scholarly journals Usefulness of rapid diagnostic test in the diagnosis of asymptomatic malaria in HIV infected children on cotrimoxazole prophylaxis in Benin City, Nigeria

2020 ◽  
Vol 47 (4) ◽  
pp. 312-317
Author(s):  
M.O. Ibadin ◽  
N. Eghafona
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Opportunistic Infections ◽  
Asymptomatic Malaria ◽  
Malaria Parasitaemia ◽  
Cross Sectional ◽  
Parasite Count ◽  
Benin City ◽  
Cotrimoxazole Prophylaxis

Background: Rapid diagnostic test (mRDT) is a useful tool in demonstrating parasitologically proven malaria. Its efficacy is however hampered  when parasite density is low. Prophylactic use of cotrimoxazoleas in cases of HIV infected children can cause reduction in parasite count. It is  doubtful if mRDT will retain its diagnostic usefulness among such individuals.Objectives: The study sought to evaluate the diagnostic value of mRDT in HIV infected children on cotrimoxazole prophylaxis in Benin City.Methods: In the prospective, cross sectional and descriptive study, we assessed malaria parasitaemia using standard methods in microscopy and parasite density and malaria antigenaemia using Care Start Pf (monoclonal antibodies specific to histidine rich protein – 2 antigen) in 221 each of HIV infected subjects on cotrimoxazole managed in a specialist clinic and HIV negative controls all seen at the University of Benin Teaching Hospital between April and June 2016.Results: Malaria antigenaemia rate MAr (20.8%) was lower than malaria parasitaemia rate MPr (24.4%) in subjects. MAr (20.8) and MPr (24.4%) in  subjects were higher than MAr (18.10%) and MPr (17.7%) in controls. Mean (SEM) parasite count in subjects of was low (50.88 + 2.24 per μl). Using microscopy as gold standard the sensitivity, specificity, PPV and NPV of mRDT in subjects were 77.8%, 97.6%, 91.3% and 93.1%. Corresponding values in controls were 100.0%, 99.5%, 97.5% and 100.0%.Youden indices for subjects and controls were 0.75 and 0.99.Conclusions/Recommendations: Sensitivity of mRDT in HIV infected children on cotrimoxazole prophylaxis for opportunistic infections (OI) is reduced. However the indices of specificity, PPV and NPV are high enough to retain its value in the evaluation of HIV infected children for  asymptomatic malaria and perhaps the clinical disease. Keywords: mRDT, Utility, HIVinfected Children, Cotrimoxazoleprophylaxis, Benin City 

scholarly journals Evaluation of a Rapid Diagnostic Test Currently Being Used in the Management of Malaria in Patients of the Hia/Chu-Parakou and the Boko Zone Hospital in Northern Benin

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Tokponnon F ◽  
◽  
Osse R ◽  
Houessou C ◽  
Akogbeto M ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Probabilistic Method ◽  
Health Centers ◽  
Patient Identification ◽  
Cross Sectional ◽  
Predictive Values ◽  
Parasitological Diagnosis ◽  
Sensitivity Specificity ◽  
Adequate Management

Parasitological diagnosis is a fundamental element in the adequate management of the disease. In the last decade, there has been a resurgence of interest in the development of malaria Rapid Diagnostic Test (RDT) kits. This is the case with SD Bioline Malaria Ag Pf, which searches for HRP2 antigen by immunochromatography. The objective of this study is to compare the results of RDT SD Bioline Malaria Ag Pf in use with the results of Thick Gout (TG) in the biological diagnosis of malaria. This was a cross-sectional, descriptive and evaluative study carried out at the Hôpital d’Instruction des Armées-Center Hospitalier et Universitaire de Parakou and at the Boko zone hospital from April 20 to July 30, 2015. Patient identification, we used the non-probabilistic method and the convenience choice technique. The study involved 503 patients. The results of this study showed a good performance of the RDT SD Bioline Malaria Ag Pf. Among the 503 patients, 199 or 39.6% were positive for the RDT against 180 or 35.8% positive for the Thick Gout (TG). Sensitivity, specificity, positive and negative predictive values of the test compared to the thick film were respectively (91.7%, 89.5%, 82.9, 95) and the Kappa coefficient of 0.88 testifies a good match. False positive cases are noted in patients on treatment and even after recovery due to the persistence of the HRP2 antigen in the blood. However, it constitutes an interesting alternative to the management of malaria. At the end of this study, we suggest continuing the use of RDTs in health centers where microscopy is absent and/or reinforcing microscopy, and to strengthen staff training in the management of malaria cases.

scholarly journals Rapid Diagnostic Test Versus Microscopy for Diagnosing Malaria Among Pregnant Women in a Resource-Poor Setting; A Cross-Sectional Comparative Study

