scholarly journals Pseudoparticle Neutralization Assay for Detecting Ebola- Neutralizing Antibodies in Biosafety Level 2 Settings

2015 ◽  
Vol 61 (6) ◽  
pp. 885-886 ◽  
Author(s):  
Alex W H Chin ◽  
Ranawaka A P M Perera ◽  
Yi Guan ◽  
Peter Halfmann ◽  
Yoshihiro Kawaoka ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Neutralization Assay ◽  
Level 2 ◽  
Biosafety Level ◽  
Biosafety Level 2

scholarly journals A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adrian Creanga ◽  
Rebecca A. Gillespie ◽  
Brian E. Fisher ◽  
Sarah F. Andrews ◽  
Julia Lederhofer ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Depth Profiling ◽  
Influenza Viruses ◽  
Effective Vaccine ◽  
Viral Gene ◽  
Broadly Neutralizing Antibodies ◽  
Level 2 ◽  
Biosafety Level ◽  
Major Target

AbstractBroadly neutralizing antibodies (bnAbs) have been developed as potential countermeasures for seasonal and pandemic influenza. Deep characterization of these bnAbs and polyclonal sera provides pivotal understanding for influenza immunity and informs effective vaccine design. However, conventional virus neutralization assays require high-containment laboratories and are difficult to standardize and roboticize. Here, we build a panel of engineered influenza viruses carrying a reporter gene to replace an essential viral gene, and develop an assay using the panel for in-depth profiling of neutralizing antibodies. Replication of these viruses is restricted to cells expressing the missing viral gene, allowing it to be manipulated in a biosafety level 2 environment. We generate the neutralization profile of 24 bnAbs using a 55-virus panel encompassing the near-complete diversity of human H1N1 and H3N2, as well as pandemic subtype viruses. Our system offers in-depth profiling of influenza immunity, including the antibodies against the hemagglutinin stem, a major target of universal influenza vaccines.

Laboratory Biosafety Considerations of SARS-CoV-2 at Biosafety Level 2

2020 ◽  
Vol 18 (3) ◽  
pp. 232-236 ◽  
Author(s):  
Kaijin Wang ◽  
Xuetong Zhu ◽  
Jiancheng Xu
Keyword(s):  
Level 2 ◽  
Biosafety Level ◽  
Biosafety Level 2

Achieving Biosafety Level 3 through the Use of Biosafety Level 2 Facilities and Biosafety Level 3 Practices: A Prevalence Survey of Medical Research and Academic Institutions

1997 ◽  
Vol 2 (4) ◽  
pp. 43-46 ◽  
Author(s):  
Robert J. Emery ◽  
Pek Lee ◽  
James Garman
Keyword(s):  
Research Work ◽  
Health And Safety ◽  
Mail Survey ◽  
Prevalence Survey ◽  
Strict Adherence ◽  
Level 3 ◽  
Level 2 ◽  
Safety Guidelines ◽  
Biosafety Level ◽  
Biosafety Level 2

Heightened interest in pathogens with the potential for aerosol transmission and for which prevention and medical treatment is not readily available has resulted in a need for more work environments that meet Biosafety Level 3 (BSL 3) criteria. Recognizing that the facility-based criteria for BSL 3 cannot be achieved by some existing laboratories, the Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH) biological safety guidelines provide an option for attaining BSL 3 status through the use of Biosafety Level 2 (BSL 2) facilities and strict adherence to BSL 3 practices (BSL 2/3). Inherent to this provision is a greater emphasis on safe work practices. Since the extent to which this approach is actually used in practice is not known, a nationwide mail survey of medical academic and research institutions was conducted to provide an objective indication of the proportion of BSL 3 operations actually being carried out in the BSL 2/3 mode. The results obtained indicate that 2% of activities designated as BSL 3 in the study population actually achieve this level of protection using the BSL 2/3 approach. The findings quantitatively estimate for the first time the proportion of BSL 3 activities being carried out in this fashion, and can serve as a reference point for future studies to evaluate usage trends. The results also demonstrate the utility of flexible, performance-based health and safety guidelines, as a significant amount of clinical and research work is being accommodated with the BSL 2/3 provision.

Factors associated with biosafety level-2 research workers' laboratory exit handwashing behaviors and glove removal compliance

2016 ◽  
Vol 13 (4) ◽  
pp. 254-264 ◽  
Author(s):  
James D. Johnston ◽  
Ray M. Merrill ◽  
Grant C. Zimmerman ◽  
Scott C. Collingwood ◽  
James C. Reading
Keyword(s):  
Factors Associated ◽  
Level 2 ◽  
Biosafety Level ◽  
Biosafety Level 2

scholarly journals Draft Genome Sequences of Biosafety Level 2 Opportunistic Pathogens Isolated from the Environmental Surfaces of the International Space Station

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Aleksandra Checinska Sielaff ◽  
Nitin K. Singh ◽  
Jonathan E. Allen ◽  
James Thissen ◽  
Crystal Jaing ◽  
...  
Keyword(s):  
International Space Station ◽  
Draft Genome ◽  
Space Station ◽  
Opportunistic Pathogens ◽  
Genome Sequences ◽  
International Space ◽  
Environmental Surfaces ◽  
Level 2 ◽  
Biosafety Level ◽  
Biosafety Level 2

The draft genome sequences of 20 biosafety level 2 (BSL-2) opportunistic pathogens isolated from the environmental surfaces of the International Space Station (ISS) were presented. These genomic sequences will help in understanding the influence of microgravity on the pathogenicity and virulence of these strains when compared with Earth strains.

Hand Washing Quality among Biosafety Level 2 Research Laboratory Workers

2013 ◽  
Vol 18 (3) ◽  
pp. 116-121 ◽  
Author(s):  
James D. Johnston ◽  
Steven M. Thygerson ◽  
Michele J. Johnson ◽  
James C. Reading
Keyword(s):  
Research Laboratory ◽  
Hand Washing ◽  
Laboratory Workers ◽  
Level 2 ◽  
Biosafety Level ◽  
Biosafety Level 2
Sign in / Sign up

Export Citation Format

Share Document