scholarly journals Pseudoparticle Neutralization Assay for Detecting Ebola- Neutralizing Antibodies in Biosafety Level 2 Settings

2015 ◽  
Vol 61 (6) ◽  
pp. 885-886 ◽  
Author(s):  
Alex W H Chin ◽  
Ranawaka A P M Perera ◽  
Yi Guan ◽  
Peter Halfmann ◽  
Yoshihiro Kawaoka ◽  
...  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adrian Creanga ◽  
Rebecca A. Gillespie ◽  
Brian E. Fisher ◽  
Sarah F. Andrews ◽  
Julia Lederhofer ◽  
...  

AbstractBroadly neutralizing antibodies (bnAbs) have been developed as potential countermeasures for seasonal and pandemic influenza. Deep characterization of these bnAbs and polyclonal sera provides pivotal understanding for influenza immunity and informs effective vaccine design. However, conventional virus neutralization assays require high-containment laboratories and are difficult to standardize and roboticize. Here, we build a panel of engineered influenza viruses carrying a reporter gene to replace an essential viral gene, and develop an assay using the panel for in-depth profiling of neutralizing antibodies. Replication of these viruses is restricted to cells expressing the missing viral gene, allowing it to be manipulated in a biosafety level 2 environment. We generate the neutralization profile of 24 bnAbs using a 55-virus panel encompassing the near-complete diversity of human H1N1 and H3N2, as well as pandemic subtype viruses. Our system offers in-depth profiling of influenza immunity, including the antibodies against the hemagglutinin stem, a major target of universal influenza vaccines.


2020 ◽  
Vol 18 (3) ◽  
pp. 232-236 ◽  
Author(s):  
Kaijin Wang ◽  
Xuetong Zhu ◽  
Jiancheng Xu

1997 ◽  
Vol 2 (4) ◽  
pp. 43-46 ◽  
Author(s):  
Robert J. Emery ◽  
Pek Lee ◽  
James Garman

Heightened interest in pathogens with the potential for aerosol transmission and for which prevention and medical treatment is not readily available has resulted in a need for more work environments that meet Biosafety Level 3 (BSL 3) criteria. Recognizing that the facility-based criteria for BSL 3 cannot be achieved by some existing laboratories, the Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH) biological safety guidelines provide an option for attaining BSL 3 status through the use of Biosafety Level 2 (BSL 2) facilities and strict adherence to BSL 3 practices (BSL 2/3). Inherent to this provision is a greater emphasis on safe work practices. Since the extent to which this approach is actually used in practice is not known, a nationwide mail survey of medical academic and research institutions was conducted to provide an objective indication of the proportion of BSL 3 operations actually being carried out in the BSL 2/3 mode. The results obtained indicate that 2% of activities designated as BSL 3 in the study population actually achieve this level of protection using the BSL 2/3 approach. The findings quantitatively estimate for the first time the proportion of BSL 3 activities being carried out in this fashion, and can serve as a reference point for future studies to evaluate usage trends. The results also demonstrate the utility of flexible, performance-based health and safety guidelines, as a significant amount of clinical and research work is being accommodated with the BSL 2/3 provision.


2016 ◽  
Vol 13 (4) ◽  
pp. 254-264 ◽  
Author(s):  
James D. Johnston ◽  
Ray M. Merrill ◽  
Grant C. Zimmerman ◽  
Scott C. Collingwood ◽  
James C. Reading

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Aleksandra Checinska Sielaff ◽  
Nitin K. Singh ◽  
Jonathan E. Allen ◽  
James Thissen ◽  
Crystal Jaing ◽  
...  

The draft genome sequences of 20 biosafety level 2 (BSL-2) opportunistic pathogens isolated from the environmental surfaces of the International Space Station (ISS) were presented. These genomic sequences will help in understanding the influence of microgravity on the pathogenicity and virulence of these strains when compared with Earth strains.


2013 ◽  
Vol 18 (3) ◽  
pp. 116-121 ◽  
Author(s):  
James D. Johnston ◽  
Steven M. Thygerson ◽  
Michele J. Johnson ◽  
James C. Reading

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