Kinetics of batch microbial degradation of phenols by indigenous binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens

Abstract Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

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Bactericidal Activity, Absence of Serum Effect, and Time-Kill Kinetics of Ceftazidime-Avibactam against β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02894-14 ◽

2014 ◽

Vol 58 (9) ◽

pp. 5297-5305 ◽

Cited By ~ 55

Author(s):

Tiffany R. Keepers ◽

Marcela Gomez ◽

Chris Celeri ◽

Wright W. Nichols ◽

Kevin M. Krause

Keyword(s):

Pseudomonas Aeruginosa ◽

Human Serum ◽

Bactericidal Activity ◽

Minimum Bactericidal Concentration ◽

Wild Type ◽

Content Type ◽

Positive Isolate ◽

New Treatment ◽

Time Kill ◽

Kinetics Of

ABSTRACTAvibactam, a non-β-lactam β-lactamase inhibitor with activity against extended-spectrum β-lactamases (ESBLs), KPC, AmpC, and some OXA enzymes, extends the antibacterial activity of ceftazidime against most ceftazidime-resistant organisms producing these enzymes. In this study, the bactericidal activity of ceftazidime-avibactam against 18Pseudomonas aeruginosaisolates and 15Enterobacteriaceaeisolates, including wild-type isolates and ESBL, KPC, and/or AmpC producers, was evaluated. Ceftazidime-avibactam MICs (0.016 to 32 μg/ml) were lower than those for ceftazidime alone (0.06 to ≥256 μg/ml) against all isolates except for 2P. aeruginosaisolates (1blaVIM-positive isolate and 1blaOXA-23-positive isolate). The minimum bactericidal concentration/MIC ratios of ceftazidime-avibactam were ≤4 for all isolates, indicating bactericidal activity. Human serum and human serum albumin had a minimal effect on ceftazidime-avibactam MICs. Ceftazidime-avibactam time-kill kinetics were evaluated at low MIC multiples and showed time-dependent reductions in the number of CFU/ml from 0 to 6 h for all strains tested. A ≥3-log10decrease in the number of CFU/ml was observed at 6 h for allEnterobacteriaceae, and a 2-log10reduction in the number of CFU/ml was observed at 6 h for 3 of the 6P. aeruginosaisolates. Regrowth was noted at 24 h for some of the isolates tested in time-kill assays. These data demonstrate the potent bactericidal activity of ceftazidime-avibactam and support the continued clinical development of ceftazidime-avibactam as a new treatment option for infections caused byEnterobacteriaceaeandP. aeruginosa, including isolates resistant to ceftazidime by mechanisms dependent on avibactam-sensitive β-lactamases.

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Growth of Salmonella Enteritidis in the presence of quorum sensing signaling compounds produced by Pseudomonas aeruginosa

International Journal of Food Engineering ◽

10.1515/ijfe-2021-0089 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Weijia He ◽

Huamei Yang ◽

Xiang Wang ◽

Hongmei Li ◽

Qingli Dong

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Salmonella Enteritidis ◽

Culture Supernatant ◽

Lag Time ◽

Bacterial Communication ◽

Homoserine Lactones ◽

Positive Effects ◽

Kinetics Of

Abstract Quorum sensing (QS) can exist in food-related bacteria and potentially affect bacterial growth through acyl-homoserine lactones (AHLs). To verify the role of QS compounds in the cell-free supernatant, this study examined the effect of supernatant extracted from Pseudomonas aeruginosa culture on the growth kinetics of Salmonella Enteritidis. The results showed that the lag time (λ) of S. Enteritidis was apparently reduced (p < 0.05) under the influence of P. aeruginosa culture supernatant compared with the S. Enteritidis culture supernatant. HPLC-MS/MS test demonstrated that AHLs secreted by P. aeruginosa were mainly C14-HSL with a content of 85.71 μg/mL and a small amount of 3-oxo-C12-HSL. In addition, the commercially synthetic C14-HSL had positive effects on the growth of S. Enteritidis, confirming once again that the growth of S. Enteritidis was affected by AHL metabolized by other bacteria and the complexity of bacterial communication.