2020 ◽  
Vol 12 (8) ◽  
pp. 52
Author(s):  
Bartholomew N. Odio ◽  
Leonard O. Ajah ◽  
Perpetus C. Ibekwe ◽  
Monique I. Ajah ◽  
George O. Ugwu ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Clinical Features ◽  
Scale Up ◽  
World Health ◽  
Resource Poor Setting ◽  
Cross Sectional ◽  
Significant Difference ◽  
Resource Poor

BACKGROUND: Diagnostic challenge of malaria in Nigeria remarkably impedes the World Health Organization (WHO) recommendation of laboratory diagnosis before treatment. Rapid Diagnostic Test (RDT) is easier and cheaper to perform when compared with microscopy especially in resource-poor settings. However there are conflicting results on the accuracy of RDT versus microscopy from previous studies. AIM: To compare the overall accuracy of   microscopy and RDT in detecting peripheral malaria among   pregnant women with clinical features of malaria. MATERIALS & METHODS: This was a cross-sectional comparative studyin whichRDT, microscopy and polymerase chain reaction (PCR) were performed using the peripheral bloodof the eligible study participants at the Alex Ekwueme Federal University Teaching Hospital, Abakaliki between September 1, 2016 and March 31, 2017.The PCR was used as the gold standard in this study. Data was analyzed with the Statistical Package for Social Sciences version 18 (IBM SPSS, Chicago, USA). P value ≤ 0.05 was considered statistically significant. RESULTS: The actual prevalent rates of malaria based on RDT, microscopy and PCR results among the participants were 58.2%, 59.9% and 61.1% respectively. There was no statistical significant difference among RDT, microscopy and combined RDT and microscopy on overall accuracy. Malaria infestation was associated with self-employed and unemployed women, primigravidity, second trimester, rural residence, non-use of long lasting insecticide treated nets and intermittent preventive therapy for malaria. CONCLUSION: There was no difference in overall accuracy among RDT, microscopy and combined RDT and microscopy. This underscores the need to scale up RDT for every patient with clinical features of malaria before treatment in this environment.

scholarly journals Comparative Study of Malaria Diagnosis: Rapid Diagnostic Test (RDTs) Against Microscopy among Pregnant Women Attending Antenatal Clinic in Specialist Hospital, Sokoto

2021 ◽  
pp. 9-16
Author(s):  
K. Mohammed ◽  
M. U. Iduh ◽  
S. M. Saheed ◽  
O. F. Ashcroft ◽  
M. K. Garba ◽  
...  
Keyword(s):  
Comparative Study ◽  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Study Design ◽  
Gold Standard ◽  
Malaria Parasite ◽  
Malaria Diagnosis ◽  
Sub Saharan Africa ◽  
Cross Sectional

Background: Malaria is an infectious disease caused by a protozoan parasite of the genus Plasmodium. It was estimated that 219 million cases of malaria occur in 87 countries, with an estimated death of 435,000 in 2017 among pregnant women. Other species include P. ovale, P. vivax, and P. malariae is a blood parasite of human and is one of the major public health burdens in developing countries, particularly in Sub-Saharan Africa. It is estimated that about 3.5 billion people globally and 450 million people are thought to be ill as a result of such infections, the majority being children. Aims: This study was aimed at comparing RDTs against microscopy in the detection of malaria parasite among pregnant women. Samples were collected and analyzed following cross-sectional comparative study design. It was conducted between Septembers to November 2019. Study Design: This was a cross-sectional, comparative study Place and Duration of Study: This study was conducted among patients attending Specialist Hospital Sokoto, Sokoto State, between March and November, 2019. Methodology: A total of 106 participants were enrolled for the study. Standard parasitological examination was carried out on blood samples using microscopy followed by Rapid Diagnostic test (RDTs). Results: Finding revealed, in this study, CareStart kit had sensitivity of 77.7%, specificity of 100s%. It is expected that any RDT used for malaria diagnosis should have a high sensitivity of 95% and specificity 97% (WHO, 2003); this is in contrast with the RDTs results in this study. The false negative (FN) Carestart and SD-Bioline kits in this study were 10% and 21%, respectively using microscopy as the gold standard due to lack of sensitivity of RDTs at low parsitaemia compared to microscopy. The false positive rate in this study for the Carestart and SD-Bioline kits are 0% and 0% respectively. Out of 106 patients screened, 35% and 24% tested positive for Plasmodiumfalciparum using Carestart and SD-Bioline RDTs respectively, while 45% were positive to malaria by microscopic examination. There was high prevalence of malaria parasite among age group 18-23 which is 42.2%. Conclusion: It can be concluded that using microscopy is more time consuming compared to RDTs due to the fact that the time taken to read the results for RDTs is within five minutes and that of microscopy is higher compared to RDTs. Over all prevalence of malaria by microscopy was 42.5% while the prevalence of malaria by Carestart and SD-bioline was 33% and 22.6% respectively. Conventional microscopy remains the gold standard compared to RDTs according to this study with the level of its sensitivity and specificity which is higher than RDTs.

