scholarly journals Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice

2021 ◽  
Vol 23 (2) ◽  
pp. 55-62
Author(s):  
Lia Meilawati ◽  
Teni Ernawati ◽  
Rizna Triana Dewi ◽  
Megawati Megawati ◽  
Sukirno Sukirno
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Water Solvent ◽  
Activity Test ◽  
Gallic Acid Equivalent

AbstractExtraction of ripened noni juice has been carried out using ethanol and water. The purpose of this study was to determine the total phenolic, total flavonoids, scopoletin, content and antioxidant activity of the ripened noni juice extract. The activity test was carried out on the ripened noni juice extract without and with the addition of ethanol and water solvent. The phytochemical assays of ethanol extract of the ripened noni juice showed that thetotal phenolic content was 3,94 mg gallic acid equivalent/g extract, the total flavonoids was 0,59 mg quercetin equivalent /g extract, the IC50 antioxidant activity was 24,92 mg/L, scopoletin content was 2,45 mg/gram.Keywords : Antioxidant, flavonoids, phenolic, ripened noni juice, scopoletin

scholarly journals Bekul Fruit, Potential Pharmafood from Northern Region of Bali Island, Indonesia: Selected Phytochemical Analyses and Antioxidant Activity of Its Ethanol Extract.

2021 ◽  
Vol 14 (1) ◽  
pp. 477-483
Author(s):  
Agung Nova Mahendra ◽  
I Nyoman Tri Pramartha
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Activity Index ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Northern Region ◽  
Total Phenolic ◽  
Tannin Content ◽  
Dpph Assay ◽  
Gallic Acid Equivalent

Background: Bekulfruit from Northern region (Buleleng regency), Bali, Indonesia, is commonly consumed fresh in the island of Bali or processed as local delicacy and used as part of religious offerings. Up to date, there is no data regarding the taxonomy, phytochemical composition, and antioxidant properties of this Balinese fruit.This study was aimed to investigate total phenolic content, tannin content and antioxidant activity of the ethanol extract of bekul fruit obtained from Banjar district, Buleleng regency, Bali. Methods: Total phenolic compound was quantified in terms of gallic acid equivalent (GAE) by using Folin-Ciocalteu method, mean while tannin content was determined in terms of tannic acid equivalent (TAE). IC50of the extract was determined using DPPH assay, and subsequently used in the calculation of antioxidant activity index (AAI) using the formula of Scherer and Godoy (2009). Results: Bekul plant was revealed as Ziziphusjujuba Mill. Total phenolic and tannin content of the extract was 29.48 mg/100 g GAE and 91.06 mg/100 g TAE, respectively. Thevalue of IC50was 77.40 mg/ml, with antioxidant activity index (AAI) of 50.94. Conclusion:Ethanol extract of bekul (Ziziphusjujuba Mill.) fruit contains phenolic and tannin compounds. This extract is found to scavenge free radicals and possess very strong antioxidant activityin vitro. Taken together, these findings lead to the notion that bekul fruit from Northern region of Bali, Indonesia, is a promising pharma food

scholarly journals Uji Aktivitas Antioksidan dari Ekstak Etanol Daun Nipah (Nypa fruticans Wurmb.) dengan Metode DPPH

2018 ◽  
Vol 1 (2) ◽  
pp. 205-212
Author(s):  
Riza Silvia Anggraini ◽  
Sudarmi Sudarmi ◽  
Herawaty Ginting
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Colorimetric Method ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Nypa Fruticans ◽  
Gallic Acid Equivalent