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Bioisomerization kinetics of γ-HCH and biokinetics of Pseudomonas aeruginosa degrading technical HCH

Biochemical Engineering Journal ◽

10.1016/j.bej.2006.12.015 ◽

2007 ◽

Vol 35 (1) ◽

pp. 12-19 ◽

Cited By ~ 16

Author(s):

Bharat Lodha ◽

Praveena Bhat ◽

M. Suresh Kumar ◽

Atul N. Vaidya ◽

Sandeep Mudliar ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Kinetics Of

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Mixed culture kinetics of stringent and relaxed Escherichia coli cells in glucose-limited chemostat

Zeitschrift für allgemeine Mikrobiologie ◽

10.1002/jobm.19840240210 ◽

1984 ◽

Vol 24 (2) ◽

pp. 113-117

Author(s):

D. Riesenberg ◽

F. Bergter

Keyword(s):

Escherichia Coli ◽

Mixed Culture ◽

Escherichia Coli Cells ◽

Kinetics Of

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Efficiency and Kinetics of Assam Crude Oil Degradation by Pseudomonas Aeruginosa AKS1 and Bacillus Sp. AKS2

10.21203/rs.3.rs-280894/v1 ◽

2021 ◽

Author(s):

Bobby Chettri ◽

Ningombam Anjana Singha ◽

Arvind Kumar Singh

Keyword(s):

Pseudomonas Aeruginosa ◽

Crude Oil ◽

Liquid Medium ◽

Half Life ◽

Liquid Culture ◽

Oil Degradation ◽

Biodegradation Rate ◽

Emulsification Index ◽

Crude Oil Degradation ◽

Kinetics Of

Abstract We report kinetics of Assam crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2, both isolated from Assam refinery sediments. The isolates exhibited appreciable degrees of hydrophobicity, emulsification index and biosurfactant production. Crude oil degradation efficiency of isolates was assessed in (1) liquid medium amended with 1% v/v crude oil and (2) microcosm sediments (125 mg crude oil/ 10 g sand). In liquid culture, the biodegradation rate (k) and half-life (t1/2) values were found to be 0.0383 day -1 and 18.09 days for P. aeruginosa AKS1, and 0.0204 day -1 and 33.97 days in case of Bacillus sp. AKS2. In microcosm sand sediments, the estimated biodegradation rate (k) and half-life (t 1/2) values were 0.0138 day -1 and 50 days for P. aeruginosa AKS1, and 0.0113 day -1 and 61.34 days in case of Bacillus sp. AKS2. The level of nutrient treatment in microcosm sand sediment was 125 µg N & 62.5 µg P/g sediment in case of P. aeruginosa AKS1 and 375 µg N & 37.5 µg P/g sediment in case of Bacillus sp. AKS2. In microcosms without inorganic nutrients, biodegradation rate (k) and half-life (t1/2) values were found to be 0.0069 day -1 and 100 days for P. aeruginosa AKS1 and for Bacillus sp. AKS2, the respective values were found to be 0.0046 day -1 and 150.68 days. Our data provides important information for predictive hydrocarbon degradation in liquid medium and contaminated sediments.

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KINETICS OF DIRHAMNOLIPIDS BIOSYNTHESIS AND RHAMNOSYLTRANSFERASE 2 ACTIVITY IN THE PRESENCE OF PSEUDOMONAS AERUGINOSA SIGNAL QUINOLONE

Microbiology&Biotechnology ◽

10.18524/2307-4663.2014.1(25).48202 ◽

2014 ◽

Vol 0 (1(25)) ◽

pp. 45-52

Author(s):

Мухліс Абедалабас ◽

М. Б. Галкін ◽

Є. Ю. Пахомова ◽

Т. О. Філіпова

Keyword(s):

Pseudomonas Aeruginosa ◽

Kinetics Of

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Role of single and mixed culture of Pseudomonas aeruginosa and Serratia ficaria in utilization of petroleum wastes of Dora- refineries.

Baghdad Science Journal ◽

10.21123/bsj.4.4.542-548 ◽

2007 ◽

Vol 4 (4) ◽

pp. 542-548

Author(s):

Baghdad Science Journal

Keyword(s):

Pseudomonas Aeruginosa ◽

Mixed Culture ◽

Bacterial Culture ◽

Bacterial Cultures ◽

Viable Cells ◽

Nacl Concentration ◽

Agitated Culture ◽

Growth Of Bacteria ◽

Better Than

The ability of single and mixed bacterial culture to utilize Dora-refineries petroleum wastes was compared. Pseudomonas aeruginosa and Serratia ficaria mixed culture consumed the wastes better than the single bacterial cultures. The highest log. number of viable cells in mixed culture was 6.842 , while in single bacterial cultures it was 6.683 and 5.631, respectively. after 3 days in API medium containing the refinery wastes. The effect of some environmental conditions on the degradation of petroleum wastes was studied included aeration , NaCl concentration , pH and temperature. The growth of bacteria in the agitated culture was higher than stagnant culture the log. of cell no. was 6.021 in the first culture. The highest log. of cell no. stagnant culture was 5.771. Pseudomonas aeruginosa AA22 and Serratia ficaria AA39 were able to grow in medium containing 5 , 7 % NaCl , they favorite pH 7. The mixed culture of the two bacteria grew well of 45 oC.

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