the resistance interactions with mango ginger, 1068 unique target genes were upregulated when compared to control. Gene Ontology (GO) analysis of differentially expressed target genes showed highest enrichment in response to cold, chloroplast and ATP binding in biological, cellular and molecular functions respectively. Nine target genes and their corresponding miRNAs were experimentally validated, which shows significant difference in expression with ginger-Ralstonia solanacearum interactions. The results will be very useful to disease resistant varieties of ginger.

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Transcriptome-wide identification and characterization of resistant gene analogs (RGAs) of ginger (Zingiber officinale Rosc.) and mango ginger (Curcuma amada Roxb.) under stress induced by pathogen

Scientia Horticulturae ◽

10.1016/j.scienta.2019.01.003 ◽

2019 ◽

Vol 248 ◽

pp. 81-88 ◽

Cited By ~ 1

Author(s):

Karthika R ◽

Prasath D ◽

Anandaraj M

Keyword(s):

Zingiber Officinale ◽

Mango Ginger ◽

Curcuma Amada ◽

Resistant Gene ◽

Identification And Characterization

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Transcriptomic analysis to reveal the differentially expressed miRNA targets and their miRNAs in response to Ralstonia solanacearum in ginger species

BMC Plant Biology ◽

10.1186/s12870-021-03108-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Mohandas Snigdha ◽

Duraisamy Prasath

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Target Genes ◽

Mrna Translation ◽

Zingiber Officinale ◽

Vital Role ◽

Integrated Analysis ◽

Data Set ◽

Mango Ginger ◽

Plant Pathogen Interaction

Abstract Background Bacterial wilt is the most devastating disease in ginger caused by Ralstonia solanacearum. Even though ginger (Zingiber officinale) and mango ginger (Curcuma amada) are from the same family Zingiberaceae, the latter is resistant to R. solanacearum infection. MicroRNAs have been identified in many crops which regulates plant-pathogen interaction, either through silencing genes or by blocking mRNA translation. However, miRNA’s vital role and its targets in mango ginger in protecting bacterial wilt is not yet studied extensively. In the present study, using the “psRNATarget” server, we analyzed available ginger (susceptible) and mango ginger (resistant) transcriptome to delineate and compare the microRNAs (miRNA) and their target genes (miRTGs). Results A total of 4736 and 4485 differential expressed miRTGs (DEmiRTGs) were identified in ginger and mango ginger, respectively, in response to R. solanacearum. Functional annotation results showed that mango ginger had higher enrichment than ginger in top enriched GO terms. Among the DEmiRTGs, 2105 were common in ginger and mango ginger. However, 2337 miRTGs were expressed only in mango ginger which includes 62 defence related and upregulated miRTGs. We also identified 213 miRTGs upregulated in mango ginger but downregulated in ginger, out of which 23 DEmiRTGS were defence response related. We selected nine miRNA/miRTGs pairs from the data set of common miRTGs of ginger and mango ginger and validated using qPCR. Conclusions Our data covered the expression information of 9221 miRTGs. We identified nine miRNA/miRTGs key candidate pairs in response to R. solanacearum infection in ginger. This is the first report of the integrated analysis of miRTGs and miRNAs in response to R. solanacearum infection among ginger species. This study is expected to deliver several insights in understanding the miRNA regulatory network in ginger and mango ginger response to bacterial wilt.

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Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers

Electronic Journal of Biotechnology ◽

10.2225/vol13-issue6-fulltext-10 ◽

2010 ◽

Vol 13 (6) ◽

pp. 0-0 ◽

Cited By ~ 2

Author(s):

Shakeel Ahmad Jatoi ◽

Akira Kikuchi ◽

Dawood Ahmad ◽

Kazuo N. Watanbe

Keyword(s):

Genetic Structure ◽

Functional Genomic ◽

Mango Ginger ◽

Curcuma Amada ◽

Genomic Markers ◽

Genebank Collection

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Characterization of Volatile Organic Compounds in Mango Ginger (Curcuma amada Roxb.) from Myanmar

Metabolites ◽

10.3390/metabo11010021 ◽

2020 ◽

Vol 11 (1) ◽

pp. 21

Author(s):

