scholarly journals Studying the processes of free-radical oxidation in patients with purulent-inflammatory diseases of soft tissues

2015 ◽  
Vol 96 (3) ◽  
pp. 302-306
Author(s):  
T Z Zakiev ◽  
S R Tuysin ◽  
O V Galimov ◽  
A R Gil’fanov ◽  
R D Sagdeev
Keyword(s):  
Free Radical ◽  
Whole Blood ◽  
Soft Tissues ◽  
Local Treatment ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Radical Oxidation ◽  
Tissue Homogenates

Aim. To study the effects of combined dressings on the processes of free radical oxidation in patients with purulent wounds.Methods. The performance of luminol-dependent chemiluminescence of whole blood from 30 healthy donors and 122 patients with purulent wounds, as well as tissue chemiluminescence of festering wounds tissue homogenates were examined before and after the treatment. To assess the free radical oxidation in vitro, spontaneous and zymosan-induced whole blood chemiluminescence measurement, iron-induced chemiluminescence assessment of festering wounds tissue homogenates were performed. The distribution of chemiluminescence parameters compared to normal expected distribution was analyzed to group homogeneity on these criteria.Results. Group of donors was homogeneous in composition that allowed the calculation of mean values. By changing in whole blood chemiluminescence of patients with soft tissue festering wounds, they were allocated to two groups, in which a marked increase or decline of the investigated parameters was seen. Increased luminol-dependent blood chemiluminescence indicates excessive free radicals generation by phagocytes and is characteristic of acute inflammation. Decreased intensity of chemiluminescence was observed in the blood of patients who showed reduced functional activity of phagocytes, which, together with the clinical features indicate a long-term smoldering inflammation. The comparative assessment of «Poliderm», «Voskopran», «Polysorb», «Levomekol» medications, used for local treatment of suppurative wounds, influence on free radical oxidation in model systems in vitro, was performed.Conclusion. There is a triple increase in chemiluminescence intensity in the acute phase of the inflammatory process, marking increased production of reactive oxygen radicals with microbicide activity); in long-term smoldering processes, chemiluminescence decreases by half, indicating reduced efficiency of protective mechanisms

scholarly journals The aspects of free radical oxidation in the lungs of rats with pleurisy under long-term lighting

2017 ◽  
Author(s):  
O. M. Larycheva ◽  
L. D. Chebotar ◽  
T. Ya. Yaroshenko
Keyword(s):  
Free Radical ◽  
Electron Transport ◽  
Pineal Gland ◽  
Light Exposure ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Ros Generation ◽  
Radical Oxidation ◽  
Carrageenan Pleurisy

Introduction.Pathological changes in human body due to light exposure at night are triggered by circadian disruption, inhibition of melatonin secretion by the pineal gland at night that leads to its reduction in blood. Clinical and experimental data indicate high importance of free radical oxidation inthe pathogenesis of pulmonary diseases. The aim of the study – to explore the influence of a melatonin deficiencyin addition to pleurisy on free radical oxidation in the tissues of the lungs of rats exposed to light for 30 days.Research Methods. Experiments were carried out on male albino Wistar rats. The animals were randomized into four groups with 7 animals in each: an intact group, 30 day pineal gland hypofunction, carrageenan pleurisy, 30 day pineal gland hypofunction with carrageenan pleurisy. Pineal gland hypofunction was modeled by 1500 lx fluorescent lamps around the clock. The experimental pleurisy was induced in anesthetized animals by intrapleural injection of 0.1 ml of carrageenan. Superoxide (•О2-) products were determined by the reaction with nitro blue tetrazolium under the influence of NADH, NADP and pirogenal. The concentration of diene conjugates was determined by I.D. Stalna technique, TBA-active products – by I.D. Stalna, T.H. Harishvili method, trienes, ketodienes, - by a modified method based on the molar extinction coefficient.Results and Discussion.Assessingthe intensity of the reactive oxygen species (ROS) generation, a significantincrease of the •О2-level in all sources of its generation in the tissues of the lungs has been observed both in the control group with pleurisy and in the rats with 30-day pineal gland hypofunctionandpleurisy, compared to the intact animals.The chronic pineal gland hypofunction in the animals with experimental pleurisy lead to •О2- generationby the microsomal electron transport chain (ETC) by 54.6 % (р<0.05) when compared with carrageenan group of rats. No significant changes in concentration of the lipid peroxidation products has been observed in the animals with pleurisy during long-term light exposure.Conclusions. Chronic hypofunction of the pineal gland with the carrageenan-induced pleurisy causes activation of the free radical processes in the rats’ lung tissues and occur as hyperproduction of the superoxide anion radical by the microsomal chain of oxidation. This fact denotes probable abnormalities in the function of the microsomal electron transport chains especially under the condition change of properties of dehydrogenases. 

