scholarly journals Unravelling the Differential Expression of Potential microRNAs in Bacterial Wilt-resistant and Susceptible Ginger Species

2020 ◽  
Author(s):  
M Snigdha ◽  
D Prasath
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Target Genes ◽  
Zingiber Officinale ◽  
Transcriptome Data ◽  
Mango Ginger ◽  
Mirna Targets ◽  
Resistant Varieties ◽  
Significant Difference ◽  
Plant Pathogen Interaction

Abstract MicroRNAs (miRNA) have been shown to regulate plant pathogen interaction, by silencing genes, destructing or blocking of the translation of mRNA. However, their role in bacterial wilt, caused by Ralstonia solanacearum in ginger, has not been studied. In the present study, we utilized the transcriptome data from ginger-Ralstonia solanacearum interactions to characterize miRNAs from bacterial wilt-susceptible ginger (Zingiber officinale) and resistant mango ginger (Curcuma amada). The assembled mRNAs were utilized to generate miRNA targets and miRNAs. Considering the alignment results, we located a total of 2926 potential miRNA targets out of which 1551 were upregulated and 1419 were downregulated in ginger. In case of mango ginger, out of 2145 potential miRNA targets, 1506 were upregulated and 1594 were downregulated. In the resistance interactions with mango ginger, 1068 unique target genes were upregulated when compared to control. Gene Ontology (GO) analysis of differentially expressed target genes showed highest enrichment in response to cold, chloroplast and ATP binding in biological, cellular and molecular functions respectively. Nine target genes and their corresponding miRNAs were experimentally validated, which shows significant difference in expression with ginger-Ralstonia solanacearum interactions. The results will be very useful to disease resistant varieties of ginger.

scholarly journals Transcriptomic analysis to reveal the differentially expressed miRNA targets and their miRNAs in response to Ralstonia solanacearum in ginger species

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohandas Snigdha ◽  
Duraisamy Prasath
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Target Genes ◽  
Mrna Translation ◽  
Zingiber Officinale ◽  
Vital Role ◽  
Integrated Analysis ◽  
Data Set ◽  
Mango Ginger ◽  
Plant Pathogen Interaction

Abstract Background Bacterial wilt is the most devastating disease in ginger caused by Ralstonia solanacearum. Even though ginger (Zingiber officinale) and mango ginger (Curcuma amada) are from the same family Zingiberaceae, the latter is resistant to R. solanacearum infection. MicroRNAs have been identified in many crops which regulates plant-pathogen interaction, either through silencing genes or by blocking mRNA translation. However, miRNA’s vital role and its targets in mango ginger in protecting bacterial wilt is not yet studied extensively. In the present study, using the “psRNATarget” server, we analyzed available ginger (susceptible) and mango ginger (resistant) transcriptome to delineate and compare the microRNAs (miRNA) and their target genes (miRTGs). Results A total of 4736 and 4485 differential expressed miRTGs (DEmiRTGs) were identified in ginger and mango ginger, respectively, in response to R. solanacearum. Functional annotation results showed that mango ginger had higher enrichment than ginger in top enriched GO terms. Among the DEmiRTGs, 2105 were common in ginger and mango ginger. However, 2337 miRTGs were expressed only in mango ginger which includes 62 defence related and upregulated miRTGs. We also identified 213 miRTGs upregulated in mango ginger but downregulated in ginger, out of which 23 DEmiRTGS were defence response related. We selected nine miRNA/miRTGs pairs from the data set of common miRTGs of ginger and mango ginger and validated using qPCR. Conclusions Our data covered the expression information of 9221 miRTGs. We identified nine miRNA/miRTGs key candidate pairs in response to R. solanacearum infection in ginger. This is the first report of the integrated analysis of miRTGs and miRNAs in response to R. solanacearum infection among ginger species. This study is expected to deliver several insights in understanding the miRNA regulatory network in ginger and mango ginger response to bacterial wilt.

scholarly journals Selection and Inheritance of Tomato Resistance against Ralstonia solanacearum

2019 ◽  
Vol 23 (1) ◽  
pp. 61
Author(s):  
Isna Maulida ◽  
Rudi Hari Murti ◽  
Triwidodo Arwiyanto
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Dominant Gene ◽  
Tomato Plants ◽  
Breeding Lines ◽  
Back Cross ◽  
Inheritance Patterns ◽  
Randomized Design ◽  
Resistant Varieties ◽  
Resistant Lines