Tumbuhan Nipah (Nypa fruticans Wurmb.) merupakan tumbuhan suku Arecaceae yang tumbuh di lingkungan hutan yang bermanfaatsebagai obat sakit perut, diabetes dan obat penurun panas . Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan serta total fenol dan total flavonoid dari Ekstrak Etanol Daun Nipah (Nypa fruticans Wurmb.). Penelitian ini meliputi penyiapan sampel, pemeriksaan karakteristik simplisia, pembuatan ekstrak dengan metode maserasi, dan pengujian aktivitas antioksidan dari ekstrak etanol daun nipah  dengan metode perangkapan radikal bebas menggunakan DPPH (1,1- difenil-2-pikrilhidrazil), pada panjang gelombang 515,50 nm,  dibandingkan  dengan vitamin C sebagai blanko positif, serta pengujian nilai kandungan total fenol ekstrak etanol daun nipah setara asam galat (Gallic Acid Equivalent (GAE)) dengan metode kolorimetri menggunakan reagen Folin-Ciocalteu pada panjang gelombang 775 nm dan pengukuran nilai kandungan total flavonoid ekstrak etanol daun nipah setara kuersetin (Quercetin Equivalent (QE)) dengan metode kolorimetri menggunkan aluminium klorida pada panjang gelombang 436,50 nm dengan menggunakan spektrofotometri UV-Visibel. Dari penelitian yang dilakukan diperoleh hasil uji aktivitas antioksidan yang menunjukkan bahwa ekstrak etanol daun nipah mempunyai aktivitas antioksidan yang sangat kuat dengan nilai IC50 sebesar 9,662 ppm serta nilai kandungan total fenol sebesar 85,51 mg/g GAE ekstrak dan nilai kandungan total flavonoid sebesar 12,49 mg/g QE ekstrak. Nipah plant (Nypa fruticans Wurmb.) Is a plant of the Arecaceae family that grows in a forest environment that is useful as a medicine for stomach pain, diabetes and febrifuge. This study aims to determine antioxidant activity, total phenol and total flavonoids from the Ethanol Extract of Nipah Leaves (N. fruticans). This study included the preparation of samples, checking the characteristics of simplicia, making extracts by maceration method, and testing the antioxidant activity of ethanol extract of Nipah leaves by using free radical scavenging method using DPPH (1,1-diphenyl-2-pikrilhidrazil), at a wavelength of 515.50 nm, compared with vitamin C as a positive blank, as well as testing the value of the total phenol content of ethanol extract of gallic leaf equivalent of gallic acid (Gallic Acid Equivalent (GAE)) using the colorimetric method using Folin-Ciocalteu reagent at a wavelength of 775 nm and measuring the value of total flavonoid content ethanol extract of Nipah leaves equivalent to quercetin (Quercetin Equivalent (QE)) by colorimetric method using aluminum chloride at a wavelength of 436.50 nm using UV-Spectrophotometry. Antioxidant activity test showed that the Nipah leaf ethanol extract had a very strong antioxidant activity with an IC50 value of 9.662 ppm and a total phenol content of 85.51 mg / g GAE extract and a total flavonoid content of 12, 49 mg / g QE extract.

scholarly journals PHYTOCHEMICAL PROFILING, ASSESSMENT OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF ETHNOMEDICINAL PLANT, MEYNA SPINOSA FROM ASSAM

2019 ◽  
pp. 61-63
Author(s):  
REKHA BORA ◽  
SEEMA KHAKHALARY ◽  
TAPAN DUTTA
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Radical Scavenging Assay

