scholarly journals Titanium dioxide stimulates phagocytic activity of blood neutrophils

2017 ◽  
pp. 58-64
Author(s):  
N Aliakhnovich
Keyword(s):  
Staphylococcus Aureus ◽  
Bronchial Asthma ◽  
Metabolic Activity ◽  
Phagocytic Activity ◽  
Physiological Solution ◽  
Healthy People ◽  
Atopic Bronchial Asthma ◽  
Neutrophil Activity ◽  
Nbt Test ◽  
Blood Neutrophils

The aim. Evaluation of the TiO2 effect on the phagocytic activity of peripheral blood neutrophils. Materials and methods. 17 people were examined - 10 people with atopic bronchial asthma (m/f - 5/5, mean age - 38 [28, 48]), verified according to the international criteria, the second group (control) - 7 people - healthy controls (m/f - 3/4, mean age - 23 [21; 26] years). The phagocytic index and neutrophil activity in the NBT-test were calculated for all the examined patients. 0.008% solution of TiO2 (based on 1 mg / kg of body weight) was added to the test-samples, control samples were added with the physiological solution, standard samples - with a suspension of killed Staphylococcus aureus. All the samples were incubated for 30 minutes at 37°C, the ready smears were microscopied with counting the amount of formazan-positive granulocytes in the NST-test and cells that absorbed 1 or more Stf particles per 100 granulocytes. Results. TiO2 stimulated phagocytosis of staphylococcus with blood neutrophils in 53% of the examined patients by 8-30% (p = 0.02). Under the influence of TiO2 activity of phagocytes significantly increased, in comparison with that in spontaneous NST-test (p = 0.0001), but was lower than induction by staphylococcus (p = 0.049). In patients with allergopathology, TiO2 stimulated phagocytosis of staphylococcus and neutrophil activity in the NBT-test was also effective, as was the culture of staphylococcus aureus (31.2% compared to 34.3%). In the healthy group, the TiO2 solution increased the activity of neutrophils in the NST-test, compared with the physiological solution (on average and in 71% of cases by 10-18%), but not so than the culture of staphylococcus (24%, compared to 39, 7%). Among healthy people, there was a correlation between the hereditary of allergy and the neutrophil activity in the NBT-test with TiO2 (0.78). Conclusions. It was found that 0.008% solution of TiO2 stimulated the activity of neutrophils in the NBT-test, both in patients with atopic bronchial asthma and healthy people after 30 minutes of incubation in a thermostat. In patients with allergies, TiO2 increased phagocytosis of staphylococcus, as well as metabolic activity of neutrophils so much as staphylococcus. A strong correlation was found between the hereditary allergies and the increased metabolic activity of blood phagocytes in the NBT-test with TiO2 in healthy individuals.

scholarly journals FUNCTIONAL PROPERTIES OF BLOOD NEUTROPHILS AND PERITONEAL EXUDATE IN PATIENTS WITH PERITONITIS

2019 ◽  
pp. 22-29
Author(s):  
A. Stasenko
Keyword(s):  
Functional Properties ◽  
Metabolic Activity ◽  
Abdominal Cavity ◽  
The Body ◽  
Peritoneal Exudate ◽  
Innate Defense ◽  
Local Protection ◽  
Cellular Factors ◽  
Nbt Test ◽  
Blood Neutrophils

The aim of the study is to investigate the functional properties of neutrophils in the blood and peritoneal exudate in patients with peritonitis. Materials and methods. The metabolic activity of neutrophils determined in patients with peritonitis and in patients of the control groups in the blood, peritoneal exudate and effusion of the abdominal wall on the 1–2 day of the postoperative period in the Sp and St test with nitro blue tetrazolium (NBT), as well as reserve indicator (RI) of metabolic activity. We studied the percentage of active neutrophils in the phagocytosis –PhI ( index) and the number of absorbed Candida albicans particles on average by one neutrophil –PhN(number). Results and their discussion.The peculiarities of the cellular factors of local protection decreased metabolic activity of neutrophils in the abdominal cavity in the Sp NBT test compared to blood neutrophils and an increased reserve during stimulation. For the comparisons which included the activity of neutrophils abdominal contents in patients with peritonitis versus in control established that neutrophils in the abdominal cavity with peritonitis have a reduced intensity of phagocytosis. Patients with pancreatic peritonitis have inverse correlations between the amount of NBT-positive blood NG in the SP NBT test and IR of blood NG and between the amount of NBT-positive blood NG in the Sp NST test and PhI of blood NG, while patients with abdominal peritonitis do not have correlations between indicators of innate defense of the body. Conclusions. Peritonitis was associated with functional disorders of NG. The leading areas of disorders are significant activation of metabolic processes of NG and inhibition of the phagocytic function of NG blood and peritoneal exudate. Patients with pancreatic peritonitis had inverse correlations between the indicators of the innate defense of the body, while patients with abdominal peritonitis did not have correlations.

