Oviposition activity of Haemagogus leucocelaenus (Diptera: Culicidae) during the rainy and dry seasons, in areas with yellow fever virus circulation in the Atlantic Forest, Rio de Janeiro, Brazil

The present study aims to analyze the effectiveness of ovitraps in the capture of Hg leucocelaenus eggs and evaluate the influence of the dry and rainy seasons on their abundance and hatching rates. The eggs were collected in the Atlantic Forest of State of Rio de Janeiro, Brazil, an area in which the yellow fever virus is known to circulate. We distributed 15 ovitraps in three sampling points, with five ovitraps per point. We distributed 15 ovitraps in three sampling points on trees within a forested area, which were sequentially numbered, monitored, and replaced every two weeks from October 2016 to April 2018. There was a high dominance of Hg. leucocelaenus eggs (98.4%) and a variation in egg hatching rates between the wet and dry seasons. These rates were 1.5 times higher in the rainy season than in the dry season. The rainy season also showed a greater abundance of eggs and higher values of ovitrap positivity and egg density indexes in the installed ovitraps. The abundances of Hg. leucocelaenus eggs were positively correlated with mean monthly temperature and air humidity but not significantly correlated with accumulated precipitation. These results, as well as their implications for the possible use of ovitraps to monitor vector mosquitoes of yellow fever in the study region, are discussed.

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Yellow Fever Virus Reemergence and Spread in Southeast Brazil, 2016–2019

Journal of Virology ◽

10.1128/jvi.01623-19 ◽

2019 ◽

Vol 94 (1) ◽

Cited By ~ 8

Author(s):

Marta Giovanetti ◽

Marcos Cesar Lima de Mendonça ◽

Vagner Fonseca ◽

Maria Angélica Mares-Guia ◽

Allison Fabri ◽

...

Keyword(s):

Genetic Diversity ◽

Yellow Fever ◽

Rio De Janeiro ◽

Nonhuman Primates ◽

Yellow Fever Virus ◽

Genomic Data ◽

Fever Virus ◽

Southeastern Brazil ◽

Espirito Santo ◽

Southeastern Region

ABSTRACT The recent reemergence of yellow fever virus (YFV) in Brazil has raised serious concerns due to the rapid dissemination of the virus in the southeastern region. To better understand YFV genetic diversity and dynamics during the recent outbreak in southeastern Brazil, we generated 18 complete and nearly complete genomes from the peak of the epidemic curve from nonhuman primates (NHPs) and human infected cases across the Espírito Santo and Rio de Janeiro states. Genomic sequencing of 18 YFV genomes revealed the estimated timing, source, and likely routes of yellow fever virus transmission and dispersion during one of the largest outbreaks ever registered in Brazil. We showed that during the recent epidemic, YFV was reintroduced from Minas Gerais to the Espírito Santo and Rio de Janeiro states multiple times between 2016 and 2019. The analysis of data from portable sequencing could identify the corridor of spread of YFV. These findings reinforce the idea that continued genomic surveillance strategies can provide information on virus genetic diversity and transmission dynamics that might assist in understanding arbovirus epidemics. IMPORTANCE Arbovirus infections in Brazil, including yellow fever, dengue, zika, and chikungunya, result in considerable morbidity and mortality and are pressing public health concerns. However, our understanding of these outbreaks is hampered by the limited availability of genomic data. In this study, we investigated the genetic diversity and spatial distribution of YFV during the current outbreak by analyzing genomic data from areas in southeastern Brazil not covered by other previous studies. To gain insights into the routes of YFV introduction and dispersion, we tracked the virus by sequencing YFV genomes sampled from nonhuman primates and infected patients from the southeastern region. Our study provides an understanding of how YFV initiates transmission in new Brazilian regions and illustrates that genomics in the field can augment traditional approaches to infectious disease surveillance and control.

