Production and Secretion Patterns of Cloned Glucoamylase in Plasmid-Harboring and Chromosome-Integrated Recombinant Yeasts Employing an SUC2 Promoter

2000 ◽  
Vol 87 (2) ◽  
pp. 81-94 ◽  
Author(s):  
Hyung Joon Cha ◽  
Hee Jeong Chae ◽  
Suk Soon Choi ◽  
Young Je Yoo
Keyword(s):  
Suc2 Promoter ◽  
Recombinant Yeasts

scholarly journals Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant Saccharomyces cerevisiae

2010 ◽  
Vol 2 (2) ◽  
pp. 351-361
Author(s):  
A. A. Saleh ◽  
S. Hamdan ◽  
N. Annaluru ◽  
S. Watanabe ◽  
M. R. Rahman ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzyme Activity ◽  
Protein Engineering ◽  
Agricultural Waste ◽  
Xylitol Dehydrogenase ◽  
Ethanol Conversion ◽  
Waste Biomass ◽  
Wild Type ◽  
Coenzyme Specificity ◽  
Recombinant Yeasts

Agricultural waste biomass has already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR) and the protein engineered xylitol dehydrogenase (XDH) (with NADP) of Pichia stipitis.  These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomyces cerevisiae as judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARS strain, in which NADP+-dependent XDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.  Keywords: Agricultural waste biomass; Protein engineering; Xylitol dehydrogenase; Xylose-fermentation; Eethanol production. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i2.2882               J. Sci. Res. 2 (2), 351-361 (2010) 

Analysis of SUC2 Promoter Structure by Nucleosome Scanning

2011 ◽  
pp. 321-333 ◽  
Author(s):  
Jennifer Chang ◽  
Ales Vancura
Keyword(s):  
Promoter Structure ◽  
Suc2 Promoter

Fuel Ethanol Production from Lignocellulosic Raw Materials Using Recombinant Yeasts

2010 ◽  
pp. 261-291 ◽  
Author(s):  
Grant Stanley ◽  
Brbel Hahn-Hgerdal
Keyword(s):  
Ethanol Production ◽  
Raw Materials ◽  
Fuel Ethanol ◽  
Recombinant Yeasts

Effects of GAL10-SUC2 promoter combinations on SUC2 gene expression in S. cerevisiae

1993 ◽  
Vol 13 (2) ◽  
pp. 77-83
Author(s):  
Feng Bo ◽  
Li Yu-yang ◽  
Chen Zhao-cong
Keyword(s):  
Gene Expression ◽  
Suc2 Promoter ◽  
Suc2 Gene

Preparation of high-activity whole cell biocatalysts by permeabilization of recombinant yeasts with alcohol

2000 ◽  
Vol 89 (6) ◽  
pp. 554-558 ◽  
Author(s):  
Yan Liu ◽  
Yasuya Fujita ◽  
Akihiko Kondo ◽  
Hideki Fukuda
Keyword(s):  
High Activity ◽  
Whole Cell ◽  
Recombinant Yeasts

scholarly journals A new class of histone H2A mutations in Saccharomyces cerevisiae causes specific transcriptional defects in vivo.

1995 ◽  
Vol 15 (4) ◽  
pp. 1999-2009 ◽  
Author(s):  
J N Hirschhorn ◽  
A L Bortvin ◽  
S L Ricupero-Hovasse ◽  
F Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Activation Function ◽  
Histone H2a ◽  
Yeast Saccharomyces Cerevisiae ◽  
New Class ◽  
Suc2 Promoter ◽  
Encoding Gene ◽  
Transcription Activation Function

Nucleosomes have been shown to repress transcription both in vitro and in vivo. However, the mechanisms by which this repression is overcome are only beginning to be understood. Recent evidence suggests that in the yeast Saccharomyces cerevisiae, many transcriptional activators require the SNF/SWI complex to overcome chromatin-mediated repression. We have identified a new class of mutations in the histone H2A-encoding gene HTA1 that causes transcriptional defects at the SNF/SWI-dependent gene SUC2. Some of the mutations are semidominant, and most of the predicted amino acid changes are in or near the N- and C-terminal regions of histone H2A. A deletion that removes the N-terminal tail of histone H2A also caused a decrease in SUC2 transcription. Strains carrying these histone mutations also exhibited defects in activation by LexA-GAL4, a SNF/SWI-dependent activator. However, these H2A mutants are phenotypically distinct from snf/swi mutants. First, not all SNF/SWI-dependent genes showed transcriptional defects in these histone mutants. Second, a suppressor of snf/swi mutations, spt6, did not suppress these histone mutations. Finally, unlike in snf/swi mutants, chromatin structure at the SUC2 promoter in these H2A mutants was in an active conformation. Thus, these H2A mutations seem to interfere with a transcription activation function downstream or independent of the SNF/SWI activity. Therefore, they may identify an additional step that is required to overcome repression by chromatin.

scholarly journals New Approach of Highly Efficient Fermentation Process for Bioethanol using Xylose as Agriculture Residues

2017 ◽  
Vol 26 (1) ◽  
pp. 1-12
Author(s):  
Abu Saleh Ahmed ◽  
Seiya Watanabe ◽  
Sinin Hamdan ◽  
Tsutomu Kodaki ◽  
Keisuke Makino
Keyword(s):  
Enzyme Activity ◽  
Agricultural Waste ◽  
Xylose Reductase ◽  
Pichia Stipitis ◽  
Ethanol Conversion ◽  
Waste Biomass ◽  
Wild Type ◽  
Coenzyme Specificity ◽  
Recombinant Yeasts

Agricultural waste biomass has already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still exist to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR) and the protein engineered xylitol dehydrogenase (XDH) (with NADP) of Pichia stipitis.  These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomyces cerevisiae as judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARS strain, in which NADP+-dependent XDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild–type XDH.      

scholarly journals New Approach of Highly Efficient Fermentation Process for Bio ethanol using Xylose as Agricultur e Residues

2017 ◽  
Vol 26 (1) ◽  
Author(s):  
Abu Saleh Ahmed ◽  
Seiya Watanabe ◽  
Sinin Hamdan ◽  
Tsutomu Kodaki ◽  
Keisuke Makino
Keyword(s):  
Enzyme Activity ◽  
Agricultural Waste ◽  
Xylose Reductase ◽  
Ethanol Conversion ◽  
Wild Type ◽  
Coenzyme Specificity ◽  
Genes Encoding ◽  
Efficient Fermentation ◽  
Recombinant Yeasts

Agricultural waste biomasshas already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still exist to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR)and the protein engineered xylitol dehydrogenase (XDH)(with NADP) of Pichiastipitis. These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomycescerevisiaeas judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARSstrain, in which NADP+-dependentXDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild–type XDH. 

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