scholarly journals Phytochemical Screening of Leaves and Roots of Stylochiton Borumensis: A Medicinal Plant

2019 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Gallic Acid ◽  
Tannic Acid ◽  
Plant Extracts ◽  
Phenolic Content ◽  
Colorimetric Assay ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Total Flavonoids Content ◽  
Gallic Acid Equivalent

The aim of this study was to find out phytochemical classes present in various extracts of Stylochiton borumensis and to determine the total phenolic, flavonoids and tannins content in different plant extracts. Total phenols, flavonoids and tannins content was determined by folin-ciocalteu assay, aluminum chloride colorimetric assay and ferric chloride colorimetric assay respectively. Different extracts of S. Borumensis showed the presence of alkaloids, sterols/ triterpenoid, flavonoids, tannins and coumarins. The phenolic, flavonoids and tannins contents of plant extracts using gallic acid (Y= 0.0007x + 0.1078, r² = 0.9997), quercetin (Y= 0.0007x + 0.053, r²= 0.9984) and tannic acid (Y= 0.0009x + 0.043) as standards. The total phenolic content ranged from 460 to 530 mg/g gallic acid equivalent in leaves and from 240 to 520 mg/1 gallic acid equivalent in roots. The total flavonoids content was ranged from 140.870 to 360.750 mg/1 quercetin equivalent in leaves and from 138.678 to 357.670 mg/1 quercetin equivalent in roots. The total tannins contents ranged from 210 to 300 mg/l tannic acid equivalent in leaves and 190 to 270 mg/1 tannic acid equivalent in roots. The study showed significant amount of gallic acid, quercetin and tannic acid equivalents were present in S.Borumensis extracts which may responsible for valuable pharmacological property of the plant.

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

scholarly journals Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice

2021 ◽  
Vol 23 (2) ◽  
pp. 55-62
Author(s):  
Lia Meilawati ◽  
Teni Ernawati ◽  
Rizna Triana Dewi ◽  
Megawati Megawati ◽  
Sukirno Sukirno
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Water Solvent ◽  
Activity Test ◽  
Gallic Acid Equivalent

AbstractExtraction of ripened noni juice has been carried out using ethanol and water. The purpose of this study was to determine the total phenolic, total flavonoids, scopoletin, content and antioxidant activity of the ripened noni juice extract. The activity test was carried out on the ripened noni juice extract without and with the addition of ethanol and water solvent. The phytochemical assays of ethanol extract of the ripened noni juice showed that thetotal phenolic content was 3,94 mg gallic acid equivalent/g extract, the total flavonoids was 0,59 mg quercetin equivalent /g extract, the IC50 antioxidant activity was 24,92 mg/L, scopoletin content was 2,45 mg/gram.Keywords : Antioxidant, flavonoids, phenolic, ripened noni juice, scopoletin

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

scholarly journals DETERMINATION OF TOTAL PHENOLIC CONTENT FROM PLANT EXTRACTS USED IN COSMETIC PURPOSE

2021 ◽  
Vol 21 (1) ◽  
pp. 247-260
Author(s):  
SIMONA DOBRINAS ◽  
ALINA SOCEANU
Keyword(s):  
Measurement Uncertainty ◽  
Gallic Acid ◽  
Acid Concentration ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Limit Of Detection ◽  
Total Phenolic ◽  
Spectrometric Method ◽  
Calibration Equation