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Evaluation of the Diversity of Culicidae Vectors of the Sylvatic Yellow Fever Virus in Atlantic Forest Remnants with the Use of Ovitraps and Bamboo Traps

Vector-Borne and Zoonotic Diseases ◽

10.1089/vbz.2021.0033 ◽

2021 ◽

Author(s):

Alexandre de Araujo-Oliveira ◽

Hélcio Reinaldo Gil-Santana ◽

Carolina de Souza Brandão Teixeira ◽

Jacenir Reis dos Santos-Mallet ◽

Jeronimo Alencar

Keyword(s):

Atlantic Forest ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus ◽

Forest Remnants ◽

Bamboo Traps

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Yellow fever virus spread in Rio de Janeiro and Espírito Santo, 2016-2019: Phylodynamic assessment to improve intervention strategies

10.1101/711994 ◽

2019 ◽

Cited By ~ 1

Author(s):

Marta Giovanetti ◽

Marcos Cesar Lima de Mendonça ◽

Vagner Fonseca ◽

Maria Angélica Mares-Guia ◽

Allison Fabri ◽

...

Keyword(s):

Public Health ◽

Real Time ◽

Yellow Fever ◽

Rio De Janeiro ◽

Yellow Fever Virus ◽

Fever Virus ◽

Southeastern Brazil ◽

Espírito Santo ◽

Espirito Santo ◽

Southeastern Region

ABSTRACTThe recent re-emergence of yellow fever virus (YFV) in Brazil has raised serious concerns due to the virus’ rapid dissemination in the southeastern region. To better understand YFV genetic diversity and dynamics during the recent outbreak in southeastern Brazil we generated 18 complete and near-complete genomes from the peak of the epidemic curve from non-human primates (NHPs) and human infected cases across Espírito Santo and Rio de Janeiro states. Genomic sequencing of 18 YFV genomes revealed the timing, source and likely routes of yellow fever virus transmission and dispersion during the one of the largest outbreaks ever registered in Brazil. We showed that the recent YFV epidemic spillover southwards several times from Minas Gerais to Espírito Santo and Rio de Janeiro states in 2016 to 2019. The quick production and analysis of data from portable sequencing could identify the corridor of spread of YFV. These findings reinforce that real-time and continued genomic surveillance strategies can assist in the monitoring and public health responses of arbovirus epidemics.IMPORTANCEArbovirus infections in Brazil including Yellow Fever, Dengue, Zika and Chikungunya result in considerable morbidity and mortality and are pressing public health concerns. However, our understanding of these outbreaks is hampered by limited availability of real time genomic data. In this study, we investigated the genetic diversity and spatial distribution of YFV during the current outbreak in southeastern Brazil. To gain insights into the routes of YFV introduction and dispersion, we tracked the virus by sequencing YFV genomes sampled from non-human primates and infected patients from the southeastern region. Our study provides an understanding of how YFV initiates transmission in new Brazilian regions and illustrates that near-real time genomics in the field can augment traditional approaches to infectious disease surveillance and control.

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Combination of surveillance tools reveals that Yellow Fever virus can remain in the same Atlantic Forest area at least for three transmission seasons

Memórias do Instituto Oswaldo Cruz ◽

10.1590/0074-02760190076 ◽

2019 ◽

Vol 114 ◽

Cited By ~ 9

Author(s):

Filipe Vieira Santos de Abreu ◽

Edson Delatorre ◽

Alexandre Araújo Cunha dos Santos ◽

Anielly Ferreira-de-Brito ◽

Márcia Gonçalves de Castro ◽

...

Keyword(s):

Atlantic Forest ◽

Yellow Fever ◽

Yellow Fever Virus ◽

Forest Area ◽

Fever Virus

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Oviposition behavior of wild yellow fever vector mosquitoes (Diptera: Culicidae) in an Atlantic Forest fragment, Rio de Janeiro state, Brazil

Scientific Reports ◽

10.1038/s41598-021-85752-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shayenne Olsson Freitas Silva ◽

Cecilia Ferreira de Mello ◽

Ronaldo Figueiró ◽

Tatiana Docile ◽

Michele Serdeiro ◽

...

