scholarly journals THE CONCENTRATION OF INTERLEUKIN-6 AND INTERLEUKIN-8 IN HUMAN SEMEN WITH HIGH VISCOSITY

2021 ◽  
pp. 29-39
Author(s):  
D. Y. Sosnin ◽  
K. R. Gal'kovich ◽  
A. V. Krivtsov
Keyword(s):  
Seminal Plasma ◽  
Interleukin 6 ◽  
Comparison Group ◽  
Large Range ◽  
High Viscosity ◽  
Main Group ◽  
Interleukin 8 ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Range Of Values

Objective: to estimate the effect of ejaculate consistency on the levels of interleukin-6 and interleukin-8 in human seminal plasma. Material and methods. The concentration of interleukin-6 and interleukin-8 was determined by ELISA using the kit manufactured by «Vector-Best» (Russia). The study included 64 men: the main group (n = 30) presents patients with high semen viscosity, the comparison group (n = 34) presents men with normal semen viscosity. Results. In average, interleukin-6 level in the semen was 13.45 pg/ml, the median was 13.79 pg/ml; the data ranged from 8.24 pg/ml to 19.34 pg/ml. In average, level of interleukin-8 was 28.9 pg/ml, the median – 13.96 pg/ml; there is a large range of values from 0.202 pg/ml to 174.5 pg/ml. There are no significant differences in the values of interleukin-6 and interleukin-8 of the main group from the comparison group: for interleukin-6, U = 377.0 (p = 0.074655); for interleukin-8, U = 407.0 (p = 0.863852). The data obtained did not correlate neither between groups nor with the fertility markers of the human semen.<br>Conclusion. Interleukin-6 and interleukin-8 levels in the human seminal plasma do not depend on semen viscosity.

scholarly journals Anovel Immunological Technique for Identification of Human Seminal Fluid

2010 ◽  
Vol 7 (2) ◽  
pp. 1023-1027
Author(s):  
Baghdad Science Journal
Keyword(s):  
Seminal Plasma ◽  
Human Body ◽  
Seminal Fluid ◽  
Cross Reactivity ◽  
Body Fluids ◽  
Vaginal Fluid ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Ear Wax ◽  
Immunological Technique

An immunological technique was investigated for the detection of human semen in forensic analysis.This technique included a preparation of anti-human seminal plasma antibodies, by immunizing rabbits with treated human semen. The human semen was treated with an acid to prevent cross reactivity with other human body fluids. The antibody produced was tested against different animal,s seminal fluid samples (dog, goat ,sheep, cow) and human body fluids( saliva, blood , vaginal fluid, ear wax and human semen). It was found that using this developed technique was only selectively responsed with human semen . The prepered kit was evaluated and tested in Forensic laboratory- Ministry of Health. Finally, results were obtained in a comparison with the recommended techniques.

scholarly journals Resistin correlates with elastase and interleukin-6 in human seminal plasma

2008 ◽  
Vol 16 (2) ◽  
pp. 283-288 ◽  
Author(s):  
Juergen Kratzsch ◽  
Uwe Paasch ◽  
Sonja Grunewald ◽  
Marc Alexander Mueller ◽  
Joachim Thiery ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Interleukin 6 ◽  
Human Seminal Plasma

Fibrinogen-Like Substance and Thrombin-Like Enzyme in Human Seminal Plasma: Coagulation System of Human Semen

1997 ◽  
Vol 38 (1) ◽  
pp. 29-36 ◽  
Author(s):  
J.-Y. Park ◽  
Y. Yoshimura ◽  
S. Nozawa ◽  
T. Umeda ◽  
S. Akihama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Coagulation System ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Plasma Coagulation

Increased levels of interleukin-8 in human seminal plasma

Andrologia ◽  
2009 ◽  
Vol 30 (6) ◽  
pp. 339-343 ◽  
Author(s):  
E. Koumantakis ◽  
I. Matalliotakis ◽  
D. Kyriakou ◽  
Y. Fragouli ◽  
K. Relakis
Keyword(s):  
Seminal Plasma ◽  
Interleukin 8 ◽  
Human Seminal Plasma

