scholarly journals Laccase and Manganese Peroxidase (MnP) Activities in the White-Rot Fungus Trametes hirsuta in Response to Aromatic Compounds

2020 ◽  
Vol 23 (2) ◽  
pp. 66
Author(s):  
Isa Nuryana ◽  
Zidny Ilmiah ◽  
Ade Andriani ◽  
Yopi Yopi
Keyword(s):  
Gallic Acid ◽  
Caffeic Acid ◽  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Veratryl Alcohol ◽  
Syringic Acid ◽  
White Rot ◽  
Trametes Hirsuta

The current interest in exploring white-rot fungi has been concentrated in increasing their ligninolityc enzyme production such as laccase and manganese peroxidase (MnP) due to the great value in industrial application. The presence of appropiate inducers can enhance the enzyme production. Hence, the aim of the study was to investigate the activity of  laccase and MnP in response to various aromatic compounds. The fungus Trametes hirsuta was cultured in Glucose Yeast Peptone (GYP) broth (pH 5.0) with the addition of 0.5% of sorghum biomass as substrate. The cultures were then incubated on rotary shaker at 150 rpm at 27oC. The 7-day-old cultures were then supplemented with different aromatic compounds, namely caffeic acid, gallic acid, syringic acid, trans-cinnamic acid, vanillin, and veratryl alcohol. Our results demonstrated that veratryl alcohol, gallic acid and vanillin gave an inductive effect on the value of laccase and MnP activities, with vanillin showing the highest induction. On the contrary, the level of laccase and MnP activities remained low in the presence of aromatic compound such as syringic acid, trans-cinnamic acid and caffeic acid. To conclude, our study reveals that aromatic compounds can be potential as inducers and may contribute to the improvement of laccase and MnP productivity by T. hirsuta.

Effect of aromatic compounds on growth and ligninolytic enzyme production of two white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus

1998 ◽  
Vol 44 (9) ◽  
pp. 872-885 ◽  
Author(s):  
Anand Sethuraman ◽  
Danny E Akin ◽  
Jason G Eisele ◽  
Karl-Erik L Eriksson
Keyword(s):  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Hyphal Growth ◽  
White Rot ◽  
Ceriporiopsis Subvermispora ◽  
Cyathus Stercoreus ◽  
Benzaldehyde Derivatives

Seven benzoic acid, ten cinnamic acid, and five benzaldehyde derivatives were tested for their effects on hyphal growth and production of laccase and manganese peroxidase by Ceriporiopsis subvermispora FP 90031-sp and Cyathus stercoreus ATCC 36910. Derivatives tested included phenolic compounds and their corresponding unsubstituted and O-methylated derivatives. Benzaldehyde derivatives were more toxic to both fungi than the corresponding benzoic and cinnamic acid derivatives. Hyphal growth was generally increased at a low concentration of 1 mM, while higher concentrations of 5-10 mM mostly resulted in less or no growth. Hyphal growth and enzyme production response were compound specific. However, generally monomethoxylated compounds were more toxic than compounds with an additional methoxyl group. Cyathus stercoreus was more sensitive than Ceriporiopsis subvermispora to most of the compounds tested and thus showed poorer growth. Cyathus stercoreus produced higher concentrations of manganese peroxidase than Ceriporiopsis subvermispora for all the compounds tested, whereas laccase activity was higher in Ceriporiopsis subvermispora for most of the compounds tested. Di- and tri-methoxylated compounds induced more laccase and manganese peroxidase activities than the corresponding hydroxylated derivatives. At 1 mM levels, 3,4-dimethoxycinnamic acid induced the greatest increase in laccase production for Ceriporiopsis subvermispora and Cyathus stercoreus (245 and 290% of control, respectively). Syringic acid induced manganese peroxidase (MnP) to 536% of that in control for Ceriporiopsis subvermispora, and both 3,4-dimethoxycinnamic acid and 3,4,5-trimethoxycinnamic acid induced MnP to over 300% of control for Cyathus stercoreus. The results provide a body of information on the effects of specific aromatic compounds on two potentially industrially important fungi. Key words: biomass conversion, aromatic compounds, white rot fungi, fungal growth, enzyme production.