Lipid Peroxidation in Iron-Overloaded Spleens

1981 ◽  
Vol 60 (3) ◽  
pp. 295-301 ◽  
Author(s):  
A. D. Heys ◽  
T. L. Dormandy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Iron Overload ◽  
Iron Content ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Therapeutic Implications ◽  
Dose Dependent ◽  
Increased Susceptibility

1. Iron-overloaded spleens from thalassaemic subjects showed a greatly increased susceptibility to free-radical oxidation (peroxidation) in vitro. 2. Iron content was the main but not the only variable governing susceptibility. Added ascorbate had a dose-dependent action either as an antioxidant or as a pro-oxidant, the direction of its effect depending mainly on the degree of iron overload. This has therapeutic implications.

scholarly journals The effect of electromagnetic radiation on indexes of antioxidant defense in rat erythrocytes and blood plasma

2021 ◽  
pp. 73-79
Author(s):  
Н.А. Терехина ◽  
А.Д. Селин ◽  
Г.А. Терехин
Keyword(s):  
Electromagnetic Field ◽  
Free Radical ◽  
Blood Plasma ◽  
Electromagnetic Radiation ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
Rat Erythrocytes ◽  
Radical Oxidation ◽  
Decimeter Range

Цель исследования - изучение влияния электромагнитного излучения дециметрового диапазона на показатели антиоксидантной защиты в эритроцитах и плазме крови крыс. Методика. Эксперименты выполнены на крысах находившихся под влиянием электромагнитного поля дециметрового диапазона в течение 3-х мес. Проведен хемилюминесцентный анализ эритроцитов и плазмы крови животных. Интенсивность свободно радикального окисления в эритроцитах и плазме крови оценивали по показателям: максимальной интенсивности хемилюминесценции (Imax); светосуммы (S); светосуммы после максимального значения хемилюминесценции S(imax); коэффициентам Zlmax и Dec. Спектрофотометрически определяли содержание восстановленного глутатиона в эритроцитах. В плазме крови определяли содержание церулоплазмина, меди и железа. Анализ этих показателей был проведен в динамике исследования через 30, 60 и 90 сут. Результаты. Длительное (в течение 3 мес) нахождение животных в условиях действия электромагнитного поля приводит к изменениям показателей хемилюминесценции эритроцитов. Изменение показателей Imaх, S, Simax свидетельствует о нарушении процессов свободнорадикального окисления. В эритроцитах периферической крови крыс при этом установлено повышение содержания глутатиона. В плазме крови выявлено увеличение концентрации церулоплазмина и меди, снижение содержания железа. Заключение. Длительное нахождение животных в условиях действия электромагнитного поля приводит к нарушению равновесия в прооксидантно-антиоксидантной системе в эритроцитах. Антиоксиданты глутатион и церулоплазмин являются мишенью для действия электромагнитного излучения дециметрового диапазона. Установлено влияние длительного электромагнитного излучения на показатели минерального обмена: снижение содержания железа и увеличение содержания меди в плазме крови. The aim of the research was to study the effect of decimeter-range electromagnetic radiation on indexes of antioxidant defense in rat erythrocytes and blood plasma. Methods. Experiments were performed on rats exposed to decimeter-range electromagnetic field for three mos. Chemiluminescent analysis of erythrocytes and blood plasma was performed. Intensity of free-radical oxidation in erythrocytes and plasma was evaluated by parameters of maximum chemiluminescence intensity (Imax), light sum (S), the light sum after the maximum chemiluminescence value (imax), and ZImax and Dec coefficients. The content of reduced glutathione in peripheral blood erythrocytes and the plasma contents of ceruloplasmin, iron and copper were measured spectrophotometrically at 30, 60, and 90 days. Results. A long-term exposure of animals to the electromagnetic field for 3 mos. led to changes in chemiluminescent parameters of erythrocytes. The changes in Imax, S, and Simax indicated impairment of free-radical oxidation processes. The content of glutathione was increased in peripheral blood erythrocytes. The plasma contents of ceruloplasmin and copper were increased whereas the content of iron was decreased. Conclusion. The long-term exposure of animals to the electromagnetic field results in an imbalance of the erythrocyte pro-/antioxidant system. The antioxidants glutathione and ceruloplasmin are targets for the decimeter-range electromagnetic radiation. The study demonstrated the effect of long-term electromagnetic radiation on indexes of mineral metabolism evident as a decrease in the content of iron and an increase in the content of copper in the blood plasma.

