scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF PLANT AGENTS

2020 ◽  
pp. 187-193
Author(s):  
Yekaterina Vladimirovna Ferubko ◽  
Valeriy Nikolayevich Zelenkov ◽  
Anatoliy Andreyevich Lapin ◽  
Tamara Darizhapovna Dargayeva
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Lipid Analysis ◽  
Free Radical Oxidation ◽  
Inhibitory Effect ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
White Rats ◽  
Total Antioxidant

The aim of the research is to determine the total antioxidant activity of in vitro collection of antihepatotoxic action consisting of roots and rhizomes of Inula helenium L., grass Centaurium erythraea Rafn., flowers Tanacetum vulgare L., fruits Rosa sp., fruits of Crataegus sp. аnd its separate components by the method of colonometric titration and determination of antioxidant activity of dry extract (conditional name "Pentafite") obtained from this collection under conditions of experimental tetrachloride of carbon hepatitis in white rats. A method of chemiluminescent lipid analysis was used to evaluate the free radical oxidation of lipids in liver in test animals. It has been found that collection of antihepatotoxic action and plants included in it have antioxidant activity in vitro, at the same time synergistic effect on manifestation of total antioxidant activity of collection is revealed. Pharmacotherapy of experimental hepatitis in experimental rats by administration of Pentafite at a dose of 300 mg/kg has been found to significantly reduce the chemiluminometric values of lipids, which characterize the intensity of free radical reactions. "Pentafite" has a pronounced inhibitory effect on hyperlipoperoxidation in animal liver in case of toxic damage to it. The established antioxidant activity of collecting antihepatotoxic action and Pentafite extract may be important for their use in the treatment and prevention of hepatobiliary diseases.

scholarly journals Critical conditions as a logical and appropriate chain of iron methabolism disorders (the summary of experimental studies)

2013 ◽  
Vol 59 (6) ◽  
pp. 700-709 ◽  
Author(s):  
Y.P. Orlov ◽  
V.N. Lukach ◽  
V.T. Dolgih ◽  
E.L. Soboleva ◽  
A.V. Ivanov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Rheological Properties ◽  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Critical Conditions ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Total Antioxidant

It is revealed that in all models of critical conditions will activate the free-radical oxidation, decreasing the total antioxidant activity, the concentration of transferrin decreases in the serum of the blood, increase of the concentration of ferritin, the rheological properties of the blood are violated and the signs of endothelial dysfunction are identified. Pre-entered deferoxamine in the dose of 80 mg/kg reduced the intensity of free-radical oxidation processes, restoring the antioxidant potential, concentration of the transferrin, and a lower level of ferritin, contributed to the normalization of blood rheological properties and a reduction of the extent endothelium destruction as a result of the reduction Fe2+ concentration in blood serum.

scholarly journals In Vitro Inhibitory Effect on Carbohydrates Digestion and Antioxidant Potential of Garlic Scape (P06-076-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Dammah Otieno ◽  
Salma Altuwaijri ◽  
Hye Won Kang
Keyword(s):  
Antioxidant Activity ◽  
Digestive Enzymes ◽  
Radical Scavenging Activity ◽  
Health Effect ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Abstract Objectives Inhibition of carbohydrate absorption from intestine is an efficient way to prevent type 2 diabetes. Some edible plants have shown the potential as anti-diabetic agents by inhibiting carbohydrate-digestive enzymes in intestine. In terms of edible plant's health effect, bioactive compounds’ stability during the process of digestive enzymes has been commonly questioned. Therefore, the objectives of this study were to 1) examine inhibitory effect of garlic scape (GS), the green flower head of garlic on carbohydrate-degrading enzymes and 2) investigate antioxidant activity and total phenolic content (TPC) of GS treated with intestinal digestive enzymes. Methods GS extract (GSE) was prepared with 80% methanol (v/v) by homogenization, sonication, and filtration, and evaporation. α-glucosidase, maltase, glucoamylase, sucrase, α-amylase inhibitory activities were determined by measuring glucose hydrolyzed from nitrophenyl-glucopyranoside, maltose, starch, sucrose, and starch, respectively. To prepare gastrointestinal enzyme-digested GSE, GSE was digested with pepsin and a pancreatin-bile solution. GSE and gastrointestinal enzyme-digested GSE were assessed for TPC using a Folin-Ciocalteu reagent and for total antioxidant and radical scavenging activities by the following methods, phosphomolybdeum, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods, respectively. Results GSE (20 mg/ml) showed 30%, 50%, and 42% inhibition on α-glucosidase, maltase, and glucoamylase enzyme activities, respectively. GSE (50 mg/ml) showed 55.1% DPPH radical and 5782 mg vitamin C equivalent (VCE)/g ABTS radical scavenging activities. Total antioxidant activity of the GSE (1.5 mg/ml) exhibited 2.76 mg VCE/g. Consistent with its antioxidant property, the GSE showed a dose-dependent increase in TPC. Gastrointestinal enzymatic treatment resulted in approximately 46% reduction in DPPH and ABTS radical scavenging activity, total antioxidant activity, and TPC of the GSE. Conclusions Garlic scape has the potential as a natural anti-diabetic agent. Garlic scape keeps beneficial effects after the process of intestinal digestive system. Funding Sources The work was supported by USDA.