Ralstonia solanacearum is a plant pathogen causes wilting which is a major obstacle in the cultivation of tomato plants. In plant breeding, knowledge of the source of resistance genes and inheritance patterns is important in the development of bacterial wilt resistant varieties. This study aimed to obtain bacterial wilt resistant lines and to find out the inheritance pattern of tomato resistance to bacterial wilt. Selection of resistant plant involved the selected breeding lines from irradiation and crossing collections of the Genetic Laboratory, Faculty of Agriculture, Universitas Gadjah Mada. Introduced lines of H-7996 and F1 Permata and Timoti were used as a control. H-7996 as resistant parents and GM2 as susceptible parents, and their offspring include F1 GM2 x H-7996, F1 reciprocal, F2, Back Cross 1 (F1 x GM2), and Back Cross 2 (F1 x H-7996) used in testing inheritance patterns. Inoculation was carried out 1 week after planting by pouring 100 ml of water suspension of R. solanacarum (108  cfu/ml) on the roots. Completely Randomized Design (CRD) was used in this experiment. The scoring observation was carried out every week for one month. This study showed that Permata as a control was the most resistant, while Timoti and H-7996 were medium resistant. The CLN, G6, G8, and G7 lines were susceptible medium, yet only G8 and G7 with the smallest percentage of disease intensity and not significantly different than Timoti. The resistance gene to bacterial wilt on H-7996 was controlled by genes in the cell nucleus with additive-dominant gene action. Resistance to bacteria has a moderate level of heritability.

scholarly journals Evaluation of the antibacterial activity of mango ginger rhizome extracts against bacterial wilt pathogen Ralstonia solanacearum

1970 ◽  
pp. 101
Author(s):  
R Karthika, D Prasath*, N K Leela, R Suseela Bhai, M Anandaraj
Keyword(s):  
Essential Oils ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Resistance Mechanism ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Mango Ginger ◽  
Methanol Extracts ◽  
Bacterial Wilt Disease ◽  
Ginger Rhizome

The Indian mango ginger (Curcuma amada Roxb.) is a perennial rhizomatous herb with a raw mango flavour. It is resistant to bacterial wilt disease. In order to understand the disease resistance mechanism of mango gingerthe hexane, chloroform and methanol extracts (5, 10, 25, 50 and 100mg/ml) and essential oils (1%, 5% and 10%) were tested against the bacterial wilt pathogen Ralstonia solanacearum by agar well diffusion method. The hexane, chloroform and methanol extracts showed more or less the same level of antimicrobial activity with a zone of inhibition ranging from 3-9 mm. The essential oils exhibited a zone of inhibition ranging from 3-7 mm.The major constituents of the essential oils were β-myrcene and β-pinene. The results indicated that the rhizome of mango ginger may contain compounds that are toxic to the pathogen. The extracts of mango ginger could be explored further for developing a natural bactericide against R. solanacearum.

scholarly journals Pengaruh Tomat Sambung Pada Intensitas Penyakit Layu Bakteri (Ralstonia solanacearum), Komponen Hasil Produksi, dan Kualitas Buah

2021 ◽  
Vol 26 (3) ◽  
pp. 413-420
Author(s):  
Lisa Navitasari ◽  
Tri Joko ◽  
Rudi Hari Murti ◽  
Triwidodo Arwiyanto
Keyword(s):  
Fruit Quality ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Bacterial Wilt Disease ◽  
Resistant Varieties ◽  
Soluble Solid ◽  
Increase Productivity ◽  
High Production ◽  
Resistant Rootstock

Ralstonia solanacearum (Smith) is one of soil borne pathogens causes bacterial wilt diseases and R. solanacearum is difficult to control because it has a long survival in the soil and have many hosts alternatives. One alternative to control R. solanacearum and to increase productivity is by using grating with combination of resistant varieties of tomato as a rootstock and high production varieties of tomato as a scion. Several studies on grafting to suppress R. solanacearum were reported. However, study on grafting with combination between resistant tomato varieties (Amelia H7996) and high-production tomato varieties with R. solanacearum infestation and without R. solanacearum infestation on the component of yield and fruit quality is limited. The study aims to analyze the effect of grafting with R. solanacearum infestation to the intensity of bacterial wilt disease, component of yield, and fruit quality with R. solanacearum infestation and without R. solanacearum infestation. The result indicated that the intensity of bacterial wilt disease on grafted tomato did not significantly different from resistant rootstock but significantly different from scion.  Infestation of R. solanacearum on grafted tomato can decreased the plant productivity that decreased the component of yield on grafted tomato. R. solanacearum infestation also decreased the fruit quality on diameters and fruit firmness. Nevertheless, R. solanacearum did not affect the Total soluble solid/TSS (°Brix). TSS on grafted tomato indicated that the value is taller than rootstock in the field with R. solanacearum infestation and in the field without R. solanacearum infestation.   Keywords: fruit quality, productivity, Rasltonia solanacearum, tomato grafted

scholarly journals PENGARUH PERLAKUAN BIBIT TERHADAP PERKEMBANGAN PENYAKIT LAYU BAKTERI PADA JAHE (ZINGIBER OFFICINALE)