Spectrometric analysis belongs to a group of measurement methods used for their simplicity and large selectivity to solve various problems of analytes. The total phenolic content of different plant extracts used for cosmetic purpose was determined by a slightly modified version of traditionally Folin-Ciocalteau method. The objective of this work was the validation of UV-Vis spectrometric method, investigation of uncertainty sources when measuring gallic acid concentration and evaluation of the calibration equation effect on measurement uncertainty of UV– Vis spectrophotometer. Validation was performed by studying analytical curve linearity (R2=0.9995) and range (37.5 – 225 mg L-1), estimated limit of detection (LOD, 0.11mg L-1) and limit of quantification (LOQ, 0.37 mg L-1), precision (%RSD, 0.14 – 1.34), recoveries (83-110%) and stability (%RSD, 0.8 – 2.83). To obtain more representative values for precision, recoveries and stability simultaneous replicates at different times, on different matrices including plant (marigold, chamomile and lavender) were performed during the study period. The validated method was successfully applied to determine TPC in marigold extracts. For chamomile and lavender extracts, the spectrometric method presented only acceptable precision, among all the performance parameters studied. The sources of the gallic acid concentration measurement uncertainty include purity, volume of flasks, mass and the calibration equation. The results indicated that the uncertainty components from purity were the smallest. An important source of the uncertainty was the mass.The volumes of the volumetric flasks had only modest effect on the uncertainty. The contribution of calibration equation is the greatest from all sources.

scholarly journals Estimation of total tannins, total phenolics and total flavonoids of different extracts of cedrela toona roxb. Fruits.

2021 ◽  
Vol 10 (1) ◽  
pp. 05-10
Author(s):  
Shah Kinjal H ◽  
Patel Piyush M.
Keyword(s):  
Tannic Acid ◽  
Colorimetric Assay ◽  
Water Extract ◽  
Soxhlet Extraction ◽  
Dry Weight ◽  
Powdered Material ◽  
Aluminium Chloride ◽  
Assay Method ◽  
Total Phenolic ◽  
Total Flavonoids

Air dried powdered material of the fruits of cedrela toona Roxb. was successively extracted with petroleum ether, hexane, acetone, methanol and water extract by soxhlet extraction and subjected to various qualitative chemical tests to determine presence of various phytoconstituents like alkaloids, glycosides, carbohydrates, phenolics and tannins, phtosterols, fixed oils and fats, proteins, amino acids, flavonoids, saponins etc. Total flavonoids content was measured with the ‘Aluminium Chloride Colorimetric Assay Method’. Total Phenolic content in the aqueous and methanol extracts of fruits of Cedrela toona Roxb. were determined using the Folin Denis Method and expressed as mg of tannic acid equivalents per gram dry weight of extract. This study helps researchers for developmentof isolation method of active ingredient having vast pharmacological effects. Keywords: Cedrela toona, total flavonoids, total tannins and phenolics, Tannic acid

scholarly journals Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin

2016 ◽  
Vol 5 (5) ◽  
pp. 528-539
Author(s):  
Saoussen Bouacida ◽  
Hayet Ben Haj koubaier ◽  
Ahmed Snoussi ◽  
Marie Laure Fauconnier ◽  
Nabiha Bouzouita
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Plant Part ◽  
The Other ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Seed Extracts

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) µmol /g DW for seeds, flowers, leaves, roots and stems, respectively. Results showed that seeds are rich in phenolics as they contain highest amounts of phenolics ranging from 27.6±0.5 to 33.47±0.5 mg GAE/g extract as compared to all other parts. Leaves and flowers had a significantly higher total phenolic content than stems and roots in all samples (p < 0.05). According to statistical analysis, the investigated seed extracts with values between (16.20±0.10-18.50±0.10 mg QE/g) exhibited the highest total flavonoids content, followed by leaves (13.00±0.40-15.80±0.30mg QE/g), flowers (10.40±0.40-12.90±0.90 mg QE/g) and stems (7.80±0.20- 9.80±0.70 mg QE/g). Antioxidant activity tested by DPPH, ABTS and FRAP assays, was higher for seeds, leaves and flowers than the other studied organs. These organs were characterized by a significantly high content in glucoerucin, nasturtin and epiprogroitrin, respectively.

scholarly journals ESTIMATION OF PHENOLIC CONTENT, FLAVONOID CONTENT, ANTIOXIDANT, AND ALPHA-AMYLASE INHIBITORY ACTIVITY OF SOME SELECTED PLANTS FROM SIRAHA DISTRICT NEPAL

