scholarly journals Role of Reactive Oxygen Species in Transforming Growth Factor - β1 - inuduced Fibronectin Secretion and α - Smooth Muscle Actin Expression in Human Lung Fibroblasts

2005 ◽  
Vol 58 (3) ◽  
pp. 267 ◽  
Author(s):  
Hunjoo Ha ◽  
Mi-Ra Yu ◽  
Soo-Taek Uh ◽  
Choon Sik Park ◽  
Hi Bahl Lee
Keyword(s):  
Transforming Growth Factor ◽  
Human Lung ◽  
Smooth Muscle Actin ◽  
Transforming Growth Factor Β1 ◽  
Lung Fibroblasts ◽  
Oxygen Species ◽  
Muscle Actin ◽  
Human Lung Fibroblasts ◽  
Actin Expression

Cell size, cell cycle, and α-smooth muscle actin expression by primary human lung fibroblasts

1998 ◽  
Vol 275 (5) ◽  
pp. L998-L1005 ◽  
Author(s):  
Bruce D. Uhal ◽  
Carlos Ramos ◽  
Iravati Joshi ◽  
Antonio Bifero ◽  
Annie Pardo ◽  
...  
Keyword(s):  
Human Lung ◽  
Smooth Muscle Actin ◽  
Lung Fibroblasts ◽  
Light Scatter ◽  
Forward Angle ◽  
Muscle Actin ◽  
Cell Sorter ◽  
Human Lung Fibroblasts ◽  
Group Iii

Primary human lung fibroblasts were separated into small ( group I), intermediate ( group II), and large ( group III) subpopulations by unit gravity sedimentation (1 G). The three subsets retained differences in cell size for up to 15 days of primary culture. Flow cytometric (fluorescence-activated cell sorter) measurements of forward-angle light scatter agreed well with fibroblast volume measured by image analysis and confirmed the utility of forward-angle light scatter for discriminating size subpopulations. Group II fibroblasts accumulated most rapidly by 8 days of culture and also contained the greatest proportion of S and G2/M phase cells as determined by fluorescence-activated cell sorter. Fibroblasts that were immunoreactive with antibodies to α-smooth muscle actin (α-SMA) were found only in group III. In situ end labeling of fragmented DNA detected apoptotic cells in both groups II and III, but double labeling for in situ end labeling and α-SMA revealed apoptotic cells in both the α-SMA-positive and -negative populations. These results demonstrate that primary human lung fibroblasts behave as predicted by classic models of cell cycle progression and differentiation. However, they do not support the hypothesis that the expression of α-actin is related to apoptosis. We also describe a simple and reproducible method for the high-yield isolation of human lung fibroblast subsets of differing proliferative potential and phenotype.

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