scholarly journals Interval‐induced metabolic perturbation determines tissue fluid shifts into skeletal muscle

2021 ◽  
Vol 9 (7) ◽  
Author(s):  
Mirko Mandić ◽  
Mikael F. Forsgren ◽  
Thobias Romu ◽  
Per Widholm ◽  
Patrik Sundblad ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Fluid ◽  
Metabolic Perturbation ◽  
Fluid Shifts

Fluid exchange in skeletal muscle with viscoelastic blood vessels

1987 ◽  
Vol 253 (6) ◽  
pp. H1548-H1556
Author(s):  
J. Lee ◽  
E. P. Salathe ◽  
G. W. Schmid-Schonbein
Keyword(s):  
Experimental Data ◽  
Mathematical Model ◽  
Skeletal Muscle ◽  
Blood Cells ◽  
Constant Pressure ◽  
Tissue Fluid ◽  
Flow State ◽  
Governing Equations ◽  
Fluid Exchange ◽  
Better Than

A mathematical model of capillary-tissue fluid exchange in a viscoelastic blood vessel is presented, and the Landis occlusion experiment is simulated. The model assumes that the fluid exchange is governed by Starling's law and that the protein and red blood cells are conserved in the capillary. Before occlusion, in the steady flow state, the pressure in the capillary decreases from the arterial to venous end due to viscous dissipation. After occlusion a constant pressure is established along the capillary. We assume the capillary to be distensible with viscoelastic wall properties. Immediately following occlusion an instantaneous distension of the capillary occurs. The vessel continues to expand viscoelastically while fluid is filtered for a period of several minutes, until it reaches an equilibrium state. A full numerical solution of the governing equations has been obtained. We use this model to compute the distance variation between two labeled erythrocytes as obtained in the Landis occlusion experiment and compare the results with experimental data obtained recently for the spinotrapezius muscle in our laboratory. The new model can fit the experimental data better than previous models that neglect the distensibility of the capillaries.

scholarly journals CX3CL1—a macrophage chemoattractant induced by a single bout of exercise in human skeletal muscle

2016 ◽  
Vol 310 (3) ◽  
pp. R297-R304 ◽  
Author(s):  
Anna Strömberg ◽  
Karl Olsson ◽  
Jacomijn P. Dijksterhuis ◽  
Eric Rullman ◽  
Gunnar Schulte ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Human Skeletal Muscle ◽  
Umbilical Vein ◽  
Vastus Lateralis ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Acute Monocytic Leukemia ◽  
Tissue Fluid ◽  
Mrna Levels

Monocytes/macrophages (MOs/MΦs) are suggested to be crucial for skeletal muscle repair and remodeling. This has been attributed to their proangiogenic potential, secretion of growth factors, and clearance of tissue debris. Skeletal muscle injury increases the number of MΦs in the tissue, and their importance for muscle regeneration has been supported by studies demonstrating that depletion of MOs/MΦs greatly impairs repair after muscle injury. Whether noninjurious exercise leads to induced expression of chemoattractants for MOs/MΦs is poorly investigated. To this end, we analyzed the expression of CX3CL1 (fractalkine), CCL2 (MCP-1), and CCL22 (MDC) in human skeletal muscle after a bout of exercise, all of which are established MO/MΦ chemotactic factors that are expressed by human myoblasts. Muscle biopsies from the musculus vastus lateralis were obtained up to 24 h after 1 h of cycle exercise in healthy individuals and in age-matched nonexercised controls. CX3CL1 increased at both the mRNA and protein level in human skeletal muscle after one bout of exercise. It was not possible to distinguish changes in CCL2 or CCL22 mRNA levels between biopsy vs. exercise effects, and the expression of CCL22 was very low. CX3CL1 mainly localized to the skeletal muscle endothelium, and it increased in human umbilical vein endothelial cells stimulated with tissue fluid from exercised muscle. CX3CL1 increased the expression of proinflammatory and proangiogenic factors in THP-1 monocytes (a human acute monocytic leukemia cell line) and in human primary myoblasts and myotubes. Altogether, this suggests that CX3CL1 participates in cross-talk mechanisms between endothelium and other muscle tissue cells and may promote a shift in the microenvironment toward a more regenerative milieu.

