scholarly journals Point prevalence of asymptomatic Plasmodium infection and the comparison of microscopy, rapid diagnostic test and nested PCR for the diagnosis of asymptomatic malaria among children under 5 years in Ghana

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0232874
Author(s):  
Bismark Okyere ◽  
Alex Owusu-Ofori ◽  
Daniel Ansong ◽  
Rebecca Buxton ◽  
Scott Benson ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Nested Pcr ◽  
Point Prevalence ◽  
Asymptomatic Malaria ◽  
Plasmodium Infection ◽  
Children Under 5

scholarly journals Performance and usefulness of the Hexagon rapid diagnostic test in children with asymptomatic malaria living in the Mount Cameroon region

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Samuel Wanji ◽  
Helen K Kimbi ◽  
Joan E Eyong ◽  
Nicholas Tendongfor ◽  
Judith L Ndamukong
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Asymptomatic Malaria ◽  
Mount Cameroon

scholarly journals Malaria Surveillance in the Anak Dalam Tribe, Jambi, Indonesia

2021 ◽  
Vol 12 (2) ◽  
pp. 104-116
Author(s):  
Ade Suryaman ◽  
Chairil Anwar ◽  
Dwi Handayani ◽  
Irsan Saleh ◽  
Dalillah Dalillah ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Malaria Incidence ◽  
Diagnostic Tools ◽  
Asymptomatic Malaria ◽  
Patient Identification ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Transmission of malaria is common in countries with tropical and subtropical. In several cases, malaria presents in the sufferer especially in individuals that have never received treatment shortly. As the early phase of patient identification, screening is important as an effort to eradicate malaria, in addition to the gold standard which currently used, other diagnostic tools are needed to conduct a malaria incidence survey to obtain comparative data using 3 diagnostic tools, goal standard checks, which are currently unable to detect asymptomatic malaria. This study aims to compare three standard diagnostic test (Rapid Diagnostic Test, Giemsa, and Nested Polymerase Chain Reaction) for the measurement of malaria prevalence within the Anak Dalam Tribe community in Jambi, Indonesia. This descriptive research with a survey design was conducted on 99 persons in Anak Dalam Tribe. It was discovered that majority of the respondents were the adult age category (52.5%),were female (50.5%). The prevalence of this infectious disease as detected by Rapid Diagnostic Test, microscopy, and Nested Polymeras Chain Reaction examination were 3%, 5% and 35.3% respectively. Non-Plasmodium falciparum was detected by examining samples with Rapid Diagnostic Test, on microscope, P. vivax and P. falciparum were found, while on Nested Polymerase Chain Reaction, two bands at 120bp and 205bp showed P. vivax and P. falciparum respectively. Nested Polymeras Chain Reaction is more sensitive to detected asymptomatic malaria, however, Rapid Diagnostic Test and Giemsa sensitive with parasitemia threshold.

scholarly journals Usefulness of rapid diagnostic test in the diagnosis of asymptomatic malaria in HIV infected children on cotrimoxazole prophylaxis in Benin City, Nigeria

2020 ◽  
Vol 47 (4) ◽  
pp. 312-317
Author(s):  
M.O. Ibadin ◽  
N. Eghafona
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Opportunistic Infections ◽  
Asymptomatic Malaria ◽  
Malaria Parasitaemia ◽  
Cross Sectional ◽  
Parasite Count ◽  
Benin City ◽  
Cotrimoxazole Prophylaxis

