scholarly journals Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258266
Author(s):  
Sujin Baek ◽  
Hyun Ho Noh ◽  
Chang Jo Kim ◽  
Kyungae Son ◽  
Hee-Dong Lee ◽  
...  
Keyword(s):  
Analytical Method ◽  
Optimal Recovery ◽  
Mushroom Compost ◽  
Spent Mushroom Compost ◽  
Safety Standards ◽  
Relative Standard ◽  
Liquid Chromatography Tandem Mass ◽  
Simple Extraction ◽  
Antithrombotic Efficacy ◽  
Protaetia Brevitarsis Seulensis

Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contaminated with three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the insect. This study was aimed to optimize a simple extraction method combined with liquid chromatography tandem mass spectrometry and apply it to the real samples. After the pulverized samples (5 g) were extracted with acetonitrile (10 mL) and formic acid (100 μL), fat and lipids in the samples were slowly precipitated at -20°C for 24 hours. After eight different clean-up methods were investigated, the mixture of 150 mg MgSO4/25 mg PSA/25 mg C18 was selected due to optimal recovery of the target compounds. Recovery (77.9%‒80.8% for carbendazim, 111.2%‒116.7% for dimethomorph, and 111.9%‒112.5% for fenoxanil) was achieved with reasonable relative standard deviation (<5.5%) The analytical method developed in this study was used to analyze three compounds in the 24 insect samples donated by the insect farm owners but no target compounds were detected. These results can provide important data for establishing the pesticide safety standards for P. brevitarsis before the medical applications.

scholarly journals Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Haiyan Lei ◽  
Jianbo Guo ◽  
Zhuo Lv ◽  
Xiaohong Zhu ◽  
Xiaofeng Xue ◽  
...  
Keyword(s):  
Recovery Rates ◽  
Inhibition Effect ◽  
Standard Curve ◽  
Modified Quechers ◽  
Relative Standard ◽  
Acacia Honey ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass ◽  
Standard Deviations

This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey.

scholarly journals Determination of 77 Multiclass Pesticides and Their Metabolitesin Capsicum and Tomato Using GC-MS/MS and LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1837
Author(s):  
Harischandra Naik Rathod ◽  
Bheemanna Mallappa ◽  
Pallavi Malenahalli Sidramappa ◽  
Chandra Sekhara Reddy Vennapusa ◽  
Pavankumar Kamin ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Solid Phase ◽  
Graphitized Carbon Black ◽  
Limit Of Quantification ◽  
Gas And Liquid Chromatography ◽  
Relative Standard ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass ◽  
Primary Secondary Amine

A quick, sensitive, and reproducible analytical method for the determination of 77 multiclass pesticides and their metabolites in Capsicum and tomato by gas and liquid chromatography tandem mass spectrometry was standardized and validated. The limit of detection of 0.19 to 10.91 and limit of quantification of 0.63 to 36.34 µg·kg−1 for Capsicum and 0.10 to 9.55 µg·kg−1 (LOD) and 0.35 to 33.43 µg·kg−1 (LOQ) for tomato. The method involves extraction of sample with acetonitrile, purification by dispersive solid phase extraction using primary secondary amine and graphitized carbon black. The recoveries of all pesticides were in the range of 75 to 110% with a relative standard deviation of less than 20%. Similarly, the method precision was evaluated interms of repeatability (RSDr) and reproducibility (RSDwR) by spiking of mixed pesticides standards at 100 µg·kg−1 recorded anRSD of less than 20%. The matrix effect was acceptable and no significant variation was observed in both the matrices except for few pesticides. The estimated measurement uncertainty found acceptable for all the pesticides. This method found suitable for analysis of vegetable samples drawn from market and farm gates.