2002 ◽  
Vol 2 (2) ◽  
pp. 60-64
Author(s):  
Titik Nur Aeny ◽  
Feriansyah Feriansyah ◽  
Subli Mujim
Keyword(s):  
Incubation Period ◽  
Analysis Of Variance ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Zingiber Officinale ◽  
Wilt Disease ◽  
Factorial Experiment ◽  
The Other ◽  
Bacterial Wilt Disease ◽  
Two Factors

Effect of seedling treatments on development of bacterial wilt disease of ginger (Zingiber officinale).   This experiment was aimed to study the effect of  seedling treatment with bactericide on incubation period and disease intensity of bacterial wilt caused by  Ralstonia solanacearum on ginger (Zingiber officinale).  The experiment was conducted from May to November 2001, and the treatments were set as factorial experiment in completely randomised design.  The first factor was the level of bactericide concentrations and the second factor was the length of soaking periods.  Each treatment consisted of  five plants and  was replicated three times.  Data were analysed with ANOVA and the means were compared with Duncan test.  The results of analysis of variance showed that the interaction of  bactericide concentrations with soaking periods was not significantly affected the incubation period and the intensity of bacterial wilt disease of ginger. However,  those two factors significantly (P < 0.01) affected  the incubation period and the intensity of ginger bacterial wilt disease.   Separation of the means by Duncan test indicated that the higher the bactericide concentration, the longer the incubation period. On the other hand, the disease intensity was lowered by the higher concentration.  The effect of  bactericide concentration 4 g/l was not significantly different from that of 6 g/l.   The analysis of variance was also showed that the length of soaking period of ginger seedling in bactericide did not affect the incubation period  nor the disease intensity.

scholarly journals Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Lisa Navitasari ◽  
TRI JOKO ◽  
RUDI HARI MURTI ◽  
TRIWIDODO ARWIYANTO
Keyword(s):  
Community Structure ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Distinct Region ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Significant Difference

Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.

scholarly journals Comparison of the Transcriptomes of Ginger (Zingiber officinale Rosc.) and Mango Ginger (Curcuma amada Roxb.) in Response to the Bacterial Wilt Infection

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99731 ◽  
Author(s):  
Duraisamy Prasath ◽  
Raveendran Karthika ◽  
Naduva Thadath Habeeba ◽  
Erinjery Jose Suraby ◽  
Ottakandathil Babu Rosana ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Zingiber Officinale ◽  
Mango Ginger ◽  
Curcuma Amada

scholarly journals CHARACTERIZATION OF EGGPLANT ENDOPHYTE BACTERIA AND RHIZOBACTERIA AS WELL AS THEIR ANTAGONISTIC ABILITY AGAINST Ralstonia solanacearum

2020 ◽  
Vol 20 (2) ◽  
pp. 150-156
Author(s):  
Larasati Puspita Saridewi ◽  
Nur Prihatiningsih ◽  
Heru Adi Djatmiko
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Morphological Characteristics ◽  
In Vitro Test ◽  
Randomized Design ◽  
Resistant Varieties ◽  
Glucose Agar ◽  
Vitro Test

Characterization of eggplant endophyte bacteria and rhizobacteria as well as their antagonistic ability against Ralstonia solanacearum. Bacterial wilt caused by Ralsonia solanacearum is one of important diseases causing severe loses in eggplant production. Various strategies were used to manage bacterial wilt, including planting resistant varieties, soil amandement, and soil solarization. However, management of R. solanacearum in eggplant by using endophytic bacteria and rhizobacteria were not been done that much. The objective of this study was to: (1) characterization of endophytic and rhizobacteria; (2) determines the inhibition ability of endophytic and rhizobacteria isolates against R. solanacearum pathogen on eggplant. The laboratory experiment was arranged in completely randomized design with 5 treatments and 5 replications. The double layer method using yeast peptone glucose agar (YPGA) medium was used in vitro test. Based on the morphological characteristics these isolates were suspected as a member of genus Bacillus. Among the isolates used in this study, TK isolate showed the best capability to inhibit growth of R. solanacearum.

Sign in / Sign up

Export Citation Format

Share Document