Background: Rapid diagnostic test (mRDT) is a useful tool in demonstrating parasitologically proven malaria. Its efficacy is however hampered  when parasite density is low. Prophylactic use of cotrimoxazoleas in cases of HIV infected children can cause reduction in parasite count. It is  doubtful if mRDT will retain its diagnostic usefulness among such individuals.Objectives: The study sought to evaluate the diagnostic value of mRDT in HIV infected children on cotrimoxazole prophylaxis in Benin City.Methods: In the prospective, cross sectional and descriptive study, we assessed malaria parasitaemia using standard methods in microscopy and parasite density and malaria antigenaemia using Care Start Pf (monoclonal antibodies specific to histidine rich protein – 2 antigen) in 221 each of HIV infected subjects on cotrimoxazole managed in a specialist clinic and HIV negative controls all seen at the University of Benin Teaching Hospital between April and June 2016.Results: Malaria antigenaemia rate MAr (20.8%) was lower than malaria parasitaemia rate MPr (24.4%) in subjects. MAr (20.8) and MPr (24.4%) in  subjects were higher than MAr (18.10%) and MPr (17.7%) in controls. Mean (SEM) parasite count in subjects of was low (50.88 + 2.24 per μl). Using microscopy as gold standard the sensitivity, specificity, PPV and NPV of mRDT in subjects were 77.8%, 97.6%, 91.3% and 93.1%. Corresponding values in controls were 100.0%, 99.5%, 97.5% and 100.0%.Youden indices for subjects and controls were 0.75 and 0.99.Conclusions/Recommendations: Sensitivity of mRDT in HIV infected children on cotrimoxazole prophylaxis for opportunistic infections (OI) is reduced. However the indices of specificity, PPV and NPV are high enough to retain its value in the evaluation of HIV infected children for  asymptomatic malaria and perhaps the clinical disease. Keywords: mRDT, Utility, HIVinfected Children, Cotrimoxazoleprophylaxis, Benin City 

Performance of a new gelled nested PCR test for the diagnosis of imported malaria: comparison with microscopy, rapid diagnostic test, and real-time PCR

2014 ◽  
Vol 113 (7) ◽  
pp. 2587-2591 ◽  
Author(s):  
Nuria Iglesias ◽  
Mercedes Subirats ◽  
Patricia Trevisi ◽  
Germán Ramírez-Olivencia ◽  
Pablo Castán ◽  
...  
Keyword(s):  
Real Time ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Imported Malaria ◽  
Pcr Test

scholarly journals Sensitivity and Specificity of Rapid Diagnostic Test with Microscopic Gold Standard to Identify Plasmodium Species

2016 ◽  
Vol 5 (4) ◽  
pp. 354
Author(s):  
Sahrir Sillehu ◽  
Heny Arwati ◽  
Yoes Prijatna Dachlan ◽  
Sudjajadi Keman
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Predictive Value ◽  
Gold Standard ◽  
Plasmodium Species ◽  
Malaria Diagnosis ◽  
Observational Research ◽  
Asymptomatic Malaria ◽  
Standard Result ◽  
A Value

Malaria is a main health problem in islands area which is under developed and isolated. Nation-wide, in 2014 Maluku province was recorded to have Annual Malaria Incident (AMI) value of 30.4%, positive incidents of 13.30%, ABER 3.76%, SPR 21.50%, and Annual Paracite Incident (API) 8.10%, while South Buru Regency has a value of Annual Malaria Incident (AMI) of 14.49%, 494 positive incidents, ABER 1.12%, SPR 60.91%, and Annual Paracite Incident (API) 6.86%. The purpose of this study was to identify Plasmodium species in malaria incidents in NamroleSubdistrict, South Buru Regency, Maluku Province. Observational research with a sample of 64 respondents for symptomatic and asymptomatic malaria. The instrument for the research was Rapid Diagnostic Test (RDT) and microscopic Gold Standard. Result: Malaria examination by using RDT suggested 3 kinds of parasites, i.e., P. falciparum, P. Vivax, and a mix between P. falciparum and P. vivax. Most parasites found were P. falciparum 56.3%. The accuracy of RDT examination was proven with microscopic test and the result suggested that the RDT sensitivity was 100% and the specifivity was 63.3%. Positive predictive value was 92.9% and negative predictive value was 100%, both were for positive likelihood ration of 2.75%. While for negative likelihood ration of 0%, the value of degree of conformity (Kappa) between RDT and microscopic is 0%. RDT has one benefit that it can be use to conduct malaria diagnosis rapidly, particularly in isolated areas. The benefit of Rapid Diagnostic Test (RDT) was that it could be used in remote and isolated areas to conduct diagnosis. RDT is highly effective and efficient.

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