Effect of growth conditions on the production of manganese peroxidase by three strains of Bjerkandera adusta

2001 ◽  
Vol 47 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Yuxin Wang ◽  
Rafael Vazquez-Duhalt ◽  
Michael A Pickard
Keyword(s):  
Rice Bran ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Scale Up ◽  
Growth Conditions ◽  
White Rot ◽  
Stirred Tank ◽  
Bjerkandera Adusta ◽  
Stirred Tank Reactors ◽  
Cereal Bran

We were looking for a strain of Bjerkandera adusta that produces high titres of manganese peroxidase under optimal conditions for large-scale enzyme purification. We have chosen two strains from the University of Alberta Microfungus Collection and Herbarium, UAMH 7308 and 8258, and compared the effects of growth conditions and medium composition on enzyme production with the well-characterized strain BOS55 (ATCC 90940). Of four types of cereal bran examined, rice bran at 3% (w/v) in 60 mM phosphate buffer pH 6 supported the highest levels of enzyme production. Using 100 mL medium in 500-mL Erlenmeyer flasks, maximum enzyme levels in the culture supernatant occurred after about 10 days of growth; 5.5 U·mL–1 for UAMH 7308, 4.4 U·mL–1 for UAMH 8258, and 1.7 U·mL–1 for BOS55, where units are expressed as micromoles of Mn-malonate formed per minute. Growth as submerged cultures in 10-L stirred tank reactors produced 3.5 U·mL–1 of manganese peroxidase (MnP) by UAMH 8258 and 2.5 U·mL–1 of MnP by 7308, while enzyme production by BOS55 was not successful in stirred tank reactors but could be scaled up in 2-L shake flasks containing 400 mL rice bran or glucose – malt – yeast extract (GMY) – Mn-glycolate medium to produce MnP levels of 1.7 U·mL–1. These results show that the two strains of B. adusta, UAMH 7308 and 8258, can produce between two and three times the manganese peroxidase level of B. adusta BOS55, that they are good candidates for scale up of enzyme production, and that the rice bran medium supports higher levels of enzyme production than most previously described media.Key words: growth conditions, cereal bran, manganese peroxidase, Bjerkandera adusta, white rot fungi.

scholarly journals The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME

2021 ◽  
Vol 13 (2) ◽  
pp. 243-249
Author(s):  
Yohanes Bernard Subowo ◽  
Arwan Sugiharto
Keyword(s):  
Palm Oil ◽  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
Laccase Activity ◽  
Ligninolytic Enzymes ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Growth Media ◽  
Palm Oil Mill ◽  
Low Activity

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we  obtained new data regarding the activity of laccase and its ability to degrade POME. 

scholarly journals High-Performance Thin-Layer Chromatography Densitometric Method for the Simultaneous Determination of Three Phenolic Acids in Syzygium aromaticum (L.) Merr. & Perry

2008 ◽  
Vol 91 (5) ◽  
pp. 1169-1173 ◽  
Author(s):  
Subha Rastogi ◽  
Madan M Pandey ◽  
Ajay K S Rawat
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Simultaneous Determination ◽  
Caffeic Acid ◽  
Correlation Coefficient ◽  
Phenolic Acids ◽  
High Performance ◽  
Syringic Acid ◽  
Syzygium Aromaticum