Ensuring social guarantees and human rights for the implementation of the labour legislation of the Republic of Kazakhstan

2020 ◽  
pp. 315-335
Author(s):  
Anarkhan R. Kuttygalieva ◽  
Yermek A. Buribayev ◽  
Bakhytkali M. Koshpenbetov ◽  
Gakku N. Rakhimova ◽  
Zhanna A. Khamzina ◽  
...  
Keyword(s):  
Human Rights ◽  
Labour Market ◽  
Analytical Method ◽  
Practical Significance ◽  
Safety Standards ◽  
Collective Agreements ◽  
The Republic ◽  
Labour Legislation ◽  
And Control ◽  
Labour Regulation

The relevance of the study is determined by the fact that in order to improve the legislation of the country, it is necessary to study the successful experience of other countries. The purpose of the study is to propose a number of measures that will help improve labour legislation in Kazakhstan. The work uses primarily the analytical method. It is determined by the fact that apart from statutory labour protection rules, some safety standards are contained in collective agreements that speci-fy and sometimes supplement relevant legislative provisions. The paper states the fact that it is necessary to factor in world experience in organizing and operating a system of supervision and control over compliance with labour legislation for the Republic of Kazakhstan. The novelty of the study is that with a study of the legislation on labour regulation in Western countries, the authors identify a number of measures that will be relevant for implementation in the Republic of Kazakhstan. The practical significance of the study is determined by the incompatibility of iso-lation from the outside world, including from the international labour market with objectives of development.

Nematicidal activity of the Lentinula edodes’ spent mushroom compost

2022 ◽  
Vol 146 ◽  
pp. 101-102
Author(s):  
Juliana Marques Ferreira ◽  
Fabio Ribeiro Braga ◽  
Filippe Elias de Freitas Soares
Keyword(s):  
Lentinula Edodes ◽  
Nematicidal Activity ◽  
Mushroom Compost ◽  
Spent Mushroom Compost

scholarly journals Effects of spent mushroom compost meal on growth performance and meat characteristics of grower geese

2016 ◽  
Vol 45 (6) ◽  
pp. 281-287 ◽  
Author(s):  
Shen-Chang Chang ◽  
Min-Jung Lin ◽  
Yun-Peng Chao ◽  
Chung-Jen Chiang ◽  
Yu-Shine Jea ◽  
...  
Keyword(s):  
Growth Performance ◽  
Mushroom Compost ◽  
Spent Mushroom Compost

scholarly journals Spent Mushroom Compost as a Substrate for the Production of Lettuce Seedlings

2014 ◽  
Vol 6 (7) ◽  
Author(s):  
E. L. S. Marques ◽  
E. T. Martos ◽  
R. J. Souza ◽  
R. Silva ◽  
D. C. Zied ◽  
...  
Keyword(s):  
Mushroom Compost ◽  
Spent Mushroom Compost

scholarly journals Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

2015 ◽  
Vol 51 (4) ◽  
pp. 823-832 ◽  
Author(s):  
Francine Rodrigues Ianiski ◽  
Luciane Varini Laporta ◽  
Alexandre Machado Rubim ◽  
Cristiane Luchese
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Zeta Potential ◽  
Analytical Method ◽  
High Performance ◽  
Ph Value ◽  
Hplc Method ◽  
Polydispersity Index ◽  
Size Distributions ◽  
Relative Standard

abstract A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed HPLC method provides selective, linear results in the range of 1.0-40.0 μg/mL; quantification and detection limits were 1.78 μg/mL and 0.59 μg/mL, respectively; relative standard deviation for repeatability was 1.35% and intermediate precision was 0.41% and 0.61% for analyst 1 and analyst 2, respectively; accuracy between 99.23 and 101.79%; robustness between 97.13 and 98.45% for the quantification of M-PEGNC. Mean particle diameters were 261 ± 13 nm and 249 ± 20 nm, polydispersity index was 0.15 ± 0.07 and 0.17 ± 0.06, pH values were 5.0 ± 0.2 and 5.2 ± 0.1, and zeta-potential values were -37.9 ± 3.2 mV e -31.8 ± 2.8 mV for M-PEGNC and placebo(B-PEGNC), respectively. In conclusion, the proposed analytical method is suitable for the quality control of M-PEGNC. Moreover, suspensions showed monomodal size distributions and low polydispersity index indicating high homogeneity of formulations with narrow size distributions, and appropriate pH and zeta potential. The extraction process was efficient for release of meloxicam from nanostructured systems.

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