2020 ◽  
pp. 18-23
Author(s):  
Numer Alam ◽  
Khaga raj Sharma
Keyword(s):  
Plant Extract ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Brine Shrimp ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Tinospora Cordifolia ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content

Objectives: The purpose of this research was to estimate the phenolic content, flavonoid content, antioxidant, antibacterial, and α-amylase inhibitory activity of some selected plants such as Anethum sowa, Trigonella foenum-graecum, Lepidium sativum, Cuscuta reflexa, Eclipta alba, Leucas cephalotes, and Tinospora cordifolia collected from Siraha district of Nepal using in vitro studies. Methods: Methanol extracts of these medicinal plants were prepared by cold percolation method. Preliminary phytochemical screening was performed by color differentiation method. Total phenolic and flavonoid content were estimated by Folin-Ciocalteu reagent method and aluminum chloride colorimetric method. Antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Furthermore, the α-amylase enzyme inhibitory activity was studied using starch as a substrate, pancreatic α-amylase as the enzyme, and acarbose as standard. Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. The phenolic content estimation showed T. foenum-graecum 939.764±0.01, L. sativum 551.63±0.02, A. sowa 306.34±0.06, L. cephalotes 233.19±0.03, T. cordifolia 211.76±0.02, E. alba 202.67±0.02, and C. reflexa Roxb. 145.09±0.09 mg milligram gallic acid equivalent per gram. The flavonoid content estimation showed T. cordifolia 852.07±0.11, L. sativum 553.81±0.05, E. alba 322.13±0.02, A. sowa 329.02±0.05, L. cephalotes 164.93±0.02, and C. reflexa Roxb. 146.37±0.00 mg milligram quercetin equivalent per gram. The antioxidant potential showed by E. alba IC50 33.48±0.82 μg/ml and the values ranged from E. alba 33.48±0.82 to A. sowa 47.62±1.09 μg/ml. The α-amylase inhibitory activity showed by A. sowa 76.78±2.00–E. alba 777.36±9.66 μg/ml. The result of brine shrimp toxicity showed LC50 value >1000 μg/ml. Among the seven plant extracts, only the plant extract of E. alba showed a zone of inhibition 14 mm against Staphylococcus aureus. Conclusions: The plant extract of T. foenum-graecum showed the highest phenolic content, and T. cordifolia showed the highest flavonoid content. The highest antioxidant potential exhibited by E. alba and the highest α-amylase inhibition activity showed by A. sowa. The plant extract of E. alba showed moderate antibacterial activity against S. aureus. All plant extracts were found non-toxic against brine shrimp larvae although further study is needed to assess its mechanism of action.

Studies on the Pholyphenolics Compounds Extraction from Vaccinium Fruits

2008 ◽  
Vol 59 (5) ◽  
Author(s):  
Mariana Pop ◽  
Alfa Xenia Lupea ◽  
Violeta Turcus
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Aluminum Chloride ◽  
Total Phenolics ◽  
Colorimetric Assay ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Bioactive Properties ◽  
Wide Range ◽  
Phenolic And Flavonoid

Bilberries are known for their bioactive properties such as antioxidant activity. They contain a wide range of flavonoids and phenolic acids that show antioxidant activity. In the present work, the total phenolic and flavonoid contents, correlated with antioxidant activity of Vaccinium extracts were determined. The content of total phenolics in the extracts was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). The content of total flavonoids was measured also spectrophotometrically by using the aluminum chloride colorimetric assay. Effects of ethanol and methanol concentrations of the solutions on the contents of flavonoids and phenolic in extracts were investigated.

scholarly journals Phytochemical Analysis and Antimicrobial Activity of Iris kashmiriana and Iris ensata Extracts against Selected Microorganisms

2018 ◽  
Vol 8 (6) ◽  
pp. 28-34
Author(s):  
Javeed Iqbal Wagay ◽  
Kirti Jain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Iris Ensata ◽  
Total Flavonoids Content ◽  
Phytochemical Constituent

