scholarly journals Potassium solubilization in phonolite rock by diazotrophic bacteria

2017 ◽  
Vol 8 (1) ◽  
pp. 17 ◽  
Author(s):  
Ligiane Aparecida Florentino ◽  
Adauton Vilela Rezende ◽  
Cássia Cristina Bachião Miranda ◽  
Aline Carvalho Mesquita ◽  
José Ricardo Mantovani ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ph Value ◽  
Carbon Sources ◽  
Control Treatment ◽  
Nitrogen Fixing ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Nitrogen Fixing Bacteria ◽  
Rock Powder ◽  
Potential Use

Some strains of nitrogen fixing bacteria, besides fixing the N2, can produce phytohormones, control pathogens, act in solubilization of minerals containing phosphorus (P) and potassium (K), contributing to plant growth. However, the solubilization of minerals containing K has been little exploited. Therefore, the aim of this study was to evaluate potassium solubilization (K) of phonolite rock by strains of associative nitrogen fixing bacteria supplied with two different carbon sources. Twelve bacterial strains were cultured for seven days at 25°C in Aleksandrov medium supplemented with phonolite rock powder. The experiment was carried out in a completely randomized factorial design, 13 x 2 (12 bacterial strains and a control without inoculation) and two carbon sources (glucose and sucrose) with four repetitions. After the growth, the supernatant was separated by centrifugation and analyzed for the final pH value and the content of K. All diazotrophic bacteria strains contributed to increase the release of K when compared to the control treatment. The strain UNIFENAS 100-94 solubilized 130 mg L-1 K in the presence of the two carbon sources, indicating the potential use of these diazotrophic bacterial strains for K solubilization from minerals.

scholarly journals Bacterial strains decrease solubility of potassium in the soil

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Mario Viana Paredes Filho ◽  
Aline Carvalho Mesquita ◽  
José Ricardo Mantovani ◽  
Adauton Vilela de Rezende ◽  
Ligiane Aparecida Florentino
Keyword(s):  
Ph Value ◽  
Control Treatment ◽  
Bacterial Strains ◽  
Retention Capacity ◽  
Rock Powder ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Soluble Fertilizer ◽  
Potential Acidity

Current study verifies whether inoculation with strains of diazotrophic bacteria, with proven ability to solubilize potassium (K) in vitro, contributes towards the release of K in the soil after fertilization with phonolite rock powder. The experiment was conducted in containers with 0.3 dm-3 of soil containing low potassium content. Fifteen treatments were used, namely, 12 inoculated with the bacterial strains, a control treatment (without phonolite and without inoculation), one containing phonolite without inoculation and one containing KCl, soluble fertilizer, without inoculation. In treatments with phonolite and KCl, the doses of these materials were applied to provide the soil with 195 mg dm-3 of K. A completely randomized design with four replications was used. The soil was incubated during 90 days at room temperature and humidity at about 70% retention capacity. After this period, the content of K+ (Mehlich and resin), pH value and potential acidity (H+Al) were evaluated. Phonolite, associated with inoculation with most bacterial strains, increased the availability of potassium in the soil, pH rate and reduced potential acidity. Among the strains tested, UNIFENAS 100-01, UNIFENAS 100-16, UNIFENAS 100-27, UNIFENAS 100-39 and UNIFENAS 100-93 were the most efficient for the solubilization of K+ of the phonolite. In spite of the observed results, K content released by the bacterial strains in the soil decreased when compared to in vitro conditions, thus justifying the need for studies on bio-solubilization of soil to select the most efficient strains in the process.

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

Molecular Insights into Beneficial Effects of Tea-Plant Growth and Selenium Enrichment by Herbaspirillum camelliae

2021 ◽  
pp. 37-51
Author(s):  
Wei Cheng ◽  
Xuejing Yu ◽  
Xingguo Wang
Keyword(s):  
Plant Growth ◽  
Molecular Mechanisms ◽  
Acc Deaminase ◽  
Nitrogen Sources ◽  
Endophytic Bacterium ◽  
Tea Plant ◽  
Nitrogen Fixing ◽  
Biotic And Abiotic Stress ◽  
Bacterial Strains ◽  
Secretion Systems