scholarly journals ASSESSMENT OF INDICES OF NONSPECIFIC RESISTANCE OF THE ORGANISM IN CONDITIONS OF NARROWING OF THE LUMEN OF THE TRACHEA IN AN EXPERIMENT

2018 ◽  
Vol 3 (4) ◽  
pp. 114-119
Author(s):  
I. S. Kurganskiy ◽  
S. A. Lepekhova ◽  
L. V. Zaritskaya ◽  
E. V. Batunova ◽  
V. N. Makhutov ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
White Male ◽  
Early Postoperative Period ◽  
The State ◽  
Nonspecific Resistance ◽  
Nbt Test ◽  
Blood Neutrophils ◽  
Male Wistar Rats ◽  
Original Procedure ◽  
System Assessment

The narrowing of the lumen of the trachea due to cicatricial stenoses of the trachea is one of the urgent problems of the modern surgery.  The processes taking place in the trachea and lungs lead to a change in the state of the immune system. Assessment of the dynamics of  indices of nonspecific resistance is one of the methods for assessing  the severity of the course of the disease. We studied the indices of  nonspecific resistance of the organism in narrowing the lumen of the  trachea in an experiment in the early postoperative period.  The study was carried out on the basis of the scientific department  of experimental surgery with the vivarium of Irkutsk Scientific  Centre of Surgery and Traumatology, on 24 white male Wistar rats  with a body weight of 300–350 g. We simulated narrowing the lumen of the trachea by placing a silicone tube in the lumen of the trachea  for 14 days. The parameters of phagocytosis, the state of phagocytic activity of blood neutrophils, spontaneous NBT-test, induced NBT- test were studied. The animals were withdrawn from the experiment  on the 3rd, 7th and 14th day. We revealed that as a result of  narrowing of the lumen of the trachea with the original procedure,  the nonspecific resistance of the organism was violated with  inhibition of the phagocytic activity of leukocytes. There was  inhibition of phagocytic activity of leukocytes and depletion of the  functional reserve of leukocytes.

Taban-arshan: Immunocorrector in atopic bronchial asthma

2004 ◽  
Vol 138 (7) ◽  
pp. 65-66 ◽  
Author(s):  
L. Yu. Semenova ◽  
Zh. M. Salmasi ◽  
A. N. Kazimirskii ◽  
G. V. Poryadin
Keyword(s):  
Bronchial Asthma ◽  
Atopic Bronchial Asthma

PREVALENCE OF PNEUMOCYSTIS JIROVECII IN FREQUENTLY ILL CHILDREN ON ADMISSION TO THE RESPIRATORY DEPARTMENT AND DURING SANATORIUM REHABILITATION

2021 ◽  
Vol 100 (2) ◽  
pp. 104-111
Author(s):  
L.V. Feklisova ◽  
◽  
N.V. Karazhas ◽  
M.K. Khadisova ◽  
E.I. Likhanskaya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Active Form ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Pharyngeal Wall ◽  
Blood Neutrophils ◽  
Pneumocystis Infection ◽  
Chain Fatty Acids

