Growth performance and plumage development of young turkeys fed on diets with graded levels of sulfur-containing amino acids

2015 ◽  
Author(s):  
Magdalena Kubińska ◽  
Emilia Mróz ◽  
J. Jankowski ◽  
Z. Zduńczyk
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Plumage Development ◽  
Sulfur Containing

Methionine-Specific Staining of Collagen

1982 ◽  
Vol 40 ◽  
pp. 294-295
Author(s):  
E.M. Kuhn ◽  
K.D. Marenus ◽  
M. Beer
Keyword(s):  
Amino Acids ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Electron Microscopic ◽  
Good Correspondence ◽  
Specific Staining ◽  
Type Iii ◽  
Different Types ◽  
Sulfur Containing

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

scholarly journals PSIV-6 Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acids Transporters in Nursery Pigs Fed with Low Protein Diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 281-282
Author(s):  
Cedrick N Shili ◽  
Mohammad Habibi ◽  
Julia Sutton ◽  
Jessie Barnes ◽  
Jacob Burchkonda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Growth Performance ◽  
Mrna Abundance ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Blood Calcium ◽  
Low Protein ◽  
Protein Diets ◽  
Standard Protein

Abstract Moderately low protein (MLP) diets can help decrease nutrient excretion from the swine production. However, MLP diets negatively impact growth performance. We hypothesized that supplementing MLP diets with phytogenics may reduce the negative effects of these diets on growth. The objective of this study was to investigate the effect of a phytogenic water additive (PWA; Herbanimal®) on growth performance, blood metabolite and gene expression of amino acids transporters in pigs fed with MLP diets. Forty-eight weaned barrows were allotted to six dietary treatments (n = 8) for 4 weeks: >CON-NS: standard protein diet-no PWA; CON-LS: standard protein diet-low PWA dose (4 ml/L); CON-HS: standard protein diet-high PWA dose (8 ml/L); LP-NS: low protein diet-no PWA; LP-LS: low protein diet-low PWA dose (4 ml/L); LP-HS: low protein diet- high PWA dose (8 ml/L). Feed intake and body weight were recorded daily and weekly, respectively. At week 4, blood and tissue samples were collected and analyzed for metabolites using a chemistry analyzer and amino acid transporters using qPCR, respectively. The data were analyzed by univariate GLM (SPSS®) and the means were separated using paired Student’s t-test corrected by Benjamini-Hochberg. Pigs fed CON-HS improved the average daily gain and serum calcium and phosphorus concentrations compared to CON-NS. Pigs fed LP-LS had higher serum phosphorus and blood urea nitrogen compared to the pigs fed with LP-NS. The mRNA abundance of SLC7A11 in the jejunum was lower in CON-LS and CON-HS compared to CON-NS. Additionally, mRNA abundance of SLC6A19 in the jejunum of pigs fed with LP-LS was higher compared to LP-NS and lower in CON-HS relative to pigs fed with CON-LS. In conclusion, PWA improved the growth performance of pigs fed standard protein diets but not low protein diets. Further, the PWA improved the concentrations of blood calcium and phosphorous in pigs fed MLP diets. Funding: Agrivida and Animal Health and Production and Animal Products: Improved Nutritional Performance, Growth, and Lactation of Animals from the USDA-NIFA.

scholarly journals Growth performance and accretion of selected amino acids in response to three levels of dietary lysine fed to fast- and slow-growing broilers

2021 ◽  
Vol 100 (4) ◽  
pp. 100998
Author(s):  
D.H. Tran ◽  
J. Th. Schonewille ◽  
C. Pukkung ◽  
S. Khempaka
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Slow Growing

scholarly journals 129 Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on growth performance, nutrient digestibility, and colonic pH in diets fed to weanling pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 101-102
Author(s):  
Jaron R Lewton ◽  
Adrienne Woodward ◽  
Ronny Moser ◽  
Kyan M Thelen ◽  
Adam J Moeser ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Distal Colon ◽  
Nutrient Digestibility ◽  
Nursery Pigs ◽  
Fiber Digestion ◽  
Males And Females ◽  
Dietary Treatments ◽  
The Impact

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.

scholarly journals 138 Effects of Increasing Soybean Meal in Corn-based Diets on Growth Performance of Late-finishing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 70-71
Author(s):  
Julia P Holen ◽  
Robert D Goodband ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Joel M DeRouchey
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Soybean Meal ◽  
Fixed Effects ◽  
Average Length ◽  
Average Daily Gain ◽  
Random Effect ◽  
Finishing Pigs ◽  
Distillers Grains With Solubles ◽  
Dietary Treatments

Abstract Two experiments were conducted to determine the effects of increasing levels of soybean meal (SBM) replacing feed grade amino acids in corn or corn-dried distillers grains with solubles (DDGS)-based diets on growth performance of late finishing pigs. In both experiments, there were 22 to 27 pigs per pen and 14 pens per treatment. Average length of the experiments was 35 (Exp. 1) and 29 days (Exp. 2). Diets were balanced to contain 0.70% SID Lys and 2,667 or 2,610 kcal NE/kg for Exp. 1 and 2, respectively. Minimum amino acid ratios relative to Lys were: Ile, 55; Met&Cys, 60; Thr, 65; Trp, 19.5, and Val, 70. Dietary crude protein ranged from 10.1 to 15.2 for Exp. 1 and 13.6 to 19.4 for Exp. 2. The statistical model considered fixed effects of treatment, linear and quadratic contrasts, and random effect of block. In Exp.1, 1,793 pigs (L337×1050, PIC; initially 104.9 ± 1.4 kg) were fed corn-based diets and pens of pigs were assigned to 1 of 5 dietary treatments with increasing SBM from 5 to 20%. Overall, average daily gain (ADG) and gain-to-feed (G:F) increased (linear; P < 0.05) as SBM increased with the greatest improvement observed as SBM increased from 5 to 8.75%, with little improvement thereafter. In Exp. 2, 1,827 pigs (L337×1050, PIC; initially 97.9 ± 1.1 kg) were used in a similar study as Exp. 1, but all diets contained 25% DDGS and SBM levels increased from 0 to 16%. Overall, G:F and final bodyweight of pigs marginally improved (linear and quadratic, respectively; P < 0.10) as SBM increased, with the greatest performance observed when diets contained 8% SBM. These results suggest that increasing SBM up to 8% at the expense of feed grade amino acids in corn or corn-DDGS-based diets improved ADG or G:F in late-finishing pigs.

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