Abstract A simple, precise, and rapid high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous determination of 3 phenolic acids, i.e., gallic acid, caffeic acid, and syringic acid, in the dried buds of Syzygium aromaticum, commonly known as clove. HPTLC was performed on silica gel 60F254 plates with tolueneethyl acetateformic acid (8 2 1) mobile phase and densitometric scanning at 280 nm. The method was validated for selectivity, linearity, precision, and repeatability. Instrumental precision coefficient of variation (CV) was 0.88, 0.93, and 0.98% and repeatability of the method (CV) was 0.76, 0.64, and 0.69% for gallic acid, caffeic acid, and syringic acid, respectively. The linear concentration ranges were 4003200 ng/spot with a correlation coefficient of 0.993 for gallic acid, 4403520 ng/spot with a correlation coefficient of 0.994 for caffeic acid, and 4004000 ng/spot with a correlation coefficient of 0.993 for syringic acid. The average recoveries of gallic acid, caffeic acid, and syringic acid were 96.3, 95.7, and 92.4%, respectively. Gallic acid, caffeic acid, and syringic acid were present at levels of 1.58, 0.06, and 0.05% (w/w), respectively, in S. aromaticum. This method is simple, accurate, precise, and economical and can be used for routine quality control.

scholarly journals Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

1997 ◽  
Vol 75 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Tamara Vares ◽  
Annele Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Growth Conditions ◽  
Fungal Species ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Air Atmosphere ◽  
Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

scholarly journals Biodecolorization of Remazol Brilliant Blue–R dye by Tropical White-Rot Fungi and Their Enzymes in The Presence of Guaiacol

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Sita Heris Anita ◽  
Fitria Ningsih ◽  
Dede Heri Yuli Yanto
Keyword(s):  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Static Condition ◽  
White Rot ◽  
Brilliant Blue ◽  
Synthetic Dyes ◽  
Trametes Hirsuta ◽  
Remazol Brilliant Blue R ◽  
Dyes Decolorization

The ability of the tropical white-rot fungi and their enzyme to decolorize synthetic dyes was investigated. Production of lignin-modifying enzymes (LMEs) from the three new isolated fungi, namely Trametes hirsuta D7, Ceriporia sp. BIOM 3, and Cymatoderma dendriticum WM01 were observed for 9 days incubation under static condition. The results showed that the LMEs production enhanced in the present of guaiacol. T. hirsuta D7 produced only laccase (Lac), with the highest activity was 22.6 U/L on the 5th-day of the cultivation. At the same time, Ceriporia sp. BIOM 3 and C. dendriticum WM01 secreted both laccases (Lac) with the activities 0.2 U/L and 1.0 U/L, respectively, and manganese peroxidase (MnP) with the activities 0.1 U/L and 1.0 U/L, respectively. Among the fungi, T. hirsuta D7 efficiently degraded 65% Remazol Brilliant Blue–R (RBBR) dye within 72 h using the only laccase. This study shows that laccase may have a major role in synthetic dyes' decolorization process, followed by MnP and LiP.

scholarly journals Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33

3 Biotech ◽  
2019 ◽  
Vol 9 (11) ◽  
Author(s):  
Pisit Thamvithayakorn ◽  
Cherdchai Phosri ◽  
Nipon Pisutpaisal ◽  
Sukhumaporn Krajangsang ◽  
Anthony J. S. Whalley ◽  
...  
Keyword(s):  
Oil Palm ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot ◽  
White Rot Fungus ◽  
Decanter Cake ◽  
Peroxidase Enzyme

scholarly journals HPLC Determination of Polyphenols from Calendula officinalis L. Flowers

2017 ◽  
Vol 21 (2) ◽  
pp. 97-101 ◽  
Author(s):  
Adina Frum
Keyword(s):  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Cinnamic Acid ◽  
Chemical Compounds ◽  
Hplc Method ◽  
Calendula Officinalis ◽  
Calendula Officinalis L

AbstractRomanian spontaneous flora provides a lot of resources for the determination of different chemical compounds. This study uses flower samples fromCalendula officinalisL. extracted through maceration. The chemical compounds determined were: (+)-catechin, caffeic acid, chlorogenic acid, cinnamic acid, ferulic acid, gallic acid, rutin, resveratrol and quercetin. They were analyzed by using an optimized HPLC method. (+)-Catechin, caffeic acid, chlorogenic acid and quercetin could not be identified in the analyzed samples. The greatest amount of phenolic compound found was rutin and the smallest quantity was determined for ferulic acid. The quantified compounds have proven to have benefits regarding human health, thus they can be used as functional compounds and can be included in food products and food supplements.

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