scholarly journals Association of blood and liver free radical oxidation indicators in rats with different resistance to hypoxia after survived anoxia

2015 ◽  
Vol 96 (5) ◽  
pp. 798-802
Author(s):  
G A Bayburina ◽  
E A Nurgaleeva ◽  
S A Bashkatov ◽  
D Z Shibkova
Keyword(s):  
Antioxidant Activity ◽  
Factor Analysis ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Systemic Circulation ◽  
Antioxidant Systems ◽  
Radical Oxidation ◽  
Resistance To Hypoxia ◽  
White Rats

Aim. To study the association of rat liver and blood free radical oxidation indicators with different resistance to hypoxia on the long run after ischemic damage caused by systemic circulation arrest. Methods. Circulatory arrest lasting for 5 minutes was modeled at ether anesthesia on male white rats which were further allocated to 3 groups according to resistance to hypoxia. The observation period was 35 days. The levels of reduced glutathione, catalase, and products reacting with thiobarbituric acid were determined in liver homogenates, levels of erythrocyte catalase, total antioxidant activity, glutathione peroxidase, glutathione transferase, and products reacting with thiobarbituric acid - in blood. Statistical analysis was performed by parametric statistics, regression and factor analysis. Results. Rats with different resistance to hypoxia initially differed significantly on the number of safety systems functioning indicators levels in liver tissue and blood cells. Total factor analysis of the entire body of empirical data revealed two factors, conventionally called «resistance to hypoxia» and «antioxidant activity of blood». Daily analysis of the figures recorded at 1, 3, 5, 7, 14, 21 and 35 day of the experiment revealed similarities in common and daily factor solutions, demonstrating the objectivity of the revealed patterns, which emphasizes the significant role of free radical oxidation in anoxic conditions pathogenesis. Conclusion. The balance pro- and antioxidant systems activity in the liver and blood is an essential component of the body’s resistance to hypoxia affecting the survival of the animals after systemic circulation arrest. Their level predicts resistance to hypoxia at the recovery period.

scholarly journals Bioactive compounds content and total antioxidant activity of two savoy cabbages

2014 ◽  
Vol 32 (No. 6) ◽  
pp. 549-554 ◽  
Author(s):  
M.A. Fernández-León ◽  
M. Lozano ◽  
D. González ◽  
M.C. Ayuso ◽  
M.F. Fernández-León
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Fresh Weight ◽  
Total Antioxidant ◽  
In Vitro Antioxidant Activity

The bioactive compounds, as well as the in vitro antioxidant activity of two Savoy cabbage cultivars, Dama and Leticia, grown in west of Spain under similar conditions were identified, quantified and compared. We found that cv. Dama presented in general betters results when compared with cv. Leticia. Cv. Dama presented higher concentrations of chlorophyll a (2.26 mg/100 g fresh weight), total phenolic content (102.71 mg of chlorogenic acid equivalent/100 g fresh weight) and total intact glucosinolates (195.22 µmol of sinigrin equivalent/100 g fresh weight). Thus, cv. Dama exhibited higher values of in vitro antioxidant activity.  

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals Increasing Anthocyanin Content in Tomato Fruit for Improved Antioxidant Activity

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 869D-869
Author(s):  
Peter J. Mes* ◽  
James R. Myers
Keyword(s):  
Antioxidant Activity ◽  
Tomato Fruit ◽  
Introgression Line ◽  
Water Soluble ◽  
Carotenoid Content ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Tomato lines carrying the genes Aft, atv, Abg, hp-1, and an as yet undetermined gene from the introgression line LA2099 have been combined to produce fruit with elevated anthocyanin content. The antioxidant activity of juice made from anthocyanin-expressing tomatoes was compared to juices made from tomatoes with varied carotenoid content. The contribution of anthocyanin to the total antioxidant activity of the whole fruit in current material is small, but with potential for significant improvement. The increase in flavonoids in the elevated anthocyanin lines has increased water-soluble antioxidant activity of the fruit in vitro.

scholarly journals Studies on antioxidant activity of different extracts of aerial parts of Cadaba farinose Forsk: An in-vitro techniques

2020 ◽  
Vol 11 (3) ◽  
pp. 2986-2992
Author(s):  
Jambula Dinesh Babu ◽  
Venugopalan Santhosh Kumar
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
The Difference