The aim of the study was to determine the frequency of occurrence and to assess the significance of markers of pneumocystosis in frequently ill children (FIC) during inpatient treatment and during rehabilitation in a sanatorium. Materials and methods of research: 115 children with recurrent respiratory diseases were observed: 52 were treated in a hospital and 63 underwent rehabilitation in a sanatorium. Biological samples (oropharyngeal swabs, saliva, feces and blood serum) were examined for pneumocystosis. Laboratory techniques used: a polymerase chain reaction (PCR) to detect DNA pathogens, an indirect immunofluorescence reaction (NRIF) – antigens, and immunoforment analysis (IFA) – specific antibodies of immunoglobulins (Ig) of classes M (IgM) and G (IgG). The material for laboratory research was smears from the posterior pharyngeal wall (PCR, NRIF) and blood serum (IFA). Microbiological studies included seeding, isolation of cultures with quantitative counting of colony-forming units in samples of oropharyngeal swabs, feces. Determination of the functional activity of the oropharynx microbiocenosis by the concentration of short-chain fatty acids in saliva by means of GLC method. Phagocytic activity of blood neutrophils was also investigated. Results: in hospital patients, markers for pneumocystis were found in 60% of cases, including the active form (40%) and pneumocystis (13%); in the sanatorium – 33% of cases, with active infection (26%) and detection of pneumocystis (17%). Inpatients were diagnosed with clinical variants of pneumocystis infection: pneumonia, obstructive bronchitis, acute respiratory infections; schoolchildren have been diagnosed with active pneumocystis infection and carriage. Dysbiotic disorders of the loci of the oropharynx and intestines were identified in all examined subjects, inhibition of the phagocytic activity of blood neutrophils and shifts in the concentration of short-chain fatty acids have been observed. Conclusion: a high proportion of active pneumocystis infection (40% and 27% of cases) was revealed in FIC treated in the hospital and in the sanatorium, which represents a risk group for infection/reinfection with pneumocystosis in the premises.

scholarly journals Characteristics of the eosinophilic fenotype of bronchial asthma in children

2017 ◽  
pp. 52-57
Author(s):  
E Asiryan ◽  
N Titova
Keyword(s):  
Bronchial Asthma ◽  
Skin Tests ◽  
Control Group ◽  
Ige Receptor ◽  
Positive Skin ◽  
Ige Antibodies ◽  
Total Ige ◽  
Atopic Bronchial Asthma ◽  
Ige Receptors ◽  
High Level

The purpose of the work is clinical and laboratory examination of children with an eosinophilic phenotype of bronchial asthma. Materials and methods. 160 children aged 5 to 18 years were examined: 130 patients with atopic bronchial asthma, 30 children of the control group. During the work, the level of eosinophils, the level of eosinophils carrying FcεRI+ and CD23+IgE+ receptor, the level of total IgE, IgE antibodies to domestic and epidermal allergens in the blood serum were determined, clinical and anamnestic data were studied. Results. In children with atopic bronchial asthma, the relative and absolute content of eosinophils, as well as eosinophils carrying FcεRI+ and CD23+IgE+ receptors, is statistically significantly higher than in the control group (p<0.001). In 71 (54.62%) children with bronchial asthma, eosinophilia was detected, with 58 (81.69%) patients having a high level of eosinophils bearing the FcεRI + receptor and 56 (78.87%) children with a high level of eosinophils carrying CD23+IgE+ receptor. In the group of children with bronchial asthma, a correlation was established between the level of total IgE and the level of eosinophils carrying FcεRI+ (r=+0.389; p<0.01) and CD23+IgE+ receptors (r=+ 0.677; p<0.0001). In children with eosinophilia, in most cases, the hereditary nature of the disease is observed, the first manifestations of allergy are already present in the first year of life, concomitant allergopathology has been established. The optimal effect in the treatment of patients with eosinophilia was obtained with the appointment, as a basic therapy, of inhaled glucocorticosteroids, as well as short-acting β2-agonists for arresting episodes of bronchial obstruction. In most patients with asthma who have eosinophilia, serum levels of IgE antibodies to Dermatophagoides pteronyssinus, to the coat of the cat and the dog's coat are revealed, and positive skin scarification tests with these allergens are detected. Conclusion. In children with atopic bronchial asthma, there are signs of an eosinophilic phenotype: eosinophilia, high expression of FcεRI+ and CD23+IgE+ receptors, increased levels of total IgE and IgE antibodies, positive skin tests with domestic and epidermal allergens, hereditary predisposition, effectiveness of inhaled glucocorticosteroids.

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