scholarly journals AMINO-ACID COMPOSITION OF ATRIPLEX PATULA L. AND AMARANTHUS RETROFLEXUS L. (AMARANTHACE-AE) GROWING IN CENTRAL YAKUTIA

2018 ◽  
pp. 69-74
Author(s):  
Иван (Ivan) Васильевич (Vasil'evich) Воронов (Voronov)

The research goal is comparative study the amino acid composition of seeds and leaves of Atriplex patula L. and Amaranthus retroflexus L. from the flora of Central Yakutia (the Republic of Sakha (Yakutia). Leaves and seeds of A. patula and A. retroflexus were sampled in August 2016 in the fruiting phase. Biochemical and amino acid composition, the content of crude protein, fat, calcium and phosphorus of the abovementioned plants was studied at the premises of the State budgetary institution of the Republic of Sakha (Yakutia) "Yakut Republican Veterinary Testing Laboratory". From 14 studied amino acids, 9 are stated to be irreplaceable. The total amount of the studied amino acids in A. patula made up 3.3±0.2% in leaves and 3.6±0.2% in seeds; while A. retroflexus contained 4.2±0.2% in leaves and 3.8±0.2% in seeds. The irreplaceable amino acid composition included lysine, leucine and isoleucine, methionine, valine, threonine, arginine, histidine, and phenylalanine. The interchangeable amino acid composition was represented by tyrosine, proline, serine, alanine and glycine. The sum of irreplaceable amino acids in A. patula made up 2.07±0.10% in leaves and 2.30±0.12% in seeds; in A. retroflexus the irreplaceable amino acids totaled 2.63±0.13% in leaves and 2.20±0.11% in seeds. It should be noted that histidine is absent in seeds and the low content of phenylalanine in seeds – 2.8 times and leaves – 4.5 times in A. patula compared to A. retroflexus. The obtained data indicate that in the leaves and seeds of A. retroflexus in comparison with A. patula, the content of crude protein is 1.5 times higher, the calcium content is higher to 2.3 times, the phosphorus content is lower: in leaves 3.3 times, in seeds - in 1.4 times. The results of the study show the biological value and perspectivity of the two studied species as a promising source of natural biological active substances to be used in medicine and agriculture.

1993 ◽  
Vol 57 (03) ◽  
pp. 500-502
Author(s):  
R. H. King ◽  
C. J. Rayner ◽  
M. Kerr

Effects of dietary protein concentration on the amino acid composition of sow's milk was studied using 10 crossbred first-litter sows. At parturition, diets containing similar concentrations of digestible energy but with either 63 or 238 g crude protein per kg and 4.4 and 15.1 g lysine per kg respectively, were given through lactation (five sows per treatment). The ratios of other amino acids to lysine were in excess of those currently recommended for lactating sows. Dietary protein level significantly affected milk yield, gave higher milk protein output (P> 0.05) but was without significant effect on the proportions of amino acids in the milk.


2013 ◽  
Vol 38 (2) ◽  
pp. 103-113 ◽  
Author(s):  
Józef Bąkowski ◽  
Ryszard Kosson

The study of the nutritional value and amino acid composition of the mushroom (<i>Agaricus bisporus</i>) was carried out with the Somycel 653 strain cultivated on a synthetic compost containing rye straw, broiler chicken manure and gypsum. In 4 developmental stages of the whole fruit-bodies and the caps and stipes the following were determined: dry matter, vitamin C, nitrates, total nitrogen, crude protein (N × 4.38) and amino acid composition. It was observed that in all stages of development the levels of total N and crude protein are significantly higher in the caps than in the stipes. From the amino acid composition it appears that the caps of stage 4 contain the highest amounts of essential and total amino acids as compared with caps, stipes and whole sporophores found in any developmental stage. This is correlated with the opening of the mushrooms in stage 4. It was observed that the nitrates level tends, to be higher in the cap than in the stipe.