Cadaba farinosa (family Capparidaceae) is generally known as “Indian cadaba” in the traditional ayurvedic system. The current study, aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Cadaba farinose was evaluated for its in-vitro antioxidant potential by Diphenylpicrylhydrazyl radical, and total antioxidant activity taking ascorbate as standard and superoxide radical activity taking Quercetin as the standard for the in-vitro methods. The methanolic concentrates of Cadaba farinose& ascorbic acid exhibited antioxidant potential possessing IC50 208µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical) , 188µg/ml & 57µg/ml (total antioxidant). The methanolic concentrates of Cadaba farinose & quercetin exhibited antioxidant potential possessing IC50 252µg/ml & 60µg/ml (superoxide radical). The IC50 value was originated that methanolic concentrates of Cadaba farinose more efficient in Diphenylpicrylhydrazyl radical, superoxide radical activity, total antioxidant activity compared EA & PE concentrates. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound present in different solvents. Invitro antioxidant studies show methanolic concentrates of Cadaba farinose have better antioxidant activity. This result indicates that aerial parts of methanolic concentrate Cadaba farinose could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

scholarly journals Studying the processes of free-radical oxidation in patients with purulent-inflammatory diseases of soft tissues

2015 ◽  
Vol 96 (3) ◽  
pp. 302-306
Author(s):  
T Z Zakiev ◽  
S R Tuysin ◽  
O V Galimov ◽  
A R Gil’fanov ◽  
R D Sagdeev
Keyword(s):  
Free Radical ◽  
Whole Blood ◽  
Soft Tissues ◽  
Local Treatment ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Radical Oxidation ◽  
Tissue Homogenates

Aim. To study the effects of combined dressings on the processes of free radical oxidation in patients with purulent wounds.Methods. The performance of luminol-dependent chemiluminescence of whole blood from 30 healthy donors and 122 patients with purulent wounds, as well as tissue chemiluminescence of festering wounds tissue homogenates were examined before and after the treatment. To assess the free radical oxidation in vitro, spontaneous and zymosan-induced whole blood chemiluminescence measurement, iron-induced chemiluminescence assessment of festering wounds tissue homogenates were performed. The distribution of chemiluminescence parameters compared to normal expected distribution was analyzed to group homogeneity on these criteria.Results. Group of donors was homogeneous in composition that allowed the calculation of mean values. By changing in whole blood chemiluminescence of patients with soft tissue festering wounds, they were allocated to two groups, in which a marked increase or decline of the investigated parameters was seen. Increased luminol-dependent blood chemiluminescence indicates excessive free radicals generation by phagocytes and is characteristic of acute inflammation. Decreased intensity of chemiluminescence was observed in the blood of patients who showed reduced functional activity of phagocytes, which, together with the clinical features indicate a long-term smoldering inflammation. The comparative assessment of «Poliderm», «Voskopran», «Polysorb», «Levomekol» medications, used for local treatment of suppurative wounds, influence on free radical oxidation in model systems in vitro, was performed.Conclusion. There is a triple increase in chemiluminescence intensity in the acute phase of the inflammatory process, marking increased production of reactive oxygen radicals with microbicide activity); in long-term smoldering processes, chemiluminescence decreases by half, indicating reduced efficiency of protective mechanisms

scholarly journals Screening of Selected Sri Lankan Seasonal Fruit Extracts for Total Antioxidant Activity in vitro

2019 ◽  
Vol 7 (1) ◽  
pp. 7-12
Author(s):  
Amarosige Shanoli Kavindya Fernando ◽  
Anoja Priyadarshani Attanayake ◽  
Kamani Ayoma Perera Wijewardena Jayatilaka
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Antioxidant Activity ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Fruit Extracts ◽  
Total Polyphenol Content ◽  
Total Antioxidant

The present study aimed to determine the total polyphenol content and total antioxidant activities of ten selected seasonal fruit extracts grown in Sri Lanka. The aqueous refluxed fruit extracts (4h) were used at the initial concentration of 0.05 g/mL. The total polyphenol content was determined according to the Folin-Ciocalteu method. The total antioxidant activity was evaluated by DPPH (2, 2-diphenyl-2-picrylhydrazyl), FRAP (ferric reducing power) and TBA (thiobabituric acid assay) assays with L-ascorbic acid as the reference compound. The total polyphenol content of the fruit extracts varied from 0.81±0 to 17.54±0.50 mg GAE (gallic acid equivalents) per gram of dry weight. The antioxidant activities ranged in IC50 of 46.60±0.60 to 367.90 ± 4.90 μg/mL, 3.41±0.02 to 50.46±1.02 µM, 30.62±0.50 to 182.64±0.90 mg AAE (L-ascorbic acid equivalents) per gram of dry weight for DPPH, FRAP, TBA assays, respectively. All aqueous fruit extracts exert dose dependent in vitro antioxidant activities in different degrees. Among the selected fruit extracts S. carophylatum, P. zeylanica, A. comosus exert relatively high total antioxidant activity together with high total polyphenol content. Hence, the selected fruit extracts are deserved to be as potent sources for antioxidant lead compounds in the development of nutraceuticals using the above edible fruits.

Sign in / Sign up

Export Citation Format

Share Document