Tissue Fluid Shifts During Renal Arteriography With Conventional and Low Osmolality Agents

1983 ◽  
Vol 18 (4) ◽  
pp. 335-340 ◽  
Author(s):  
THOMAS W. MORRIS ◽  
PHILLIP P. HARNISH ◽  
KIMBERLY REECE ◽  
RICHARD W. KATZBERG
Keyword(s):  
Tissue Fluid ◽  
Fluid Shifts ◽  
Renal Arteriography

scholarly journals Modelling extracellular matrix and cellular contributions to whole muscle mechanics

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249601
Author(s):  
Ryan N. Konno ◽  
Nilima Nigam ◽  
James M. Wakeling
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Muscle Mechanics ◽  
Skeletal Muscle Tissue ◽  
Elastic Response ◽  
Muscle Fibres ◽  
Heterogeneous Structure ◽  
Tissue Fluid ◽  
Material Parameters ◽  
Whole Muscle

Skeletal muscle tissue has a highly complex and heterogeneous structure comprising several physical length scales. In the simplest model of muscle tissue, it can be represented as a one dimensional nonlinear spring in the direction of muscle fibres. However, at the finest level, muscle tissue includes a complex network of collagen fibres, actin and myosin proteins, and other cellular materials. This study shall derive an intermediate physical model which encapsulates the major contributions of the muscle components to the elastic response apart from activation-related along-fibre responses. The micro-mechanical factors in skeletal muscle tissue (eg. connective tissue, fluid, and fibres) can be homogenized into one material aggregate that will capture the behaviour of the combination of material components. In order to do this, the corresponding volume fractions for each type of material need to be determined by comparing the stress-strain relationship for a volume containing each material. This results in a model that accounts for the micro-mechanical features found in muscle and can therefore be used to analyze effects of neuro-muscular diseases such as cerebral palsy or muscular dystrophies. The purpose of this study is to construct a model of muscle tissue that, through choosing the correct material parameters based on experimental data, will accurately capture the mechanical behaviour of whole muscle. This model is then used to look at the impacts of the bulk modulus and material parameters on muscle deformation and strain energy-density distributions.

TISSUE FLUID SHIFTS DURING ARTERIOGRAPHY

1981 ◽  
Vol 16 (5) ◽  
pp. 429
Author(s):  
T. W. Morris ◽  
P. H. Harnish
Keyword(s):  
Tissue Fluid ◽  
Fluid Shifts

Isolation of interstitial fluid from skeletal muscle and subcutis in mice using a wick method

2004 ◽  
Vol 287 (5) ◽  
pp. H2085-H2090 ◽  
Author(s):  
Carl Erik Markhus ◽  
Helge Wiig
Keyword(s):  
Skeletal Muscle ◽  
Plasma Proteins ◽  
Interstitial Fluid ◽  
Colloid Osmotic Pressure ◽  
Tissue Fluid ◽  
Protein Extravasation ◽  
Plasma Protein Extravasation ◽  
Wick Technique ◽  
Region Plasma ◽  
Gain Access

Until recent years, mice were sparsely used in physiological experiments, and therefore, data on the basic cardiovascular parameters of mice are lacking. Our aim was to gain access to interstitial fluid and thereby study transcapillary fluid dynamics in this species. Using a modified wick method, we were able to isolate interstitial fluid from subcutis and skeletal muscle in mice. Three-stranded, dry, nylon wicks were inserted post mortem in an attempt to avoid local inflammation and thus eliminate protein extravasation and wick contamination. Colloid osmotic pressure (COP) was measured with a colloid osmometer for submicroliter samples and averaged (means ± SE) 18.7 ± 0.4 in plasma, 9.1 ± 0.4 in subcutis, and 12.3 ± 0.5 mmHg in muscle. HPLC of plasma and wick fluid showed similar patterns except for some minor peaks eluting in the <40-kDa region. Plasma protein extravasation as determined by 125I-labeled human serum albumin showed that contamination of wick fluid by plasma proteins was negligible (<2%). Capillary hyperfiltration induced by intravenous infusion of saline (10% of body wt) was reflected in tissue fluid isolated by wicks as shown by the average postinfusion COP values of 14.5 ± 0.6, 6.8 ± 0.3, and 7.7 ± 0.4 mmHg in plasma, subcutis, and muscle, respectively. We conclude that the wick technique can be easily adapted for use in mice and may represent a reliable method to isolate interstitial fluid and study transcapillary fluid flux in this species.

Malic Enzyme: Evidence for Two Molecular Forms in the Sarcoplasm of Fish Skeletal Muscle

1968 ◽  
Vol 25 (8) ◽  
pp. 1581-1589 ◽  
Author(s):  
Edith Gould
Keyword(s):  
Skeletal Muscle ◽  
Malic Enzyme ◽  
Molecular Forms ◽  
Free Form ◽  
Tissue Fluid ◽  
Migration Rates ◽  
Catalytically Active ◽  
A Subunit ◽  
Malic Enzyme Activity ◽  
Latent Activity

A latent form of malic enzyme activity (latent ME), which appears in the centrifuged tissue fluid of haddock skeletal muscle after the tissue has been frozen and thawed, appears also in the fluid of unfrozen haddock muscle after homogenization. It is more labile to refrigerated storage, ionizing radiation, and heat than is its normally soluble counterpart (free ME), and the two forms have demonstrably different electrophoretic migration rates. Although there is some evidence for catalytically active subunits for both forms, it has not yet been determined whether the latent activity is a true isoenzyme or a subunit of the free form.