Keyword(s):

Atlantic Forest ◽

Yellow Fever ◽

Rio De Janeiro ◽

Yellow Fever Virus ◽

Diversity Index ◽

Oviposition Behavior ◽

Abiotic Factors ◽

Ground Level ◽

Mosquito Vectors ◽

Janeiro State

AbstractAlthough there are many studies on the control of mosquito vectors of the yellow fever virus (YFV) in tropical forests, there are still few ecological studies regarding abiotic factors effect on these mosquitoes. Here we characterize these effects on oviposition behavior, abundance, and diversity of mosquito vectors of YFV. The study was conducted in Córrego da Luz Municipal Park, in Casimiro de Abreu, Rio de Janeiro state, Brazil, from July 2018 to December 2019. Ovitraps were placed at ground level and 3 m high. The data were tested for normality using the Shapiro–Wilk test, followed by an independent sample analysis, the Mann–Whitney test. The Shannon Diversity Index was used to evaluate the abundance of mosquitos' eggs collected at both ground level and 3 m high. We highlight the presence of Haemagogus janthinomys and Hg. leucocelaenus, primary YFV vectors in forest areas. The abundance of Hg. leucocelaenus (63%), Hg. janthinomys (75%), and Aedes terrens (58%) was higher at the height of 3 m, while Ae. albopictus (52%) was higher at ground level. Aedes albopictus was positively correlated with temperature. Culicidae monitoring is essential for assessing the YFV transmission cycle in Atlantic forest fragments.

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Yellow fever virus outbreak in Brazil under current and future climate

Infectious Disease Modelling ◽

10.1016/j.idm.2021.04.002 ◽

2021 ◽

Vol 6 ◽

pp. 664-677

Author(s):

Tara Sadeghieh ◽

Jan M. Sargeant ◽

Amy L. Greer ◽

Olaf Berke ◽

Guillaume Dueymes ◽

...

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Fever Virus ◽

Future Climate

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Process intensification for the production of yellow fever virus‐like particles (VLPs) as potential recombinant vaccine antigen

Biotechnology and Bioengineering ◽

10.1002/bit.27864 ◽

2021 ◽

Author(s):

Renata G. F. Alvim ◽

Túlio M. Lima ◽

Jerson L. Silva ◽

Guilherme A. P. Oliveira ◽

Leda R. Castilho

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Process Intensification ◽

Recombinant Vaccine ◽

Fever Virus ◽

Vaccine Antigen ◽

Virus Like Particles

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Yellow Fever Outbreak in Eastern Senegal, 2020–2021

Viruses ◽

10.3390/v13081475 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1475

Author(s):

Moussa Moïse Diagne ◽

Marie Henriette Dior Ndione ◽

Alioune Gaye ◽

Mamadou Aliou Barry ◽

Diawo Diallo ◽

...

Keyword(s):

Yellow Fever ◽

Yellow Fever Virus ◽

Mosquito Species ◽

Virus Transmission ◽

Genomic Analysis ◽

Hemorrhagic Fever ◽

Fever Virus ◽

World Health ◽

Effective Vaccine ◽

Ministry Of Health

Yellow fever virus remains a major threat in low resource countries in South America and Africa despite the existence of an effective vaccine. In Senegal and particularly in the eastern part of the country, periodic sylvatic circulation has been demonstrated with varying degrees of impact on populations in perpetual renewal. We report an outbreak that occurred from October 2020 to February 2021 in eastern Senegal, notified and managed through the synergistic effort yellow fever national surveillance implemented by the Senegalese Ministry of Health in collaboration with the World Health Organization, the countrywide 4S network set up by the Ministry of Health, the Institut Pasteur de Dakar, and the surveillance of arboviruses and hemorrhagic fever viruses in human and vector populations implemented since mid 2020 in eastern Senegal. Virological analyses highlighted the implication of sylvatic mosquito species in virus transmission. Genomic analysis showed a close relationship between the circulating strain in eastern Senegal, 2020, and another one from the West African lineage previously detected and sequenced two years ago from an unvaccinated Dutch traveler who visited the Gambia and Senegal before developing signs after returning to Europe. Moreover, genome analysis identified a 6-nucleotide deletion in the variable domain of the 3′UTR with potential impact on the biology of the viral strain that merits further investigations. Integrated surveillance of yellow fever virus but also of other arboviruses of public health interest is crucial in an ecosystem such as eastern Senegal.

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