Infectious diseases are the second leading cause for worldwide death. Treatment of infections continues to be difficult in modern time because of the severe side effects of some drugs and the growing resistance to antibacterial agents. Over the past few decades the use of antibiotics is under threat as many commonly used antibiotics have become less effective against certain illnesses due to emergence of multi drug-resistant bacteria. In the present study the effects of 3 types of solvents, chloroform, ethyl acetate and methanol were investigated to determine the presence of various phytochemical constituent, total phenolic content, total flavonoids content and in vitro antimicrobial activity from rhizomes of Iris kashmiriana (Kashmir Iris) and Iris ensata (Japanese Iris), belong to family Iridaceae. The reason for selecting in vitro method was to minimize the usage of experimental animals. The antimicrobial activity of chloroform, ethyl acetate and methanol extract of rhizomes of Iris kashmiriana and Iris ensata were evaluated on bacterial strains of Bacillus cereus, Pseudomonas auregenosa, Proteus vulgaris and Eschirichia coli and fungal strains of Candida albicans and Aspergillus niger by agar well diffusion method. The preliminary phytochemical studies and quantitative analysis of alkaloids, phenol and flavonoids were performed by well reported method. These extracts were further subjected to TLC (Thin layer chromatography analysis).  The chemical contents of the Iris kashmiriana and Iris ensata were presented as total phenolic content and total flavonoids content. Phytochemical screening of the extract showed the presence of some common compounds like phenols, terpenoids, flavonoids, carbohydrate etc. The antimicrobial potential of the plant extract was evaluated against different bacterial species which shows significant inhibitory action against all the tested bacterial and fungal strain. Methanolic extract was found to be more active than chloroform and ethyl acetate extracts. It reveals that the methanol soluble components of the plant are highly active against the above mentioned microorganism. Keywords: Iris kashmiriana, Iris ensata, Phytochemical constituent, Total phenolic content, Total flavonoids content, In vitro antimicrobial activity

Total Phenolic and Flavonoid Content and Antibacterial Properties of Polygonatum orientale Desf and Tilia dasystyla

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Parichehr Hanachi ◽  
Roshanak Zarringhalami ◽  
Ertugrul Kaya
Keyword(s):  
Staphylococcus Aureus ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Total Flavonoids ◽  
Flavonoid Content ◽  
Water Solvent ◽  
Total Flavonoids Content ◽  
Phenolic And Flavonoid

Background: Secondary metabolites of plants such as phenol and flavonoids can neutralize free radicals. The antioxidant and cytotoxic activities of plants are related to phenolic or flavonoids compounds. The occurrence of drug resistance to antimicrobial drugs has led to the use of medicinal herbs in the treatment of infections. Antibiotic resistant of Staphylococcus aureus has become a major problem in the treatment of diseases. Objectives: The present study aimed to determine total phenolic content (TPC) and total flavonoids content (TFC) of Polygonatum orientale Desf and Tilia dasystyla and evaluate their antibacterial effects on Staphylococcus aureus bacterium. Total phenolic content of Polygonatum orientale Desf and Tilia dasystyla has not been previously investigated. Methods: Total phenolic and flavonoid content of P. orientale Desf and T. dasystyla extracts were determined using colorimetric methods of Folin-Ciocalteu and aluminum chloride. Antimicrobial activities of the extracts were evaluated by microdilution broth and disc diffusion methods to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Results: The results showed that total flavonoids content of Polygonatum orientale Desf with the value of 7.9 ± 0.040 mg/g DW extracted with diluted water solvent and boiling method and total phenolic contents of Tilia dasystyla with the value of 62.13 ± 0.073 mg/g DW extracted with methanol solvent and boiling method were the highest amount. Methanol extract of Polygonatum orientale Desf had more antibacterial activities with the MBC and MIC values of 0.140 mg/ mL and 8 ± 0.4 mm zone of inhibition. Conclusions: T. dasystyla and P. orientale Desf contain bioactive compounds such as phenolic and flavonoids that can be used as a promising option in pharmacognostical studies for the treatment of S. aureus infections.

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