Fluid shifts and muscle function in humans during acute simulated weightlessness

1983 ◽  
Vol 54 (4) ◽  
pp. 1003-1009 ◽  
Author(s):  
A. R. Hargens ◽  
C. M. Tipton ◽  
P. D. Gollnick ◽  
S. J. Mubarak ◽  
B. J. Tucker ◽  
...  
Keyword(s):  
Muscle Function ◽  
Interstitial Fluid ◽  
Plantar Flexion ◽  
Fluid Pressure ◽  
Lower Leg ◽  
Triceps Surae ◽  
Tissue Fluid ◽  
Simulated Weightlessness ◽  
Fluid Shifts ◽  
Reflex Time

Head-down tilt is considered an effective experimental model to simulate weightlessness. To determine the acute effects of simulated weightlessness on transcapillary fluid balance, tissue fluid shifts, muscle function, and triceps surae reflex time, eight supine subjects were tilted 5 degrees head down for 8 h. A cephalic fluid shift from the legs was indicated by facial edema, nasal congestion, increased urine flow, decreased creatinine excretion, reduced calf girth, and decreased lower leg volume. As measured by wick catheters inserted under local anesthesia, interstitial fluid pressure in the tibialis anterior muscle (4.6 +/- 0.6 mmHg) and subcutaneous tissue (0.6 +/- 0.5 mmHg) of the lower leg fell significantly to -2.8 +/- 0.5 and -3.8 +/- 0.4 mmHg, respectively. Other transcapillary pressures (capillary and interstitial fluid colloid osmotic pressures) were relatively unchanged. Needle-biopsy specimens, obtained just before and after tilt, indicated that total water content of soleus muscle was unchanged during 8 h of head-down tilt. After head-down tilt, isometric strength and isokinetic strength of the plantar flexors were unchanged. Triceps surae reflex time associated with plantar flexion movement slowed slightly after the tilt maneuver. Collectively these results demonstrated a dehydration effect of head-down tilt on muscle and subcutaneous tissues of the lower leg that may affect muscle function.

scholarly journals Acute high-intensity exercise and skeletal muscle mitochondrial respiratory function: role of metabolic perturbation

2021 ◽  
Vol 321 (5) ◽  
pp. R687-R698
Author(s):  
Matthew T. Lewis ◽  
Gregory M. Blain ◽  
Corey R. Hart ◽  
Gwenael Layec ◽  
Matthew J. Rossman ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
High Intensity ◽  
Respiratory Function ◽  
Complex I ◽  
High Intensity Exercise ◽  
Respiratory Capacity ◽  
Metabolic Perturbation ◽  
Exercise Induced ◽  
Mitochondrial Respiratory

Recently it was documented that fatiguing, high-intensity exercise resulted in a significant attenuation in maximal skeletal muscle mitochondrial respiratory capacity, potentially due to the intramuscular metabolic perturbation elicited by such intense exercise. With the utilization of intrathecal fentanyl to attenuate afferent feedback from group III/IV muscle afferents, permitting increased muscle activation and greater intramuscular metabolic disturbance, this study aimed to better elucidate the role of metabolic perturbation on mitochondrial respiratory function. Eight young, healthy males performed high-intensity cycle exercise in control (CTRL) and fentanyl-treated (FENT) conditions. Liquid chromatography-mass spectrometry and high-resolution respirometry were used to assess metabolites and mitochondrial respiratory function, respectively, pre- and postexercise in muscle biopsies from the vastus lateralis. Compared with CTRL, FENT yielded a significantly greater exercise-induced metabolic perturbation (PCr: −67% vs. −82%, Pi: 353% vs. 534%, pH: −0.22 vs. −0.31, lactate: 820% vs. 1,160%). Somewhat surprisingly, despite this greater metabolic perturbation in FENT compared with CTRL, with the only exception of respiratory control ratio (RCR) (−3% and −36%) for which the impact of FENT was significantly greater, the degree of attenuated mitochondrial respiratory capacity postexercise was not different between CTRL and FENT, respectively, as assessed by maximal respiratory flux through complex I (−15% and −33%), complex II (−36% and −23%), complex I + II (−31% and −20%), and state 3CI+CII control ratio (−24% and −39%). Although a basement effect cannot be ruled out, this failure of an augmented metabolic perturbation to extensively further attenuate mitochondrial function questions the direct role of high-intensity exercise-induced metabolite accumulation in this postexercise response.

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