Nuclear-Cytoplasmic Conflict in Pea (Pisum sativum L.) Is Associated with Nuclear and Plastidic Candidate Genes Encoding Acetyl-CoA Carboxylase Subunits

A genomic clone from pea (Pisum sativum L.) contains all of one gene encoding a ‘minor’ (B-type) legumin polypeptide, and most of a second very similar gene. The two genes, designated LegJ and LegK, are arranged in tandem, separated by approx. 6 kb. A complete sequence of gene LegJ and its flanking sequences is given, with as much of the sequence of gene LegK as is present on the genomic clone. Hybridization of 3′ flanking sequence probes to seed mRNA, and sequence comparisons with cDNA species, suggested that gene LegJ, and probably gene LegK, was expressed. The partial amino acid sequences of ‘minor’ legumin α- and beta-polypeptides were used to confirm the identity of these genes. The transciption start in gene LegJ was mapped. The 5′ flanking sequence of gene LegJ contains a sequence conserved in legumin genes from pea and other species, which is likely to have functional significance in control of gene expression. Sequence comparisons with legumin genes and cDNA species from Vicia faba and soya bean show that separation of legumin genes into A- and B-type subfamilies occurred before separation of the Viciae and Glycinae tribes.

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The origin of Triticum petropavlovskyi Udacz. et Migusch.: demonstration of the utility of the genes encoding plastid acetyl-CoA carboxylase sequence

Molecular Breeding ◽

10.1007/s11032-009-9337-0 ◽

2009 ◽

Vol 25 (3) ◽

pp. 381-395 ◽

Cited By ~ 3

Author(s):

Hou Yang Kang ◽

Xing Fan ◽

Hai Qin Zhang ◽

Li Na Sha ◽

Gen Lou Sun ◽

...

Keyword(s):

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Triticum Petropavlovskyi ◽

Genes Encoding

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Molecular Cloning and Characterization of the pyrB1 and pyrB2 Genes Encoding Aspartate Transcarbamoylase in Pea (Pisum sativum L.)

PLANT PHYSIOLOGY ◽

10.1104/pp.105.1.377 ◽

1994 ◽

Vol 105 (1) ◽

pp. 377-384 ◽

Cited By ~ 18

Author(s):

C. L. Williamson ◽

R. D. Slocum

Keyword(s):

Pisum Sativum ◽

Molecular Cloning ◽

Aspartate Transcarbamoylase ◽

Pisum Sativum L ◽

Genes Encoding

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Genome-wide association studies of mineral and phytic acid concentrations in pea (Pisum sativum L.) to evaluate biofortification potential

G3 Genes|Genome|Genetics ◽

10.1093/g3journal/jkab227 ◽

2021 ◽

Author(s):

Sarah Powers ◽

J Lucas Boatwright ◽

Dil Thavarajah

Keyword(s):

Pisum Sativum ◽

Candidate Genes ◽

Phytic Acid ◽

Reference Genome ◽

Genome Wide Association ◽

Genome Wide Association Studies ◽

Single Plant ◽

Pisum Sativum L ◽

Genome Wide ◽

A Genome

Abstract Pea (Pisum sativum L.) is an important cool season food legume for sustainable food production and human nutrition due to its nitrogen fixation capabilities and nutrient-dense seed. However, minimal breeding research has been conducted to improve the nutritional quality of the seed for biofortification, and most genomic-assisted breeding studies utilize small populations with few single nucleotide polymorphisms (SNPs). Genomic resources for pea have lagged behind those of other grain crops, but the recent release of the Pea Single Plant Plus Collection (PSPPC) and the pea reference genome provide new tools to study nutritional traits for biofortification. Calcium, phosphorus, potassium, iron, zinc, and phytic acid concentrations were measured in a study population of 299 different accessions grown under greenhouse conditions. Broad phenotypic variation was detected for all parameters except phytic acid. Calcium exhibited moderate broad-sense heritability (H2) estimates, at 50%, while all other minerals exhibited low heritability. Of the accessions used, 267 were previously genotyped in the PSPPC release by the USDA, and we mapped the genotyping data to the pea reference genome for the first time. This study generated 54,344 high-quality SNPs used to investigate the population structure of the Pea Single Plant Plus Collection and perform a genome-wide association study to identify genomic loci associated with mineral concentrations in mature pea seed. Overall, we were able to identify multiple significant SNPs and candidate genes for iron, phosphorus, and zinc. These results can be used for genetic improvement in pea for nutritional traits and biofortification, and the candidate genes provide insight into mineral metabolism.

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DNA Methylation Patterns Differ between Free-Living Rhizobium leguminosarum RCAM1026 and Bacteroids Formed in Symbiosis with Pea (Pisum sativum L.)

Microorganisms ◽

10.3390/microorganisms9122458 ◽

2021 ◽

Vol 9 (12) ◽

pp. 2458

Author(s):

Alexey M. Afonin ◽

Emma S. Gribchenko ◽

Evgeny A. Zorin ◽

Anton S. Sulima ◽

Vladimir A. Zhukov

Keyword(s):

Pisum Sativum ◽

Rhizobium Leguminosarum ◽

Specific Pattern ◽

Nitrogen Fixing ◽

Free Living ◽

Pisum Sativum L ◽

Genome Methylation ◽

Oxford Nanopore ◽

Genes Encoding ◽

Methylation Patterns

Rhizobium leguminosarum (Rl) is a common name for several genospecies of rhizobia able to form nitrogen-fixing nodules on the roots of pea (Pisum sativum L.) while undergoing terminal differentiation into a symbiotic form called bacteroids. In this work, we used Oxford Nanopore sequencing to analyze the genome methylation states of the free-living and differentiated forms of the Rl strain RCAM1026. The complete genome was assembled; no significant genome rearrangements between the cell forms were observed, but the relative abundances of replicons were different. GANTC, GGCGCC, and GATC methylated motifs were found in the genome, along with genes encoding methyltransferases with matching predicted target motifs. The GGCGCC motif was completely methylated in both states, with two restriction–modification clusters on different replicons enforcing this specific pattern of methylation. Methylation patterns for the GANTC and GATC motifs differed significantly depending on the cell state, which indicates their possible connection to the regulation of symbiotic differentiation. Further investigation into the differences of methylation patterns in the bacterial genomes coupled with gene expression analysis is needed to elucidate the function of bacterial epigenetic regulation in nitrogen-fixing symbiosis.

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