Polymerase Chain Reaction for Diagnosing Pneumocystis Pneumonia in Non-HIV Immunocompromised Patients With Pulmonary Infiltrates

CHEST Journal ◽  
2009 ◽  
Vol 135 (3) ◽  
pp. 655-661 ◽  
Author(s):  
Élie Azoulay ◽  
Anne Bergeron ◽  
Sylvie Chevret ◽  
Nicolas Bele ◽  
Benoît Schlemmer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Pneumonia ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Pulmonary Infiltrates ◽  
Polymerase Chain

Diagnosis of pneumocystis pneumonia in a 2-year-old King Charles Cavalier Spaniel using the polymerase chain reaction

2018 ◽  
Vol 47 (1) ◽  
pp. 146-149 ◽  
Author(s):  
Ayeley A. K. Okine ◽  
Seth Chapman ◽  
Roger A. Hostutler ◽  
Robert Livingston
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Pneumonia ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Longest reported case of symptomatic COVID-19 reporting positive for over 230 days in an immunocompromised patient in the United States

2021 ◽  
Vol 9 ◽  
pp. 2050313X2110400
Author(s):  
Bilal Chaudhry ◽  
Lidiya Didenko ◽  
Maaria Chaudhry ◽  
Andrew Malek ◽  
Kirill Alekseyev
Keyword(s):  
Risk Factors ◽  
Polymerase Chain Reaction ◽  
Immunocompromised Patient ◽  
The United States ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Viral Shedding ◽  
Time Period ◽  
Increased Risk ◽  
Polymerase Chain

Coronavirus 2019 (COVID-19) pneumonia was first noted in Wuhan, China. Since the start of the pandemic, there have been millions of cases diagnosed. The average time from onset of symptoms to testing negative SARS-CoV-2 via reverse transcription polymerase chain reaction is roughly 25 days. In patients who continually test positive for COVID-19, it is essential to determine precisely which risk factors contribute to the increase in viral shedding duration. We present a case about a 62-year-old man who has persistently tested positive for COVID-19 for more than 230 days. We followed his treatment course, in which he had been hospitalized multiple times since the onset of symptoms back in April 2020. We have determined that patients with immunosuppression, especially those taking corticosteroids, are at increased risk of prolonged viral shedding. It is essential to continually monitor these immunocompromised patients as they required a greater time period in order to have an appropriate immune response in which antibodies are created.

Low Sensitivity of a Nested Polymerase Chain Reaction in Oropharyngeal Washings for the Diagnosis of Pneumocystis Pneumonia in HIV-Infected Patients

CHEST Journal ◽  
2005 ◽  
Vol 128 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Kennedy Nyamande ◽  
Umesh G. Lalloo ◽  
Dennis York ◽  
Mogambal Naidoo ◽  
Elvis M. Irusen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Pneumonia ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Low Sensitivity

scholarly journals A new single run polymerase chain reaction assay for cyclosporiasis in immunocompromised patients

2021 ◽  
Vol 7 (3) ◽  
pp. 207-212
Author(s):  
Rakesh Singh ◽  
Manish Katiyar ◽  
Reena Gulati ◽  
Sreejith Parameswaran ◽  
Abdoul Hamide ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Tertiary Care ◽  
Cost Effective ◽  
People Living With Hiv ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Cross Sectional ◽  
Stool Samples ◽  
Polymerase Chain

causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen in people living with HIV/AIDS (PLHA), post-renal transplant (PRT) patients and immunocompromised children (IC). Diagnostic microscopy for the oocysts of the parasite is less sensitive, requiring examination of multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay for the detection of and it was used to know the hospital based prevalence of cyclosporiasis. A cross-sectional study was conducted from June 2016 to October 2020 in a tertiary care teaching hospital. A new single run amplification PCR-based diagnostic assay was developed for . Stool samples were collected from 121 PLHA, 135 PRT and 79 immunocompromised children (IC) other than PLHA and PRT. All stool samples were examined for the presence of oocysts as well as tested with new PCR assay. Modified Ziehl-Neelsen staining of the concentrated stool smear did not reveal oocysts of species in any stool specimen. However, new PCR assay detected in 2 stool specimens – one from a PLHA patient and another from a PRT patient, giving a prevalence of 0.6% (2/335), 0.8% (1/121) in PLHA and 0.7% (1/135) in PRT. It was not detected in IC. Cyclosporiasis is infrequent in southern part of India. The new single run PCR assay developed by us is simple and cost effective molecular assay for the detection of .

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