Herbaspirillum camelliae WT00C, as a tea-plant endophytic bacterium, not only colonizes specifically in tea plants but also promotes tea-plant growth and selenium enrichment. Different from diazotrophic endophytes H. seropedicae, H. frisingense and H. rubrisubalbicans, H. camelliae WT00C does not display nitrogen-fixing activity. To understand the molecular mechanisms of promoting the growth of tea plant and Se-enrichment, we sequenced and annotated the genome of H. camelliae WT00C. The results showed that the genome was composed of 6,079,821 base pairs with a total of 5,537 genes. The genomic survey also revealed that H. camelliae WT00C was a multifunctional bacterium metabolizing a variety of carbon and nitrogen sources and defending against biotic and abiotic stress. Although this bacterium did not have intact nitrogen-fixing genes, its genome held the genes responsible for indole-3-acetic acid (IAA) biosynthesis, 1-aminocyclopropane-1-carboxylate (ACC) deamination, siderophore synthesis, ammonia formation, urea metabolism, glutathione and selenocompound metabolisms. Biosynthesis of IAA, siderophore, ammonia, urea and ACC deaminase could explain why two bacterial strains promote tea-plant growth and development. Selenocompound metabolism in this bacterium might also benefit tea-plant growth and Se-enrichment. In addition, the genome of H. camelliae also contained a multitude of protein secretion systems T1SS, T3SS, T4SS and T6SS, in which T4SS did not exhibit in other members of the genus Herbaspirillum.

scholarly journals Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

2019 ◽  
Vol 13 (2) ◽  
pp. 269-278
Author(s):  
Adriano Bortolotti Silva ◽  
Ligiane Aparecida Florentino ◽  
Dalvana De Sousa Pereira ◽  
Paulo Roberto Correa Landgraf ◽  
Ana Carolina Rodrigues Alves ◽  
...  
Keyword(s):  
Shoot Growth ◽  
Ex Vivo ◽  
Control Treatment ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Indole 3 Acetic Acid

Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

Endophytic nitrogen fixation – a possible ‘hidden’ source of nitrogen for lodgepole pine trees growing at unreclaimed gravel mining sites

2019 ◽  
Vol 95 (11) ◽  
Author(s):  
Kiran Preet Padda ◽  
Akshit Puri ◽  
Chris Chanway
Keyword(s):  
Nitrogen Fixation ◽  
Lodgepole Pine ◽  
Nifh Gene ◽  
Control Treatment ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Mining Sites ◽  
Greenhouse Study ◽  
Gravel Mining ◽  
Pine Trees

ABSTRACT Lodgepole pine (Pinus contorta var. latifolia) trees have been thriving on unreclaimed gravel mining sites in British Columbia, Canada, with tissue nitrogen-content and growth-rate unaffected by extremely low soil nitrogen-levels. This indicates that pine trees could be accessing a hidden nitrogen source to fulfill their nitrogen requirements – possibly via endophytic nitrogen-fixation. Endophytic bacteria originally isolated from native pine trees growing at gravel sites were selected (n = 14) for in vitro nitrogen-fixation assays and a year long greenhouse study to test the overall hypothesis that naturally occurring endophytic nitrogen-fixing bacteria sustain pine tree growth under nitrogen-limited conditions. Each of the 14 bacteria colonized the internal tissues of pine trees in the greenhouse study and fixed significant amounts of nitrogen from atmosphere (23%–53%) after one year as estimated through 15N isotope dilution assay. Bacterial inoculation also significantly enhanced the length (31%–64%) and biomass (100%–311%) of pine seedlings as compared to the non-inoculated control treatment. In addition, presence of the nifH gene was confirmed in all 14 bacteria. Our results support the possibility that pine trees associate with nitrogen-fixing bacteria, capable of endophytic colonization, to survive at unreclaimed gravel mining pits and this association could potentially be utilized for effective reclamation of highly disturbed sites in a sustainable manner.