The heat production of human spermatozoa and seminal plasma; with comparative observations on bull semen

1960 ◽  
Vol 152 (948) ◽  
pp. 298-310 ◽  
Keyword(s):  
Seminal Plasma ◽  
Heat Production ◽  
Human Spermatozoa ◽  
Enzymatic Reactions ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Pronounced Difference ◽  
Bull Semen ◽  
Isothermal Calorimeter ◽  
Aerobic And Anaerobic

The aerobic and anaerobic heat production of human semen, spermatozoa and seminal plasma has been examined in an isothermal calorimeter, capacity 2.4 ml., working at atmospheric pressure. The heat production of bull semen, spermatozoa and seminal plasma was also measured, for comparison. The anaerobic heat production of human spermatozoa (in semen), per unit number of cells, is markedly higher than that of bull spermatozoa (in semen). The ratio may reach 4:1. Up to two-thirds of the heat production of human semen originates in the seminal plasma. The contribution of seminal plasma to the heat production of bull semen is insignificant. The anaerobic heat production of human seminal plasma is more than fifty times as great as that of bull seminal plasma. When anaerobic bull semen is aerated by mixing it in the calorimeter with an aerated diluent, there is a sudden burst of heat production. The same phenomenon occurs when human semen is mixed with an aerated diluent, though the effect is less marked. It is concluded that differences between the metabolism of human and bull spermatozoa are quantitative and not qualitative. (In the past, human spermatozoa have sometimes been said to be unique in being unable to respire, in spite of containing cytochrome.) The pronounced difference between the aerobic and anaerobic heat production of bull and human seminal plasma is due to enzymatic reactions which take place in the latter. 23 mM-phosphate buffer, pH 7.4, inhibits the O 2 uptake of human spermatozoa.

scholarly journals NT-proBNP content in seminal plasma of ejaculate in normal conditions and in oligozooastenospermia

2020 ◽  
Vol 37 (4) ◽  
pp. 26-35
Author(s):  
Dmitriy Yu. Sosnin ◽  
Konstantin R. Galkovich ◽  
A. V. Krivtsov
Keyword(s):  
Seminal Plasma ◽  
Comparison Group ◽  
Solid Phase ◽  
Sperm Count ◽  
Test System ◽  
Mean Value ◽  
Main Group ◽  
Reproductive Age ◽  
System A ◽  
Human Fertility

Objective. To investigate the NT-proBNP concentration in ejaculate samples from healthy men and men with oligozooastenospermia. Materials and methods. The study included 52 men of reproductive age (34.4 3.9 years). The main group (n = 18) consisted of men with reduced ejaculate fertility, the comparison group (n = 34) consisted of men with normal levels of concentration and total sperm count. In seminal plasma, the concentration of NT-proBNP was investigated by solid-phase ELISA using the NT-proBNP-ELISA-BEST test system (A-9102) (Vector-Best, Russia). Results. The mean value of NT-proBNP content in the ejaculate (n = 52) was 29.498 pg / ml, the median concentration was 2.860 pg / ml and was characterized by high variability. In 62% (32 of 52) of the tested seminal plasma samples, the NT-proBNP value was below 20 pg / ml. The frequency of samples with such a low NT-proBNP content did not differ statistically significantly between the groups. In the main group, the number of such samples was 44 % (8 samples out of 18), and in the comparison group, 65 % (12 samples out of 34). Conclusions. When comparing the content of NT-proBNP in seminal plasma, no statistically significant differences were found between the groups. An analysis of the correlation between the concentration and the number of sperm in the ejaculate and the NT-proBNP content also did not reveal a statistically significant relationship. The possible role of natriuretic peptides in human fertility is discussed.