2021 ◽  
pp. 113-119
Author(s):  
Evgeny Evgenievich Kurdyukov ◽  
Elena Fedorovna Semenova ◽  
Ol'ga Aleksandrovna Vodopyanova ◽  
Yakov Petrovich Moiseev ◽  
Olesya Petrovna Rodina ◽  
...  

Dried stevia leaves (Stevia rebaudiana Bertoni) were used as objects of research. We studied the leaves of stevia varieties Ramon sweetener grown in the Penza region, the leaves of stevia varieties Ramon sweetener grown in the Tver region, Krasnodar region, the Republic of Crimea, as well as imported raw stevia from India and Paraguay. The purpose of this work is a comparative study of the amino acid composition of raw stevia grown in different conditions. The amino acid composition of stevia leaves (Stevia rebaudiana Bertoni) was revealed by capillary electrophoresis.13 amino acids were identified, of which eight are "essential" (lysine, phenylalanine, histidine, leucine, isoleucine, methionine, valine, threonine). The proportion of essential amino acids in stevia raw materials ranged from 2.99 to 4.64%. The content of interchangeable acids was: tyrosine from 0.24% to 0.36%, Proline from 0.44 to 0.68%, serine from 0.77 to 1.03%, alanine from 0.48 to 0.83%, glycine from 0.40 to 0.68%. The total amount of amino acids detected is higher in the Ramon sweetener variety grown in the Penza region (9.52%) compared to other samples, the lowest amount is found in stevia grown in Paraguay (6.46%). The results obtained indicate the prospects for further studies of the amino acid composition of Stevia rebaudiana and can characterize this species as a source of valuable medicinal substances with a wide range of pharmacological activity.


2019 ◽  
pp. 217-223
Author(s):  
Ekaterina Yur'yevna Bakova ◽  
Yuriy Vladimirovich Plugatar ◽  
Nadezhda Nikolayevna Bakova ◽  
Dmitriy Alekseyevich Konovalov

The homeland of Myrthus communis L. is Mediterranean region, in the countries of which its wild-growing and cultivated varieties grow. In the collection of the Nikitsky Botanical Gardens – National Scientific Center of the Russian Academy of Sciences the economic-valuable and morphological features of nine cultivars of this species have been studied. The plant is considered as a promising industrial medicinal culture. Contamination of medicinal plants with heavy metals and other toxic elements is an existing problem, which is associated with serious concern of international organizations on the quality and safety of medicinal raw materials. It is now established that trace constituents, entering into connection with chemical regulators of metabolism, including amino acids, participate in various biochemical processes, stimulate and normalize metabolism. The objective of this work is to study the elemental and amino acid composition of leaves of Myrthus communis L., cultivated in the Nikitsky Botanical Gardens. The study of Myrthus communis L. ultimate composition has been done with due regard to General Monograph requirements "The identification of heavy metals and arsenium in medicinal raw materials and plant preparations" for State Pharmacopeia of XIII edition. The research of the amino acid profile has been done subject to State All-Union Standard 32195-2013 (ISO 13903:2005). As a result of research it has been established that leaves of Myrthus communis L. accumulate 28 elements and 16 amino acids. The content of heavy metals and arsenium in the studied samples did not exceed the maximum permissible values for medicinal plant raw materials established by the Russian State Pharmacopoeia. The total content of amino acids in the leaves of myrtle was 11.65%. Among the amino acids found, glutamic and aspartic acids prevailed. The least in the leaves was methionine. The obtained results will be used for follow up standardization of myrtle raw materials as a promising source of medicinal plant raw material.