scholarly journals Putative anticancer potential of novel 4-thiazolidinone derivatives: cytotoxicity toward rat C6 glioma in vitro and correlation of general toxicity with the balance of free radical oxidation in rats

2016 ◽  
Vol 57 (2) ◽  
pp. 151-163 ◽  
Author(s):  
Lesya I. Коbylinska ◽  
Nataliya M. Boiko ◽  
Rostyslav R. Panchuk ◽  
Iryna I. Grytsyna ◽  
Olga Yu. Klyuchivska ◽  
...  
Keyword(s):  
Free Radical ◽  
Free Radical Oxidation ◽  
C6 Glioma ◽  
Radical Oxidation ◽  
General Toxicity ◽  
Rat C6 Glioma

Effects of methyluracil and hydroxymethacil on free-radical oxidation in model systems

1995 ◽  
Vol 120 (2) ◽  
pp. 785-788
Author(s):  
V. A. Myshkin ◽  
Z. G. Khaibullina ◽  
S. A. Bashkatov ◽  
V. P. Krivonogov ◽  
D. A. Enikeev
Keyword(s):  
Free Radical ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Radical Oxidation

scholarly journals EVALUATION OF ANTIOXIDANT ACTIVITY OF PLANT AGENTS

2020 ◽  
pp. 187-193
Author(s):  
Yekaterina Vladimirovna Ferubko ◽  
Valeriy Nikolayevich Zelenkov ◽  
Anatoliy Andreyevich Lapin ◽  
Tamara Darizhapovna Dargayeva
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Lipid Analysis ◽  
Free Radical Oxidation ◽  
Inhibitory Effect ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
White Rats ◽  
Total Antioxidant

The aim of the research is to determine the total antioxidant activity of in vitro collection of antihepatotoxic action consisting of roots and rhizomes of Inula helenium L., grass Centaurium erythraea Rafn., flowers Tanacetum vulgare L., fruits Rosa sp., fruits of Crataegus sp. аnd its separate components by the method of colonometric titration and determination of antioxidant activity of dry extract (conditional name "Pentafite") obtained from this collection under conditions of experimental tetrachloride of carbon hepatitis in white rats. A method of chemiluminescent lipid analysis was used to evaluate the free radical oxidation of lipids in liver in test animals. It has been found that collection of antihepatotoxic action and plants included in it have antioxidant activity in vitro, at the same time synergistic effect on manifestation of total antioxidant activity of collection is revealed. Pharmacotherapy of experimental hepatitis in experimental rats by administration of Pentafite at a dose of 300 mg/kg has been found to significantly reduce the chemiluminometric values of lipids, which characterize the intensity of free radical reactions. "Pentafite" has a pronounced inhibitory effect on hyperlipoperoxidation in animal liver in case of toxic damage to it. The established antioxidant activity of collecting antihepatotoxic action and Pentafite extract may be important for their use in the treatment and prevention of hepatobiliary diseases.

scholarly journals Effect of bromfenac on free radical oxidation in model systems

2019 ◽  
Vol 100 (4) ◽  
pp. 636-641 ◽  
Author(s):  
E F Galimova ◽  
Z G Khaibullina ◽  
D A Enikeev ◽  
Yu L Bortsova ◽  
K S Mochalov ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Reactive Oxygen ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Oxygen Species ◽  
Radical Oxidation ◽  
Anti Inflammatory