Objective: The present study qualitative phytoconstituents examine the total phenol, total flavonoid content (TFC), and antioxidant efficiencies traditionally used plant, Meyna spinosa. Methods: Chemical profiling, estimation of total phenolic content (TPC), TFC, and antioxidant activity of ethanol extracts of M. spinosa have performed by applying standard protocols. Antioxidant activity of leaf and stem was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. TPC and TFC of the plant were assessed using Folin–Ciocalteu colorimetric and aluminum colorimetric assay, respectively. Results: The findings of the study exhibit that ethanol extract of M. spinosa is proved to be the presence of phytoconstituents (7/9) such as alkaloids, terpenoids, saponins, tannins, phytosterols, flavonoids, and phenolic compounds, while carbohydrate, fixed oils, and fats are unavailable. In addition, phenolic compositions of ethanol extract of leaf and stem; 93.21±2.93 and 54.33±0.69 mg gallic acid equivalents/g extract, respectively; TFCs of leaf and stem have recorded as 61.55±1.21 and 37.55±1.28 mg quercetin equivalents/g extract, respectively. Antioxidant efficiency of both leaf and stem is tested using DPPH radical scavenging assay as IC50 20.68±0.32 and 50.99±0.56 μg/ml, respectively. Conclusion: From the above results, it has concluded that the ethanol extract of the M. spinosa leaves and stems seizes rich phytoconstituents which can be applied in food technology, drug industries, ethnopharmacological fields, etc., for the development of healthiness and to battle against negative health consequences.

scholarly journals Uji Fitokimia dan Antioksidan dari Daun Yantan (Blumea chinensis DC)

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 32
Author(s):  
Griffin A. D. Rambi ◽  
Vanda S. Kamu ◽  
Max R. J. Runtuwene
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Activity Test

Telah dilakukan penelitian untuk menentukan kandungan fitokimia dan aktifitas antioksidan dari ekstrak etanol daun yantan (Blumea chinensis DC). Kandungan fitokimia yang diuji adalah total fenolik dan total flavonoid. Total fenolik menggunakan metode Folin-Ciocalteu  (Yadaf dan Agarwala, 2010), lalu untuk mengetahui total flavonoid menggunakan aluminium klorida (Sultana et al., 2011), dan pengujian aktifitas antioksidan menggunakan 1,1-difenil-2-pikrilhidrazil (DPPH) (Subedi et al., 2011). Hasil penelitian menunjukan kandungan fenolik total sebesar 26,75 mg/L dan kandungan flavonoidnya sebesar 12,56 mg/L,untuk pengujian aktifitas antioksidan didapatkan nilai IC50 sebesar 240,19 mg/L.Research have been done to determining the phytochemical compound and antioxidant activity of yantan (Blumea chinensis DC) leaves. The phytochemical compound which tested is phenolic total and flavonoid total. Phenolic total using Folin-Ciocalteu  method (Yadaf dan Agarwala, 2010), then to determining the total flavonoid using aluminium chloride (Sultana et al., 2011), and test of the antioxidant activity using 1,1-difenil-2-pikrilhidrazil DPPH (Subedi et al., 2011).The result of the total phenolic content was showed 26,75 mg/L and the total flavonoid content is 12,56 mg/L, for antioxidant activity test obtained the value IC50 is 240,19 mg/L.

scholarly journals Antibacterial and antioxidant activities of ethanol extract of Artocarpus lacucha Buch-Ham. leaves against Propionibacterium acne

2021 ◽  
Vol 332 ◽  
pp. 08007
Author(s):  
Panal Sitorus ◽  
Mahatir Muhammad ◽  
Henni Cintya ◽  
and Denni Satria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Degenerative Diseases ◽  
Inhibitor Concentration ◽  
Propionibacterium Acne

Antioxidants can neutralize free radicals that can affect biological systems in the body that can cause degenerative diseases. Antibacterial active molecules have been identified from plants, and these chemicals are being utilized to develop new and promising treatments. The Mobe plant (Artocarpus lacucha Buch-Ham.) is a species of Artocarpus member of the Moraceae family. Includes a high proportion of phenolic compounds (flavonoids and phenolic acid). This research obtained to know about the biological activity of Artocarpus lacucha Buch-Ham and their total flavonoid and total phenolic content. The extract was prepared using the soxhletation technique using ethanol as the solvent. Antibacterial activity was determined using minimum inhibitor concentration. Propionibacterium acne can be inhibited at concentrations ranging from 300 ppm to 25 ppm. The DPPH technique was used to assess antioxidant activity. The samples’ total flavonoid and phenolic content were measured colorimetrically. The DPPH test revealed an antioxidant activity of 23.33 g/mL, which was used to compute the IC50. The extract’s total phenolic (117.44 mg GAE/g) and flavonoid (3.54 mg QE/g) content were determined to be high.

