scholarly journals Evaluation of In vitro and In vivo Antioxidant potential of Morinda reticulata Gamble Tubers in Wistar Albino Rats Subjected to CCl4 and Paracetamol induced Hepatotoxicity

2017 ◽  
Vol 28 (3) ◽  
pp. 147 ◽  
Author(s):  
Raju Asirvatham ◽  
Joshila Jose Usha
Keyword(s):  
Antioxidant Potential ◽  
Albino Rats ◽  
Wistar Albino Rats

In vivo phototoxic effects of a tetraethyleneglycol-substituted Zn phthalocyanine in tumor bearing rats at an enzymatic level

2020 ◽  
pp. A-H
Author(s):  
Muhammed Raşid Aykota ◽  
Sevda Yılmaz ◽  
Halil Erbiş ◽  
Nilgün Kabay ◽  
Sinem Tuncel Kostakoğlu ◽  
...  
Keyword(s):  
Albino Rats ◽  
Tetraethylene Glycol ◽  
Good Efficiency ◽  
Tumor Bearing ◽  
Oxidative Markers ◽  
Peripheral Position ◽  
Wistar Albino Rats

TEG-Pc, a Zn(II) phthalocyanine tetra substituted in non-peripheral position by tetraethylene glycol chains, proved to have good efficiency during in vitro photodynamic experiments. Its in vivo phototoxic effects have been investigated in tumor-bearing Wistar albino rats at an enzymatic level. The evolution of different oxidative markers are reviewed 6 h after the treatment.

scholarly journals Sciatic Nerve Regeneration in Wistar Albino Rats Evaluated by in vivo Conductivity and in vitro 1H NMR Relaxometry

2018 ◽  
Vol 64 (1) ◽  
pp. 22-27
Author(s):  
Anamaria Victoria Bumbu ◽  
Radu Fechete ◽  
Marcel Perian ◽  
Bogdan Septimiu Bumbu ◽  
Klara Brinzaniuc
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Conduction Velocity ◽  
1H Nmr ◽  
Albino Rats ◽  
Nmr Relaxometry ◽  
Wistar Albino Rats ◽  
1H Nmr Relaxometry

AbstractObjective: The aim of this study was to evaluate and quantify functional and structural nerve regeneration after reconstruction using either direct suture or silicon graft.Methods: Thirty-two adult Wistar Albino rats were divided in two equal groups. The left sciatic nerve was cross-sectioned and reconstructed using either direct suture (DS group) or a silicone graft (SG group). At 4, 6, 8 and 10 weeks two rats were randomly chosen from each group for in vivo measurement of nerve electric conductivity and subsequently sacrificed together with other two rats from the same group for in vitro 1H NMR relaxometry measurements. The T2 distributions were assigned to 1H located in different pools corresponding to the nerve structure.Results: In the injured nerve we observed a significant increase in the stimulation threshold and a decrease in conduction velocity when compared with the healthy nerve in both groups. Whereas the conduction velocity increased progressively from 4 to 10 weeks in the DS group, the opposite evolution was observed in the SG group. In both groups, the first two peaks corresponding to water bound to collagen and epineurium had smaller transverse relaxation times in the injured nerves, while there was no change in the peaks corresponding to perineurium and free water between healthy and injured nerves.Conclusions: Significant differences were observed between direct suture and nerve graft reconstructions from both a functional and structural point of view. In the case of direct suture reconstruction, the nerve was functionally healed at 10 weeks after injury.

Investigation on bio-properties and in-vivo antioxidant potential of carrageenans against alloxan induced oxidative stress in Wistar albino rats

2020 ◽  
Vol 151 ◽  
pp. 650-662 ◽  
Author(s):  
Muthusamy Sanjivkumar ◽  
Manohar Navin Chandran ◽  
Arumugampillai Manimehalai Suganya ◽  
Grasian Immanuel
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Potential ◽  
Albino Rats ◽  
Wistar Albino Rats

scholarly journals Hepatoprotective potential of Indigofera tirunelvelica Sanjappa: in vitro and in vivo studies on CCl4 induced wistar albino rats

2020 ◽  
Vol 11 (4) ◽  
pp. 6404-6410
Author(s):  
Subburayalu S ◽  
Asha KRT ◽  
Deepa Somanath ◽  
Palavesam A
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Albino Rats ◽  
Whole Plant ◽  
Wistar Albino Rats