Yanhang Chen ◽

Musavvara Kh. Shukurova ◽

Yonathan Asikin ◽

Miyako Kusano ◽

Kazuo N. Watanabe

Keyword(s):

Solid Phase ◽

Species Variation ◽

Mango Ginger ◽

Food Industries ◽

Curcuma Amada ◽

Alternative Medicines ◽

Flight Mass Spectrometry ◽

Volatile Organic ◽

Loss Of Appetite

Curcuma amada Roxb. (Zingiberaceae), commonly known as mango ginger because its rhizome and foliar parts have a similar aroma to mango. The rhizome has been widely used in food industries and alternative medicines to treat a variety of internal diseases such as cough, bronchitis, indigestion, colic, loss of appetite, hiccups, and constipation. The composition of the volatile constituents in a fresh rhizome of C. amada is not reported in detail. The present study aimed to screen and characterize the composition of volatile organic compound (VOC) in a fresh rhizome of three C. amada (ZO45, ZO89, and ZO114) and one C. longa (ZO138) accessions originated from Myanmar. The analysis was carried out by means of headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS). As a result, 122 VOCs were tentatively identified from the extracted 373 mass spectra. The following compounds were the ten most highly abundant and broadly present ones: ar-turmerone, α-zingiberene, α-santalene, (E)-γ-atlantone, cuparene, β-bisabolene, teresantalol, β-sesquiphellandrene, trans-α-bergamotene, γ-curcumene. The intensity of ar-turmerone, the sesquiterpene which is mainly characterized in C. longa essential oil (up to 15.5–27.5%), was significantly higher in C. amada accession ZO89 (15.707 ± 5.78a) compared to C. longa accession ZO138 (0.300 ± 0.08b). Cis-α-bergamotene was not detected in two C. amada accessions ZO45 and ZO89. The study revealed between-species variation regarding identified VOCs in the fresh rhizome of C. amada and C. longa.

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Effect of Organic Manures on Growth, Yield and Quality of Mango Ginger (Curcuma amada Roxb.) in the Gangetic Alluvial Plains of West Bengal

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2019.811.121 ◽

2019 ◽

Vol 8 (11) ◽

pp. 1030-1034

Author(s):

Anupam Pariari ◽

C.S. Karthik ◽

Sarthak Bhattacharya

Keyword(s):

West Bengal ◽

Growth Yield ◽

Organic Manures ◽

Mango Ginger ◽

Curcuma Amada ◽

Yield And Quality

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Burkholderia solanacearum. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056401220 ◽

1994 ◽

Author(s):

G. S. Saddler

Keyword(s):

Geographical Distribution ◽

Bacterial Wilt ◽

Vascular System ◽

Solanum Melongena ◽

Zingiber Officinale ◽

Modes Of Transmission ◽

Planting Material ◽

Complete Listing ◽

Latently Infected ◽

Plant Families

Abstract A description is provided for Burkholderia solanacearum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: The host range is one of the widest of all the phytopathogenic bacteria. The most susceptible plant family, in terms of numbers of species affected is the Solanaceae; over fifty other plant families contain susceptible species. The most economically significant hosts are listed here. For a more complete listing see Kelman (1953), Bradbury (1986) and Hayward & Hartman (1994). Arachis hypogaea, Capsicum spp., Gossypium hirsutum, Ipomoea batatus, Lycopersicon esculentum, Manihot esculenta, Musa spp., Nicotiana spp., Solanum melongena, Solanum tuberosum & Zingiber officinale. DISEASE: Bacterial wilt. Infection is systemic, producing a wilt of parts or the whole plant. Vascular system may become discoloured, bacterial ooze can be produced and plants may be stunted and chlorotic. GEOGRAPHICAL DISTRIBUTION: Widespread in tropical, subtropical and warm temperate regions of the world (see data sheets on quarantine pests). TRANSMISSION: A variety of modes of transmission exist which are host dependant. Infected planting material and true seed is responsible for the spread of bacterial wilt of banana, ginger, groundnut, potato and tomato, whilst transport of latently infected in seedlings (stawberry) and the actions of insect (Moko disease of banana) and weather (tobacco) have all been implicated. For a review see Kelman et al. (1994).

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