Enteric and non-enteric adenoviruses associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017

Aim: The aim of the present study was to detect the prevalence of norovirus and genotypes determination by real-time PCR among children below 18 years as an etiology of acute gastroenteritis and to compare rapid detection of norovirus by Enzyme-Linked Immunoassay (ELISA) to virus detection by real-time PCR. Methods: The research was a cross-sectional study conducted on children below 18 years complaining of community-acquired acute gastroenteritis. A stool sample was subjected to direct-antigen detection by ELISA for norovirus and molecular study by real-time polymerase chain reaction. Results: The study included 200 children with acute gastroenteritis with a mean age of 6.7±3.8 years. Norovirus antigen was detected by EIA in 34.5% and by real-time PCR in 30.5% of studied children with genotype GII, the predominant detected genotype (80.97%). Both real-time PCR and antigen detection of norovirus were positive in 43 (70.5%) of the children and negative in 113(81.3%) of the studied children. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy for antigen detection by ELISA were 70.5%, 81.3%, 62.3%, 86.3% and 78%, respectively. Comparison between patients positive for norovirus and those negative for norovirus by real-time PCR revealed non-significant difference as regards age, sex, the season of occurrence and residence. Conclusion: The present study highlights that norovirus prevalence is common among pediatric patients with gastroenteritis above 5 years with GII genotype as the prevalent genotype. There was a significant correlation between positive and negative results of antigen detection of norovirus by ELISA and detection of RNA of norovirus by real-time PCR in stool samples. However, the screening for norovirus by ELISA has limited sensitivity and needs to be associated with a molecular method for accurate diagnosis of sporadic cases of gastroenteritis.

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The incidence of rotavirus infection compared to bacterial infections in different age groups of pediatric patients with gastroenteritis

Sanitarno inženirstvo International Journal of Sanitary Engineering Research ◽

10.2478/ijser-2019-0005 ◽

2019 ◽

Vol 13 (1) ◽

pp. 39-52

Author(s):

Tatjana Babić ◽

Biljana Miljković-Selimović ◽

Dobrila Đorđević-Stanković ◽

Branislava Kocić ◽

Miloš Ranđelović ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Bacterial Infections ◽

Pediatric Patients ◽

Age Groups ◽

Outpatient Setting ◽

Diarrheal Diseases ◽

Rotavirus Infection ◽

Identification Of Bacteria ◽

One Year ◽

The Town

AbstractRotavirus is the important cause of acute gastroenteritis in pediatric patients. The aim of the present research was to determine the incidence of rotavirus infections in infants and children up to seven years of age in the town of Niš. Seasonal prevalence of rotavirus-associated acute gastroenteritis was also evaluated. An enzyme immunoassay (RIDASCREEN® Rotavirus; R-Biopharm AG, Darmstadt, Germany) was used to detect rotavirus in the stool specimens of 1,156 patients (newborns up to 7 years of age) presenting with gastroenteritis. Identification of bacteria and yeasts was performed by classical methods. The overall incidence of rotavirus in examined children was 5.97%. Among 144 hospitalized children, rotavirus infection was diagnosed in 28 (19.44%). In 1,012 children treated in outpatient setting for diarrheal diseases, rotaviruses were found in 41 (4.05%). The highest incidence of rotavirus infection was among the patients of one year of age. Among 1,156 pediatric children tested, bacterial pathogens were found in 6.31% and the most frequently isolated pathogens were Campylobacter spp. and Salmonella enteritidis. The highest prevalence of GE was recorded in the colder season, peaking in April (15.94%). Rotaviruses are an important factor in the etiology of the acute diarrheal diseases, especially in children hospitalized during the winter/spring season.

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