scholarly journals Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Valérie Briand ◽  
Gilles Cottrell ◽  
Nicaise Tuike Ndam ◽  
Xavier Martiáñez-Vendrell ◽  
Bertin Vianou ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Quantitative Polymerase Chain Reaction ◽  
Suspension Array ◽  
Increased Risk ◽  
Array Technology ◽  
Highly Sensitive ◽  
Polymerase Chain

Abstract Background While sub-microscopic malarial infections are frequent and potentially deleterious during pregnancy, routine molecular detection is still not feasible. This study aimed to assess the performance of a Histidine Rich Protein 2 (HRP2)-based ultrasensitive rapid diagnostic test (uRDT, Alere Malaria Ag Pf) for the detection of infections of low parasite density in pregnant women. Methods This was a retrospective study based on samples collected in Benin from 2014 to 2017. A total of 942 whole blood samples collected in 327 women in the 1st and 3rd trimesters and at delivery were tested by uRDT, conventional RDT (cRDT, SD BIOLINE Malaria Ag Pf), microscopy, quantitative polymerase chain-reaction (qPCR) and Luminex-based suspension array technology targeting P. falciparum HRP2. The performance of each RDT was evaluated using qPCR as reference standard. The association between infections detected by uRDT, but not by cRDT, with poor maternal and birth outcomes was assessed using multivariate regression models. Results The overall positivity rate detected by cRDT, uRDT, and qPCR was 11.6% (109/942), 16.2% (153/942) and 18.3% (172/942), respectively. Out of 172 qPCR-positive samples, 68 were uRDT-negative. uRDT had a significantly better sensitivity (60.5% [52.7–67.8]) than cRDT (44.2% [36.6–51.9]) and a marginally decreased specificity (93.6% [91.7–95.3] versus 95.7% [94.0–97.0]). The gain in sensitivity was particularly high (33%) and statistically significant in the 1st trimester. Only 28 (41%) out of the 68 samples which were qPCR-positive, but uRDT-negative had detectable but very low levels of HRP2 (191 ng/mL). Infections that were detected by uRDT but not by cRDT were associated with a 3.4-times (95%CI 1.29–9.19) increased risk of anaemia during pregnancy. Conclusions This study demonstrates the higher performance of uRDT, as compared to cRDTs, to detect low parasite density P. falciparum infections during pregnancy, particularly in the 1st trimester. uRDT allowed the detection of infections associated with maternal anaemia.

scholarly journals Performance and usefulness of the Hexagon rapid diagnostic test in children with asymptomatic malaria living in the Mount Cameroon region

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Samuel Wanji ◽  
Helen K Kimbi ◽  
Joan E Eyong ◽  
Nicholas Tendongfor ◽  
Judith L Ndamukong
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Asymptomatic Malaria ◽  
Mount Cameroon

scholarly journals Uji Diagnostik Filariasis Menggunakan Rapid Diagnostic Test (RDT) Brugia malayi terhadap Pemeriksaan Mikroskopis di Desa Buntoi Kabupaten Gunung Mas, Provinsi Kalimantan Tengah

2021 ◽  
pp. 107-118
Author(s):  
Gadis Rinaty Susanty ◽  
Hernayanti Hernayanti ◽  
Dwi Sarwani Sri Rejeki
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Microscopic Examination ◽  
Predictive Value ◽  
Diagnostic Technique ◽  
Brugia Malayi ◽  
Diagnostic Study ◽  
Research Subjects ◽  
Cross Sectional ◽  
Central Kalimantan

Gunung Mas Regency, Central Kalimantan Province is one of the endemic filariasis areas with Microfilaria rate of 3.4%. One of the efforts made to control this problem is Mass Drug Administration once a year for 5 years. Currently, the Rapid Diagnostic Test (RDT) method is being developed, a quick and easy diagnostic technique to detect the presence of parasites in the patient's body. This study aims to determine the results of the filariasis diagnostic test using the Brugia malayi RDT on the microscopic examination in Buntoi Village, Gunung Mas Regency, Central Kalimantan Province. This research is a descriptive study with a cross-sectional approach with the research subjects all residents of Buntoi Village with inclusion and exclusion criteria totaling 161 samples. Collecting data was carried out by examination and interviews with questionnaires. Data analysis by calculating the microfilaria rate, sensitivity and specificity and calculating the frequency distribution of research variables. Data is presented in percentage form and displayed in tabular form. The results of the diagnostic study of B. malayi RDT and the microscopic examination were the same, i.e all were negative and no microfilariae were found. The diagnostic test for filariasis RDT Brugia malayi  on microscopic examination (SDJ) obtained 0% sensitivity, 100% specificity, 0% Positive Predictive Value and 100% Negative Predictive Value. The level of public knowledge about filariasis includes 61% good category, knowledge of MDA 40% good category and knowledge about prevention of filariasis in good category 53%.

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