Aim. The study of free radical oxidation processes in an experiment on model systems using the anti-inflammatory drug bromfenac (nakwan) widely used in ophthalmology for the treatment of infectious and inflammatory diseases of the anterior chamber of the eye. Methods. The antioxidant capacity of the drug was evaluated by chemiluminescence registration and analysis of model systems that generate reactive oxygen species and reproduce lipid peroxidation processes using the chemiluminomer CL-003. The following parameters of spontaneous and induced chemiluminescence were determined: light sum and maximum luminescence amplitude, duration of latent period, amplitude of fast and slow flash. Results. When tested in vitro in two different model systems, a high antioxidant activity of the studied drug was established, up to complete suppression of chemiluminescence when 90 μg of bromfenac was added to the incubation medium, which characterizes the inhibition of the generation of reactive oxygen species. A significant increase in total antioxidant activity with bromfenac was also demonstrated, which is reflected by the integral parameter of chemiluminescence — light sum which decreased with the introduction of 10 μg of the drug by 1.2 times, and with 90 μg by 1.5 times. A comparative analysis of the antioxidant properties of various nonsteroidal anti-inflammatory drugs used in ophthalmic practice demonstrated a more pronounced efficacy of bromfenac compared to ketorolac, the use of which was not accompanied by statistically significant changes in chemiluminescence. A very important mechanism of the positive effect of bromfenac is the direct dependence of the action on its quantity in the reaction medium, which opens up prospects for the controlled correction of free radical phenomena and the excessive activation of lipid peroxidation in the imbalance of the pro- and antioxidant processes in biological systems. Conclusion. It is suggested that the protective effects of the drug in various infectious-inflammatory lesions of the eye can be determined, along with previously known properties, its antioxidant activity, restriction of increased production of reactive oxygen species and oxidative stress phenomena.

scholarly journals The effect of thyroxine isomers on the processes of free radical oxidation in the subcellular fractions of the rat cerebral cortex

2000 ◽  
Vol 46 (4) ◽  
pp. 32-34
Author(s):  
O. V. Galkina ◽  
V. M. Prokopenko ◽  
F. E. Putilina ◽  
N. D. Yeshchenko ◽  
A. V. Arutyunyan
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Mitochondrial Fraction ◽  
Rat Cerebral Cortex ◽  
Radical Oxidation ◽  
Adult Rat ◽  
Synaptosomal Fraction ◽  
The Brain

Effective concentrations for D and L thyroxin isomers were determined by the chemiluminescent (CL) method and their effects on free radical oxidation in the mitochondrial and synaptosomal fraction of adult rat cerebral cortex were studied in vitro. A OA DT4 in a model system with riboflavin was 2.2 times higher than L-T4. Effective concentrations for both thyroxin forms were 1$q= = 7.43 x 10~5+/-0.71 M for D-T4 and 15q=15.47 x 10~5+/1.23 M for L-T4. Thyroxin effect on membranous fraction of the brain cortex was studied in vitro using luminol-dependent peroxide CL. In normal concentrations (1 x 108 M) both hormone forms exerted equally intensive antioxidant effect which was more pronounced in the mitochondrial fraction, where CL decreased by 69 and 66%, while in the synaptosomal fraction it decreased only by 45 and 46%. Since D form possesses no hormonal activity, this effect may be due to phenol origin of thyroxin.  

scholarly journals Comparative assessment of antioxidant properties of polyphenols from heartwood and Maackia amurensis callus culture

2010 ◽  
Vol 9 (1) ◽  
pp. 17-20 ◽  
Author(s):  
O. V. Azarova ◽  
V. M. Bryukhanov ◽  
V. P. Bulgakov ◽  
Ya. F. Zverev ◽  
V. V. Lampatov ◽  
...  
Keyword(s):  
Free Radical ◽  
Callus Culture ◽  
Oxidant Stress ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
Comparative Assessment ◽  
Prooxidant Activity ◽  
Radical Oxidation ◽  
Maackia Amurensis

On long-term introduction to rats in dosage 100 mg/kg polyphenol complexes from heartwood and callus culture of Maackia amurensis Rupr. et Maxim. had a marked influence on the activity of free radical oxidation. Under conditions of induced oxidant stress both polyphenol complexes inhibited prooxidant activity directly affecting the products of free radical oxidation. Inhibiting influence on the production of oxygen active forms that is characteristic for both phytocomplexes is determined by inhibition of mechanisms both enzymatic and non-enzymatic defense.

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