2019 ◽  
Vol 49 (5) ◽  
pp. 94-97
Author(s):  
В. М. Ludu

The results of the comparative study of yak blood parameters by amino-acid composition depending on the season of the year are presented. The research was conducted in the Republic of Tuva. The object of research were adult female yaks after the fi rst or more calving. The studied animals were at the pasture in the highlands all year round. The material of the research was whole blood stabilized by heparin and yak serum. Blood was taken in spring and autumn from the jugular vein. The determination of blood biochemical parameters and the biometric processing of the results were carried out by generally accepted methods. 14 amino acids were revealed. Of these, seven are non-essential amino acids (asparagine, serine, glutamine, glycine, alanine, histidine, arginine) and seven are essential (threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine). The amino-acid content in the blood of yaks living in highlands under low partial pressure did not vary signifi cantly during the study period. The content of non-essential and essential amino acids was recorded in the ratio of 50 : 50, regardless of the season of the year. In autumn, compared to spring, serine content increased 2.43 times, asparagine –1.05 times, valine – 1.07, leucine – 1.07 times. An increase in phenylalanine in the autumn period may indicate its suffi cient content in grass eaten by yaks. Features of the interior indicators of yaks, common in the Republic of Tuva, are the result of their long year-round pasture maintenance in extreme climatic conditions of the highlands.


2020 ◽  
pp. 215-223
Author(s):  
Arsen Shamsudinovich Ramazanov ◽  
Shamsiyat Abdulmedzhidovna Balaeva

The object of the study was partially skimmed fruits (meal) of milk Thistle [Silybum marianum (L.) Gaertn.], growing on the territory of the Republic of Dagestan. The aim of the study was to determine the protein content in the meal of milk Thistle fruits and its amino acid composition; assessment of the biological value of the studied protein. It was found that partially skimmed fruits of milk Thistle contain 21.72% protein, which is about 2 times more than the seeds of grain crops. The composition of milk Thistle protein by ion exchange and liquid chromatography identified and quantified 18 amino acids, including all essential amino acids, which account for 30.11% of the protein. In the composition of the protein in greater quantities than in the protein standard found essential amino acids: phenylalanine+tyrosine, threonine, isoleucine and valine. Arginine, glutamic acid, aspartic acid, glycine, and serine have been found to be the most abundant of the amino acids in milk Thistle protein. It is established that the protein of milk Thistle fruits is comparable to the protein of winter wheat and rye varieties by formal indicators of biological value, and significantly exceeds the protein of sunflower meal.


1955 ◽  
Vol 8 (4) ◽  
pp. 537 ◽  
Author(s):  
DH Simmonds

The amino acid composition of 16-hr 6N HCI hydrolysates of three qualities of commercially classified wools has now been determined using the technique of Moore and Stein (1951). In this paper the results obtained on samples of Merino 70's and Corriedale 56's wool are compared with those previously reported for Merino wool of 64's quality. The overall pattern of the amino acid composition of the three wools is similar although small variations between the wools are observed with some of the amino acids.


1973 ◽  
Vol 134 (2) ◽  
pp. 431-436 ◽  
Author(s):  
W. Ferdinand ◽  
W. Bartley ◽  
V. Broomhead

Amino acid analyses of mitochondrial membranes are compared with the amino acid composition of whole mitochondria (Alberti, 1964) and found to be very similar except in the cystine content. The composition of the endogenous amino acids found in freshly prepared mitochondria has been established and shown to differ considerably from the amino acid composition of membranes or whole mitochondria. The amino acids produced during anaerobic incubation of mitochondria at pH7.4, on the other hand, resemble the membrane in composition, supporting the view that neutral proteinase activity is responsible for their appearance. Aerobic incubation produces a similar pattern of amino acids except that amino acids such as proline, serine, asparagine, glutamic acid and glutamine, which can be metabolically utilized under aerobic conditions, are present to a smaller extent. The presence of large relative concentrations of endogenous taurine, cysteic acid and oxidized glutathione and the accumulation of taurine during incubation is found. The selective retention of taurine and cysteic acid within the mitochondria is established. It is proposed that the first step in the degeneration of isolated mitochondria results from lipid hydroperoxide accumulation caused by the lack of glutathione reductase in isolated mitochondria.


1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.


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