scholarly journals Phytochemical Screening of Leaves and Roots of Stylochiton Borumensis: A Medicinal Plant

2019 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Gallic Acid ◽  
Tannic Acid ◽  
Plant Extracts ◽  
Phenolic Content ◽  
Colorimetric Assay ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Total Flavonoids Content ◽  
Gallic Acid Equivalent

The aim of this study was to find out phytochemical classes present in various extracts of Stylochiton borumensis and to determine the total phenolic, flavonoids and tannins content in different plant extracts. Total phenols, flavonoids and tannins content was determined by folin-ciocalteu assay, aluminum chloride colorimetric assay and ferric chloride colorimetric assay respectively. Different extracts of S. Borumensis showed the presence of alkaloids, sterols/ triterpenoid, flavonoids, tannins and coumarins. The phenolic, flavonoids and tannins contents of plant extracts using gallic acid (Y= 0.0007x + 0.1078, r² = 0.9997), quercetin (Y= 0.0007x + 0.053, r²= 0.9984) and tannic acid (Y= 0.0009x + 0.043) as standards. The total phenolic content ranged from 460 to 530 mg/g gallic acid equivalent in leaves and from 240 to 520 mg/1 gallic acid equivalent in roots. The total flavonoids content was ranged from 140.870 to 360.750 mg/1 quercetin equivalent in leaves and from 138.678 to 357.670 mg/1 quercetin equivalent in roots. The total tannins contents ranged from 210 to 300 mg/l tannic acid equivalent in leaves and 190 to 270 mg/1 tannic acid equivalent in roots. The study showed significant amount of gallic acid, quercetin and tannic acid equivalents were present in S.Borumensis extracts which may responsible for valuable pharmacological property of the plant.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF SILYBUM MARIANUM CULTURES GROWN ON DIFFERENT GROWTH REGULATORS

2017 ◽  
Vol 9 (4) ◽  
pp. 44 ◽  
Author(s):  
Hattem Mekky ◽  
Samah El Sohafy ◽  
Rasha Abu El-khair ◽  
Amr El Hawiet
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Growth Regulators ◽  
Phenolic Content ◽  
Wild Plant ◽  
Total Phenolic ◽  
Silybum Marianum ◽  
Polyphenolic Content ◽  
Trolox Equivalent ◽  
Total Polyphenolic Content

Objective: The present study was designed to study the effect of supplementing Silybum marianum cultures with different combinations and concentrations of growth regulators on the total phenolic content and the antioxidant activity of the produced cultures.Methods: The total polyphenolic content of 23 extracts of calli was calculated as µg of gallic acid/mg of dry weight extract (DWE) using Folin–Ciocalteu reagent. The antioxidant activity was calculated as trolox equivalent antioxidant capacity using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay for extracts containing more than 10 µg gallic acid/mg DWE.Results: Most of the combinations showed an increase in the total polyphenolic concentration compared to the wild plant. However, cultures grown on media supplemented with combinations of benzyl aminopurine (BAP) and gebberellic acid (Gb), adenine (Ad) and kinetin (Kin), BAP and indole acetic acid (IAA) possessed the highest total polyphenolic contents 20.5, 13.09 and 12.15 µg gallic acid/mg DWE, respectively. However, only (BAP+Gb), (BAP+1-Naphthaleneacetic acid (NAA), IAA, kin and (BAP+Ad) showed a significant increase in the antioxidant activity calculated as trolox equivalent with 2.65, 1.56, 1.56, 1.35 and 1.22 folds increase over the wild plant, respectively.Conclusion: The results obtained clearly indicated that changing the growth regulator system of Silybum marianum cultures serves as an easy and reliable method for increasing the total phenolic content and the antioxidant activity of cultures.

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