scholarly journals Activation of protease-activated receptors (PARs)-1 and -2 promotes alpha-smooth muscle actin expression and release of cytokines from human lung fibroblasts

2015 ◽  
Vol 3 (2) ◽  
pp. e12295 ◽  
Author(s):  
Nithiananthan Asokananthan ◽  
Rommel S. Lan ◽  
Peter T. Graham ◽  
Anthony J. Bakker ◽  
Ana Tokanović ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Human Lung ◽  
Smooth Muscle Actin ◽  
Lung Fibroblasts ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Protease Activated Receptors ◽  
Human Lung Fibroblasts ◽  
Actin Expression

scholarly journals MeCP2 epigenetically regulates alpha‐smooth muscle actin in human lung fibroblasts

2020 ◽  
Vol 121 (7) ◽  
pp. 3616-3625 ◽  
Author(s):  
Zheyi Xiang ◽  
Qingxian Zhou ◽  
Min Hu ◽  
Yan Y. Sanders
Keyword(s):  
Smooth Muscle ◽  
Human Lung ◽  
Smooth Muscle Actin ◽  
Lung Fibroblasts ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Human Lung Fibroblasts

Alpha smooth muscle actin expression in developing and adult human lung

1990 ◽  
Vol 44 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Kevin O. Leslie ◽  
John J. Mitchell ◽  
Janet L. Woodcock-Mitchell ◽  
Robert B. Low
Keyword(s):  
Smooth Muscle ◽  
Human Lung ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Adult Human ◽  
Actin Expression ◽  
Adult Human Lung

scholarly journals Alpha-Smooth Muscle Actin Expression Upregulates Fibroblast Contractile Activity

2001 ◽  
Vol 12 (9) ◽  
pp. 2730-2741 ◽  
Author(s):  
Boris Hinz ◽  
Giuseppe Celetta ◽  
James J. Tomasek ◽  
Giulio Gabbiani ◽  
Christine Chaponnier
Keyword(s):  
Smooth Muscle ◽  
Contractile Activity ◽  
Smooth Muscle Actin ◽  
Lung Fibroblasts ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Lattice Contraction ◽  
3T3 Fibroblasts ◽  
Cytoplasmic Actin ◽  
Actin Expression

To evaluate whether α-smooth muscle actin (α-SMA) plays a role in fibroblast contractility, we first compared the contractile activity of rat subcutaneous fibroblasts (SCFs), expressing low levels of α-SMA, with that of lung fibroblasts (LFs), expressing high levels of α-SMA, with the use of silicone substrates of different stiffness degrees. On medium stiffness substrates the percentage of cells producing wrinkles was similar to that of α-SMA–positive cells in each fibroblast population. On high stiffness substrates, wrinkle production was limited to a subpopulation of LFs very positive for α-SMA. In a second approach, we measured the isotonic contraction of SCF- and LF-populated attached collagen lattices. SCFs exhibited 41% diameter reduction compared with 63% by LFs. TGFβ1 increased α-SMA expression and lattice contraction by SCFs to the levels of LFs; TGFβ-antagonizing agents reduced α-SMA expression and lattice contraction by LFs to the level of SCFs. Finally, 3T3 fibroblasts transiently or permanently transfected with α-SMA cDNA exhibited a significantly higher lattice contraction compared with wild-type 3T3 fibroblasts or to fibroblasts transfected with α-cardiac and β- or γ-cytoplasmic actin. This took place in the absence of any change in smooth muscle or nonmuscle myosin heavy-chain expression. Our results indicate that an increased α-SMA expression is sufficient to enhance fibroblast contractile activity.

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