Research on Improvement of Nitrogenase Activity of Free-Living Nitrogen-Fixing Bacteria Using DBD Plasma

2013 ◽  
Vol 671-674 ◽  
pp. 2674-2678 ◽  
Author(s):  
Yan Yun Zhu ◽  
Xiao Li Zhu ◽  
Fang She Yang
Keyword(s):  
Rhizosphere Soil ◽  
Barrier Discharge ◽  
Nitrogenase Activity ◽  
Nitrogen Fixing ◽  
Bacterial Strains ◽  
Dbd Plasma ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Reduction Assay ◽  
Rrna Sequencing

Nitrogen-fixing bacteria were screened from the rhizosphere soil of plants in Shaanxi in China. 36 free-living nitrogen-fixing bacterial strains were isolated and their nitrogenase activity were determined by acetylene reduction assay (ARA), two strains named FLNB03 and FLNB09 with higher nitrogenase activity were isolated and identified by 16S rRNA sequencing. The datum showed that FLNB03 was similar to Acinetobacter and their similarity reached 99%, FLNB09 was similar to Agrobacterium sp. and their similarity reached 99%. Then both of them were treated using Dielectric Barrier Discharge (DBD) plasma for mutation and their mutants called FLNB03-2 and FLNB09-3 were obtained. The nitrogenase activity of FLNB03-2 was 0.61±0.10 nmol•107cfu-1•h-1, and that of FLNB09-3 was 0.40±0.05 nmol•107cfu-1•h-1, their nitrogenase activity increased by 22.00% and 14.29% than their original bacteria respectively. FLNB03-2 and FLNB09-3 might be used as microbial fertilizer.

Polyhydroxyalkanoate analysis inAzospirillum brasilense

1995 ◽  
Vol 41 (13) ◽  
pp. 73-76 ◽  
Author(s):  
Robin Itzigsohn ◽  
Oded Yarden ◽  
Yaacov Okon
Keyword(s):  
Azospirillum Brasilense ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Dry Mass ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Qualitative And Quantitative ◽  
Alkanoic Acids ◽  
Quantitative Analyses

The considerable industrial interest in the qualitative and quantitative production of polyhydroxyalkanoates in microorganisms has led to the characterization of those synthesized in the nitrogen-fixing bacteria Azospirillum brasilense and Azotobacter paspali. In contrast to some other bacterial species, Azospirillum brasilense does not produce copolymers of hydroxyalkanoates when grown under the different carbon sources assayed, namely n-alkanoic acids, hydroxyalkanoates, and sugars with varying C:N ratios. Rather, only homopolymers of polyhydroxybutyrate were detected, comprising up to 70% of the cell dry mass. No copolymers were detected in Azotobacter paspali. Quantitative analyses of poly(β-hydroxybutyrate) are also presented.Key words: Azospirillum spp., Azotobacter paspali, polyhydroxyalkanoate analysis, PHA, PHB.

In vitro inhibition of nonsymbiotic nitrogen-fixing bacteria by rhizosphere actinomycetes associated with grasses

1982 ◽  
Vol 28 (6) ◽  
pp. 705-709 ◽  
Author(s):  
D. A. Zuberer ◽  
Mark Roth
Keyword(s):  
Antibiotic Production ◽  
Nitrogen Fixing ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Free Living ◽  
Vitro Assay ◽  
Genus Streptomyces ◽  
Central Texas ◽  
Actinomycete Isolate

Eighty-five isolates of actinomycetes were obtained from the rhizospheres of 10 indigenous grasses of central Texas. Of these, 10 were classified as members of the genus Nocardia; the remainder were members of the genus Streptomyces. To determine whether antibiotic production might be a significant factor in the establishment of associative symbioses between grasses and diazotrophic bacteria, 12 strains of free-living nitrogen-fixing bactria were cross streaked against each actinomycete isolate. This in vitro assay indicated that 78% of the 85 isolates were antagonistic to at least one of the 12 nitrogen-fixing bacterial strains.

Nitrogen-fixing bacteria with multiple plant growth-promoting activities enhance growth of tomato and red pepper

2013 ◽  
Vol 53 (12) ◽  
pp. 1004-1015 ◽  
Author(s):  
Md. Rashedul Islam ◽  
Tahera Sultana ◽  
M. Melvin Joe ◽  
Woojong Yim ◽  
Jang-Cheon Cho ◽  
...  
Keyword(s):  
Plant Growth ◽  
Red Pepper ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Nitrogen-Fixing and Plant Growth-Promoting Ability of Two Endophytic Bacterial Strains Isolated from Sugarcane Stalks

Sugar Tech ◽  
2015 ◽  
Vol 18 (4) ◽  
pp. 373-379 ◽  
Author(s):  
Yong-Xiu Xing ◽  
Chun-Yan Wei ◽  
Yao Mo ◽  
Li-Tao Yang ◽  
Si-Liang Huang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nitrogen Fixing ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
Growth Promoting
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