SOLUBLE FAS AND INTERLEUKIN-6 AND INTERLEUKIN-8 LEVELS IN SEMINAL PLASMA OF INFERTILE MEN

2003 ◽  
Vol 49 (6) ◽  
pp. 449-452 ◽  
Author(s):  
Y. Furuya ◽  
T. Akashi ◽  
H. Fuse
Keyword(s):  
Seminal Plasma ◽  
Interleukin 6 ◽  
Interleukin 8 ◽  
Soluble Fas ◽  
Infertile Men

SOLUBLE FAS AND INTERLEUKIN-6 AND INTERLEUKIN-8 LEVELS IN SEMINAL PLASMA OF INFERTILE MEN

2003 ◽  
Vol 49 (6) ◽  
pp. 449-452 ◽  
Author(s):  
Y. FURUYA ◽  
T. AKASHI ◽  
H. FUSE
Keyword(s):  
Seminal Plasma ◽  
Interleukin 6 ◽  
Interleukin 8 ◽  
Soluble Fas ◽  
Infertile Men

scholarly journals Oxidation of polymines by diamine oxidase from human seminal plasma

1975 ◽  
Vol 145 (2) ◽  
pp. 373-378 ◽  
Author(s):  
E Hölttä ◽  
P Pulkkinen ◽  
K Elfving ◽  
J Jänne
Keyword(s):  
Molecular Weight ◽  
Seminal Plasma ◽  
Gel Electrophoresis ◽  
Diamine Oxidase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Human Semen ◽  
Human Seminal Plasma ◽  
Ph Values

1. Diamine oxidase [amine-oxygen oxidoreductase (deaminating)(pyridoxal-containing), EC 1.4.3.6] was purified from human seminal plasma more than 1,700-fold. The enzyme appeared to be homogeneous on polyacrylamide-gel electrophoresis at two different pH values. 2. The general properties of the enzyme were comparable with those described for other diamine oxidases from different mammalian sources. The molecular weight of the enzyme was calculated to be about 182,000. 3. The enzyme had highest affinity for diamines, but polyamines spermidine and spermine were also degraded at concentrations that can be considered physiological in human semen. 3. The possible degradation of spermine by diamine oxidase in human semen in vivo may give rise to the formation of cytotoxic aldehydes that conceivably can influence the motility and survival of the spermatozoa.

scholarly journals Relationship Between Semen IL-6, IL-33 and Malondialdehyde Generation in Human Seminal Plasma and Spermatozoa

2021 ◽  
Author(s):  
Elena Moretti ◽  
Daniela Cerretani ◽  
Daria Noto ◽  
Cinzia Signorini ◽  
Francesca Iacoponi ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Sperm Function ◽  
Human Semen ◽  
Who Guidelines ◽  
Human Seminal Plasma ◽  
Pathological Conditions ◽  
Number Of Patients ◽  
The Relationship

AbstractCytokines are physiological seminal components and their abnormal levels, reported in different pathological conditions, negatively influence the sperm function. We analysed the relationship between interleukin (IL)-6 and IL-33 levels and lipid peroxidation (LPO), measured both in semen and sperm lysate, in 44 human semen samples. The semen analysis was performed following the WHO guidelines. Seminal IL-6 and IL-33 concentrations were assessed by ELISA and LPO was evaluated measuring malondialdehyde (MDA) both in seminal plasma and viable spermatozoa. Two small groups of patients with varicocele and infection were extrapolated from the cases analysed and the variables compared with those of a group of control. IL-33 levels were undetectable in all samples and IL-6 levels were positively correlated with both seminal and sperm MDA concentrations (p < 0.01) and negatively with sperm parameters (p < 0.01). Seminal and sperm MDA levels were both negatively correlated with sperm parameters (p < 0.01). IL-6 and semen MDA showed an exponential positive relationship, whereas MDA values measured in viable spermatozoa were low until IL-6 amount reached a concentration of >30 pg/mL, rising consistently. By comparing the variables in the groups, we confirmed that a high IL-6 concentration in the varicocele and infection groups was concomitant with an increase of seminal MDA levels, but also with MDA measured in viable spermatozoa, which represents the novelty of this study. We identified the IL-6 threshold, beyond which sperm MDA concentration rises concomitantly with the increase of IL-6 concentration. Other studies are needed, considering the increasing number of patients with different pathologies affecting male infertility.

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