The hepatoprotective efficiency of Indigofera tirunelvelica Sanjappa whole plant against CCl4 induced hepatotoxicity was examined. Rat hepatocyte monolayer culture and wistar albino rats were exercised as in vitro and in vivo screening models of protective agent for liver. In in vitro analyses, the whole plant ethanolic extract of Indigofera tirunelvelica Sanjappa were inspected. Silymarin was chosen as a standard treatement drug. In vitro, free radical scavenging property was also evaluated. In animal studies, hepatotoxicity was produced in Wistar albino rats by dispensing CCl4. The degree of hepatotoxicity was examined by determining the ranges of serum enzyme. The antioxidant parameters such as superoxide dismutase, catalase, reduced glutathione, and malondialdehyde of the hepatocytes were also evaluated. In in vitro studies, ethanol extract of I. tirunelvelica whole plant was identified to be the most active than other assessed extracts. Besides, whole plant ethanol extract of I. tirunelvelica was noticed to be rich in phenolic and flavonoids. It exhibited expressive free radical scavenging property versus diphenylpicryl hydrazyl (DPPH) and superoxide ion radicals. In the animals studies, whole plant ethanolic extract of I. tirunelvelica at a ranges of doses (100, 200 and 400 mg/kg body weight) revealed considerable amount of protection against CCl4 induced hepatotoxicity as evident by the protection of CCl4 induced changes biochemical parameters. The results of the present study suggested that the significant hepatoprotective property of whole plant ethanol extract of I. tirunelvelica against CCl4 induced hepatotoxicity and intimates its use as a potential medicinal drug for liver diseases.

scholarly journals Effect of antihyperlipidemic polyherbal formulation in high diet induced hyperlipidemia wistar albino rats. An in vitro - in vivo evaluation

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1531-1538
Author(s):  
Smitha Rani ◽  
Manju Sreedharan Nair Leelabai Amma ◽  
Sarada Nallani Chakravarthula
Keyword(s):  
Total Serum ◽  
Albino Rats ◽  
Total Serum Cholesterol ◽  
Low Density ◽  
Polyherbal Formulation ◽  
Anti Oxidant ◽  
In Vivo Analysis ◽  
Wistar Albino Rats

Hyperlipidemia is a prevalent global health problem, and it is linked to various cardiovascular disorders. The side effects of the current lipid-lowering drugs have increased the tendency to move toward traditional and alternative remedies. The study aims to Formulate and evaluate the Antilipidemic activity of Polyherbal formulations used as a traditional medicine in the Malabar area, Kerala by in vitro and in vivo methods. Further, the present study also compares the impact of seasonal variations on chemical contents of ingredient herbs of polyherbal medicine. They were analyzed for, anti-oxidant activity by DPPH and Nitric oxide method and In-vitro anti-cholesterol activity by cholesterol enzymatic endpoint method using simavastatin as a positive control. The formulation showing highest anti-oxidant and in- vitro Antilipidemic activity was selected for in-vivo analysis. Out of four formulations, PHF 1 shows low IC50  values in DPPH and Nitric oxide methods (250.45± 0.60, 985.40±5.59), respectively. The in-vitro anti-cholesterol activity showed a maximum % of inhibition for PHF1. Based on this PHF 1was selected for in vivo analysis. Acute toxicity was performed according to OECD guidelines. The antilipidemic activity was conducted by  Diet-induced hyperlipidemia model in Wistar albino rats, containing six animals in each group. All the groups except saline control received a high-fat diet for two weeks. The Polyherbal formulation (200 mg/kg & 400 mg/kg) showed significant (P<0.05) reduction in total serum cholesterol and lipid levels compared to the vehicle control group. This present study proved that Polyherbal formulation has Antilipidemic activity against the diet-induced hyperlipidemia model by reducing the total serum cholesterol (TC), triglycerides (TG), very low-density lipid (VLDL), low-density lipids(LDL) levels and increasing high-density lipid (HDL) level.

Synthesis, Characterization and in vivo Evaluation of PEGylated PPI Dendrimer for Safe and Prolonged Delivery of Insulin

2019 ◽  
Vol 9 (3) ◽  
pp. 248-263 ◽  
Author(s):  
Ashish K. Parashar ◽  
Preeti Patel ◽  
Arun K. Gupta ◽  
Neetesh K. Jain ◽  
Balak Das Kurmi
Keyword(s):  
Blood Glucose ◽  
Drug Release ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Albino Rats ◽  
Sustained Delivery ◽  
In Vivo Evaluation ◽  
Hemolytic Toxicity

Background: The present study was aimed at developing and exploring the use of PEGylated Poly (propyleneimine) dendrimers for the delivery of an anti-diabetic drug, insulin. Methods: For this study, 4.0G PPI dendrimer was synthesized by successive Michael addition and exhaustive amidation reactions, using ethylenediamine as the core and acrylonitrile as the propagating agent. Two different activated PEG moieties were employed for PEGylation of PPI dendrimers. Various physicochemical and physiological parameters UV, IR, NMR, TEM, DSC, drug entrapment, drug release, hemolytic toxicity and blood glucose level studies of both PEGylated and non- PEGylated dendritic systems were determined and compared. Results: PEGylation of PPI dendrimers caused increased solubilization of insulin in the dendritic framework as well as in PEG layers, reduced drug release and hemolytic toxicity as well as increased therapeutic efficacy with reduced side effects of insulin. These systems were found to be suitable for sustained delivery of insulin by in vitro and blood glucose-level studies in albino rats, without producing any significant hematological disturbances. Conclusion: Thus, surface modification of PPI dendrimers with PEG molecules has been found to be a suitable approach to utilize it as a safe and effective nano-carrier for drug delivery.

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